Restriction map for
pFN21ASDA1093
##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BclI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CpoI Eam1105I EcoNI EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3717 GGTACC G^GTACC
AccII 278 CGCG CG^CG
AcyI 2204 GACGCC G[GA]^CG[CT]C
AflII 1146 CTTAAG C^TTAAG
AflIII 4778 ACATGT A^C[GA][CT]GT
Asp718I 3717 GGTACC G^GTACC
BciVI 4130 GTATCC GTATCC(6/5)
BfrI 1146 CTTAAG C^TTAAG
BglI 161 GCCCAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1678 AGATCT A^GATCT
BsaHI 2204 GACGCC G[GA]^CG[CT]C
BsmBI 2422 GAGACG CGTCTC(1/5)
BsmI 1178 GAATGC GAATGC(1/-1)
BspDI 3182 ATCGAT AT^CGAT
BspHI 3170 TCATGA T^CATGA
BspLU11I 4778 ACATGT A^CATGT
BsrFI 4918 ACCGGC [GA]^CCGG[CT]
BstUI 278 CGCG CG^CG
Cfr10I 4918 ACCGGC [GA]^CCGG[CT]
ClaI 3182 ATCGAT AT^CGAT
DraI 3853 TTTAAA TTT^AAA
EagI 152 CGGCCG C^GGCCG
EclXI 152 CGGCCG C^GGCCG
Eco52I 152 CGGCCG C^GGCCG
HgaI 2204 GACGC GACGC(5/10)
Hin1I 2204 GACGCC G[GA]^CG[CT]C
HincII 4650 GTCAAC GT[CT]^[GA]AC
HindII 4650 GTCAAC GT[CT]^[GA]AC
KpnI 3717 GGTACC GGTAC^C
MvnI 278 CGCG CG^CG
PaeR7I 2759 CTCGAG C^TCGAG
RcaI 3170 TCATGA T^CATGA
SmaI 3950 CCCGGG CCC^GGG
SpeI 706 ACTAGT A^CTAGT
StuI 1921 AGGCCT AGG^CCT
XhoI 2759 CTCGAG C^TCGAG
XmaCI 3950 CCCGGG C^CCGGG
XmaI 3950 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 574 GTAGAC GT^[AC][GT]AC
1434 GTCTAC
AclI 2548 AACGTT AA^CGTT
4327 AACGTT
Alw44I 998 GTGCAC G^TGCAC
3623 GTGCAC
ApaLI 998 GTGCAC G^TGCAC
3623 GTGCAC
AspI 1303 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1542 GACTGGGTC
BalI 931 TGGCCA TGG^CCA
4005 TGGCCA
BbsI 40 GAAGAC GAAGAC(2/6)
2226 GTCTTC
BlpI 3474 GCTGAGC GC^T[ACGT]AGC
4855 GCTCAGC
Bpu1102I 3474 GCTGAGC GC^T[ACGT]AGC
4855 GCTCAGC
BpuAI 40 GAAGAC GAAGAC(2/6)
2226 GTCTTC
BsiEI 152 CGGCCG CG[GA][CT]^CG
1771 CGGTCG
CelII 3474 GCTGAGC GC^T[ACGT]AGC
4855 GCTCAGC
DrdI 2842 GACGGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4156 GACCCCTATGTC
EcoRI 2736 GAATTC G^AATTC
4102 GAATTC
EcoRV 3166 GATATC GAT^ATC
3979 GATATC
MfeI 164 CAATTG C^AATTG
2277 CAATTG
MluNI 931 TGGCCA TGG^CCA
4005 TGGCCA
MscI 931 TGGCCA TGG^CCA
4005 TGGCCA
MunI 164 CAATTG C^AATTG
2277 CAATTG
NcoI 1163 CCATGG C^CATGG
4120 CCATGG
PflFI 1303 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1542 GACTGGGTC
Psp1406I 2548 AACGTT AA^CGTT
4327 AACGTT
SacI 2044 GAGCTC GAGCT^C
2858 GAGCTC
SapI 681 GAAGAGC GCTCTTC(1/4)
2626 GAAGAGC
SexAI 4284 ACCTGGT A^CC[AT]GGT
5130 ACCTGGT
SphI 344 GCATGC GCATG^C
4670 GCATGC
Tth111I 1303 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1542 GACTGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI Aor51HI ApaI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbvI BcgI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI