Restriction map for
pFN21ASDA1087
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI AlwNI AocI ApoI AscI
AseI AsnI Asp718I AvrII BamHI
BbvCI BciVI BclI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MfeI MroI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 238 GTCTAC GT^[AC][GT]AC
AflII 1849 CTTAAG C^TTAAG
AhdI 181 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1711 GTGCAC G^TGCAC
ApaLI 1711 GTGCAC G^TGCAC
Asp700I 2472 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 181 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 1184 GCATCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1199 CTAG C^TAG
BfrI 1849 CTTAAG C^TTAAG
BsaBI 318 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 1451 ACCTGC ACCTGC(4/8)
BsrBI 2146 GAGCGG CCGCTC(-3/-3)
BsrDI 2607 CATTGC GCAATG(2/0)
BstXI 2399 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1624 GACGTGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 181 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1124 CCTCGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI 1199 CTAG C^TAG
MamI 318 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 233 CCATGG C^CATGG
NdeI 1997 CATATG CA^TATG
PshAI 693 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2416 CTGCAG CTGCA^G
PvuII 997 CAGCTG CAG^CTG
ScaI 2231 AGTACT AGT^ACT
SfcI 2416 CTGCAG C^T[GA][CT]AG
XcmI 1505 CCAAGGGGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2472 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Aor51HI 502 AGCGCT AGC^GCT
773 AGCGCT
AspHI 1711 GTGCAC G[AT]GC[AT]^C
2203 GTGCTC
AspI 378 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2341 GACTCTGTC
BalI 230 TGGCCA TGG^CCA
1532 TGGCCA
BbrPI 1248 CACGTG CAC^GTG
1597 CACGTG
BsaI 343 GAGACC GGTCTC(1/5)
1756 GAGACC
BsiHKAI 1711 GTGCAC G[AT]GC[AT]^C
2203 GTGCTC
BsmBI 376 GAGACG CGTCTC(1/5)
1369 GAGACG
Bsp1407I 767 TGTACA T^GTACA
2714 TGTACA
BspLU11I 242 ACATGT A^CATGT
515 ACATGT
BsrGI 767 TGTACA T^GTACA
2714 TGTACA
EagI 1142 CGGCCG C^GGCCG
2632 CGGCCG
EclXI 1142 CGGCCG C^GGCCG
2632 CGGCCG
Eco47III 502 AGCGCT AGC^GCT
773 AGCGCT
Eco52I 1142 CGGCCG C^GGCCG
2632 CGGCCG
MluI 1724 ACGCGT A^CGCGT
2435 ACGCGT
MluNI 230 TGGCCA TGG^CCA
1532 TGGCCA
MscI 230 TGGCCA TGG^CCA
1532 TGGCCA
MseI 1850 TTAA T^TAA
2006 TTAA
PflFI 378 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2341 GACTCTGTC
PflMI 1209 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1951 CCAGTTCTTGG
PmaCI 1248 CACGTG CAC^GTG
1597 CACGTG
PmlI 1248 CACGTG CAC^GTG
1597 CACGTG
SmaI 897 CCCGGG CCC^GGG
2138 CCCGGG
SspBI 767 TGTACA T^GTACA
2714 TGTACA
Tru9I 1850 TTAA T^TAA
2006 TTAA
Tth111I 378 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2341 GACTCTGTC
Van91I 1209 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1951 CCAGTTCTTGG
XmaCI 897 CCCGGG C^CCGGG
2138 CCCGGG
XmaI 897 CCCGGG C^CCGGG
2138 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AvaI AvaII
AviII BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfoI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII