Restriction map for
pFN21ASDA1155
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AluI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI MwoI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 174 CCGC CCGC(-3/-1)
AflIII 101 ACATGT A^C[GA][CT]GT
AhdI 275 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 238 CCTCAGG CC^T[ACGT]AGG
AspEI 275 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 7 GGATCC G^GATCC
BbsI 202 GAAGAC GAAGAC(2/6)
BbvI 176 GCTGC GCAGC(8/12)
BmyI 67 GGGCAC G[AGT]GC[ACT]^C
BpuAI 202 GAAGAC GAAGAC(2/6)
BsaI 230 GAGACC GGTCTC(1/5)
BseRI 142 GAGGAG GAGGAG(10/8)
BsiYI 234 CCTGCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 234 CCTGCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 230 GAGAC GTCTC(1/5)
BsmFI 249 GGGAC GGGAC(10/14)
Bsp1286I 67 GGGCAC G[AGT]GC[ACT]^C
BspHI 294 TCATGA T^CATGA
BspLU11I 101 ACATGT A^CATGT
BspMI 233 ACCTGC ACCTGC(4/8)
BsrI 212 ACTGG ACTGG(1/-1)
BssSI 227 CACGAG CACGAG(-5/-1)
BstYI 7 GGATCC [GA]^GATC[CT]
Bsu36I 238 CCTCAGG CC^T[ACGT]AGG
DdeI 239 CTCAG C^T[ACGT]AG
DpnII 8 GATC ^GATC
DraIII 29 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 245 CCATGG C^C[GA][CT]GG
Eam1105I 275 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 118 GAAGAG CTCTTC(1/4)
Eco81I 238 CCTCAGG CC^T[ACGT]AGG
EcoNI 234 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 176 GCTGC GC^[ACGT]GC
HaeIII 243 GGCC GG^CC
HinfI 298 GATTC G^A[ACGT]TC
HphI 28 TCACC GGTGA(8/7)
ItaI 176 GCTGC GC^[ACGT]GC
Ksp632I 118 GAAGAG CTCTTC(1/4)
MaeIII 323 GTAAC ^GT[ACGT]AC
MboI 8 GATC ^GATC
MflI 7 GGATCC [GA]^GATC[CT]
MslI 102 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 174 CCGCTG C[AC]G^C[GT]G
NcoI 245 CCATGG C^CATGG
NdeII 8 GATC ^GATC
NlaIV 7 GGATCC GG[ACGT]^[ACGT]CC
NspI 101 ACATGT [GA]CATG^[CT]
PstI 151 CTGCAG CTGCA^G
RcaI 294 TCATGA T^CATGA
Sau3AI 8 GATC ^GATC
SfcI 151 CTGCAG C^T[GA][CT]AG
TfiI 298 GATTC G^A[AT]TC
TseI 176 GCTGC G^C[AT]GC
TspRI 285 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 54 CCAGGGGTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 7 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 99 GTAC GT^AC
366 GTAC
AlwI 8 GATCC GGATC(4/5)
7 GGATC
AvaII 51 GGACC G^G[AT]CC
171 GGACC
BssKI 54 CCAGG ^CC[ACGT]GG
355 CCAGG
BstF5I 107 GGATG GGATG(2/0)
195 GGATG
BstNI 54 CCAGG CC^[AT]GG
355 CCAGG
Cfr13I 51 GGACC G^G[ACGT]CC
171 GGACC
EcoRII 54 CCAGG ^CC[AT]GG
355 CCAGG
EcoT14I 245 CCATGG C^C[AT][AT]GG
341 CCAAGG
FokI 107 GGATG GGATG(9/13)
195 GGATG
MseI 61 TTAA T^TAA
268 TTAA
MvaI 54 CCAGG CC^[AT]GG
355 CCAGG
RsaI 99 GTAC GT^AC
366 GTAC
Sau96I 51 GGACC G^G[ACGT]CC
171 GGACC
ScrFI 54 CCAGG CC^[ACGT]GG
355 CCAGG
SfaNI 189 GATGC GCATC(5/9)
337 GATGC
StyI 245 CCATGG C^C[AT][AT]GG
341 CCAAGG
Tru9I 61 TTAA T^TAA
268 TTAA
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
BsaJI MboII MnlI NlaIII