(Circular) (Six-base) MAPSORT of: pFN21ASDA1158.seq Check: 33 from: 1 to: 5374 pFN21ASDA1158 5374 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2387 2387 Size: 5374 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2653 2695 2653 Size: 42 5332 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 4821 5089 1632 Size: 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 2328 2604 4273 5250 1203 Size: 1125 276 1669 977 1327 Fragments arranged by size: 1669 1327 1125 977 276 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 2195 2198 2486 1166 Size: 1029 3 288 4054 Fragments arranged by size: 4054 1029 288 3 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2336 1184 Size: 1152 4222 AlwNI CAG_nnn'CTG Cuts at: 2085 3117 4689 2085 Size: 1032 1572 2770 Fragments arranged by size: 2770 1572 1032 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2683 2909 3061 5170 1493 Size: 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2219 2391 2699 1870 Size: 73 276 172 308 4545 Fragments arranged by size: 4545 308 276 172 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2292 2653 2695 4150 618 Size: 325 217 627 505 361 42 1455 1842 Fragments arranged by size: 1842 1455 627 505 361 325 217 42 BanII G_rGCy'C Cuts at: 1391 2282 2693 1391 Size: 891 411 4072 Fragments arranged by size: 4072 891 411 BbeI G_GCGC'C Cuts at: 2296 2296 Size: 5374 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2435 2515 928 Size: 971 536 80 3787 Fragments arranged by size: 3787 971 536 80 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3565 3599 1903 Size: 34 1628 34 3678 Fragments arranged by size: 3678 1628 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2588 3985 136 Size: 107 122 71 631 605 916 1397 1525 Fragments arranged by size: 1525 1397 916 631 605 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2112 3435 4591 1790 Size: 322 1323 1156 2573 Fragments arranged by size: 2573 1323 1156 322 BmgBI CAC'GTC Cuts at: 1318 2329 1318 Size: 1011 4363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 3105 4063 Size: 416 53 83 399 24 666 1022 958 Cuts at: 4063 442 Size: 1753 Fragments arranged by size: 1753 1022 958 666 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2490 4034 1239 Size: 1251 1544 2579 Fragments arranged by size: 2579 1544 1251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2497 2578 4037 882 Size: 617 998 81 1459 2219 Fragments arranged by size: 2219 1459 998 617 81 BsaAI yAC'GTr Cuts at: 493 1204 2671 3165 493 Size: 711 1467 494 2702 Fragments arranged by size: 2702 1467 711 494 BsaBI GATnn'nnATC Cuts at: 2510 5368 2510 Size: 2858 2516 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2293 3181 3563 4198 Size: 53 83 186 936 760 888 382 635 Cuts at: 4198 275 Size: 1451 Fragments arranged by size: 1451 936 888 760 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 1178 Size: 30 165 30 984 30 4135 Fragments arranged by size: 4135 984 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2477 1186 Size: 1006 285 4083 Fragments arranged by size: 4083 1006 285 BseYI C'CCAG_C Cuts at: 4577 5073 4577 Size: 496 4878 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 1163 Size: 573 414 4387 Fragments arranged by size: 4387 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2346 3435 3520 4591 2346 Size: 1089 85 1071 3129 Fragments arranged by size: 3129 1089 1071 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 BsmBI CGTCTCn'nnnn_ Cuts at: 2111 2111 Size: 5374 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2282 2346 2693 3435 3520 4591 Size: 399 322 170 64 347 742 85 1071 Cuts at: 4591 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 347 322 170 85 64 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 2502 3263 4993 2502 Size: 761 1730 2883 Fragments arranged by size: 2883 1730 761 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 2387 2729 844 Size: 1484 59 342 3489 Fragments arranged by size: 3489 1484 342 59 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2389 3869 4043 65 Size: 1183 1141 1480 174 1396 Fragments arranged by size: 1480 1396 1183 1141 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4018 4205 1700 Size: 110 2208 187 2869 Fragments arranged by size: 2869 2208 187 110 BsrGI T'GTAC_A Cuts at: 96 2508 96 Size: 2412 2962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 2060 3434 4446 2060 Size: 1374 1012 2988 Fragments arranged by size: 2988 1374 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2163 794 Size: 1369 4005 BstBI TT'CG_AA Cuts at: 2007 4178 2007 Size: 2171 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2200 2197 Size: 3 5371 BstYI r'GATC_y Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369 Size: 732 367 385 17 1452 86 12 346 Cuts at: 5369 1972 Size: 1977 Fragments arranged by size: 1977 1452 732 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2193 513 Size: 552 1128 3694 Fragments arranged by size: 3694 1128 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2306 2890 3684 3704 2306 Size: 584 794 20 3976 Fragments arranged by size: 3976 794 584 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DraIII CAC_nnn'GTG Cuts at: 2535 2535 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2260 2821 3710 Size: 54 1425 50 275 18 430 561 889 Cuts at: 3710 5339 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 561 430 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2580 3304 1353 Size: 812 415 724 3423 Fragments arranged by size: 3423 812 724 415 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2490 3437 4034 4821 5089 1239 Size: 393 858 947 597 787 268 1524 Fragments arranged by size: 1524 947 858 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2317 2317 Size: 5374 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2255 2255 Size: 5374 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2296 4521 1678 Size: 618 2225 2531 Fragments arranged by size: 2531 2225 618 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 4015 4047 4089 Size: 32 135 32 896 32 1515 32 42 Cuts at: 4089 4121 1373 Size: 32 2626 Fragments arranged by size: 2626 1515 896 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KasI G'GCGC_C Cuts at: 2292 2292 Size: 5374 KpnI G_GTAC'C Cuts at: 2657 2699 2657 Size: 42 5332 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 2604 5250 2604 Size: 2646 2728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4488 4672 1305 Size: 3183 184 2007 Fragments arranged by size: 3183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2262 10 Size: 54 1425 343 430 3122 Fragments arranged by size: 3122 1425 430 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 2198 2339 2486 2549 3332 3691 Size: 648 1029 3 141 147 63 783 359 Cuts at: 3691 3850 518 Size: 159 2042 Fragments arranged by size: 2042 1029 783 648 359 159 147 141 63 3 MspA1I CmG'CkG Cuts at: 1606 2790 3467 4615 4860 1606 Size: 1184 677 1148 245 2120 Fragments arranged by size: 2120 1184 1148 677 245 NarI GG'CG_CC Cuts at: 2293 2293 Size: 5374 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4822 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2732 4277 1132 Size: 97 1503 1545 2229 Fragments arranged by size: 2229 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5374 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 2272 1108 Size: 1164 4210 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2317 2317 Size: 5374 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PstI C_TGCA'G Cuts at: 830 2301 2325 2726 830 Size: 1471 24 401 3478 Fragments arranged by size: 3478 1471 401 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SbfI CC_TGCA'GG Cuts at: 2325 2726 2325 Size: 401 4973 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2212 2297 2321 2563 2722 3857 4538 Size: 219 1167 85 24 242 159 1135 681 Cuts at: 4538 4729 826 Size: 191 1471 Fragments arranged by size: 1471 1167 1135 681 242 219 191 159 85 24 SfoI GGC'GCC Cuts at: 2294 2294 Size: 5374 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2393 2701 1872 Size: 521 308 4545 Fragments arranged by size: 4545 521 308 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4379 4641 820 Size: 197 926 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2671 3165 493 Size: 2178 494 2702 Fragments arranged by size: 2702 2178 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2813 513 Size: 552 1748 3074 Fragments arranged by size: 3074 1748 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 3418 3571 3756 4201 325 Size: 1255 1821 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 3620 5332 Size: 274 80 33 51 1443 531 1112 1712 Cuts at: 5332 96 Size: 138 Fragments arranged by size: 1712 1443 1112 531 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5334 5366 5334 Size: 32 5342 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 2197 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2391 2699 1870 Size: 521 308 4545 Fragments arranged by size: 4545 521 308 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI