(Circular) (Six-base) MAPSORT of: pFN21ASDA1088.seq Check: 4592 from: 1 to: 8929 pFN21ASDA1088 8929 bp With 182 enzymes: SgfI * February 1, 2012 16:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2856 6739 278 Size: 53 83 186 936 1320 3883 2468 Fragments arranged by size: 3883 2468 1320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2055 6272 1937 Size: 118 4217 4594 Fragments arranged by size: 4594 4217 118 Acc65I G'GTAC_C Cuts at: 4068 6250 4068 Size: 2182 6747 AclI AA'CG_TT Cuts at: 1563 7056 7429 1563 Size: 5493 373 3063 Fragments arranged by size: 5493 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2687 2768 2826 2996 3102 3379 3797 4439 Size: 1055 81 58 170 106 277 418 642 Cuts at: 4439 5825 6992 8376 8644 1632 Size: 1386 1167 1384 268 1917 Fragments arranged by size: 1917 1386 1384 1167 1055 642 418 277 268 170 106 81 58 AfeI AGC'GCT Cuts at: 5268 5402 5813 6189 5268 Size: 134 411 376 8008 Fragments arranged by size: 8008 411 376 134 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8732 AflIII A'CryG_T Cuts at: 1203 6135 7828 8805 1203 Size: 4932 1693 977 1327 Fragments arranged by size: 4932 1693 1327 977 AgeI A'CCGG_T Cuts at: 7760 7760 Size: 8929 AhdI GACnn_n'nnGTC Cuts at: 2852 7658 2852 Size: 4806 4123 AleI CACnn'nnGTG Cuts at: 1166 5413 6099 1166 Size: 4247 686 3996 Fragments arranged by size: 4247 3996 686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5396 6117 1184 Size: 4212 721 3996 Fragments arranged by size: 4212 3996 721 AlwNI CAG_nnn'CTG Cuts at: 2581 2869 3148 3196 4009 4499 4777 5068 5597 Size: 288 279 48 813 490 278 291 529 Cuts at: 5597 5666 6672 8244 2581 Size: 69 1006 1572 3266 Fragments arranged by size: 3266 1572 1006 813 529 490 291 288 279 278 69 48 ApaI G_GGCC'C Cuts at: 3748 5233 5733 3748 Size: 1485 500 6944 Fragments arranged by size: 6944 1485 500 ApaLI G'TGCA_C Cuts at: 2182 3290 4692 5416 6986 8142 2182 Size: 1108 1402 724 1570 1156 2969 Fragments arranged by size: 2969 1570 1402 1156 1108 724 ApoI r'AATT_y Cuts at: 1493 6238 6464 6616 8725 1493 Size: 4745 226 152 2109 1697 Fragments arranged by size: 4745 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7483 160 Size: 872 6451 1606 Fragments arranged by size: 6451 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8929 AvaI C'yCGr_G Cuts at: 1870 1943 2442 3984 4305 4457 5443 6254 1870 Size: 73 499 1542 321 152 986 811 4545 Fragments arranged by size: 4545 1542 986 811 499 321 152 73 BamHI G'GATC_C Cuts at: 5029 6259 6626 5029 Size: 1230 367 7332 Fragments arranged by size: 7332 1230 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3676 3869 4068 4122 5023 Size: 325 217 627 1889 193 199 54 901 Cuts at: 5023 5779 5835 5999 6093 6250 7705 618 Size: 756 56 164 94 157 1455 1842 Fragments arranged by size: 1889 1842 1455 901 756 627 325 217 199 193 164 157 94 56 54 BanII G_rGCy'C Cuts at: 1391 2150 2441 2907 3592 3602 3690 3748 3798 Size: 759 291 466 685 10 88 58 50 Cuts at: 3798 4189 4565 4683 5233 5512 5722 5733 5919 Size: 391 376 118 550 279 210 11 186 Cuts at: 5919 6248 1391 Size: 329 4072 Fragments arranged by size: 4072 759 685 550 466 391 376 329 291 279 210 186 118 88 58 50 11 10 BbeI G_GCGC'C Cuts at: 3873 5027 3873 Size: 1154 7775 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 2967 4138 5546 5555 5896 928 Size: 971 272 796 1171 1408 9 341 3961 Fragments arranged by size: 3961 1408 1171 971 796 341 272 9 BbvCI CC'TCA_GC Cuts at: 4842 4842 Size: 8929 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7120 7154 1903 Size: 34 5183 34 3678 Fragments arranged by size: 5183 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4937 6821 8031 4937 Size: 1884 1210 5835 Fragments arranged by size: 5835 1884 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8929 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2246 3685 4307 Size: 107 122 71 631 605 574 1439 622 Cuts at: 4307 4519 4814 5213 5228 6091 7540 136 Size: 212 295 399 15 863 1449 1525 Fragments arranged by size: 1525 1449 1439 863 631 622 605 574 399 295 212 122 107 71 15 BglII A'GATC_T Cuts at: 3552 5562 8924 3552 Size: 2010 3362 3557 Fragments arranged by size: 3557 3362 2010 BlpI GC'TnA_GC Cuts at: 3327 3380 6346 3327 Size: 53 2966 5910 Fragments arranged by size: 5910 2966 53 Bme1580I G_kGCm'C Cuts at: 1790 2186 2645 3294 3681 3748 4696 4993 5172 Size: 396 459 649 387 67 948 297 179 Cuts at: 5172 5233 5420 5733 5784 5795 5861 6087 6990 Size: 61 187 313 51 11 66 226 903 Cuts at: 6990 8146 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 948 903 649 459 396 387 313 297 226 187 179 67 66 61 51 11 BmgBI CAC'GTC Cuts at: 1318 3158 4216 4255 5415 5776 6136 1318 Size: 1840 1058 39 1160 361 360 4111 Fragments arranged by size: 4111 1840 1160 1058 361 360 39 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3506 3586 4255 Size: 416 53 83 399 24 2089 80 669 Cuts at: 4255 4314 6074 6660 7618 442 Size: 59 1760 586 958 1753 Fragments arranged by size: 2089 1760 1753 958 669 586 416 399 83 80 59 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8929 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2225 3342 2225 Size: 1117 7812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 2331 2344 2591 2891 3017 3048 3183 Size: 923 169 13 247 300 126 31 135 Cuts at: 3183 3364 3512 3542 3752 4556 5184 5863 5961 Size: 181 148 30 210 804 628 679 98 Cuts at: 5961 6204 7589 1239 Size: 243 1385 2579 Fragments arranged by size: 2579 1385 923 804 679 628 300 247 243 210 181 169 148 135 126 98 31 30 13 Bpu10I CC'TnA_GC Cuts at: 4842 4962 4842 Size: 120 8809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3980 7054 7919 8217 3980 Size: 3074 865 298 4692 Fragments arranged by size: 4692 3074 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2307 3815 5127 7592 882 Size: 617 808 1508 1312 2465 2219 Fragments arranged by size: 2465 2219 1508 1312 808 617 BsaAI yAC'GTr Cuts at: 493 1204 6226 6720 493 Size: 711 5022 494 2702 Fragments arranged by size: 5022 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3500 8923 3500 Size: 5423 3506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2853 3435 3870 4082 Size: 53 83 186 936 1320 582 435 212 Cuts at: 4082 5024 6736 7118 7753 275 Size: 942 1712 382 635 1451 Fragments arranged by size: 1712 1451 1320 942 936 635 582 435 382 212 186 83 53 BsaWI w'CCGG_w Cuts at: 3315 7362 7760 8034 8181 8592 3315 Size: 4047 398 274 147 411 3652 Fragments arranged by size: 4047 3652 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 2780 2810 4574 Size: 30 165 30 1035 30 312 30 1764 Cuts at: 4574 4604 5704 5734 5861 5891 6210 6240 1178 Size: 30 1100 30 127 30 319 30 3867 Fragments arranged by size: 3867 1764 1100 1035 319 312 165 127 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2888 3641 4586 4653 4691 4931 5486 1186 Size: 1702 753 945 67 38 240 555 4629 Fragments arranged by size: 4629 1702 945 753 555 240 67 38 BseYI C'CCAG_C Cuts at: 2434 2933 3256 3265 4037 4412 5061 5627 5699 Size: 499 323 9 772 375 649 566 72 Cuts at: 5699 5860 8132 8628 2434 Size: 161 2272 496 2735 Fragments arranged by size: 2735 2272 772 649 566 499 496 375 323 161 72 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5345 6123 1163 Size: 573 3609 778 3969 Fragments arranged by size: 3969 3609 778 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3183 5434 6111 6379 7140 8168 Size: 275 186 1182 2251 677 268 761 1028 Cuts at: 8168 1540 Size: 2301 Fragments arranged by size: 2301 2251 1182 1028 761 677 275 268 186 BsiHKAI G_wGCw'C Cuts at: 2186 2734 3294 3350 3602 4542 4696 4968 5085 Size: 548 560 56 252 940 154 272 117 Cuts at: 5085 5420 5722 6990 7075 8146 2186 Size: 335 302 1268 85 1071 2969 Fragments arranged by size: 2969 1268 1071 940 560 548 335 302 272 252 154 117 85 56 BsiWI C'GTAC_G Cuts at: 6222 6222 Size: 8929 BsmI GAATG_Cn' Cuts at: 4971 6440 6533 4971 Size: 1469 93 7367 Fragments arranged by size: 7367 1469 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2208 3024 4245 2208 Size: 816 1221 6892 Fragments arranged by size: 6892 1221 816 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2186 2441 2645 2734 2907 3294 Size: 399 360 36 255 204 89 173 387 Cuts at: 3294 3350 3592 3602 3681 3690 3748 3798 4189 Size: 56 242 10 79 9 58 50 391 Cuts at: 4189 4542 4565 4683 4696 4968 4993 5085 5172 Size: 353 23 118 13 272 25 92 87 Cuts at: 5172 5233 5420 5512 5722 5733 5784 5795 5861 Size: 61 187 92 210 11 51 11 66 Cuts at: 5861 5919 6087 6248 6990 7075 8146 1391 Size: 58 168 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 387 360 353 272 255 242 210 204 187 173 168 161 118 92 92 89 87 85 79 66 61 58 58 56 51 50 36 25 23 13 11 11 10 9 BspEI T'CCGG_A Cuts at: 8592 8592 Size: 8929 BspHI T'CATG_A Cuts at: 6818 8548 6818 Size: 1730 7199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4410 4872 5612 5890 6284 844 Size: 3566 462 740 278 394 3489 Fragments arranged by size: 3566 3489 740 462 394 278 BsrBI CCG'CTC Cuts at: 1394 1823 2622 3441 6065 6816 1394 Size: 429 799 819 2624 751 3507 Fragments arranged by size: 3507 2624 819 799 751 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2964 7424 7598 65 Size: 1183 1716 4460 174 1396 Fragments arranged by size: 4460 1716 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2415 3866 4071 4297 4621 4996 5429 Size: 110 605 1451 205 226 324 375 433 Cuts at: 5429 5433 5996 6002 7573 7760 1700 Size: 4 563 6 1571 187 2869 Fragments arranged by size: 2869 1571 1451 605 563 433 375 324 226 205 187 110 6 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8929 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8929 BssSI C'ACGA_G Cuts at: 3416 6989 8001 3416 Size: 3573 1012 4344 Fragments arranged by size: 4344 3573 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3158 3848 4864 794 Size: 2364 690 1016 4859 Fragments arranged by size: 4859 2364 1016 690 BstBI TT'CG_AA Cuts at: 2007 7733 2007 Size: 5726 3203 BstEII G'GTnAC_C Cuts at: 1184 3311 4437 1184 Size: 2127 1126 5676 Fragments arranged by size: 5676 2127 1126 BstXI CCAn_nnnn'nTGG Cuts at: 2107 3766 3880 5762 2107 Size: 1659 114 1882 5274 Fragments arranged by size: 5274 1882 1659 114 BstYI r'GATC_y Cuts at: 1972 2499 3501 3552 4653 5029 5562 6259 6626 Size: 527 1002 51 1101 376 533 697 367 Cuts at: 6626 7011 7028 8480 8566 8578 8924 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1101 1002 697 533 527 385 376 367 346 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2421 2466 4432 5638 2421 Size: 45 1966 1206 5712 Fragments arranged by size: 5712 1966 1206 45 BtgI C'CryG_G Cuts at: 513 1065 3937 4683 5049 5241 5650 5712 513 Size: 552 2872 746 366 192 409 62 3730 Fragments arranged by size: 3730 2872 746 552 409 366 192 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3649 5680 487 Size: 683 56 2423 2031 3736 Fragments arranged by size: 3736 2423 2031 683 56 BtsI GCAGTG_nn' Cuts at: 3351 5331 6445 7239 7259 3351 Size: 1980 1114 794 20 5021 Fragments arranged by size: 5021 1980 1114 794 20 ClaI AT'CG_AT Cuts at: 1554 3009 1554 Size: 1455 7474 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8894 DraI TTT'AAA Cuts at: 6233 6233 Size: 8929 DraIII CAC_nnn'GTG Cuts at: 2638 2785 4322 5200 6136 2638 Size: 147 1537 878 936 5431 Fragments arranged by size: 5431 1537 936 878 147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3041 4406 7936 809 Size: 507 1725 1365 3530 1802 Fragments arranged by size: 3530 1802 1725 1365 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2583 3180 3403 Size: 54 1425 50 275 18 753 597 223 Cuts at: 3403 3756 3934 4237 4527 4725 4815 5319 5431 Size: 353 178 303 290 198 90 504 112 Cuts at: 5431 5909 6108 6376 7265 8894 8 Size: 478 199 268 889 1629 43 Fragments arranged by size: 1629 1425 889 753 597 504 478 353 303 290 275 268 223 199 198 178 112 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3180 5431 6108 6376 1537 Size: 275 1368 2251 677 268 4090 Fragments arranged by size: 4090 2251 1368 677 275 268 EarI CTCTTCn'nnn_ Cuts at: 1353 3719 4610 4721 6859 1353 Size: 2366 891 111 2138 3423 Fragments arranged by size: 3423 2366 2138 891 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3215 3416 7516 7890 8036 387 Size: 1064 138 1626 201 4100 374 146 1280 Fragments arranged by size: 4100 1626 1280 1064 374 201 146 138 EcoICRI GAG'CTC Cuts at: 3600 5720 3600 Size: 2120 6809 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2331 2344 2591 2687 2768 2826 Size: 393 530 169 13 247 96 81 58 Cuts at: 2826 2891 2996 3017 3048 3102 3183 3364 3379 Size: 65 105 21 31 54 81 181 15 Cuts at: 3379 3512 3542 3752 3797 4439 4556 5184 5825 Size: 133 30 210 45 642 117 628 641 Cuts at: 5825 5863 5961 6204 6992 7589 8376 8644 1239 Size: 38 98 243 788 597 787 268 1524 Fragments arranged by size: 1524 788 787 642 641 628 597 530 393 268 247 243 210 181 169 133 117 105 98 96 81 81 65 58 54 45 38 31 30 21 15 13 EcoNI CCTnn'n_nnAGG Cuts at: 2573 3647 5090 5300 2573 Size: 1074 1443 210 6202 Fragments arranged by size: 6202 1443 1074 210 EcoO109I rG'GnC_Cy Cuts at: 2104 2364 3270 3578 4428 4453 4774 4846 5100 Size: 260 906 308 850 25 321 72 254 Cuts at: 5100 5229 5230 5462 5522 5624 5642 5730 2104 Size: 129 1 232 60 102 18 88 5303 Fragments arranged by size: 5303 906 850 321 308 260 254 232 129 102 88 72 60 25 18 1 EcoRI G'AATT_C Cuts at: 6238 6616 6238 Size: 378 8551 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2276 5419 2276 Size: 3143 5786 FseI GG_CCGG'CC Cuts at: 5437 5437 Size: 8929 FspI TGC'GCA Cuts at: 7435 7435 Size: 8929 HaeII r_GCGC'y Cuts at: 1678 3776 3873 5027 5208 5270 5404 5815 5940 Size: 2098 97 1154 181 62 134 411 125 Cuts at: 5940 5982 6191 8076 1678 Size: 42 209 1885 2531 Fragments arranged by size: 2531 2098 1885 1154 411 209 181 134 125 97 62 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2205 2207 2237 2239 2476 Size: 32 135 32 633 2 30 2 237 Cuts at: 2476 2508 2838 2870 3620 3652 4340 4372 7570 Size: 32 330 32 750 32 688 32 3198 Cuts at: 7570 7602 7644 7676 1373 Size: 32 42 32 2626 Fragments arranged by size: 3198 2626 750 688 633 330 237 135 42 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 6273 6519 669 Size: 988 281 4335 246 3079 Fragments arranged by size: 4335 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8929 HpaI GTT'AAC Cuts at: 6519 6519 Size: 8929 KasI G'GCGC_C Cuts at: 3869 5023 3869 Size: 1154 7775 KpnI G_GTAC'C Cuts at: 4072 6254 4072 Size: 2182 6747 MfeI C'AATT_G Cuts at: 6528 6528 Size: 8929 MluI A'CGCG_T Cuts at: 8805 8805 Size: 8929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2691 8043 8227 1305 Size: 1386 5352 184 2007 Fragments arranged by size: 5352 2007 1386 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2585 3758 4239 5911 10 Size: 54 1425 343 753 1173 481 1672 3028 Fragments arranged by size: 3028 1672 1425 1173 753 481 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 3303 5378 5413 5668 6099 6887 Size: 648 1524 613 2075 35 255 431 788 Cuts at: 6887 7246 7405 518 Size: 359 159 2042 Fragments arranged by size: 2075 2042 1524 788 648 613 431 359 255 159 35 MspA1I CmG'CkG Cuts at: 1606 2452 3039 3331 3459 3784 4445 4552 4587 Size: 846 587 292 128 325 661 107 35 Cuts at: 4587 4933 4987 5068 5243 6107 6345 7022 8170 Size: 346 54 81 175 864 238 677 1148 Cuts at: 8170 8415 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 864 846 677 661 587 346 325 292 245 238 175 128 107 81 54 35 NaeI GCC'GGC Cuts at: 4623 5435 6004 4623 Size: 812 569 7548 Fragments arranged by size: 7548 812 569 NarI GG'CG_CC Cuts at: 3870 5024 3870 Size: 1154 7775 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8377 NdeI CA'TA_TG Cuts at: 387 387 Size: 8929 NgoMIV G'CCGG_C Cuts at: 4621 5433 6002 4621 Size: 812 569 7548 Fragments arranged by size: 7548 812 569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8929 NotI GC'GGCC_GC Cuts at: 6376 6376 Size: 8929 NspI r_CATG'y Cuts at: 1132 1229 3415 4409 5377 6287 7832 1132 Size: 97 2186 994 968 910 1545 2229 Fragments arranged by size: 2229 2186 1545 994 968 910 97 PasI CC'CwG_GG Cuts at: 1348 2939 3643 3857 4515 5233 1348 Size: 1591 704 214 658 718 5044 Fragments arranged by size: 5044 1591 718 704 658 214 PciI A'CATG_T Cuts at: 7828 7828 Size: 8929 PflMI CCAn_nnn'nTGG Cuts at: 1108 2836 2869 3148 5068 5260 1108 Size: 1728 33 279 1920 192 4777 Fragments arranged by size: 4777 1920 1728 279 192 33 PfoI T'CCnGG_A Cuts at: 2106 2139 2265 2360 2595 2657 3117 4008 5001 Size: 33 126 95 235 62 460 891 993 Cuts at: 5001 5879 2106 Size: 878 5156 Fragments arranged by size: 5156 993 891 878 460 235 126 95 62 33 PmeI GTTT'AAAC Cuts at: 6233 6233 Size: 8929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6998 7030 8511 8543 6998 Size: 32 1481 32 7384 Fragments arranged by size: 7384 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2364 4428 4453 4846 5462 5624 5642 2104 Size: 260 2064 25 393 616 162 18 5391 Fragments arranged by size: 5391 2064 616 393 260 162 25 18 PshAI GACnn'nnGTC Cuts at: 1613 2102 1613 Size: 489 8440 PsiI TTA'TAA Cuts at: 6499 6499 Size: 8929 PspOMI G'GGCC_C Cuts at: 3744 5229 5729 3744 Size: 1485 500 6944 Fragments arranged by size: 6944 1485 500 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2396 2428 2396 Size: 32 8897 PstI C_TGCA'G Cuts at: 830 2121 3192 3358 4782 5059 5662 6281 830 Size: 1291 1071 166 1424 277 603 619 3478 Fragments arranged by size: 3478 1424 1291 1071 619 603 277 166 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8929 PvuII CAG'CTG Cuts at: 2452 4445 4587 4933 5068 2452 Size: 1993 142 346 135 6313 Fragments arranged by size: 6313 1993 346 142 135 RsrII CG'GwC_CG Cuts at: 5532 5532 Size: 8929 SacI G_AGCT'C Cuts at: 3602 5722 3602 Size: 2120 6809 SacII CC_GC'GG Cuts at: 5244 5244 Size: 8929 SalI G'TCGA_C Cuts at: 1936 6271 1936 Size: 4335 4594 SanDI GG'GwC_CC Cuts at: 4453 5462 4453 Size: 1009 7920 SapI GCTCTTCn'nnn_ Cuts at: 3719 4721 3719 Size: 1002 7927 SbfI CC_TGCA'GG Cuts at: 4782 6281 4782 Size: 1499 7430 ScaI AGT'ACT Cuts at: 7177 8889 7177 Size: 1712 7217 SexAI A'CCwGG_T Cuts at: 2862 2862 Size: 8929 SfcI C'TryA_G Cuts at: 826 1045 2117 2495 3188 3354 4311 4778 5055 Size: 219 1072 378 693 166 957 467 277 Cuts at: 5055 5248 5658 6277 7412 8093 8284 826 Size: 193 410 619 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 957 693 681 619 467 410 378 277 219 193 191 166 SfoI GGC'GCC Cuts at: 3871 5025 3871 Size: 1154 7775 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8929 SgrAI Cr'CCGG_yG Cuts at: 1700 3866 1700 Size: 2166 6763 SmaI CCC'GGG Cuts at: 1872 2444 3986 4307 5445 6256 1872 Size: 572 1542 321 1138 811 4545 Fragments arranged by size: 4545 1542 1138 811 572 321 SmlI C'TyrA_G Cuts at: 820 1017 1943 3995 7033 7934 8196 820 Size: 197 926 2052 3038 901 262 1553 Fragments arranged by size: 3038 2052 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6226 6720 493 Size: 5733 494 2702 Fragments arranged by size: 5733 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8929 SphI G_CATG'C Cuts at: 1132 3415 6287 1132 Size: 2283 2872 3774 Fragments arranged by size: 3774 2872 2283 SspI AAT'ATT Cuts at: 5 52 6853 5 Size: 47 6801 2081 Fragments arranged by size: 6801 2081 47 StuI AGG'CCT Cuts at: 1517 5846 5953 1517 Size: 4329 107 4493 Fragments arranged by size: 4493 4329 107 StyI C'CwwG_G Cuts at: 513 1065 2100 2883 3105 4870 6368 513 Size: 552 1035 783 222 1765 1498 3074 Fragments arranged by size: 3074 1765 1498 1035 783 552 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3547 4192 4333 4784 5163 6044 6956 Size: 1255 1967 645 141 451 379 881 912 Cuts at: 6956 6973 7126 7311 7756 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1967 1498 1255 912 881 645 451 445 379 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 7175 8887 Size: 274 80 33 51 1443 114 5084 1712 Cuts at: 8887 96 Size: 138 Fragments arranged by size: 5084 1712 1443 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 2780 2812 4574 4606 4983 5015 5704 Size: 32 310 32 1762 32 377 32 689 Cuts at: 5704 5736 5861 5893 8889 8921 2438 Size: 32 125 32 2996 32 2446 Fragments arranged by size: 2996 2446 1762 689 377 310 125 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2612 5044 1316 Size: 1296 2432 5201 Fragments arranged by size: 5201 2432 1296 XbaI T'CTAG_A Cuts at: 6265 6265 Size: 8929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2593 3305 3463 3910 4034 4052 4522 4717 5936 Size: 712 158 447 124 18 470 195 1219 Cuts at: 5936 2593 Size: 5586 Fragments arranged by size: 5586 1219 712 470 447 195 158 124 18 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8929 XmaI C'CCGG_G Cuts at: 1870 2442 3984 4305 5443 6254 1870 Size: 572 1542 321 1138 811 4545 Fragments arranged by size: 4545 1542 1138 811 572 321 XmnI GAAnn'nnTTC Cuts at: 7058 7058 Size: 8929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2854 6737 276 Size: 53 83 186 936 1320 3883 2468 Fragments arranged by size: 3883 2468 1320 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV FspAI NruI NsiI PacI PmlI PspXI SfiI SrfI SwaI