Restriction map for
pFN21ASDA1135
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BspEI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CpoI Eam1105I Eco47III Eco81I
EcoO65I EcoRV FbaI FseI FspI
HindIII HpaI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1422 GTCGAC GT^[AC][GT]AC
AflIII 2210 ACATGT A^C[GA][CT]GT
Alw44I 665 GTGCAC G^TGCAC
ApaI 2177 GGGCCC GGGCC^C
ApaLI 665 GTGCAC G^TGCAC
BlpI 117 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 117 GCTGAGC GC^T[ACGT]AGC
BsmI 1660 GAATGC GAATGC(1/-1)
Bsp1407I 143 TGTACA T^GTACA
BspDI 469 ATCGAT AT^CGAT
BspLU11I 2210 ACATGT A^CATGT
BspMI 1332 GCAGGT ACCTGC(4/8)
BsrGI 143 TGTACA T^GTACA
BssHII 207 GCGCGC G^CGCGC
BstXI 482 CCAACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 117 GCTGAGC GC^T[ACGT]AGC
ClaI 469 ATCGAT AT^CGAT
DraI 1050 TTTAAA TTT^AAA
DraIII 238 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 277 GACGGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1707 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1642 GAATTC G^AATTC
EcoT14I 2093 CCATGG C^C[AT][AT]GG
EcoT22I 1690 ATGCAT ATGCA^T
HincII 1422 GTCGAC GT[CT]^[GA]AC
HindII 1422 GTCGAC GT[CT]^[GA]AC
MslI 2094 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2093 CCATGG C^CATGG
NsiI 1690 ATGCAT ATGCA^T
PflMI 1701 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 527 CTGCAG CTGCA^G
SacI 688 GAGCTC GAGCT^C
SalI 1422 GTCGAC G^TCGAC
ScaI 2234 AGTACT AGT^ACT
SfcI 527 CTGCAG C^T[GA][CT]AG
SspBI 143 TGTACA T^GTACA
SspI 1116 AATATT AAT^ATT
StuI 537 AGGCCT AGG^CCT
StyI 2093 CCATGG C^C[AT][AT]GG
Van91I 1701 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2066 CCAAAGAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 41 enzymes) #
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AcsI 1504 AAATTC [GA]^AATT[CT]
1642 GAATTC
AcyI 280 GGCGCC G[GA]^CG[CT]C
294 GGCGCC
AflII 733 CTTAAG C^TTAAG
856 CTTAAG
AlwNI 1600 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1702 CAGGTCCTG
ApoI 1504 AAATTC [GA]^AATT[CT]
1642 GAATTC
AvaI 76 CCCGGG C^[CT]CG[GA]G
107 CCCGGG
BalI 479 TGGCCA TGG^CCA
2165 TGGCCA
BbeI 280 GGCGCC GGCGC^C
294 GGCGCC
BfaI 457 CTAG C^TAG
770 CTAG
BfrI 733 CTTAAG C^TTAAG
856 CTTAAG
BglI 17 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1611 GCCTTCCAGGC
BsaHI 280 GGCGCC G[GA]^CG[CT]C
294 GGCGCC
BsgI 1340 CTGCAC GTGCAG(16/14)
1414 CTGCAC
BsmBI 1539 GAGACG CGTCTC(1/5)
1557 GAGACG
BsoBI 76 CCCGGG C^[CT]CG[GA]G
107 CCCGGG
BspHI 596 TCATGA T^CATGA
1011 TCATGA
BsrBI 1629 CCGCTC CCGCTC(-3/-3)
871 GAGCGG
BstAPI 667 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1599 GCAGTTCCTGC
BstF5I 1020 CATCC GGATG(2/0)
930 GGATG
FokI 1020 CATCC GGATG(9/13)
930 GGATG
HaeII 280 GGCGCC [GA]GCGC^[CT]
294 GGCGCC
Hin1I 280 GGCGCC G[GA]^CG[CT]C
294 GGCGCC
KasI 280 GGCGCC G^GCGCC
294 GGCGCC
MaeI 457 CTAG C^TAG
770 CTAG
MaeII 1176 ACGT A^CGT
1592 ACGT
MaeIII 286 GTCAC ^GT[ACGT]AC
1234 GTGAC
MluNI 479 TGGCCA TGG^CCA
2165 TGGCCA
MscI 479 TGGCCA TGG^CCA
2165 TGGCCA
NaeI 85 GCCGGC GCC^GGC
2147 GCCGGC
NarI 280 GGCGCC GG^CGCC
294 GGCGCC
NgoMIV 85 GCCGGC G^CCGGC
2147 GCCGGC
NspI 1205 GCATGT [GA]CATG^[CT]
2210 ACATGT
PleI 1267 GAGTC GAGTC(4/5)
1396 GAGTC
PpuMI 1703 AGGTCCT [GA]G^G[AT]CC[CT]
1923 GGGACCT
RcaI 596 TCATGA T^CATGA
1011 TCATGA
SapI 784 GAAGAGC GCTCTTC(1/4)
1459 GAAGAGC
SfoI 280 GGCGCC GGC^GCC
294 GGCGCC
SmaI 76 CCCGGG CCC^GGG
107 CCCGGG
Tsp45I 286 GTCAC ^GT[GC]AC
1234 GTGAC
XmaCI 76 CCCGGG C^CCGGG
107 CCCGGG
XmaI 76 CCCGGG C^CCGGG
107 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrFI BsrI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII