(Circular) (Six-base) MAPSORT of: pFN21ASDA1135.seq Check: 7018 from: 1 to: 6997 pFN21ASDA1135 6997 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3346 3346 Size: 6997 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4807 278 Size: 53 83 186 936 3271 2468 Fragments arranged by size: 3271 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3446 4340 1937 Size: 1509 894 4594 Fragments arranged by size: 4594 1509 894 Acc65I G'GTAC_C Cuts at: 4318 4318 Size: 6997 AclI AA'CG_TT Cuts at: 1563 5124 5497 1563 Size: 3561 373 3063 Fragments arranged by size: 3561 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3501 4251 5060 6444 6712 1632 Size: 1869 750 809 1384 268 1917 Fragments arranged by size: 1917 1869 1384 809 750 268 AflII C'TTAA_G Cuts at: 820 1017 2756 2879 820 Size: 197 1739 123 4938 Fragments arranged by size: 4938 1739 197 123 AflIII A'CryG_T Cuts at: 1203 4233 5896 6873 1203 Size: 3030 1663 977 1327 Fragments arranged by size: 3030 1663 1327 977 AgeI A'CCGG_T Cuts at: 5828 5828 Size: 6997 AhdI GACnn_n'nnGTC Cuts at: 5726 5726 Size: 6997 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6997 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3378 3914 1184 Size: 2194 536 4267 Fragments arranged by size: 4267 2194 536 AlwNI CAG_nnn'CTG Cuts at: 3628 3730 4740 6312 3628 Size: 102 1010 1572 4313 Fragments arranged by size: 4313 1572 1010 102 ApaI G_GGCC'C Cuts at: 4204 4204 Size: 6997 ApaLI G'TGCA_C Cuts at: 2688 5054 6210 2688 Size: 2366 1156 3475 Fragments arranged by size: 3475 2366 1156 ApoI r'AATT_y Cuts at: 1493 3527 3665 4306 4532 4684 6793 1493 Size: 2034 138 641 226 152 2109 1697 Fragments arranged by size: 2109 2034 1697 641 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 5551 160 Size: 872 4519 1606 Fragments arranged by size: 4519 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 AvaI C'yCGr_G Cuts at: 1870 1943 2099 2130 4322 1870 Size: 73 156 31 2192 4545 Fragments arranged by size: 4545 2192 156 73 31 BamHI G'GATC_C Cuts at: 4327 4694 4327 Size: 367 6630 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2317 3358 4318 5773 Size: 325 217 627 516 14 1041 960 1455 Cuts at: 5773 618 Size: 1842 Fragments arranged by size: 1842 1455 1041 960 627 516 325 217 14 BanII G_rGCy'C Cuts at: 1391 2100 2129 2715 3391 4204 4316 1391 Size: 709 29 586 676 813 112 4072 Fragments arranged by size: 4072 813 709 676 586 112 29 BbeI G_GCGC'C Cuts at: 2307 2321 2307 Size: 14 6983 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6026 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5188 5222 1903 Size: 34 3251 34 3678 Fragments arranged by size: 3678 3251 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4889 6099 4889 Size: 1210 5787 BclI T'GATC_A Cuts at: 1548 1548 Size: 6997 BfrBI ATG'CAT Cuts at: 3715 3715 Size: 6997 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2046 3640 5608 Size: 107 122 71 631 605 374 1594 1968 Cuts at: 5608 136 Size: 1525 Fragments arranged by size: 1968 1594 1525 631 605 374 122 107 71 BglII A'GATC_T Cuts at: 4287 6992 4287 Size: 2705 4292 BlpI GC'TnA_GC Cuts at: 2141 4414 2141 Size: 2273 4724 Bme1580I G_kGCm'C Cuts at: 1790 2692 2833 4204 5058 6214 1790 Size: 902 141 1371 854 1156 2573 Fragments arranged by size: 2573 1371 1156 902 854 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6997 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2345 2836 3671 Size: 416 53 83 399 24 928 491 835 Cuts at: 3671 4728 5686 442 Size: 1057 958 1753 Fragments arranged by size: 1753 1057 958 928 835 491 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6997 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2314 3515 4064 4129 5657 1239 Size: 929 146 1201 549 65 1528 2579 Fragments arranged by size: 2579 1528 1201 929 549 146 65 Bpu10I CC'TnA_GC Cuts at: 2028 2028 Size: 6997 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3680 5122 5987 6285 3680 Size: 1442 865 298 4392 Fragments arranged by size: 4392 1442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5660 882 Size: 617 4161 2219 Fragments arranged by size: 4161 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4294 4788 493 Size: 711 3090 494 2702 Fragments arranged by size: 3090 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6991 6991 Size: 6997 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 2318 4804 5186 Size: 53 83 186 936 771 14 2486 382 Cuts at: 5186 5821 275 Size: 635 1451 Fragments arranged by size: 2486 1451 936 771 635 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 5430 5828 6102 6249 6660 5430 Size: 398 274 147 411 5767 Fragments arranged by size: 5767 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 3111 3141 3488 Size: 30 165 30 777 30 901 30 347 Cuts at: 3488 3518 1178 Size: 30 4657 Fragments arranged by size: 4657 901 777 347 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2201 3203 3371 4030 1186 Size: 979 36 1002 168 659 4153 Fragments arranged by size: 4153 1002 979 659 168 36 BseYI C'CCAG_C Cuts at: 3552 6200 6696 3552 Size: 2648 496 3853 Fragments arranged by size: 3853 2648 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3348 3422 1163 Size: 573 1612 74 4738 Fragments arranged by size: 4738 1612 573 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 2277 2328 4447 5208 6236 Size: 275 186 120 156 51 2119 761 1028 Cuts at: 6236 1540 Size: 2301 Fragments arranged by size: 2301 2119 1028 761 275 186 156 120 51 BsiHKAI G_wGCw'C Cuts at: 2692 2715 3034 3881 4115 5058 5143 6214 2692 Size: 23 319 847 234 943 85 1071 3475 Fragments arranged by size: 3475 1071 943 847 319 234 85 23 BsmI GAATG_Cn' Cuts at: 3689 4508 4601 3689 Size: 819 93 6085 Fragments arranged by size: 6085 819 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3556 3574 3556 Size: 18 6979 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2129 2692 2715 2833 3034 3391 Size: 399 310 29 563 23 118 201 357 Cuts at: 3391 3881 4115 4204 4316 5058 5143 6214 1391 Size: 490 234 89 112 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 563 490 399 357 310 234 201 118 112 89 85 29 23 BspEI T'CCGG_A Cuts at: 6660 6660 Size: 6997 BspHI T'CATG_A Cuts at: 2619 3034 4886 6616 2619 Size: 415 1852 1730 3000 Fragments arranged by size: 3000 1852 1730 415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3346 4352 844 Size: 2502 1006 3489 Fragments arranged by size: 3489 2502 1006 BsrBI CCG'CTC Cuts at: 1394 1823 2896 3654 4884 1394 Size: 429 1073 758 1230 3507 Fragments arranged by size: 3507 1230 1073 758 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2536 3219 4026 5492 5666 65 Size: 1183 1288 683 807 1466 174 1396 Fragments arranged by size: 1466 1396 1288 1183 807 683 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2108 3871 4170 5641 5828 1700 Size: 110 298 1763 299 1471 187 2869 Fragments arranged by size: 2869 1763 1471 299 298 187 110 BsrGI T'GTAC_A Cuts at: 96 2166 96 Size: 2070 4927 BssHII G'CGCG_C Cuts at: 1925 2230 1925 Size: 305 6692 BssSI C'ACGA_G Cuts at: 5057 6069 5057 Size: 1012 5985 BstAPI GCAn_nnn'nTGC Cuts at: 794 2696 3628 794 Size: 1902 932 4163 Fragments arranged by size: 4163 1902 932 BstBI TT'CG_AA Cuts at: 2007 5801 2007 Size: 3794 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6997 BstXI CCAn_nnnn'nTGG Cuts at: 2512 2512 Size: 6997 BstYI r'GATC_y Cuts at: 1972 2154 2283 3059 4287 4327 4694 5079 5096 Size: 182 129 776 1228 40 367 385 17 Cuts at: 5096 6548 6634 6646 6992 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1228 776 385 367 346 182 129 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2060 2090 2322 3113 3119 4116 Size: 552 957 38 30 232 791 6 997 Cuts at: 4116 513 Size: 3394 Fragments arranged by size: 3394 997 957 791 552 232 38 30 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2799 487 Size: 683 56 1573 4685 Fragments arranged by size: 4685 1573 683 56 BtsI GCAGTG_nn' Cuts at: 2079 3999 4513 5307 5327 2079 Size: 1920 514 794 20 3749 Fragments arranged by size: 3749 1920 794 514 20 ClaI AT'CG_AT Cuts at: 1554 2493 1554 Size: 939 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6962 DraI TTT'AAA Cuts at: 3075 4301 3075 Size: 1226 5771 DraIII CAC_nnn'GTG Cuts at: 2266 2266 Size: 6997 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2306 6004 809 Size: 507 990 3698 1802 Fragments arranged by size: 3698 1802 990 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2274 2325 Size: 54 1425 50 275 18 288 156 51 Cuts at: 2325 2502 3116 3218 3399 4013 4188 4444 5333 Size: 177 614 102 181 614 175 256 889 Cuts at: 5333 6962 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 614 614 288 275 256 181 177 175 156 102 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2118 2274 2325 4444 1537 Size: 275 306 156 51 2119 4090 Fragments arranged by size: 4090 2119 306 275 156 51 EarI CTCTTCn'nnn_ Cuts at: 1353 2802 3477 3933 4927 1353 Size: 1449 675 456 994 3423 Fragments arranged by size: 3423 1449 994 675 456 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5584 5958 6104 387 Size: 1064 138 3995 374 146 1280 Fragments arranged by size: 3995 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2713 2713 Size: 6997 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2314 3501 3515 4064 4129 4251 Size: 393 536 146 1187 14 549 65 122 Cuts at: 4251 5060 5657 6444 6712 1239 Size: 809 597 787 268 1524 Fragments arranged by size: 1524 1187 809 787 597 549 536 393 268 146 122 65 14 EcoNI CCTnn'n_nnAGG Cuts at: 3734 3734 Size: 6997 EcoO109I rG'GnC_Cy Cuts at: 3727 3947 4200 4247 3727 Size: 220 253 47 6477 Fragments arranged by size: 6477 253 220 47 EcoRI G'AATT_C Cuts at: 3665 4306 4684 3665 Size: 641 378 5978 Fragments arranged by size: 5978 641 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2771 3820 2771 Size: 1049 5948 FspI TGC'GCA Cuts at: 5503 5503 Size: 6997 HaeII r_GCGC'y Cuts at: 1678 2307 2321 6144 1678 Size: 629 14 3823 2531 Fragments arranged by size: 3823 2531 629 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3488 3520 5638 5670 5712 Size: 32 135 32 1916 32 2118 32 42 Cuts at: 5712 5744 1373 Size: 32 2626 Fragments arranged by size: 2626 2118 1916 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3447 4341 4587 669 Size: 988 281 1509 894 246 3079 Fragments arranged by size: 3079 1509 988 894 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6997 HpaI GTT'AAC Cuts at: 4587 4587 Size: 6997 KasI G'GCGC_C Cuts at: 2303 2317 2303 Size: 14 6983 KpnI G_GTAC'C Cuts at: 4322 4322 Size: 6997 MfeI C'AATT_G Cuts at: 4596 4596 Size: 6997 MluI A'CGCG_T Cuts at: 6873 6873 Size: 6997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6111 6295 1305 Size: 4806 184 2007 Fragments arranged by size: 4806 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2504 4190 10 Size: 54 1425 343 672 1686 2817 Fragments arranged by size: 2817 1686 1425 672 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4121 4955 5314 5473 518 Size: 648 2955 834 359 159 2042 Fragments arranged by size: 2955 2042 834 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2092 2324 3370 4413 5090 6238 6483 Size: 456 30 232 1046 1043 677 1148 245 Cuts at: 6483 1606 Size: 2120 Fragments arranged by size: 2120 1148 1046 1043 677 456 245 232 30 NaeI GCC'GGC Cuts at: 2110 4172 2110 Size: 2062 4935 NarI GG'CG_CC Cuts at: 2304 2318 2304 Size: 14 6983 NcoI C'CATG_G Cuts at: 513 1065 2022 4116 513 Size: 552 957 2094 3394 Fragments arranged by size: 3394 2094 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6997 NgoMIV G'CCGG_C Cuts at: 2108 4170 2108 Size: 2062 4935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6997 NotI GC'GGCC_GC Cuts at: 4444 4444 Size: 6997 NsiI A_TGCA'T Cuts at: 3717 3717 Size: 6997 NspI r_CATG'y Cuts at: 1132 1229 3232 4237 4355 5900 1132 Size: 97 2003 1005 118 1545 2229 Fragments arranged by size: 2229 2003 1545 1005 118 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6997 PciI A'CATG_T Cuts at: 4233 5896 4233 Size: 1663 5334 PflMI CCAn_nnn'nTGG Cuts at: 1108 3730 1108 Size: 2622 4375 PfoI T'CCnGG_A Cuts at: 3492 3492 Size: 6997 PmeI GTTT'AAAC Cuts at: 4301 4301 Size: 6997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 5066 5098 6579 6611 2011 Size: 32 3023 32 1481 32 2397 Fragments arranged by size: 3023 2397 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3727 3947 3727 Size: 220 6777 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6997 PsiI TTA'TAA Cuts at: 2424 4567 2424 Size: 2143 4854 PspOMI G'GGCC_C Cuts at: 4200 4200 Size: 6997 PstI C_TGCA'G Cuts at: 830 2554 4349 830 Size: 1724 1795 3478 Fragments arranged by size: 3478 1795 1724 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6997 SacI G_AGCT'C Cuts at: 2715 2715 Size: 6997 SacII CC_GC'GG Cuts at: 2063 2093 2325 2063 Size: 30 232 6735 Fragments arranged by size: 6735 232 30 SalI G'TCGA_C Cuts at: 1936 3445 4339 1936 Size: 1509 894 4594 Fragments arranged by size: 4594 1509 894 SapI GCTCTTCn'nnn_ Cuts at: 2802 3477 2802 Size: 675 6322 SbfI CC_TGCA'GG Cuts at: 4349 4349 Size: 6997 ScaI AGT'ACT Cuts at: 4259 5245 6957 4259 Size: 986 1712 4299 Fragments arranged by size: 4299 1712 986 SfcI C'TryA_G Cuts at: 826 1045 2550 4345 5480 6161 6352 826 Size: 219 1505 1795 1135 681 191 1471 Fragments arranged by size: 1795 1505 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2305 2319 2305 Size: 14 6983 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6997 SmaI CCC'GGG Cuts at: 1872 2101 2132 4324 1872 Size: 229 31 2192 4545 Fragments arranged by size: 4545 2192 229 31 SmlI C'TyrA_G Cuts at: 820 1017 1943 2756 2879 3659 5101 6002 6264 Size: 197 926 813 123 780 1442 901 262 Cuts at: 6264 820 Size: 1553 Fragments arranged by size: 1553 1442 926 901 813 780 262 197 123 SnaBI TAC'GTA Cuts at: 493 4294 4788 493 Size: 3801 494 2702 Fragments arranged by size: 3801 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6997 SphI G_CATG'C Cuts at: 1132 4355 1132 Size: 3223 3774 SspI AAT'ATT Cuts at: 5 52 3141 4921 5 Size: 47 3089 1780 2081 Fragments arranged by size: 3089 2081 1780 47 StuI AGG'CCT Cuts at: 1517 2562 1517 Size: 1045 5952 StyI C'CwwG_G Cuts at: 513 1065 2022 4116 4436 513 Size: 552 957 2094 320 3074 Fragments arranged by size: 3074 2094 957 552 320 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2592 5024 5041 5194 5379 5824 325 Size: 1255 1012 2432 17 153 185 445 1498 Fragments arranged by size: 2432 1498 1255 1012 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2901 4257 Size: 274 80 33 51 1443 189 735 1356 Cuts at: 4257 5243 6955 96 Size: 986 1712 138 Fragments arranged by size: 1712 1443 1356 986 735 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 3111 3143 4267 4299 6957 6989 2180 Size: 32 899 32 1124 32 2658 32 2188 Fragments arranged by size: 2658 2188 1124 899 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6997 XbaI T'CTAG_A Cuts at: 4333 4333 Size: 6997 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4096 4096 Size: 6997 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6997 XmaI C'CCGG_G Cuts at: 1870 2099 2130 4322 1870 Size: 229 31 2192 4545 Fragments arranged by size: 4545 2192 229 31 XmnI GAAnn'nnTTC Cuts at: 5126 5126 Size: 6997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4805 276 Size: 53 83 186 936 3271 2468 Fragments arranged by size: 3271 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SrfI SwaI