Restriction map for
pFN21ASDA1129
(Circular) (Six-base) MAPSORT of: pFN21ASDA1129.seq Check: 581 from: 1 to: 6490
pFN21ASDA1129 6490 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2519 3032 3746 2519
Size: 513 714 5263
Fragments arranged by size:
5263 714 513
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3149 4300 278
Size: 53 83 186 936 1613 1151 2468
Fragments arranged by size:
2468 1613 1151 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3833 1937
Size: 1896 4594
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2391 2631 4553 5937 6205 1632
Size: 759 240 1922 1384 268 1917
Fragments arranged by size:
1922 1917 1384 759 268 240
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 2268 2734 3066 3594 5389 6366 1203
Size: 1065 466 332 528 1795 977 1327
Fragments arranged by size:
1795 1327 1065 977 528 466 332
AgeI A'CCGG_T
Cuts at: 2997 5321 2997
Size: 2324 4166
AhdI GACnn_n'nnGTC
Cuts at: 5219 5219
Size: 6490
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2553 1184
Size: 1369 5121
AlwNI CAG_nnn'CTG
Cuts at: 2059 2323 2389 3391 4233 5805 2059
Size: 264 66 1002 842 1572 2744
Fragments arranged by size:
2744 1572 1002 842 264 66
ApaLI G'TGCA_C
Cuts at: 4547 5703 4547
Size: 1156 5334
ApoI r'AATT_y
Cuts at: 1493 2582 3799 4025 4177 6286 1493
Size: 1089 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 1089 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 3815 1870
Size: 73 1872 4545
Fragments arranged by size:
4545 1872 73
AvrII C'CTAG_G
Cuts at: 2756 2756
Size: 6490
BamHI G'GATC_C
Cuts at: 3662 3820 4187 3662
Size: 158 367 5965
Fragments arranged by size:
5965 367 158
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2950 3811 5266 618
Size: 325 217 627 1163 861 1455 1842
Fragments arranged by size:
1842 1455 1163 861 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 3636 3809 1391
Size: 660 1585 173 4072
Fragments arranged by size:
4072 1585 660 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3465 5101 136
Size: 107 122 71 631 605 1793 1636 1525
Fragments arranged by size:
1793 1636 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6485 6485
Size: 6490
BlpI GC'TnA_GC
Cuts at: 2045 3015 3485 3907 2045
Size: 970 470 422 4628
Fragments arranged by size:
4628 970 470 422
Bme1580I G_kGCm'C
Cuts at: 1790 4551 5707 1790
Size: 2761 1156 2573
Fragments arranged by size:
2761 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2176 2308 1318
Size: 858 132 5500
Fragments arranged by size:
5500 858 132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3191 4221 5179
Size: 416 53 83 399 24 1774 1030 958
Cuts at: 5179 442
Size: 1753
Fragments arranged by size:
1774 1753 1030 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2892 5150 1239
Size: 1653 2258 2579
Fragments arranged by size:
2579 2258 1653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4615 5480 5778 4615
Size: 865 298 5327
Fragments arranged by size:
5327 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5153 882
Size: 617 3654 2219
Fragments arranged by size:
3654 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3067 3439 3787 4281 493
Size: 711 1863 372 348 494 2702
Fragments arranged by size:
2702 1863 711 494 372 348
BsaBI GATnn'nnATC
Cuts at: 2297 6484 2297
Size: 4187 2303
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3146 4297 4679 5314
Size: 53 83 186 936 1613 1151 382 635
Cuts at: 5314 275
Size: 1451
Fragments arranged by size:
1613 1451 1151 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2997 4923 5321 5595 5742 6153 2997
Size: 1926 398 274 147 411 3334
Fragments arranged by size:
3334 1926 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2984 3014 1178
Size: 30 165 30 1581 30 4654
Fragments arranged by size:
4654 1581 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2843 3005 1186
Size: 1657 162 4671
Fragments arranged by size:
4671 1657 162
BseYI C'CCAG_C
Cuts at: 2069 3415 5693 6189 2069
Size: 1346 2278 496 2370
Fragments arranged by size:
2370 2278 1346 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2497 2632 3642 1163
Size: 573 761 135 1010 4011
Fragments arranged by size:
4011 1010 761 573 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3940 4701 5729 1540
Size: 275 186 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2051 2715 3179 3198 3398 3455 3740 4551 4636
Size: 664 464 19 200 57 285 811 85
Cuts at: 4636 5707 2051
Size: 1071 2834
Fragments arranged by size:
2834 1071 811 664 464 285 200 85
57 19
BsmI GAATG_Cn'
Cuts at: 2129 4001 4094 2129
Size: 1872 93 4525
Fragments arranged by size:
4525 1872 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2715 3179 3198 3398 3455 3636
Size: 399 261 664 464 19 200 57 181
Cuts at: 3636 3740 3809 4551 4636 5707 1391
Size: 104 69 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 464 399 261 200
181 104 85 69 57 19
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 2928 4379 6109 2928
Size: 1451 1730 3309
Fragments arranged by size:
3309 1730 1451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2519 3032 3746 3845 844
Size: 1675 513 714 99 3489
Fragments arranged by size:
3489 1675 714 513 99
BsrBI CCG'CTC
Cuts at: 1394 1823 4377 1394
Size: 429 2554 3507
Fragments arranged by size:
3507 2554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3150 4985 5159 65
Size: 1183 1902 1835 174 1396
Fragments arranged by size:
1902 1835 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2997 3600 5134 5321 1700
Size: 110 1187 603 1534 187 2869
Fragments arranged by size:
2869 1534 1187 603 187 110
BsrGI T'GTAC_A
Cuts at: 96 2503 96
Size: 2407 4083
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3761 794
Size: 2967 3523
BstBI TT'CG_AA
Cuts at: 2007 5294 2007
Size: 3287 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstXI CCAn_nnnn'nTGG
Cuts at: 2479 2479
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 3662 3820 4187 4572 4589 6041 6127 6139
Size: 1690 158 367 385 17 1452 86 12
Cuts at: 6139 6485 1972
Size: 346 1977
Fragments arranged by size:
1977 1690 1452 385 367 346 158 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3075 513
Size: 552 957 1053 3928
Fragments arranged by size:
3928 1053 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2267 487
Size: 683 56 1041 4710
Fragments arranged by size:
4710 1041 683 56
BtsI GCAGTG_nn'
Cuts at: 2974 3095 3222 3325 3469 4006 4800 4820 2974
Size: 121 127 103 144 537 794 20 4644
Fragments arranged by size:
4644 794 537 144 127 121 103 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DraIII CAC_nnn'GTG
Cuts at: 2218 2218
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2768 5497 809
Size: 507 1452 2729 1802
Fragments arranged by size:
2729 1802 1452 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2944 3061 3937
Size: 54 1425 50 275 18 1114 117 876
Cuts at: 3937 4826 6455 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1114 889 876 275 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3937 1537
Size: 275 2125 4090
Fragments arranged by size:
4090 2125 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2367 2646 3530 4420 1353
Size: 1014 279 884 890 3423
Fragments arranged by size:
3423 1014 890 884 279
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3328 3458 3760 5077 5451 5597
Size: 1064 138 1739 130 302 1317 374 146
Cuts at: 5597 387
Size: 1280
Fragments arranged by size:
1739 1317 1280 1064 374 302 146 138
130
EcoICRI GAG'CTC
Cuts at: 2049 2049
Size: 6490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2391 2631 2892 4553 5150 5937 6205
Size: 393 759 240 261 1661 597 787 268
Cuts at: 6205 1239
Size: 1524
Fragments arranged by size:
1661 1524 787 759 597 393 268 261
240
EcoNI CCTnn'n_nnAGG
Cuts at: 2831 2921 2831
Size: 90 6400
EcoRI G'AATT_C
Cuts at: 3799 4177 3799
Size: 378 6112
EcoRV GAT'ATC
Cuts at: 2622 2622
Size: 6490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2603 2603
Size: 6490
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 3172 5637 1678
Size: 1494 2465 2531
Fragments arranged by size:
2531 2465 1494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2733 2765 3647 3679 5131
Size: 32 135 32 1161 32 882 32 1452
Cuts at: 5131 5163 5205 5237 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1452 1161 882 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2107 3834 4080 669
Size: 988 281 169 1727 246 3079
Fragments arranged by size:
3079 1727 988 281 246 169
HindIII A'AGCT_T
Cuts at: 748 2790 748
Size: 2042 4448
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 2473 4089 2473
Size: 1616 4874
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2764 3457 3628 3749 5604 5788 1305
Size: 1459 693 171 121 1855 184 2007
Fragments arranged by size:
2007 1855 1459 693 184 171 121
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3063 10
Size: 54 1425 343 1231 3437
Fragments arranged by size:
3437 1425 1231 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2933 3069 3593 4448 4807 4966 518
Size: 648 1767 136 524 855 359 159 2042
Fragments arranged by size:
2042 1767 855 648 524 359 159 136
MspA1I CmG'CkG
Cuts at: 1606 2164 3191 3496 3906 4583 5731 5976 1606
Size: 558 1027 305 410 677 1148 245 2120
Fragments arranged by size:
2120 1148 1027 677 558 410 305 245
NaeI GCC'GGC
Cuts at: 3602 3602
Size: 6490
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4981
Fragments arranged by size:
4981 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NgoMIV G'CCGG_C
Cuts at: 3600 3600
Size: 6490
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NspI r_CATG'y
Cuts at: 1132 1229 2272 2738 3163 3598 3848 5393 1132
Size: 97 1043 466 425 435 250 1545 2229
Fragments arranged by size:
2229 1545 1043 466 435 425 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 2268 2734 3594 5389 2268
Size: 466 860 1795 3369
Fragments arranged by size:
3369 1795 860 466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2218 3282 3429 1108
Size: 1110 1064 147 4169
Fragments arranged by size:
4169 1110 1064 147
PfoI T'CCnGG_A
Cuts at: 3556 3556
Size: 6490
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PmlI CAC'GTG
Cuts at: 3067 3067
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 6072 6104 4559
Size: 32 1481 32 4945
Fragments arranged by size:
4945 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2909 1613
Size: 1296 5194
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PstI C_TGCA'G
Cuts at: 830 2237 2415 2454 2529 3842 830
Size: 1407 178 39 75 1313 3478
Fragments arranged by size:
3478 1407 1313 178 75 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 2164 3496 2164
Size: 1332 5158
SacI G_AGCT'C
Cuts at: 2051 2051
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SapI GCTCTTCn'nnn_
Cuts at: 3530 3530
Size: 6490
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 3238 3689 4738 6450 3238
Size: 451 1049 1712 3278
Fragments arranged by size:
3278 1712 1049 451
SexAI A'CCwGG_T
Cuts at: 2604 2604
Size: 6490
SfcI C'TryA_G
Cuts at: 826 1045 2233 2411 2450 2525 3262 3838 4973
Size: 219 1188 178 39 75 737 576 1135
Cuts at: 4973 5654 5845 826
Size: 681 191 1471
Fragments arranged by size:
1471 1188 1135 737 681 576 219 191
178 75 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4594 5495 5757 820
Size: 197 926 2651 901 262 1553
Fragments arranged by size:
2651 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3787 4281 493
Size: 3294 494 2702
Fragments arranged by size:
3294 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3163 3848 1132
Size: 2031 685 3774
Fragments arranged by size:
3774 2031 685
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6490
StyI C'CwwG_G
Cuts at: 513 1065 2022 2756 2888 2922 2955 3929 513
Size: 552 957 734 132 34 33 974 3074
Fragments arranged by size:
3074 974 957 734 552 132 34 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2968 3314 4517 4534 4687 4872 5317
Size: 1255 1388 346 1203 17 153 185 445
Cuts at: 5317 325
Size: 1498
Fragments arranged by size:
1498 1388 1255 1203 445 346 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2503 3236 3408
Size: 274 80 33 51 1443 526 733 172
Cuts at: 3408 3687 4736 6448 96
Size: 279 1049 1712 138
Fragments arranged by size:
1712 1443 1049 733 526 279 274 172
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2594 2626 2984 3016 6450 6482 2594
Size: 32 358 32 3434 32 2602
Fragments arranged by size:
3434 2602 358 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 2129 2583 4619 2129
Size: 454 2036 4000
Fragments arranged by size:
4000 2036 454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3147 4298 276
Size: 53 83 186 936 1613 1151 2468
Fragments arranged by size:
2468 1613 1151 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I FseI
FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI