(Circular) (Six-base) MAPSORT of: pFN21ASDA1093.seq Check: 443 from: 1 to: 9895 pFN21ASDA1093 9895 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6901 6901 Size: 9895 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7705 278 Size: 53 83 186 936 6169 2468 Fragments arranged by size: 6169 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2598 3458 7238 1937 Size: 661 860 3780 4594 Fragments arranged by size: 4594 3780 860 661 Acc65I G'GTAC_C Cuts at: 5740 7216 5740 Size: 1476 8419 AclI AA'CG_TT Cuts at: 1563 4572 6351 8022 8395 1563 Size: 3009 1779 1671 373 3063 Fragments arranged by size: 3063 3009 1779 1671 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2902 4686 5109 5797 6348 7958 9342 9610 Size: 1270 1784 423 688 551 1610 1384 268 Cuts at: 9610 1632 Size: 1917 Fragments arranged by size: 1917 1784 1610 1384 1270 688 551 423 268 AfeI AGC'GCT Cuts at: 2448 3988 7024 2448 Size: 1540 3036 5319 Fragments arranged by size: 5319 3036 1540 AflII C'TTAA_G Cuts at: 820 1017 3169 820 Size: 197 2152 7546 Fragments arranged by size: 7546 2152 197 AflIII A'CryG_T Cuts at: 1203 6801 8794 9771 1203 Size: 5598 1993 977 1327 Fragments arranged by size: 5598 1993 1327 977 AgeI A'CCGG_T Cuts at: 8726 8726 Size: 9895 AhdI GACnn_n'nnGTC Cuts at: 8624 8624 Size: 9895 AleI CACnn'nnGTG Cuts at: 1166 5333 6804 1166 Size: 4167 1471 4257 Fragments arranged by size: 4257 4167 1471 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9895 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3390 3422 3390 Size: 32 9863 AlwNI CAG_nnn'CTG Cuts at: 2411 3335 5629 6934 7638 9210 2411 Size: 924 2294 1305 704 1572 3096 Fragments arranged by size: 3096 2294 1572 1305 924 704 ApaI G_GGCC'C Cuts at: 4775 5469 5718 7074 4775 Size: 694 249 1356 7596 Fragments arranged by size: 7596 1356 694 249 ApaLI G'TGCA_C Cuts at: 3021 5646 7952 9108 3021 Size: 2625 2306 1156 3808 Fragments arranged by size: 3808 2625 2306 1156 ApoI r'AATT_y Cuts at: 1493 2518 3120 3616 4759 5144 5347 5514 6125 Size: 1025 602 496 1143 385 203 167 611 Cuts at: 6125 7204 7430 7582 9691 1493 Size: 1079 226 152 2109 1697 Fragments arranged by size: 2109 1697 1143 1079 1025 611 602 496 385 226 203 167 152 AseI AT'TA_AT Cuts at: 160 1032 8449 160 Size: 872 7417 1606 Fragments arranged by size: 7417 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9895 AvaI C'yCGr_G Cuts at: 1870 1943 2286 2533 4782 5174 5973 7004 7220 Size: 73 343 247 2249 392 799 1031 216 Cuts at: 7220 1870 Size: 4545 Fragments arranged by size: 4545 2249 1031 799 392 343 247 216 73 BamHI G'GATC_C Cuts at: 7225 7592 7225 Size: 367 9528 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2293 2555 3937 5740 6311 Size: 325 217 627 506 262 1382 1803 571 Cuts at: 6311 7216 8671 618 Size: 905 1455 1842 Fragments arranged by size: 1842 1803 1455 1382 905 627 571 506 325 262 217 BanII G_rGCy'C Cuts at: 1391 2534 4071 4775 4885 5469 5718 5766 7005 Size: 1143 1537 704 110 584 249 48 1239 Cuts at: 7005 7074 7214 1391 Size: 69 140 4072 Fragments arranged by size: 4072 1537 1239 1143 704 584 249 140 110 69 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 4242 928 Size: 971 171 2172 6581 Fragments arranged by size: 6581 2172 971 171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2292 2326 5305 5339 5615 5649 6676 Size: 34 355 34 2979 34 276 34 1027 Cuts at: 6676 6710 8086 8120 1903 Size: 34 1376 34 3678 Fragments arranged by size: 3678 2979 1376 1027 355 276 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6164 7787 8997 6164 Size: 1623 1210 7062 Fragments arranged by size: 7062 1623 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9895 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2190 8506 136 Size: 107 122 71 631 605 518 6316 1525 Fragments arranged by size: 6316 1525 631 605 518 122 107 71 BglII A'GATC_T Cuts at: 3701 9890 3701 Size: 6189 3706 BlpI GC'TnA_GC Cuts at: 5498 6879 7312 5498 Size: 1381 433 8081 Fragments arranged by size: 8081 1381 433 Bme1580I G_kGCm'C Cuts at: 1790 2142 3025 4775 5469 5650 5718 6743 7074 Size: 352 883 1750 694 181 68 1025 331 Cuts at: 7074 7956 9112 1790 Size: 882 1156 2573 Fragments arranged by size: 2573 1750 1156 1025 883 882 694 352 331 181 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9895 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3288 3429 3576 Size: 416 53 83 399 24 1871 141 147 Cuts at: 3576 4156 4632 5352 5377 5464 5515 6032 7008 Size: 580 476 720 25 87 51 517 976 Cuts at: 7008 7626 8584 442 Size: 618 958 1753 Fragments arranged by size: 1871 1753 976 958 720 618 580 517 476 416 399 147 141 87 83 53 51 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9895 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2433 3217 3285 3648 4548 4615 6105 6448 Size: 1194 784 68 363 900 67 1490 343 Cuts at: 6448 6550 6622 6956 8555 1239 Size: 102 72 334 1599 2579 Fragments arranged by size: 2579 1599 1490 1194 900 784 363 343 334 102 72 68 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5402 5583 6700 8020 8885 9183 5402 Size: 181 1117 1320 865 298 6114 Fragments arranged by size: 6114 1320 1117 865 298 181 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3544 4707 6932 8558 882 Size: 617 2045 1163 2225 1626 2219 Fragments arranged by size: 2225 2219 2045 1626 1163 617 BsaAI yAC'GTr Cuts at: 493 1204 7192 7686 493 Size: 711 5988 494 2702 Fragments arranged by size: 5988 2702 711 494 BsaBI GATnn'nnATC Cuts at: 9889 9889 Size: 9895 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4228 7702 8084 8719 Size: 53 83 186 936 2695 3474 382 635 Cuts at: 8719 275 Size: 1451 Fragments arranged by size: 3474 2695 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8328 8726 9000 9147 9558 8328 Size: 398 274 147 411 8665 Fragments arranged by size: 8665 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 3143 3173 3390 Size: 30 165 30 1140 30 570 30 217 Cuts at: 3390 3420 6595 6625 1178 Size: 30 3175 30 4448 Fragments arranged by size: 4448 3175 1140 570 217 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2385 2533 4167 4919 4922 4932 5469 7185 Size: 1199 148 1634 752 3 10 537 1716 Cuts at: 7185 1186 Size: 3896 Fragments arranged by size: 3896 1716 1634 1199 752 537 148 10 3 BseYI C'CCAG_C Cuts at: 3888 4017 4031 4199 4432 4774 4851 4892 4896 Size: 129 14 168 233 342 77 41 4 Cuts at: 4896 4974 6205 9098 9594 3888 Size: 78 1231 2893 496 4189 Fragments arranged by size: 4189 2893 1231 496 342 233 168 129 78 77 41 14 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2386 3321 5309 5357 6777 6782 1163 Size: 573 650 935 1988 48 1420 5 4276 Fragments arranged by size: 4276 1988 1420 935 650 573 48 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2178 3797 7345 8106 9134 1540 Size: 275 186 177 1619 3548 761 1028 2301 Fragments arranged by size: 3548 2301 1619 1028 761 275 186 177 BsiHKAI G_wGCw'C Cuts at: 2711 3025 3242 3876 4071 4885 5009 5650 7956 Size: 314 217 634 195 814 124 641 2306 Cuts at: 7956 8041 9112 2711 Size: 85 1071 3494 Fragments arranged by size: 3494 2306 1071 814 641 634 314 217 195 124 85 BsmI GAATG_Cn' Cuts at: 3207 7406 7499 3207 Size: 4199 93 5603 Fragments arranged by size: 5603 4199 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4439 4439 Size: 9895 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2534 2711 3025 3242 3876 4071 Size: 399 352 392 177 314 217 634 195 Cuts at: 4071 4775 4885 5009 5469 5650 5718 5766 6743 Size: 704 110 124 460 181 68 48 977 Cuts at: 6743 7005 7074 7214 7956 8041 9112 1391 Size: 262 69 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 977 742 704 634 460 399 392 352 314 262 217 195 181 177 140 124 110 85 69 68 48 BspEI T'CCGG_A Cuts at: 9558 9558 Size: 9895 BspHI T'CATG_A Cuts at: 5193 7784 9514 5193 Size: 2591 1730 5574 Fragments arranged by size: 5574 2591 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4803 6070 6901 6979 7250 844 Size: 3959 1267 831 78 271 3489 Fragments arranged by size: 3959 3489 1267 831 271 78 BsrBI CCG'CTC Cuts at: 1394 1823 7782 1394 Size: 429 5959 3507 Fragments arranged by size: 5959 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3682 4390 6112 8390 8564 65 Size: 1183 2434 708 1722 2278 174 1396 Fragments arranged by size: 2434 2278 1722 1396 1183 708 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6941 8539 8726 1700 Size: 110 5131 1598 187 2869 Fragments arranged by size: 5131 2869 1598 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9895 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9895 BssSI C'ACGA_G Cuts at: 7955 8967 7955 Size: 1012 8883 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9895 BstBI TT'CG_AA Cuts at: 2007 8699 2007 Size: 6692 3203 BstEII G'GTnAC_C Cuts at: 1184 5982 6057 7157 1184 Size: 4798 75 1100 3922 Fragments arranged by size: 4798 3922 1100 75 BstXI CCAn_nnnn'nTGG Cuts at: 2239 2952 5239 2239 Size: 713 2287 6895 Fragments arranged by size: 6895 2287 713 BstYI r'GATC_y Cuts at: 1972 3290 3701 4427 6403 6958 7117 7225 7592 Size: 1318 411 726 1976 555 159 108 367 Cuts at: 7592 7977 7994 9446 9532 9544 9890 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1976 1452 1318 726 555 411 385 367 346 159 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2811 3948 4623 2811 Size: 1137 675 8083 Fragments arranged by size: 8083 1137 675 BtgI C'CryG_G Cuts at: 513 1065 3186 6143 6963 513 Size: 552 2121 2957 820 3445 Fragments arranged by size: 3445 2957 2121 820 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2187 5180 487 Size: 683 56 961 2993 5202 Fragments arranged by size: 5202 2993 961 683 56 BtsI GCAGTG_nn' Cuts at: 2250 4954 5648 6707 6741 6794 7016 7411 8205 Size: 2704 694 1059 34 53 222 395 794 Cuts at: 8205 8225 2250 Size: 20 3920 Fragments arranged by size: 3920 2704 1059 794 694 395 222 53 34 20 ClaI AT'CG_AT Cuts at: 1554 5206 1554 Size: 3652 6243 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9860 DraI TTT'AAA Cuts at: 5878 7199 5878 Size: 1321 8574 DraIII CAC_nnn'GTG Cuts at: 2329 3921 6026 6311 2329 Size: 1592 2105 285 5913 Fragments arranged by size: 5913 2105 1592 285 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4871 6185 8902 809 Size: 507 3555 1314 2717 1802 Fragments arranged by size: 3555 2717 1802 1314 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2954 3787 Size: 54 1425 50 275 18 345 779 833 Cuts at: 3787 6028 6777 6943 7342 8231 9860 8 Size: 2241 749 166 399 889 1629 43 Fragments arranged by size: 2241 1629 1425 889 833 779 749 399 345 275 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2175 7342 1537 Size: 275 363 5167 4090 Fragments arranged by size: 5167 4090 363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2610 2699 3927 4144 4644 4896 7825 1353 Size: 1257 89 1228 217 500 252 2929 3423 Fragments arranged by size: 3423 2929 1257 1228 500 252 217 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8482 8856 9002 387 Size: 1064 138 6893 374 146 1280 Fragments arranged by size: 6893 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4069 4883 4069 Size: 814 9081 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2433 2902 3217 3285 3648 4548 4615 Size: 393 801 469 315 68 363 900 67 Cuts at: 4615 4686 5109 5797 6105 6348 6448 6550 6622 Size: 71 423 688 308 243 100 102 72 Cuts at: 6622 6956 7958 8555 9342 9610 1239 Size: 334 1002 597 787 268 1524 Fragments arranged by size: 1524 1002 900 801 787 688 597 469 423 393 363 334 315 308 268 243 102 100 72 71 68 67 EcoO109I rG'GnC_Cy Cuts at: 2390 3311 3468 4771 5465 5466 6038 6968 7070 Size: 921 157 1303 694 1 572 930 102 Cuts at: 7070 7071 2390 Size: 1 5214 Fragments arranged by size: 5214 1303 930 921 694 572 157 102 1 1 EcoRI G'AATT_C Cuts at: 4759 6125 7204 7582 4759 Size: 1366 1079 378 7072 Fragments arranged by size: 7072 1366 1079 378 EcoRV GAT'ATC Cuts at: 5191 6004 5191 Size: 813 9082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4413 4413 Size: 9895 FspI TGC'GCA Cuts at: 8401 8401 Size: 9895 HaeII r_GCGC'y Cuts at: 1678 2271 2450 3990 5952 7026 7059 9042 1678 Size: 593 179 1540 1962 1074 33 1983 2531 Fragments arranged by size: 2531 1983 1962 1540 1074 593 179 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2633 2665 3317 3349 3941 Size: 32 135 32 1061 32 652 32 592 Cuts at: 3941 3973 4820 4852 5029 5061 5188 5220 5434 Size: 32 847 32 177 32 127 32 214 Cuts at: 5434 5466 6158 6190 6371 6403 6599 6631 6706 Size: 32 692 32 181 32 196 32 75 Cuts at: 6706 6738 8536 8568 8610 8642 1373 Size: 32 1798 32 42 32 2626 Fragments arranged by size: 2626 1798 1061 847 692 652 592 214 196 181 177 135 127 75 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6675 7239 7485 669 Size: 988 281 4737 564 246 3079 Fragments arranged by size: 4737 3079 988 564 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9895 HpaI GTT'AAC Cuts at: 7485 7485 Size: 9895 KpnI G_GTAC'C Cuts at: 5744 7220 5744 Size: 1476 8419 MfeI C'AATT_G Cuts at: 2187 4300 7494 2187 Size: 2113 3194 4588 Fragments arranged by size: 4588 3194 2113 MluI A'CGCG_T Cuts at: 9771 9771 Size: 9895 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2571 2859 3268 3709 4167 4655 6154 6459 Size: 1266 288 409 441 458 488 1499 305 Cuts at: 6459 7158 9009 9193 1305 Size: 699 1851 184 2007 Fragments arranged by size: 2007 1851 1499 1266 699 488 458 441 409 305 288 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2956 6030 10 Size: 54 1425 343 1124 3074 3875 Fragments arranged by size: 3875 3074 1425 1124 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4805 5333 5943 6804 6806 7853 8212 Size: 648 3639 528 610 861 2 1047 359 Cuts at: 8212 8371 518 Size: 159 2042 Fragments arranged by size: 3639 2042 1047 861 648 610 528 359 159 2 MspA1I CmG'CkG Cuts at: 1606 4593 4602 4896 5629 6481 6917 7038 7311 Size: 2987 9 294 733 852 436 121 273 Cuts at: 7311 7988 9136 9381 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2987 2120 1148 852 733 677 436 294 273 245 121 9 NcoI C'CATG_G Cuts at: 513 1065 3186 6143 513 Size: 552 2121 2957 4265 Fragments arranged by size: 4265 2957 2121 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9895 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9895 NotI GC'GGCC_GC Cuts at: 7342 7342 Size: 9895 NspI r_CATG'y Cuts at: 1132 1229 2284 2371 5538 6697 6805 7253 8798 Size: 97 1055 87 3167 1159 108 448 1545 Cuts at: 8798 1132 Size: 2229 Fragments arranged by size: 3167 2229 1545 1159 1055 448 108 97 87 PasI CC'CwG_GG Cuts at: 1348 3867 6736 1348 Size: 2519 2869 4507 Fragments arranged by size: 4507 2869 2519 PciI A'CATG_T Cuts at: 6801 8794 6801 Size: 1993 7902 PflMI CCAn_nnn'nTGG Cuts at: 1108 2216 3146 4467 4644 5795 6497 7129 1108 Size: 1108 930 1321 177 1151 702 632 3874 Fragments arranged by size: 3874 1321 1151 1108 930 702 632 177 PfoI T'CCnGG_A Cuts at: 3233 3389 3470 4466 6464 3233 Size: 156 81 996 1998 6664 Fragments arranged by size: 6664 1998 996 156 81 PmeI GTTT'AAAC Cuts at: 7199 7199 Size: 9895 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2681 2713 3390 3422 4121 4153 5854 5886 7964 Size: 32 677 32 699 32 1701 32 2078 Cuts at: 7964 7996 9477 9509 2681 Size: 32 1481 32 3067 Fragments arranged by size: 3067 2078 1701 1481 699 677 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3468 6038 6968 3468 Size: 2570 930 6395 Fragments arranged by size: 6395 2570 930 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9895 PsiI TTA'TAA Cuts at: 4728 7465 4728 Size: 2737 7158 PspOMI G'GGCC_C Cuts at: 4771 5465 5714 7070 4771 Size: 694 249 1356 7596 Fragments arranged by size: 7596 1356 694 249 PstI C_TGCA'G Cuts at: 830 2457 5655 6898 7247 830 Size: 1627 3198 1243 349 3478 Fragments arranged by size: 3478 3198 1627 1243 349 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9895 PvuII CAG'CTG Cuts at: 4593 4896 5629 6917 4593 Size: 303 733 1288 7571 Fragments arranged by size: 7571 1288 733 303 SacI G_AGCT'C Cuts at: 4071 4885 4071 Size: 814 9081 SalI G'TCGA_C Cuts at: 1936 7237 1936 Size: 5301 4594 SapI GCTCTTCn'nnn_ Cuts at: 2699 4644 2699 Size: 1945 7950 SbfI CC_TGCA'GG Cuts at: 7247 7247 Size: 9895 ScaI AGT'ACT Cuts at: 8143 9855 8143 Size: 1712 8183 SexAI A'CCwGG_T Cuts at: 6307 7153 6307 Size: 846 9049 SfcI C'TryA_G Cuts at: 826 1045 2453 2844 4080 5121 5651 5913 5952 Size: 219 1408 391 1236 1041 530 262 39 Cuts at: 5952 6322 6894 7243 8378 9059 9250 826 Size: 370 572 349 1135 681 191 1471 Fragments arranged by size: 1471 1408 1236 1135 1041 681 572 530 391 370 349 262 219 191 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9895 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9895 SmaI CCC'GGG Cuts at: 1872 5975 7222 1872 Size: 4103 1247 4545 Fragments arranged by size: 4545 4103 1247 SmlI C'TyrA_G Cuts at: 820 1017 1943 3169 4782 5417 5598 6679 7999 Size: 197 926 1226 1613 635 181 1081 1320 Cuts at: 7999 8900 9162 820 Size: 901 262 1553 Fragments arranged by size: 1613 1553 1320 1226 1081 926 901 635 262 197 181 SnaBI TAC'GTA Cuts at: 493 7192 7686 493 Size: 6699 494 2702 Fragments arranged by size: 6699 2702 494 SpeI A'CTAG_T Cuts at: 152 2729 152 Size: 2577 7318 SphI G_CATG'C Cuts at: 1132 2371 6697 7253 1132 Size: 1239 4326 556 3774 Fragments arranged by size: 4326 3774 1239 556 SspI AAT'ATT Cuts at: 5 52 7819 5 Size: 47 7767 2081 Fragments arranged by size: 7767 2081 47 StuI AGG'CCT Cuts at: 1517 3946 1517 Size: 2429 7466 StyI C'CwwG_G Cuts at: 513 1065 2374 2488 3186 3670 3941 4765 6143 Size: 552 1309 114 698 484 271 824 1378 Cuts at: 6143 6780 7334 513 Size: 637 554 3074 Fragments arranged by size: 3074 1378 1309 824 698 637 554 552 484 271 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3783 4045 4323 4398 6014 7922 7939 Size: 1255 2203 262 278 75 1616 1908 17 Cuts at: 7939 8092 8277 8722 325 Size: 153 185 445 1498 Fragments arranged by size: 2203 1908 1616 1498 1255 445 278 262 185 153 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 8141 9853 96 Size: 274 80 33 51 1443 6164 1712 138 Fragments arranged by size: 6164 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2869 2901 3905 3937 4199 4231 6595 6627 9855 Size: 32 1004 32 262 32 2364 32 3228 Cuts at: 9855 9887 2869 Size: 32 2877 Fragments arranged by size: 3228 2877 2364 1004 262 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3329 3568 1316 Size: 2013 239 7643 Fragments arranged by size: 7643 2013 239 XbaI T'CTAG_A Cuts at: 7231 7231 Size: 9895 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2577 5725 6141 6525 6921 2577 Size: 3148 416 384 396 5551 Fragments arranged by size: 5551 3148 416 396 384 XhoI C'TCGA_G Cuts at: 1943 4782 1943 Size: 2839 7056 XmaI C'CCGG_G Cuts at: 1870 5973 7220 1870 Size: 4103 1247 4545 Fragments arranged by size: 4545 4103 1247 XmnI GAAnn'nnTTC Cuts at: 3703 5131 5593 8024 3703 Size: 1428 462 2431 5574 Fragments arranged by size: 5574 2431 1428 462 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7703 276 Size: 53 83 186 936 6169 2468 Fragments arranged by size: 6169 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI