Restriction map for
pFN21ASDA1094
(Circular) (Six-base) MAPSORT of: pFN21ASDA1094.seq Check: 6558 from: 1 to: 6343
pFN21ASDA1094 6343 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4153 278
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3686 1937
Size: 1749 4594
Acc65I G'GTAC_C
Cuts at: 3664 3664
Size: 6343
AclI AA'CG_TT
Cuts at: 1563 4470 4843 1563
Size: 2907 373 3063
Fragments arranged by size:
3063 2907 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2728 2870 4406 5790 6058 1632
Size: 1096 142 1536 1384 268 1917
Fragments arranged by size:
1917 1536 1384 1096 268 142
AfeI AGC'GCT
Cuts at: 2654 2654
Size: 6343
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6146
AflIII A'CryG_T
Cuts at: 1203 5242 6219 1203
Size: 4039 977 1327
Fragments arranged by size:
4039 1327 977
AgeI A'CCGG_T
Cuts at: 5174 5174
Size: 6343
AhdI GACnn_n'nnGTC
Cuts at: 5072 5072
Size: 6343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6343
AlwNI CAG_nnn'CTG
Cuts at: 2372 3297 4086 5658 2372
Size: 925 789 1572 3057
Fragments arranged by size:
3057 1572 925 789
ApaI G_GGCC'C
Cuts at: 3334 3334
Size: 6343
ApaLI G'TGCA_C
Cuts at: 4400 5556 4400
Size: 1156 5187
ApoI r'AATT_y
Cuts at: 1493 3652 3878 4030 6139 1493
Size: 2159 226 152 2109 1697
Fragments arranged by size:
2159 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4897 160
Size: 872 3865 1606
Fragments arranged by size:
3865 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2050 3668 1870
Size: 73 85 22 1618 4545
Fragments arranged by size:
4545 1618 85 73 22
BamHI G'GATC_C
Cuts at: 3673 4040 3673
Size: 367 5976
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2316 2774 3664 5119 618
Size: 325 217 627 529 458 890 1455 1842
Fragments arranged by size:
1842 1455 890 627 529 458 325 217
BanII G_rGCy'C
Cuts at: 1391 2065 3334 3662 1391
Size: 674 1269 328 4072
Fragments arranged by size:
4072 1269 674 328
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 2752 3121 928
Size: 971 325 528 369 4150
Fragments arranged by size:
4150 971 528 369 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4534 4568 1903
Size: 34 2597 34 3678
Fragments arranged by size:
3678 2597 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4235 5445 4235
Size: 1210 5133
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6343
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2951 4954 136
Size: 107 122 71 631 605 1279 2003 1525
Fragments arranged by size:
2003 1525 1279 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6338 6338
Size: 6343
BlpI GC'TnA_GC
Cuts at: 2117 2819 3760 2117
Size: 702 941 4700
Fragments arranged by size:
4700 941 702
Bme1580I G_kGCm'C
Cuts at: 1790 2039 3334 4404 5560 1790
Size: 249 1295 1070 1156 2573
Fragments arranged by size:
2573 1295 1156 1070 249
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 2681 4074
Size: 416 53 83 399 24 1003 261 1393
Cuts at: 4074 5032 442
Size: 958 1753
Fragments arranged by size:
1753 1393 1003 958 416 399 261 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 3161 5003 1239
Size: 1245 677 1842 2579
Fragments arranged by size:
2579 1842 1245 677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4468 5333 5631 4468
Size: 865 298 5180
Fragments arranged by size:
5180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2940 5006 882
Size: 617 1441 2066 2219
Fragments arranged by size:
2219 2066 1441 617
BsaAI yAC'GTr
Cuts at: 493 1204 3640 4134 493
Size: 711 2436 494 2702
Fragments arranged by size:
2702 2436 711 494
BsaBI GATnn'nnATC
Cuts at: 3494 6337 3494
Size: 2843 3500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4150 4532 5167 275
Size: 53 83 186 936 2617 382 635 1451
Fragments arranged by size:
2617 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4776 5174 5448 5595 6006 4776
Size: 398 274 147 411 5113
Fragments arranged by size:
5113 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6118
Fragments arranged by size:
6118 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2776 3336 1186
Size: 1590 560 4193
Fragments arranged by size:
4193 1590 560
BseYI C'CCAG_C
Cuts at: 2327 5546 6042 2327
Size: 3219 496 2628
Fragments arranged by size:
3219 2628 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2831 3283 1163
Size: 573 459 636 452 4223
Fragments arranged by size:
4223 636 573 459 452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3793 4554 5582 1540
Size: 275 186 1792 761 1028 2301
Fragments arranged by size:
2301 1792 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4404 4489 5560 4404
Size: 85 1071 5187
Fragments arranged by size:
5187 1071 85
BsmI GAATG_Cn'
Cuts at: 2983 3854 3947 2983
Size: 871 93 5379
Fragments arranged by size:
5379 871 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2065 3334 3662 4404 4489 5560
Size: 399 249 26 1269 328 742 85 1071
Cuts at: 5560 1391
Size: 2174
Fragments arranged by size:
2174 1269 1071 742 399 328 249 85
26
BspEI T'CCGG_A
Cuts at: 6006 6006
Size: 6343
BspHI T'CATG_A
Cuts at: 2304 2484 2805 4232 5962 2304
Size: 180 321 1427 1730 2685
Fragments arranged by size:
2685 1730 1427 321 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2580 3261 3698 844
Size: 1736 681 437 3489
Fragments arranged by size:
3489 1736 681 437
BsrBI CCG'CTC
Cuts at: 1394 1823 2314 4230 1394
Size: 429 491 1916 3507
Fragments arranged by size:
3507 1916 491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4838 5012 65
Size: 1183 3590 174 1396
Fragments arranged by size:
3590 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 2850 3087 3347 4987 5174 1700
Size: 110 395 645 237 260 1640 187 2869
Fragments arranged by size:
2869 1640 645 395 260 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6343
BssSI C'ACGA_G
Cuts at: 2149 4403 5415 2149
Size: 2254 1012 3077
Fragments arranged by size:
3077 2254 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6343
BstBI TT'CG_AA
Cuts at: 2007 5147 2007
Size: 3140 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6343
BstXI CCAn_nnnn'nTGG
Cuts at: 2327 2377 2327
Size: 50 6293
BstYI r'GATC_y
Cuts at: 1972 3411 3673 4040 4425 4442 5894 5980 5992
Size: 1439 262 367 385 17 1452 86 12
Cuts at: 5992 6338 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1439 385 367 346 262 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2160 2277 3402 513
Size: 552 1095 117 1125 3454
Fragments arranged by size:
3454 1125 1095 552 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3010 487
Size: 683 56 1784 3820
Fragments arranged by size:
3820 1784 683 56
BtsI GCAGTG_nn'
Cuts at: 2115 2178 2388 2603 3092 3859 4653 4673 2115
Size: 63 210 215 489 767 794 20 3785
Fragments arranged by size:
3785 794 767 489 215 210 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2180 2215 529
Size: 35 1616 35 4657
Fragments arranged by size:
4657 1616 35 35
DraI TTT'AAA
Cuts at: 3647 3647
Size: 6343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5350 809
Size: 507 4034 1802
Fragments arranged by size:
4034 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2943 3089 3790
Size: 54 1425 50 275 18 1113 146 701
Cuts at: 3790 4679 6308 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1113 889 701 275 146 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3790 1537
Size: 275 1978 4090
Fragments arranged by size:
4090 1978 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2396 2900 3276 4273 1353
Size: 1043 504 376 997 3423
Fragments arranged by size:
3423 1043 997 504 376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4930 5304 5450 387
Size: 1064 138 3341 374 146 1280
Fragments arranged by size:
3341 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 2728 2870 3161 4406 5003 5790
Size: 393 852 244 142 291 1245 597 787
Cuts at: 5790 6058 1239
Size: 268 1524
Fragments arranged by size:
1524 1245 852 787 597 393 291 268
244 142
EcoO109I rG'GnC_Cy
Cuts at: 2054 2683 3016 3330 3331 2054
Size: 629 333 314 1 5066
Fragments arranged by size:
5066 629 333 314 1
EcoRI G'AATT_C
Cuts at: 3652 4030 3652
Size: 378 5965
FspI TGC'GCA
Cuts at: 3468 4849 3468
Size: 1381 4962
HaeII r_GCGC'y
Cuts at: 1678 2229 2411 2656 5490 1678
Size: 551 182 245 2834 2531
Fragments arranged by size:
2834 2531 551 245 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2132 2164 2457
Size: 32 135 32 452 32 76 32 293
Cuts at: 2457 2489 3580 3612 4984 5016 5058 5090 1373
Size: 32 1091 32 1372 32 42 32 2626
Fragments arranged by size:
2626 1372 1091 452 293 135 76 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3687 3933 669
Size: 988 281 1749 246 3079
Fragments arranged by size:
3079 1749 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6343
HpaI GTT'AAC
Cuts at: 3933 3933
Size: 6343
KpnI G_GTAC'C
Cuts at: 3668 3668
Size: 6343
MfeI C'AATT_G
Cuts at: 3942 3942
Size: 6343
MluI A'CGCG_T
Cuts at: 6219 6219
Size: 6343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5457 5641 1305
Size: 4152 184 2007
Fragments arranged by size:
4152 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2945 10
Size: 54 1425 343 1113 3408
Fragments arranged by size:
3408 1425 1113 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 2360 2375 2381 4301 4660 4819
Size: 648 1116 78 15 6 1920 359 159
Cuts at: 4819 518
Size: 2042
Fragments arranged by size:
2042 1920 1116 648 359 159 78 15
6
MspA1I CmG'CkG
Cuts at: 1606 2068 2583 3417 3759 4436 5584 5829 1606
Size: 462 515 834 342 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 677 515 462 342 245
NcoI C'CATG_G
Cuts at: 513 1065 2277 3402 513
Size: 552 1212 1125 3454
Fragments arranged by size:
3454 1212 1125 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6343
NotI GC'GGCC_GC
Cuts at: 3790 3790
Size: 6343
NspI r_CATG'y
Cuts at: 1132 1229 3268 3701 5246 1132
Size: 97 2039 433 1545 2229
Fragments arranged by size:
2229 2039 1545 433 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6343
PciI A'CATG_T
Cuts at: 5242 5242
Size: 6343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6343
PfoI T'CCnGG_A
Cuts at: 2523 3138 3567 2523
Size: 615 429 5299
Fragments arranged by size:
5299 615 429
PmeI GTTT'AAAC
Cuts at: 3647 3647
Size: 6343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 5925 5957 4412
Size: 32 1481 32 4798
Fragments arranged by size:
4798 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2683 2683
Size: 6343
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6343
PsiI TTA'TAA
Cuts at: 3913 3913
Size: 6343
PspOMI G'GGCC_C
Cuts at: 3330 3330
Size: 6343
PstI C_TGCA'G
Cuts at: 830 3695 830
Size: 2865 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6343
SalI G'TCGA_C
Cuts at: 1936 3685 1936
Size: 1749 4594
SanDI GG'GwC_CC
Cuts at: 2683 2683
Size: 6343
SbfI CC_TGCA'GG
Cuts at: 3695 3695
Size: 6343
ScaI AGT'ACT
Cuts at: 4591 6303 4591
Size: 1712 4631
SfcI C'TryA_G
Cuts at: 826 1045 2213 3480 3523 3691 4826 5507 5698
Size: 219 1168 1267 43 168 1135 681 191
Cuts at: 5698 826
Size: 1471
Fragments arranged by size:
1471 1267 1168 1135 681 219 191 168
43
SfiI GGCCn_nnn'nGGCC
Cuts at: 2951 2951
Size: 6343
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6343
SmaI CCC'GGG
Cuts at: 1872 2052 3670 1872
Size: 180 1618 4545
Fragments arranged by size:
4545 1618 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4447 5348 5610 820
Size: 197 926 2504 901 262 1553
Fragments arranged by size:
2504 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3640 4134 493
Size: 3147 494 2702
Fragments arranged by size:
3147 2702 494
SpeI A'CTAG_T
Cuts at: 152 2299 152
Size: 2147 4196
SphI G_CATG'C
Cuts at: 1132 3701 1132
Size: 2569 3774
SspI AAT'ATT
Cuts at: 5 52 4267 5
Size: 47 4215 2081
Fragments arranged by size:
4215 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6343
StyI C'CwwG_G
Cuts at: 513 1065 2277 2824 2949 3402 3782 513
Size: 552 1212 547 125 453 380 3074
Fragments arranged by size:
3074 1212 552 547 453 380 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2157 2606 3353 3359 4370 4387 4540
Size: 1255 577 449 747 6 1011 17 153
Cuts at: 4540 4725 5170 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1011 747 577 449 445 185
153 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4589 6301 96
Size: 274 80 33 51 1443 2612 1712 138
Fragments arranged by size:
2612 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6303 6335 6303
Size: 32 6311
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6343
XbaI T'CTAG_A
Cuts at: 3679 3679
Size: 6343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3118 3118
Size: 6343
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6343
XmaI C'CCGG_G
Cuts at: 1870 2050 3668 1870
Size: 180 1618 4545
Fragments arranged by size:
4545 1618 180
XmnI GAAnn'nnTTC
Cuts at: 2336 2532 4472 2336
Size: 196 1940 4207
Fragments arranged by size:
4207 1940 196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4151 276
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfoI SrfI SwaI