Restriction map for
pFN21ASDA1125
(Circular) (Six-base) MAPSORT of: pFN21ASDA1125.seq Check: 8125 from: 1 to: 8287
pFN21ASDA1125 8287 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5351 6097 278
Size: 53 83 186 936 3815 746 2468
Fragments arranged by size:
3815 2468 936 746 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5630 1937
Size: 3693 4594
Acc65I G'GTAC_C
Cuts at: 5608 5608
Size: 8287
AclI AA'CG_TT
Cuts at: 1563 6414 6787 1563
Size: 4851 373 3063
Fragments arranged by size:
4851 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2897 3382 4112 5298 6350 7734 8002 1632
Size: 1265 485 730 1186 1052 1384 268 1917
Fragments arranged by size:
1917 1384 1265 1186 1052 730 485 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8090
AflIII A'CryG_T
Cuts at: 1203 5504 7186 8163 1203
Size: 4301 1682 977 1327
Fragments arranged by size:
4301 1682 1327 977
AgeI A'CCGG_T
Cuts at: 4303 7118 4303
Size: 2815 5472
AhdI GACnn_n'nnGTC
Cuts at: 7016 7016
Size: 8287
AleI CACnn'nnGTG
Cuts at: 1166 3399 4274 4679 4736 1166
Size: 2233 875 405 57 4717
Fragments arranged by size:
4717 2233 875 405 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2949 3281 1184
Size: 1765 332 6190
Fragments arranged by size:
6190 1765 332
AlwNI CAG_nnn'CTG
Cuts at: 2252 2964 4001 4939 5008 5157 5222 6030 7602
Size: 712 1037 938 69 149 65 808 1572
Cuts at: 7602 2252
Size: 2937
Fragments arranged by size:
2937 1572 1037 938 808 712 149 69
65
ApaLI G'TGCA_C
Cuts at: 3341 6344 7500 3341
Size: 3003 1156 4128
Fragments arranged by size:
4128 3003 1156
ApoI r'AATT_y
Cuts at: 1493 3003 5596 5822 5974 8083 1493
Size: 1510 2593 226 152 2109 1697
Fragments arranged by size:
2593 2109 1697 1510 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6841 160
Size: 872 5809 1606
Fragments arranged by size:
5809 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8287
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 4717 5293 5612 1870
Size: 73 400 2374 576 319 4545
Fragments arranged by size:
4545 2374 576 400 319 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5509 5542 5509
Size: 33 8254
BamHI G'GATC_C
Cuts at: 5617 5984 5617
Size: 367 7920
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4343 5608 7063 618
Size: 325 217 627 2556 1265 1455 1842
Fragments arranged by size:
2556 1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2337 3353 4658 4956 5291 5606 1391
Size: 946 1016 1305 298 335 315 4072
Fragments arranged by size:
4072 1305 1016 946 335 315 298
BbeI G_GCGC'C
Cuts at: 4347 4347
Size: 8287
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3873 4185 4578 928
Size: 971 1974 312 393 4637
Fragments arranged by size:
4637 1974 971 393 312
BbvCI CC'TCA_GC
Cuts at: 4777 4910 5229 5247 4777
Size: 133 319 18 7817
Fragments arranged by size:
7817 319 133 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6478 6512 1903
Size: 34 4541 34 3678
Fragments arranged by size:
4541 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6179 7389 6179
Size: 1210 7077
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8287
BfrBI ATG'CAT
Cuts at: 2436 2537 2436
Size: 101 8186
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2663 6898 136
Size: 107 122 71 631 605 991 4235 1525
Fragments arranged by size:
4235 1525 991 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8282 8282
Size: 8287
BlpI GC'TnA_GC
Cuts at: 3617 5704 3617
Size: 2087 6200
Bme1580I G_kGCm'C
Cuts at: 1790 3281 3345 6348 7504 1790
Size: 1491 64 3003 1156 2573
Fragments arranged by size:
3003 2573 1491 1156 64
BmgBI CAC'GTC
Cuts at: 1318 3358 3387 3975 4365 4665 1318
Size: 2040 29 588 390 300 4940
Fragments arranged by size:
4940 2040 588 390 300 29
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3938 4409 5206
Size: 416 53 83 399 24 2521 471 797
Cuts at: 5206 6018 6976 442
Size: 812 958 1753
Fragments arranged by size:
2521 1753 958 812 797 471 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8287
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5392 5428 5392
Size: 36 8251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4023 4916 5030 5075 5383 6947 1239
Size: 2784 893 114 45 308 1564 2579
Fragments arranged by size:
2784 2579 1564 893 308 114 45
Bpu10I CC'TnA_GC
Cuts at: 4029 4777 4910 5190 5229 5247 4029
Size: 748 133 280 39 18 7069
Fragments arranged by size:
7069 748 280 133 39 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 6412 7277 7575 2480
Size: 3932 865 298 3192
Fragments arranged by size:
3932 3192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2435 5078 6950 882
Size: 617 936 2643 1872 2219
Fragments arranged by size:
2643 2219 1872 936 617
BsaAI yAC'GTr
Cuts at: 493 1204 3070 5584 6078 493
Size: 711 1866 2514 494 2702
Fragments arranged by size:
2702 2514 1866 711 494
BsaBI GATnn'nnATC
Cuts at: 8281 8281
Size: 8287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4344 5348 5408 6094
Size: 53 83 186 936 2811 1004 60 686
Cuts at: 6094 6476 7111 275
Size: 382 635 1451
Fragments arranged by size:
2811 1451 1004 936 686 635 382 186
83 60 53
BsaWI w'CCGG_w
Cuts at: 4303 6720 7118 7392 7539 7950 4303
Size: 2417 398 274 147 411 4640
Fragments arranged by size:
4640 2417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3335 3365 3533 3563 5467
Size: 30 165 30 1932 30 168 30 1904
Cuts at: 5467 5497 1178
Size: 30 3968
Fragments arranged by size:
3968 1932 1904 168 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3425 3498 3974 5217 5281 5404 5534 1186
Size: 2239 73 476 1243 64 123 130 3939
Fragments arranged by size:
3939 2239 1243 476 130 123 73 64
BseYI C'CCAG_C
Cuts at: 2090 2295 3173 7490 7986 2090
Size: 205 878 4317 496 2391
Fragments arranged by size:
4317 2391 878 496 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2632 4484 4925 5191 1163
Size: 573 896 1852 441 266 4259
Fragments arranged by size:
4259 1852 896 573 441 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3771 4461 5737 6498 7526 1540
Size: 275 186 1770 690 1276 761 1028 2301
Fragments arranged by size:
2301 1770 1276 1028 761 690 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 3329 3345 3440 3780 4311 4733 4956 5196
Size: 992 16 95 340 531 422 223 240
Cuts at: 5196 5291 5300 6348 6433 7504 2337
Size: 95 9 1048 85 1071 3120
Fragments arranged by size:
3120 1071 1048 992 531 422 340 240
223 95 95 85 16 9
BsmI GAATG_Cn'
Cuts at: 2438 2537 2629 5798 5891 2438
Size: 99 92 3169 93 4834
Fragments arranged by size:
4834 3169 99 93 92
BsmBI CGTCTCn'nnnn_
Cuts at: 4100 5339 5399 4100
Size: 1239 60 6988
Fragments arranged by size:
6988 1239 60
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 3281 3329 3345 3353 3440 3780
Size: 399 547 944 48 16 8 87 340
Cuts at: 3780 4311 4658 4733 4956 5196 5291 5300 5606
Size: 531 347 75 223 240 95 9 306
Cuts at: 5606 6348 6433 7504 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 944 742 547 531 399 347
340 306 240 223 95 87 85 75
48 16 9 8
BspEI T'CCGG_A
Cuts at: 7950 7950
Size: 8287
BspHI T'CATG_A
Cuts at: 6176 7906 6176
Size: 1730 6557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 2917 3675 4767 5642 844
Size: 1655 418 758 1092 875 3489
Fragments arranged by size:
3489 1655 1092 875 758 418
BsrBI CCG'CTC
Cuts at: 1394 1823 4317 5020 6174 1394
Size: 429 2494 703 1154 3507
Fragments arranged by size:
3507 2494 1154 703 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6782 6956 65
Size: 1183 5534 174 1396
Fragments arranged by size:
5534 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3179 3557 4303 5025 6931 7118 1700
Size: 110 1369 378 746 722 1906 187 2869
Fragments arranged by size:
2869 1906 1369 746 722 378 187 110
BsrGI T'GTAC_A
Cuts at: 96 2609 96
Size: 2513 5774
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8287
BssSI C'ACGA_G
Cuts at: 6347 7359 6347
Size: 1012 7275
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4531 794
Size: 3737 4550
BstBI TT'CG_AA
Cuts at: 2007 7091 2007
Size: 5084 3203
BstEII G'GTnAC_C
Cuts at: 1184 4375 4681 4708 1184
Size: 3191 306 27 4763
Fragments arranged by size:
4763 3191 306 27
BstXI CCAn_nnnn'nTGG
Cuts at: 4276 4678 4276
Size: 402 7885
BstYI r'GATC_y
Cuts at: 1972 2047 3246 5617 5984 6369 6386 7838 7924
Size: 75 1199 2371 367 385 17 1452 86
Cuts at: 7924 7936 8282 1972
Size: 12 346 1977
Fragments arranged by size:
2371 1977 1452 1199 385 367 346 86
75 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3417 4335 4371 4986 513
Size: 552 957 1395 918 36 615 3814
Fragments arranged by size:
3814 1395 957 918 615 552 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4683 4750 5017 487
Size: 683 56 3457 67 267 3757
Fragments arranged by size:
3757 3457 683 267 67 56
BtsI GCAGTG_nn'
Cuts at: 3183 4770 5105 5488 5803 6597 6617 3183
Size: 1587 335 383 315 794 20 4853
Fragments arranged by size:
4853 1587 794 383 335 315 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2841 2876 3927 3962 5353 5388 529
Size: 35 2277 35 1051 35 1391 35 3428
Fragments arranged by size:
3428 2277 1391 1051 35 35 35 35
DraI TTT'AAA
Cuts at: 5591 5591
Size: 8287
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2927 7294 809
Size: 507 1611 4367 1802
Fragments arranged by size:
4367 1802 1611 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 3768 4458
Size: 54 1425 50 275 18 283 1655 690
Cuts at: 4458 5322 5734 6623 8252 8
Size: 864 412 889 1629 43
Fragments arranged by size:
1655 1629 1425 889 864 690 412 283
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3768 4458 5734 1537
Size: 275 1956 690 1276 4090
Fragments arranged by size:
4090 1956 1276 690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3340 4324 5486 5531 6217 1353
Size: 1987 984 1162 45 686 3423
Fragments arranged by size:
3423 1987 1162 984 686 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 5006 6874 7248 7394 387
Size: 1064 138 2723 694 1868 374 146 1280
Fragments arranged by size:
2723 1868 1280 1064 694 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 4954 5289 2335
Size: 2619 335 5333
Fragments arranged by size:
5333 2619 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2897 3382 4023 4112 4916 5030 5075
Size: 393 1265 485 641 89 804 114 45
Cuts at: 5075 5298 5383 6350 6947 7734 8002 1239
Size: 223 85 967 597 787 268 1524
Fragments arranged by size:
1524 1265 967 804 787 641 597 485
393 268 223 114 89 85 45
EcoNI CCTnn'n_nnAGG
Cuts at: 5012 5012
Size: 8287
EcoO109I rG'GnC_Cy
Cuts at: 2961 2961
Size: 8287
EcoRI G'AATT_C
Cuts at: 5596 5974 5596
Size: 378 7909
EcoRV GAT'ATC
Cuts at: 2029 4604 2029
Size: 2575 5712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3052 3238 3052
Size: 186 8101
FspI TGC'GCA
Cuts at: 6793 6793
Size: 8287
HaeII r_GCGC'y
Cuts at: 1678 3180 3673 4347 5331 7434 1678
Size: 1502 493 674 984 2103 2531
Fragments arranged by size:
2531 2103 1502 984 674 493
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 3533 3565 3833
Size: 32 135 32 467 32 1462 32 268
Cuts at: 3833 3865 4522 4554 4588 4620 6928 6960 7002
Size: 32 657 32 34 32 2308 32 42
Cuts at: 7002 7034 1373
Size: 32 2626
Fragments arranged by size:
2626 2308 1462 657 467 268 135 42
34 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4750 5631 5877 669
Size: 988 281 2812 881 246 3079
Fragments arranged by size:
3079 2812 988 881 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8287
HpaI GTT'AAC
Cuts at: 5877 5877
Size: 8287
KasI G'GCGC_C
Cuts at: 4343 4343
Size: 8287
KpnI G_GTAC'C
Cuts at: 5612 5612
Size: 8287
MfeI C'AATT_G
Cuts at: 5886 5886
Size: 8287
MluI A'CGCG_T
Cuts at: 8163 8163
Size: 8287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3282 3378 5040 5123 5453 5457 7401 7585
Size: 1977 96 1662 83 330 4 1944 184
Cuts at: 7585 1305
Size: 2007
Fragments arranged by size:
2007 1977 1944 1662 330 184 96 83
4
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2115 10
Size: 54 1425 343 283 6182
Fragments arranged by size:
6182 1425 343 283 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 3399 4274 4679 4736 6245 6604
Size: 648 1296 937 875 405 57 1509 359
Cuts at: 6604 6763 518
Size: 159 2042
Fragments arranged by size:
2042 1509 1296 937 875 648 405 359
159 57
MspA1I CmG'CkG
Cuts at: 1606 3694 4337 4468 4781 4793 5053 5354 5703
Size: 2088 643 131 313 12 260 301 349
Cuts at: 5703 6380 7528 7773 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 2088 1148 677 643 349 313 301
260 245 131 12
NaeI GCC'GGC
Cuts at: 3181 3181
Size: 8287
NarI GG'CG_CC
Cuts at: 4344 4344
Size: 8287
NcoI C'CATG_G
Cuts at: 513 1065 2022 3417 4986 513
Size: 552 957 1395 1569 3814
Fragments arranged by size:
3814 1569 1395 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8287
NgoMIV G'CCGG_C
Cuts at: 3179 3179
Size: 8287
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8287
NotI GC'GGCC_GC
Cuts at: 5734 5734
Size: 8287
NsiI A_TGCA'T
Cuts at: 2438 2539 2438
Size: 101 8186
NspI r_CATG'y
Cuts at: 1132 1229 4525 5645 7190 1132
Size: 97 3296 1120 1545 2229
Fragments arranged by size:
3296 2229 1545 1120 97
PasI CC'CwG_GG
Cuts at: 1348 4591 5281 1348
Size: 3243 690 4354
Fragments arranged by size:
4354 3243 690
PciI A'CATG_T
Cuts at: 7186 7186
Size: 8287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2599 1108
Size: 1491 6796
PfoI T'CCnGG_A
Cuts at: 3426 5453 3426
Size: 2027 6260
PmeI GTTT'AAAC
Cuts at: 5591 5591
Size: 8287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6356 6388 7869 7901 6356
Size: 32 1481 32 6742
Fragments arranged by size:
6742 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8287
PsiI TTA'TAA
Cuts at: 2703 5857 2703
Size: 3154 5133
PspXI vC'TCGA_Gb
Cuts at: 5293 5293
Size: 8287
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5138 5170 5138
Size: 32 8255
PstI C_TGCA'G
Cuts at: 830 2062 5639 830
Size: 1232 3577 3478
Fragments arranged by size:
3577 3478 1232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8287
PvuII CAG'CTG
Cuts at: 3694 4781 5053 5354 3694
Size: 1087 272 301 6627
Fragments arranged by size:
6627 1087 301 272
SacI G_AGCT'C
Cuts at: 2337 4956 5291 2337
Size: 2619 335 5333
Fragments arranged by size:
5333 2619 335
SacII CC_GC'GG
Cuts at: 4338 4338
Size: 8287
SalI G'TCGA_C
Cuts at: 1936 5629 1936
Size: 3693 4594
SapI GCTCTTCn'nnn_
Cuts at: 4324 5531 4324
Size: 1207 7080
SbfI CC_TGCA'GG
Cuts at: 5639 5639
Size: 8287
ScaI AGT'ACT
Cuts at: 6535 8247 6535
Size: 1712 6575
SfcI C'TryA_G
Cuts at: 826 1045 2058 5635 6770 7451 7642 826
Size: 219 1013 3577 1135 681 191 1471
Fragments arranged by size:
3577 1471 1135 1013 681 219 191
SfoI GGC'GCC
Cuts at: 4345 4345
Size: 8287
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8287
SgrAI Cr'CCGG_yG
Cuts at: 1700 4303 1700
Size: 2603 5684
SmaI CCC'GGG
Cuts at: 1872 2345 5614 1872
Size: 473 3269 4545
Fragments arranged by size:
4545 3269 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2495 5293 6391 7292 7554 820
Size: 197 926 552 2798 1098 901 262 1553
Fragments arranged by size:
2798 1553 1098 926 901 552 262 197
SnaBI TAC'GTA
Cuts at: 493 5584 6078 493
Size: 5091 494 2702
Fragments arranged by size:
5091 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8287
SphI G_CATG'C
Cuts at: 1132 5645 1132
Size: 4513 3774
SspI AAT'ATT
Cuts at: 5 52 6211 5
Size: 47 6159 2081
Fragments arranged by size:
6159 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8287
StyI C'CwwG_G
Cuts at: 513 1065 2022 3417 4986 5437 5726 513
Size: 552 957 1395 1569 451 289 3074
Fragments arranged by size:
3074 1569 1395 957 552 451 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3148 3616 5225 6314 6331 6484 6669
Size: 1255 1568 468 1609 1089 17 153 185
Cuts at: 6669 7114 325
Size: 445 1498
Fragments arranged by size:
1609 1568 1498 1255 1089 468 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2609 4806 6533
Size: 274 80 33 51 1443 632 2197 1727
Cuts at: 6533 8245 96
Size: 1712 138
Fragments arranged by size:
2197 1727 1712 1443 632 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3335 3367 3634 3666 4401 4433 5186 5218 5467
Size: 32 267 32 735 32 753 32 249
Cuts at: 5467 5499 8247 8279 3335
Size: 32 2748 32 3343
Fragments arranged by size:
3343 2748 753 735 267 249 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2211 1316
Size: 895 7392
XbaI T'CTAG_A
Cuts at: 5623 5623
Size: 8287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2391 3055 3424 4675 2391
Size: 664 369 1251 6003
Fragments arranged by size:
6003 1251 664 369
XhoI C'TCGA_G
Cuts at: 1943 5293 1943
Size: 3350 4937
XmaI C'CCGG_G
Cuts at: 1870 2343 5612 1870
Size: 473 3269 4545
Fragments arranged by size:
4545 3269 473
XmnI GAAnn'nnTTC
Cuts at: 2285 6416 2285
Size: 4131 4156
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5349 6095 276
Size: 53 83 186 936 3815 746 2468
Fragments arranged by size:
3815 2468 936 746 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BsiWI BstZ17I
Bsu36I DraIII FseI FspAI NruI PacI PmlI PpuMI
PspOMI RsrII SanDI SexAI SfiI SrfI SwaI