Restriction map for
pFN21ASDA1087
(Circular) (Six-base) MAPSORT of: pFN21ASDA1087.seq Check: 2487 from: 1 to: 7492
pFN21ASDA1087 7492 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5302 278
Size: 53 83 186 936 3766 2468
Fragments arranged by size:
3766 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 4835 1937
Size: 325 2573 4594
Fragments arranged by size:
4594 2573 325
Acc65I G'GTAC_C
Cuts at: 4813 4813
Size: 7492
AclI AA'CG_TT
Cuts at: 1563 5619 5992 1563
Size: 4056 373 3063
Fragments arranged by size:
4056 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3779 5555 6939 7207 1632
Size: 2147 1776 1384 268 1917
Fragments arranged by size:
2147 1917 1776 1384 268
AfeI AGC'GCT
Cuts at: 2527 2798 2527
Size: 271 7221
AflII C'TTAA_G
Cuts at: 820 1017 3872 820
Size: 197 2855 4440
Fragments arranged by size:
4440 2855 197
AflIII A'CryG_T
Cuts at: 1203 2265 2538 2787 3747 4413 4458 4497 6391
Size: 1062 273 249 960 666 45 39 1894
Cuts at: 6391 7368 1203
Size: 977 1327
Fragments arranged by size:
1894 1327 1062 977 960 666 273 249
45 39
AgeI A'CCGG_T
Cuts at: 6323 6323
Size: 7492
AhdI GACnn_n'nnGTC
Cuts at: 2209 6221 2209
Size: 4012 3480
AleI CACnn'nnGTG
Cuts at: 1166 2516 2879 3374 1166
Size: 1350 363 495 5284
Fragments arranged by size:
5284 1350 495 363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4652 1184
Size: 3468 4024
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 2861 2893 3236 3268 2011
Size: 32 818 32 343 32 6235
Fragments arranged by size:
6235 818 343 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5235 6807 5235
Size: 1572 5920
ApaI G_GGCC'C
Cuts at: 2507 2921 2927 2507
Size: 414 6 7072
Fragments arranged by size:
7072 414 6
ApaLI G'TGCA_C
Cuts at: 3734 5549 6705 3734
Size: 1815 1156 4521
Fragments arranged by size:
4521 1815 1156
ApoI r'AATT_y
Cuts at: 1493 4801 5027 5179 7288 1493
Size: 3308 226 152 2109 1697
Fragments arranged by size:
3308 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6046 160
Size: 872 5014 1606
Fragments arranged by size:
5014 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
AvaI C'yCGr_G
Cuts at: 1870 1943 2920 3667 4161 4817 1870
Size: 73 977 747 494 656 4545
Fragments arranged by size:
4545 977 747 656 494 73
BamHI G'GATC_C
Cuts at: 4822 5189 4822
Size: 367 7125
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2378 2510 2834 2885 3110
Size: 325 217 627 591 132 324 51 225
Cuts at: 3110 3160 3197 3544 3638 3680 4397 4650 4813
Size: 50 37 347 94 42 717 253 163
Cuts at: 4813 6268 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 717 627 591 347 325 324
253 225 217 163 132 94 51 50
42 37
BanII G_rGCy'C
Cuts at: 1391 2202 2435 2507 2921 2927 3347 4811 1391
Size: 811 233 72 414 6 420 1464 4072
Fragments arranged by size:
4072 1464 811 420 414 233 72 6
BbeI G_GCGC'C
Cuts at: 2838 2889 3114 3164 3201 3548 4654 2838
Size: 51 225 50 37 347 1106 5676
Fragments arranged by size:
5676 1106 347 225 51 50 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2605 2716 3195 4375 4606 928
Size: 971 706 111 479 1180 231 3814
Fragments arranged by size:
3814 1180 971 706 479 231 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3196 3230 5683 5717 1903
Size: 34 1259 34 2453 34 3678
Fragments arranged by size:
3678 2453 1259 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5384 6594 5384
Size: 1210 6282
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7492
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2756 3282 3532
Size: 107 122 71 631 605 1084 526 250
Cuts at: 3532 3868 6103 136
Size: 336 2235 1525
Fragments arranged by size:
2235 1525 1084 631 605 526 336 250
122 107 71
BglII A'GATC_T
Cuts at: 7487 7487
Size: 7492
BlpI GC'TnA_GC
Cuts at: 4909 4909
Size: 7492
Bme1580I G_kGCm'C
Cuts at: 1790 2381 2507 2921 2927 3371 3641 3738 4245
Size: 591 126 414 6 444 270 97 507
Cuts at: 4245 4400 5553 6709 1790
Size: 155 1153 1156 2573
Fragments arranged by size:
2573 1156 1153 591 507 444 414 270
155 126 97 6
BmgBI CAC'GTC
Cuts at: 1318 2325 3649 1318
Size: 1007 1324 5161
Fragments arranged by size:
5161 1324 1007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3859 4552 5223
Size: 416 53 83 399 24 2442 693 671
Cuts at: 5223 6181 442
Size: 958 1753
Fragments arranged by size:
2442 1753 958 693 671 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3346 3346
Size: 7492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3035 3260 3782 4019 4778 6152 1239
Size: 1796 225 522 237 759 1374 2579
Fragments arranged by size:
2579 1796 1374 759 522 237 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2963 2972 4343 5617 6482 6780 2963
Size: 9 1371 1274 865 298 3675
Fragments arranged by size:
3675 1371 1274 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2360 3773 6155 882
Size: 617 861 1413 2382 2219
Fragments arranged by size:
2382 2219 1413 861 617
BsaAI yAC'GTr
Cuts at: 493 1204 2788 3273 3307 3622 4195 4789 5283
Size: 711 1584 485 34 315 573 594 494
Cuts at: 5283 493
Size: 2702
Fragments arranged by size:
2702 1584 711 594 573 494 485 315
34
BsaBI GATnn'nnATC
Cuts at: 2345 7486 2345
Size: 5141 2351
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2835 2886 2949 3111
Size: 53 83 186 936 1302 51 63 162
Cuts at: 3111 3144 3161 3198 3545 4515 4651 5299 5681
Size: 33 17 37 347 970 136 648 382
Cuts at: 5681 6316 275
Size: 635 1451
Fragments arranged by size:
1451 1302 970 936 648 635 382 347
186 162 136 83 63 53 51 37
33 17
BsaWI w'CCGG_w
Cuts at: 5925 6323 6597 6744 7155 5925
Size: 398 274 147 411 6262
Fragments arranged by size:
6262 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2011 2041 3236 3266 4142
Size: 30 165 30 608 30 1195 30 876
Cuts at: 4142 4172 4316 4346 1178
Size: 30 144 30 4324
Fragments arranged by size:
4324 1195 876 608 165 144 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2425 3455 3950 3953 3956 4028 4154
Size: 859 380 1030 495 3 3 72 126
Cuts at: 4154 4157 4160 4163 4181 1186
Size: 3 3 3 18 4497
Fragments arranged by size:
4497 1030 859 495 380 126 72 18
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2215 2500 2575 2905 3853 4266 4675 6695 7191
Size: 285 75 330 948 413 409 2020 496
Cuts at: 7191 2215
Size: 2516
Fragments arranged by size:
2516 2020 948 496 413 409 330 285
75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3038 3602 4330 4571 1163
Size: 573 1302 564 728 241 4084
Fragments arranged by size:
4084 1302 728 573 564 241
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2296 3168 4658 4942 5703 6731
Size: 275 186 295 872 1490 284 761 1028
Cuts at: 6731 1540
Size: 2301
Fragments arranged by size:
2301 1490 1028 872 761 295 284 275
186
BsiHKAI G_wGCw'C
Cuts at: 3738 4230 5553 5638 6709 3738
Size: 492 1323 85 1071 4521
Fragments arranged by size:
4521 1323 1071 492 85
BsmI GAATG_Cn'
Cuts at: 5003 5096 5003
Size: 93 7399
BsmBI CGTCTCn'nnnn_
Cuts at: 2393 3386 2393
Size: 993 6499
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2381 2435 2507 2921 2927 3347
Size: 399 412 179 54 72 414 6 420
Cuts at: 3347 3371 3641 3738 4230 4245 4400 4811 5553
Size: 24 270 97 492 15 155 411 742
Cuts at: 5553 5638 6709 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 420 414 412 411
399 270 179 155 97 85 72 54
24 15 6
BspEI T'CCGG_A
Cuts at: 7155 7155
Size: 7492
BspHI T'CATG_A
Cuts at: 5381 7111 5381
Size: 1730 5762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3483 4847 844
Size: 2639 1364 3489
Fragments arranged by size:
3489 2639 1364
BsrBI CCG'CTC
Cuts at: 1394 1823 4171 5379 1394
Size: 429 2348 1208 3507
Fragments arranged by size:
3507 2348 1208 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4629 5987 6161 65
Size: 1183 3381 1358 174 1396
Fragments arranged by size:
3381 1396 1358 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2579 3107 3163 3993 4478 4647 4653
Size: 110 769 528 56 830 485 169 6
Cuts at: 4653 6136 6323 1700
Size: 1483 187 2869
Fragments arranged by size:
2869 1483 830 769 528 485 187 169
110 56 6
BsrGI T'GTAC_A
Cuts at: 96 2790 4737 96
Size: 2694 1947 2851
Fragments arranged by size:
2851 2694 1947
BssHII G'CGCG_C
Cuts at: 1925 2936 3672 3674 3719 1925
Size: 1011 736 2 45 5698
Fragments arranged by size:
5698 1011 736 45 2
BssSI C'ACGA_G
Cuts at: 5552 6564 5552
Size: 1012 6480
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7492
BstBI TT'CG_AA
Cuts at: 2007 6296 2007
Size: 4289 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7492
BstXI CCAn_nnnn'nTGG
Cuts at: 4429 4429
Size: 7492
BstYI r'GATC_y
Cuts at: 1972 2448 2973 3010 4822 5189 5574 5591 7043
Size: 476 525 37 1812 367 385 17 1452
Cuts at: 7043 7129 7141 7487 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1812 1452 525 476 385 367 346
86 37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2256 3642 4261 513
Size: 552 957 234 1386 619 3744
Fragments arranged by size:
3744 1386 957 619 552 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2272 2536 3016 487
Size: 683 56 1046 264 480 4963
Fragments arranged by size:
4963 1046 683 480 264 56
BtsI GCAGTG_nn'
Cuts at: 2244 2622 3232 5008 5802 5822 2244
Size: 378 610 1776 794 20 3914
Fragments arranged by size:
3914 1776 794 610 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7492
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7457
DraI TTT'AAA
Cuts at: 4796 4796
Size: 7492
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3653 6499 809
Size: 507 2337 2846 1802
Fragments arranged by size:
2846 2337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2253 2307
Size: 54 1425 50 275 18 288 135 54
Cuts at: 2307 2476 2757 3165 3555 4530 4655 4939 5828
Size: 169 281 408 390 975 125 284 889
Cuts at: 5828 7457 8
Size: 1629 43
Fragments arranged by size:
1629 1425 975 889 408 390 288 284
281 275 169 135 125 54 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3165 4655 4939 1537
Size: 275 1353 1490 284 4090
Fragments arranged by size:
4090 1490 1353 284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2780 2849 3059 3798 4235 4610 4721 4751
Size: 1427 69 210 739 437 375 111 30
Cuts at: 4751 5422 1353
Size: 671 3423
Fragments arranged by size:
3423 1427 739 671 437 375 210 111
69 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3155 6079 6453 6599 387
Size: 1064 138 1566 2924 374 146 1280
Fragments arranged by size:
2924 1566 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3035 3260 3779 3782 4019 4778 5555
Size: 393 1403 225 519 3 237 759 777
Cuts at: 5555 6152 6939 7207 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1403 787 777 759 597 519 393
268 237 225 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3151 3151
Size: 7492
EcoO109I rG'GnC_Cy
Cuts at: 2157 2893 2924 2983 4083 4158 4324 2157
Size: 736 31 59 1100 75 166 5325
Fragments arranged by size:
5325 1100 736 166 75 59 31
EcoRI G'AATT_C
Cuts at: 4801 5179 4801
Size: 378 7114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4246 4246
Size: 7492
FspI TGC'GCA
Cuts at: 3128 3410 3662 4574 5998 3128
Size: 282 252 912 1424 4622
Fragments arranged by size:
4622 1424 912 282 252
FspAI rTGC'GCAy
Cuts at: 4574 4574
Size: 7492
HaeII r_GCGC'y
Cuts at: 1678 2529 2711 2800 2838 2889 3114 3164 3201
Size: 851 182 89 38 51 225 50 37
Cuts at: 3201 3548 4439 4654 6639 1678
Size: 347 891 215 1985 2531
Fragments arranged by size:
2531 1985 891 851 347 225 215 182
89 51 50 38 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2268 2300 2390
Size: 32 135 32 623 32 41 32 90
Cuts at: 2390 2422 3874 3906 4142 4174 4316 4348 4683
Size: 32 1452 32 236 32 142 32 335
Cuts at: 4683 4715 6133 6165 6207 6239 1373
Size: 32 1418 32 42 32 2626
Fragments arranged by size:
2626 1452 1418 623 335 236 142 135
90 42 41 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4836 5082 669
Size: 988 281 2898 246 3079
Fragments arranged by size:
3079 2898 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7492
HpaI GTT'AAC
Cuts at: 5082 5082
Size: 7492
KasI G'GCGC_C
Cuts at: 2834 2885 3110 3160 3197 3544 4650 2834
Size: 51 225 50 37 347 1106 5676
Fragments arranged by size:
5676 1106 347 225 51 50 37
KpnI G_GTAC'C
Cuts at: 4817 4817
Size: 7492
MfeI C'AATT_G
Cuts at: 5091 5091
Size: 7492
MluI A'CGCG_T
Cuts at: 3747 4458 7368 3747
Size: 711 2910 3871
Fragments arranged by size:
3871 2910 711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 3372 4509 6606 6790 1305
Size: 1128 939 1137 2097 184 2007
Fragments arranged by size:
2097 2007 1137 1128 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 3557 10
Size: 54 1425 343 423 1302 3945
Fragments arranged by size:
3945 1425 1302 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 2516 2543 2555 2879 3374 4634
Size: 648 1254 96 27 12 324 495 1260
Cuts at: 4634 5450 5809 5968 518
Size: 816 359 159 2042
Fragments arranged by size:
2042 1260 1254 816 648 495 359 324
159 96 27 12
MspA1I CmG'CkG
Cuts at: 1606 2166 2820 3022 3550 4908 5585 6733 6978
Size: 560 654 202 528 1358 677 1148 245
Cuts at: 6978 1606
Size: 2120
Fragments arranged by size:
2120 1358 1148 677 654 560 528 245
202
NaeI GCC'GGC
Cuts at: 2581 3165 3995 4655 2581
Size: 584 830 660 5418
Fragments arranged by size:
5418 830 660 584
NarI GG'CG_CC
Cuts at: 2835 2886 3111 3161 3198 3545 4651 2835
Size: 51 225 50 37 347 1106 5676
Fragments arranged by size:
5676 1106 347 225 51 50 37
NcoI C'CATG_G
Cuts at: 513 1065 2022 2256 513
Size: 552 957 234 5749
Fragments arranged by size:
5749 957 552 234
NdeI CA'TA_TG
Cuts at: 387 4021 387
Size: 3634 3858
NgoMIV G'CCGG_C
Cuts at: 2579 3163 3993 4653 2579
Size: 584 830 660 5418
Fragments arranged by size:
5418 830 660 584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7492
NotI GC'GGCC_GC
Cuts at: 4939 4939
Size: 7492
NspI r_CATG'y
Cuts at: 1132 1229 2269 2542 3502 4850 6395 1132
Size: 97 1040 273 960 1348 1545 2229
Fragments arranged by size:
2229 1545 1348 1040 960 273 97
PasI CC'CwG_GG
Cuts at: 1348 2428 4393 1348
Size: 1080 1965 4447
Fragments arranged by size:
4447 1965 1080
PciI A'CATG_T
Cuts at: 2265 2538 6391 2265
Size: 273 3853 3366
Fragments arranged by size:
3853 3366 273
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3238 3980 1108
Size: 2130 742 4620
Fragments arranged by size:
4620 2130 742
PfoI T'CCnGG_A
Cuts at: 3570 3603 3570
Size: 33 7459
PmeI GTTT'AAAC
Cuts at: 4796 4796
Size: 7492
PmlI CAC'GTG
Cuts at: 3273 3622 3273
Size: 349 7143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 3236 3268 5561 5593 7074 7106 2011
Size: 32 1193 32 2293 32 1481 32 2397
Fragments arranged by size:
2397 2293 1481 1193 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2157 2983 4083 4158 4324 2157
Size: 826 1100 75 166 5325
Fragments arranged by size:
5325 1100 826 166 75
PshAI GACnn'nnGTC
Cuts at: 1613 2720 1613
Size: 1107 6385
PsiI TTA'TAA
Cuts at: 5062 5062
Size: 7492
PspOMI G'GGCC_C
Cuts at: 2503 2917 2923 2503
Size: 414 6 7072
Fragments arranged by size:
7072 414 6
PstI C_TGCA'G
Cuts at: 830 4443 4844 830
Size: 3613 401 3478
Fragments arranged by size:
3613 3478 401
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7492
PvuII CAG'CTG
Cuts at: 3022 3022
Size: 7492
SalI G'TCGA_C
Cuts at: 1936 4834 1936
Size: 2898 4594
SanDI GG'GwC_CC
Cuts at: 4083 4158 4083
Size: 75 7417
SapI GCTCTTCn'nnn_
Cuts at: 2780 2849 3059 4235 4610 4721 2780
Size: 69 210 1176 375 111 5551
Fragments arranged by size:
5551 1176 375 210 111 69
SbfI CC_TGCA'GG
Cuts at: 4844 4844
Size: 7492
ScaI AGT'ACT
Cuts at: 4256 5740 7452 4256
Size: 1484 1712 4296
Fragments arranged by size:
4296 1712 1484
SfcI C'TryA_G
Cuts at: 826 1045 4439 4840 5975 6656 6847 826
Size: 219 3394 401 1135 681 191 1471
Fragments arranged by size:
3394 1471 1135 681 401 219 191
SfoI GGC'GCC
Cuts at: 2836 2887 3112 3162 3199 3546 4652 2836
Size: 51 225 50 37 347 1106 5676
Fragments arranged by size:
5676 1106 347 225 51 50 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7492
SmaI CCC'GGG
Cuts at: 1872 2922 4163 4819 1872
Size: 1050 1241 656 4545
Fragments arranged by size:
4545 1241 1050 656
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2978 2987 3872 4358 5596 6497
Size: 197 926 1035 9 885 486 1238 901
Cuts at: 6497 6759 820
Size: 262 1553
Fragments arranged by size:
1553 1238 1035 926 901 885 486 262
197 9
SnaBI TAC'GTA
Cuts at: 493 4789 5283 493
Size: 4296 494 2702
Fragments arranged by size:
4296 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7492
SphI G_CATG'C
Cuts at: 1132 4850 1132
Size: 3718 3774
SrfI GCCC'GGGC
Cuts at: 2922 2922
Size: 7492
SspI AAT'ATT
Cuts at: 5 52 5416 5
Size: 47 5364 2081
Fragments arranged by size:
5364 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7492
StyI C'CwwG_G
Cuts at: 513 1065 2022 2037 2256 2352 3336 3528 4071
Size: 552 957 15 219 96 984 192 543
Cuts at: 4071 4504 4931 513
Size: 433 427 3074
Fragments arranged by size:
3074 984 957 552 543 433 427 219
192 96 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 5519 5536 5689 5874 6319 325
Size: 1255 627 3312 17 153 185 445 1498
Fragments arranged by size:
3312 1498 1255 627 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2790 3357
Size: 274 80 33 51 1443 267 546 567
Cuts at: 3357 4254 4539 4737 5738 7450 96
Size: 897 285 198 1001 1712 138
Fragments arranged by size:
1712 1443 1001 897 567 546 285 274
267 198 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2433 2465 7452 7484 2433
Size: 32 4987 32 2441
Fragments arranged by size:
4987 2441 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2404 4367 1316
Size: 1088 1963 4441
Fragments arranged by size:
4441 1963 1088
XbaI T'CTAG_A
Cuts at: 4828 4828
Size: 7492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 3535 2029
Size: 1506 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7492
XmaI C'CCGG_G
Cuts at: 1870 2920 4161 4817 1870
Size: 1050 1241 656 4545
Fragments arranged by size:
4545 1241 1050 656
XmnI GAAnn'nnTTC
Cuts at: 4499 5621 4499
Size: 1122 6370
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5300 276
Size: 53 83 186 936 3766 2468
Fragments arranged by size:
3766 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI NruI NsiI
PacI PspXI PsrI RsrII SacI SacII SexAI SfiI
SwaI