(Circular) (Six-base) MAPSORT of: pFN21ASDA1095.seq Check: 3473 from: 1 to: 7927 pFN21ASDA1095 7927 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3013 3851 3013 Size: 838 7089 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5737 278 Size: 53 83 186 936 4201 2468 Fragments arranged by size: 4201 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5190 5220 5270 1937 Size: 3253 30 50 4594 Fragments arranged by size: 4594 3253 50 30 Acc65I G'GTAC_C Cuts at: 5248 5248 Size: 7927 AclI AA'CG_TT Cuts at: 1563 6054 6427 1563 Size: 4491 373 3063 Fragments arranged by size: 4491 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 3865 4520 4886 5990 7374 7642 1632 Size: 458 1775 655 366 1104 1384 268 1917 Fragments arranged by size: 1917 1775 1384 1104 655 458 366 268 AfeI AGC'GCT Cuts at: 2333 2517 3956 2333 Size: 184 1439 6304 Fragments arranged by size: 6304 1439 184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7730 AflIII A'CryG_T Cuts at: 1203 2418 6826 7803 1203 Size: 1215 4408 977 1327 Fragments arranged by size: 4408 1327 1215 977 AgeI A'CCGG_T Cuts at: 6758 6758 Size: 7927 AhdI GACnn_n'nnGTC Cuts at: 6656 6656 Size: 7927 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7927 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4241 1184 Size: 3057 4870 AlwNI CAG_nnn'CTG Cuts at: 2314 2383 2718 4525 4585 4966 5670 7242 2314 Size: 69 335 1807 60 381 704 1572 2999 Fragments arranged by size: 2999 1807 1572 704 381 335 69 60 ApaLI G'TGCA_C Cuts at: 5984 7140 5984 Size: 1156 6771 ApoI r'AATT_y Cuts at: 1493 2678 2919 5236 5462 5614 7723 1493 Size: 1185 241 2317 226 152 2109 1697 Fragments arranged by size: 2317 2109 1697 1185 241 226 152 AseI AT'TA_AT Cuts at: 160 1032 3441 6481 160 Size: 872 2409 3040 1606 Fragments arranged by size: 3040 2409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7927 AvaI C'yCGr_G Cuts at: 1870 1943 2327 4285 4387 5252 1870 Size: 73 384 1958 102 865 4545 Fragments arranged by size: 4545 1958 865 384 102 73 BamHI G'GATC_C Cuts at: 3166 3933 5257 5624 3166 Size: 767 1324 367 5469 Fragments arranged by size: 5469 1324 767 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4475 5248 6703 618 Size: 325 217 627 2688 773 1455 1842 Fragments arranged by size: 2688 1842 1455 773 627 325 217 BanII G_rGCy'C Cuts at: 1391 2217 3012 3773 3969 3978 4242 4260 4568 Size: 826 795 761 196 9 264 18 308 Cuts at: 4568 4754 5246 1391 Size: 186 492 4072 Fragments arranged by size: 4072 826 795 761 492 308 264 196 186 18 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2122 3354 3678 928 Size: 971 223 1232 324 5177 Fragments arranged by size: 5177 1232 971 324 223 BbvCI CC'TCA_GC Cuts at: 3196 3196 Size: 7927 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6118 6152 1903 Size: 34 4181 34 3678 Fragments arranged by size: 4181 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2881 5819 7029 2881 Size: 2938 1210 3779 Fragments arranged by size: 3779 2938 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7927 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2454 2627 6538 Size: 107 122 71 631 605 782 173 3911 Cuts at: 6538 136 Size: 1525 Fragments arranged by size: 3911 1525 782 631 605 173 122 107 71 BglII A'GATC_T Cuts at: 4338 7922 4338 Size: 3584 4343 BlpI GC'TnA_GC Cuts at: 2711 5344 2711 Size: 2633 5294 Bme1580I G_kGCm'C Cuts at: 1790 4478 5988 7144 1790 Size: 2688 1510 1156 2573 Fragments arranged by size: 2688 2573 1510 1156 BmgBI CAC'GTC Cuts at: 1318 2056 2128 2224 2398 4225 1318 Size: 738 72 96 174 1827 5020 Fragments arranged by size: 5020 1827 738 174 96 72 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5658 6616 442 Size: 416 53 83 399 24 4241 958 1753 Fragments arranged by size: 4241 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3645 1056 Size: 2589 5338 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3211 3211 Size: 7927 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2525 3287 3293 3521 3626 3992 4097 Size: 878 408 762 6 228 105 366 105 Cuts at: 4097 4133 4355 4960 6587 1239 Size: 36 222 605 1627 2579 Fragments arranged by size: 2579 1627 878 762 605 408 366 228 222 105 105 36 6 Bpu10I CC'TnA_GC Cuts at: 3196 4121 4748 3196 Size: 925 627 6375 Fragments arranged by size: 6375 925 627 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2219 2234 2558 2663 3962 4139 4378 5015 6052 Size: 15 324 105 1299 177 239 637 1037 Cuts at: 6052 6917 7215 2219 Size: 865 298 2931 Fragments arranged by size: 2931 1299 1037 865 637 324 298 239 177 105 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3417 6590 882 Size: 617 1918 3173 2219 Fragments arranged by size: 3173 2219 1918 617 BsaAI yAC'GTr Cuts at: 493 1204 5224 5718 493 Size: 711 4020 494 2702 Fragments arranged by size: 4020 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3171 3365 5138 7921 3171 Size: 194 1773 2783 3177 Fragments arranged by size: 3177 2783 1773 194 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3798 3918 4038 5734 Size: 53 83 186 936 2265 120 120 1696 Cuts at: 5734 6116 6751 275 Size: 382 635 1451 Fragments arranged by size: 2265 1696 1451 936 635 382 186 120 120 83 53 BsaWI w'CCGG_w Cuts at: 3333 6360 6758 7032 7179 7590 3333 Size: 3027 398 274 147 411 3670 Fragments arranged by size: 3670 3027 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 3965 3995 4589 Size: 30 165 30 1374 30 1158 30 594 Cuts at: 4589 4619 1178 Size: 30 4486 Fragments arranged by size: 4486 1374 1158 594 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3200 3275 3623 4375 4964 1186 Size: 2014 75 348 752 589 4149 Fragments arranged by size: 4149 2014 752 589 348 75 BseYI C'CCAG_C Cuts at: 2594 2622 3149 3313 4574 4585 4653 7130 7626 Size: 28 527 164 1261 11 68 2477 496 Cuts at: 7626 2594 Size: 2895 Fragments arranged by size: 2895 2477 1261 527 496 164 68 28 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 2546 3041 3476 3575 3895 4169 Size: 573 447 363 495 435 99 320 274 Cuts at: 4169 1163 Size: 4921 Fragments arranged by size: 4921 573 495 447 435 363 320 274 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2197 2293 2825 3906 4698 5377 Size: 275 186 196 96 532 1081 792 679 Cuts at: 5377 6138 7166 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1081 1028 792 761 679 532 275 196 186 96 BsiHKAI G_wGCw'C Cuts at: 2217 2325 3755 3773 3969 3978 4242 4260 4754 Size: 108 1430 18 196 9 264 18 494 Cuts at: 4754 5988 6073 7144 2217 Size: 1234 85 1071 3000 Fragments arranged by size: 3000 1430 1234 1071 494 264 196 108 85 18 18 9 BsiWI C'GTAC_G Cuts at: 2271 2271 Size: 7927 BsmI GAATG_Cn' Cuts at: 5438 5531 5438 Size: 93 7834 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2325 3012 3755 3773 3969 3978 Size: 399 427 108 687 743 18 196 9 Cuts at: 3978 4242 4260 4478 4568 4754 5246 5988 6073 Size: 264 18 218 90 186 492 742 85 Cuts at: 6073 7144 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 743 742 687 492 427 399 264 218 196 186 108 90 85 18 18 9 BspEI T'CCGG_A Cuts at: 7590 7590 Size: 7927 BspHI T'CATG_A Cuts at: 2923 2971 5004 5816 7546 2923 Size: 48 2033 812 1730 3304 Fragments arranged by size: 3304 2033 1730 812 48 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3013 3597 3851 3882 4608 5007 5282 844 Size: 2169 584 254 31 726 399 275 3489 Fragments arranged by size: 3489 2169 726 584 399 275 254 31 BsrBI CCG'CTC Cuts at: 1394 1823 2233 4327 4367 4540 5814 1394 Size: 429 410 2094 40 173 1274 3507 Fragments arranged by size: 3507 2094 1274 429 410 173 40 BsrDI GCAATG_nn' Cuts at: 65 1248 3390 3512 6422 6596 65 Size: 1183 2142 122 2910 174 1396 Fragments arranged by size: 2910 2142 1396 1183 174 122 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 2820 4040 6571 6758 1700 Size: 110 328 682 1220 2531 187 2869 Fragments arranged by size: 2869 2531 1220 682 328 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7927 BssHII G'CGCG_C Cuts at: 1925 2076 2183 2351 2488 2508 2535 2556 2590 Size: 151 107 168 137 20 27 21 34 Cuts at: 2590 2694 2696 4132 4858 1925 Size: 104 2 1436 726 4994 Fragments arranged by size: 4994 1436 726 168 151 137 107 104 34 27 21 20 2 BssSI C'ACGA_G Cuts at: 3716 4235 5987 6999 3716 Size: 519 1752 1012 4644 Fragments arranged by size: 4644 1752 1012 519 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 4383 794 Size: 1520 2069 4338 Fragments arranged by size: 4338 2069 1520 BstBI TT'CG_AA Cuts at: 2007 6731 2007 Size: 4724 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7927 BstXI CCAn_nnnn'nTGG Cuts at: 3443 3820 3443 Size: 377 7550 BstYI r'GATC_y Cuts at: 1972 3166 3933 4338 4462 4794 4869 5257 5624 Size: 1194 767 405 124 332 75 388 367 Cuts at: 5624 6009 6026 7478 7564 7576 7922 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1194 767 405 388 385 367 346 332 124 86 75 17 12 BstZ17I GTA'TAC Cuts at: 5191 5221 5191 Size: 30 7897 BtgI C'CryG_G Cuts at: 513 1065 2022 2191 2631 4413 4698 5158 513 Size: 552 957 169 440 1782 285 460 3282 Fragments arranged by size: 3282 1782 957 552 460 440 285 169 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7188 Fragments arranged by size: 7188 683 56 BtsI GCAGTG_nn' Cuts at: 2500 5443 6237 6257 2500 Size: 2943 794 20 4170 Fragments arranged by size: 4170 2943 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7927 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2351 2386 529 Size: 35 1787 35 6070 Fragments arranged by size: 6070 1787 35 35 DraI TTT'AAA Cuts at: 5231 5231 Size: 7927 DraIII CAC_nnn'GTG Cuts at: 2118 3327 4969 2118 Size: 1209 1642 5076 Fragments arranged by size: 5076 1642 1209 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6934 809 Size: 507 5618 1802 Fragments arranged by size: 5618 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2205 2290 Size: 54 1425 50 275 18 292 83 85 Cuts at: 2290 2374 2432 2497 2628 2724 2818 2822 3903 Size: 84 58 65 131 96 94 4 1081 Cuts at: 3903 4695 5374 6263 7892 8 Size: 792 679 889 1629 43 Fragments arranged by size: 1629 1425 1081 889 792 679 292 275 131 96 94 85 84 83 65 58 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2290 2822 3903 4695 5374 1537 Size: 275 478 532 1081 792 679 4090 Fragments arranged by size: 4090 1081 792 679 532 478 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3462 3486 3986 4086 4872 5099 5857 1353 Size: 2109 24 500 100 786 227 758 3423 Fragments arranged by size: 3423 2109 786 758 500 227 100 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3754 4385 6514 6888 7034 387 Size: 1064 138 2165 631 2129 374 146 1280 Fragments arranged by size: 2165 2129 1280 1064 631 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 3771 3967 3976 4240 4258 4752 2215 Size: 1556 196 9 264 18 494 5390 Fragments arranged by size: 5390 1556 494 264 196 18 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2117 2525 3287 3293 3521 3626 Size: 393 458 27 408 762 6 228 105 Cuts at: 3626 3865 3992 4097 4133 4355 4520 4886 4960 Size: 239 127 105 36 222 165 366 74 Cuts at: 4960 5990 6587 7374 7642 1239 Size: 1030 597 787 268 1524 Fragments arranged by size: 1524 1030 787 762 597 458 408 393 366 268 239 228 222 165 127 105 105 74 36 27 6 EcoNI CCTnn'n_nnAGG Cuts at: 4970 4970 Size: 7927 EcoO109I rG'GnC_Cy Cuts at: 2092 2101 4744 2092 Size: 9 2643 5275 Fragments arranged by size: 5275 2643 9 EcoRI G'AATT_C Cuts at: 5236 5614 5236 Size: 378 7549 EcoRV GAT'ATC Cuts at: 4249 4249 Size: 7927 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5137 5137 Size: 7927 FseI GG_CCGG'CC Cuts at: 2824 2824 Size: 7927 FspI TGC'GCA Cuts at: 4100 4229 6433 4100 Size: 129 2204 5594 Fragments arranged by size: 5594 2204 129 HaeII r_GCGC'y Cuts at: 1678 2242 2335 2519 2573 2588 3958 3999 4293 Size: 564 93 184 54 15 1370 41 294 Cuts at: 4293 4596 4936 7074 1678 Size: 303 340 2138 2531 Fragments arranged by size: 2531 2138 1370 564 340 303 294 184 93 54 41 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2777 2809 2834 2866 3130 Size: 32 135 32 1205 32 25 32 264 Cuts at: 3130 3162 3755 3787 4224 4256 4736 4768 6568 Size: 32 593 32 437 32 480 32 1800 Cuts at: 6568 6600 6642 6674 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1800 1205 593 480 437 264 135 42 32 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 2884 2944 5271 5517 669 Size: 988 281 946 60 2327 246 3079 Fragments arranged by size: 3079 2327 988 946 281 246 60 HindIII A'AGCT_T Cuts at: 748 2988 748 Size: 2240 5687 HpaI GTT'AAC Cuts at: 5517 5517 Size: 7927 KpnI G_GTAC'C Cuts at: 5252 5252 Size: 7927 MfeI C'AATT_G Cuts at: 5526 5526 Size: 7927 MluI A'CGCG_T Cuts at: 2418 7803 2418 Size: 5385 2542 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3732 3837 4296 4746 5001 7041 7225 1305 Size: 2427 105 459 450 255 2040 184 2007 Fragments arranged by size: 2427 2040 2007 459 450 255 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6105 Fragments arranged by size: 6105 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 3137 3599 4223 5885 6244 6403 Size: 648 1851 120 462 624 1662 359 159 Cuts at: 6403 518 Size: 2042 Fragments arranged by size: 2042 1851 1662 648 624 462 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2107 2175 2193 2314 2445 2626 2633 2718 Size: 501 68 18 121 131 181 7 85 Cuts at: 2718 3079 3541 3729 3820 3867 4194 4383 4678 Size: 361 462 188 91 47 327 189 295 Cuts at: 4678 4887 4987 5343 6020 7168 7413 1606 Size: 209 100 356 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 501 462 361 356 327 295 245 209 189 188 181 131 121 100 91 85 68 47 18 7 NaeI GCC'GGC Cuts at: 2140 2822 4042 2140 Size: 682 1220 6025 Fragments arranged by size: 6025 1220 682 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6418 Fragments arranged by size: 6418 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7927 NgoMIV G'CCGG_C Cuts at: 2138 2820 4040 2138 Size: 682 1220 6025 Fragments arranged by size: 6025 1220 682 NheI G'CTAG_C Cuts at: 1052 3641 1052 Size: 2589 5338 NotI GC'GGCC_GC Cuts at: 5374 5374 Size: 7927 NspI r_CATG'y Cuts at: 1132 1229 2413 3604 4675 4738 5285 6830 1132 Size: 97 1184 1191 1071 63 547 1545 2229 Fragments arranged by size: 2229 1545 1191 1184 1071 547 97 63 PasI CC'CwG_GG Cuts at: 1348 2154 2797 1348 Size: 806 643 6478 Fragments arranged by size: 6478 806 643 PciI A'CATG_T Cuts at: 6826 6826 Size: 7927 PflMI CCAn_nnn'nTGG Cuts at: 1108 2983 4525 4585 1108 Size: 1875 1542 60 4450 Fragments arranged by size: 4450 1875 1542 60 PfoI T'CCnGG_A Cuts at: 2094 3969 4175 4260 4872 2094 Size: 1875 206 85 612 5149 Fragments arranged by size: 5149 1875 612 206 85 PmeI GTTT'AAAC Cuts at: 5231 5231 Size: 7927 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5996 6028 7509 7541 5996 Size: 32 1481 32 6382 Fragments arranged by size: 6382 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2101 4744 2092 Size: 9 2643 5275 Fragments arranged by size: 5275 2643 9 PshAI GACnn'nnGTC Cuts at: 1613 2771 1613 Size: 1158 6769 PsiI TTA'TAA Cuts at: 5497 5497 Size: 7927 PspXI vC'TCGA_Gb Cuts at: 2327 4285 2327 Size: 1958 5969 PstI C_TGCA'G Cuts at: 830 2310 2652 2899 3654 3848 5279 830 Size: 1480 342 247 755 194 1431 3478 Fragments arranged by size: 3478 1480 1431 755 342 247 194 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7927 PvuII CAG'CTG Cuts at: 2175 2314 2626 3079 3541 4383 4678 2175 Size: 139 312 453 462 842 295 5424 Fragments arranged by size: 5424 842 462 453 312 295 139 RsrII CG'GwC_CG Cuts at: 3741 3741 Size: 7927 SacI G_AGCT'C Cuts at: 2217 3773 3969 3978 4242 4260 4754 2217 Size: 1556 196 9 264 18 494 5390 Fragments arranged by size: 5390 1556 494 264 196 18 9 SacII CC_GC'GG Cuts at: 2194 2634 2194 Size: 440 7487 SalI G'TCGA_C Cuts at: 1936 5269 1936 Size: 3333 4594 SapI GCTCTTCn'nnn_ Cuts at: 3486 3986 4086 4872 3486 Size: 500 100 786 6541 Fragments arranged by size: 6541 786 500 100 SbfI CC_TGCA'GG Cuts at: 5279 5279 Size: 7927 ScaI AGT'ACT Cuts at: 6175 7887 6175 Size: 1712 6215 SexAI A'CCwGG_T Cuts at: 4965 4965 Size: 7927 SfcI C'TryA_G Cuts at: 826 1045 2306 2648 2895 3289 3650 3844 4486 Size: 219 1261 342 247 394 361 194 642 Cuts at: 4486 5275 6410 7091 7282 826 Size: 789 1135 681 191 1471 Fragments arranged by size: 1471 1261 1135 789 681 642 394 361 342 247 219 194 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2627 2627 Size: 7927 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7927 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7927 SmaI CCC'GGG Cuts at: 1872 5254 1872 Size: 3382 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 2249 2327 2573 2642 3977 Size: 197 926 291 15 78 246 69 1335 Cuts at: 3977 4154 4285 4357 5030 6031 6932 7194 820 Size: 177 131 72 673 1001 901 262 1553 Fragments arranged by size: 1553 1335 1001 926 901 673 291 262 246 197 177 131 78 72 69 15 SnaBI TAC'GTA Cuts at: 493 5224 5718 493 Size: 4731 494 2702 Fragments arranged by size: 4731 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7927 SphI G_CATG'C Cuts at: 1132 5285 1132 Size: 4153 3774 SspI AAT'ATT Cuts at: 5 52 2809 5851 5 Size: 47 2757 3042 2081 Fragments arranged by size: 3042 2757 2081 47 StuI AGG'CCT Cuts at: 1517 2913 3529 1517 Size: 1396 616 5915 Fragments arranged by size: 5915 1396 616 StyI C'CwwG_G Cuts at: 513 1065 2022 2901 3003 3041 3399 3858 4977 Size: 552 957 879 102 38 358 459 1119 Cuts at: 4977 5366 513 Size: 389 3074 Fragments arranged by size: 3074 1119 957 879 552 459 389 358 102 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2146 3758 5954 5971 6124 6309 6754 Size: 1255 566 1612 2196 17 153 185 445 Cuts at: 6754 325 Size: 1498 Fragments arranged by size: 2196 1612 1498 1255 566 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4014 6173 7885 Size: 274 80 33 51 1443 2037 2159 1712 Cuts at: 7885 96 Size: 138 Fragments arranged by size: 2159 2037 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 2249 2281 3965 3997 4447 4479 7887 Size: 32 160 32 1684 32 450 32 3408 Cuts at: 7887 7919 2057 Size: 32 2065 Fragments arranged by size: 3408 2065 1684 450 160 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7927 XbaI T'CTAG_A Cuts at: 5263 5263 Size: 7927 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4522 4582 4522 Size: 60 7867 XhoI C'TCGA_G Cuts at: 1943 2327 4285 1943 Size: 384 1958 5585 Fragments arranged by size: 5585 1958 384 XmaI C'CCGG_G Cuts at: 1870 5252 1870 Size: 3382 4545 XmnI GAAnn'nnTTC Cuts at: 2678 5120 6056 2678 Size: 2442 936 4549 Fragments arranged by size: 4549 2442 936 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5735 276 Size: 53 83 186 936 4201 2468 Fragments arranged by size: 4201 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsmBI Bsu36I FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI SanDI SfoI SrfI SwaI