Restriction map for
pFN21ASDA1087
(Linear) (Six-base) MAPSORT of: KIAA1087.seq Check: 2259 from: 1 to: 2763
KIAA1087.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 239 2763
Size: 239 2524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1756 2763
Size: 1756 1007
AfeI AGC'GCT
Cuts at: 0 504 775 2763
Size: 504 271 1988
Fragments arranged by size:
1988 504 271
AflII C'TTAA_G
Cuts at: 0 1849 2763
Size: 1849 914
AflIII A'CryG_T
Cuts at: 0 242 515 764 1724 2390 2435 2474 2763
Size: 242 273 249 960 666 45 39 289
Fragments arranged by size:
960 666 289 273 249 242 45 39
AhdI GACnn_n'nnGTC
Cuts at: 0 186 2763
Size: 186 2577
AleI CACnn'nnGTG
Cuts at: 0 493 856 1351 2763
Size: 493 363 495 1412
Fragments arranged by size:
1412 495 493 363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2629 2763
Size: 2629 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 838 870 1213 1245 2763
Size: 838 32 343 32 1518
Fragments arranged by size:
1518 838 343 32 32
ApaI G_GGCC'C
Cuts at: 0 484 898 904 2763
Size: 484 414 6 1859
Fragments arranged by size:
1859 484 414 6
ApaLI G'TGCA_C
Cuts at: 0 1711 2763
Size: 1711 1052
AvaI C'yCGr_G
Cuts at: 0 897 1644 2138 2763
Size: 897 747 494 625
Fragments arranged by size:
897 747 625 494
BanI G'GyrC_C
Cuts at: 0 355 487 811 862 1087 1137 1174 1521
Size: 355 132 324 51 225 50 37 347
Cuts at: 1521 1615 1657 2374 2627 2763
Size: 94 42 717 253 136
Fragments arranged by size:
717 355 347 324 253 225 136 132
94 51 50 42 37
BanII G_rGCy'C
Cuts at: 0 179 412 484 898 904 1324 2763
Size: 179 233 72 414 6 420 1439
Fragments arranged by size:
1439 420 414 233 179 72 6
BbeI G_GCGC'C
Cuts at: 0 815 866 1091 1141 1178 1525 2631 2763
Size: 815 51 225 50 37 347 1106 132
Fragments arranged by size:
1106 815 347 225 132 51 50 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 582 693 1172 2352 2583 2763
Size: 582 111 479 1180 231 180
Fragments arranged by size:
1180 582 479 231 180 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1173 1207 2763
Size: 1173 34 1556
Fragments arranged by size:
1556 1173 34
BglI GCCn_nnn'nGGC
Cuts at: 0 733 1259 1509 1845 2763
Size: 733 526 250 336 918
Fragments arranged by size:
918 733 526 336 250
Bme1580I G_kGCm'C
Cuts at: 0 358 484 898 904 1348 1618 1715 2222
Size: 358 126 414 6 444 270 97 507
Cuts at: 2222 2377 2763
Size: 155 386
Fragments arranged by size:
507 444 414 386 358 270 155 126
97 6
BmgBI CAC'GTC
Cuts at: 0 302 1626 2763
Size: 302 1324 1137
Fragments arranged by size:
1324 1137 302
BmrI ACTGGGnnnn_n'
Cuts at: 0 1836 2529 2763
Size: 1836 693 234
Fragments arranged by size:
1836 693 234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1323 2763
Size: 1323 1440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1237 1759 1996 2755 2763
Size: 1012 225 522 237 759 8
Fragments arranged by size:
1012 759 522 237 225 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 949 2320 2763
Size: 940 9 1371 443
Fragments arranged by size:
1371 940 443 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 337 1750 2763
Size: 337 1413 1013
Fragments arranged by size:
1413 1013 337
BsaAI yAC'GTr
Cuts at: 0 765 1250 1284 1599 2172 2763
Size: 765 485 34 315 573 591
Fragments arranged by size:
765 591 573 485 315 34
BsaBI GATnn'nnATC
Cuts at: 0 322 2763
Size: 322 2441
BsaHI Gr'CG_yC
Cuts at: 0 812 863 926 1088 1121 1138 1175 1522
Size: 812 51 63 162 33 17 37 347
Cuts at: 1522 2492 2628 2763
Size: 970 136 135
Fragments arranged by size:
970 812 347 162 136 135 63 51
37 33 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1213 1243 2119 2149 2293 2323 2763
Size: 1213 30 876 30 144 30 440
Fragments arranged by size:
1213 876 440 144 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 402 1432 1927 1930 1933 2005 2131
Size: 22 380 1030 495 3 3 72 126
Cuts at: 2131 2134 2137 2140 2158 2763
Size: 3 3 3 18 605
Fragments arranged by size:
1030 605 495 380 126 72 22 18
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 192 477 552 882 1830 2243 2652 2763
Size: 192 285 75 330 948 413 409 111
Fragments arranged by size:
948 413 409 330 285 192 111 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1579 2307 2548 2763
Size: 1015 564 728 241 215
Fragments arranged by size:
1015 728 564 241 215
BsiEI CG_ry'CG
Cuts at: 0 273 1145 2635 2763
Size: 273 872 1490 128
Fragments arranged by size:
1490 872 273 128
BsiHKAI G_wGCw'C
Cuts at: 0 1715 2207 2763
Size: 1715 492 556
Fragments arranged by size:
1715 556 492
BsmBI CGTCTCn'nnnn_
Cuts at: 0 370 1363 2763
Size: 370 993 1400
Fragments arranged by size:
1400 993 370
Bsp1286I G_dGCh'C
Cuts at: 0 179 358 412 484 898 904 1324 1348
Size: 179 179 54 72 414 6 420 24
Cuts at: 1348 1618 1715 2207 2222 2377 2763
Size: 270 97 492 15 155 386
Fragments arranged by size:
492 420 414 386 270 179 179 155
97 72 54 24 15 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1460 2763
Size: 1460 1303
BsrBI CCG'CTC
Cuts at: 0 2148 2763
Size: 2148 615
BsrDI GCAATG_nn'
Cuts at: 0 2606 2763
Size: 2606 157
BsrFI r'CCGG_y
Cuts at: 0 556 1084 1140 1970 2455 2624 2630 2763
Size: 556 528 56 830 485 169 6 133
Fragments arranged by size:
830 556 528 485 169 133 56 6
BsrGI T'GTAC_A
Cuts at: 0 767 2714 2763
Size: 767 1947 49
Fragments arranged by size:
1947 767 49
BssHII G'CGCG_C
Cuts at: 0 913 1649 1651 1696 2763
Size: 913 736 2 45 1067
Fragments arranged by size:
1067 913 736 45 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2406 2763
Size: 2406 357
BstYI r'GATC_y
Cuts at: 0 425 950 987 2763
Size: 425 525 37 1776
Fragments arranged by size:
1776 525 425 37
BtgI C'CryG_G
Cuts at: 0 233 1619 2238 2763
Size: 233 1386 619 525
Fragments arranged by size:
1386 619 525 233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 249 513 993 2763
Size: 249 264 480 1770
Fragments arranged by size:
1770 480 264 249
BtsI GCAGTG_nn'
Cuts at: 0 221 599 1209 2763
Size: 221 378 610 1554
Fragments arranged by size:
1554 610 378 221
DrdI GACnn_nn'nnGTC
Cuts at: 0 1630 2763
Size: 1630 1133
EaeI y'GGCC_r
Cuts at: 0 95 230 284 453 734 1142 1532 2507
Size: 95 135 54 169 281 408 390 975
Cuts at: 2507 2632 2763
Size: 125 131
Fragments arranged by size:
975 408 390 281 169 135 131 125
95 54
EagI C'GGCC_G
Cuts at: 0 1142 2632 2763
Size: 1142 1490 131
Fragments arranged by size:
1490 1142 131
EarI CTCTTCn'nnn_
Cuts at: 0 757 826 1036 1775 2212 2587 2698 2728
Size: 757 69 210 739 437 375 111 30
Cuts at: 2728 2763
Size: 35
Fragments arranged by size:
757 739 437 375 210 111 69 35
30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1132 2763
Size: 1132 1631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1237 1756 1759 1996 2755 2763
Size: 1012 225 519 3 237 759 8
Fragments arranged by size:
1012 759 519 237 225 8 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1128 2763
Size: 1128 1635
EcoO109I rG'GnC_Cy
Cuts at: 0 134 870 901 960 2060 2135 2301 2763
Size: 134 736 31 59 1100 75 166 462
Fragments arranged by size:
1100 736 462 166 134 75 59 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2223 2763
Size: 2223 540
FspI TGC'GCA
Cuts at: 0 1105 1387 1639 2551 2763
Size: 1105 282 252 912 212
Fragments arranged by size:
1105 912 282 252 212
FspAI rTGC'GCAy
Cuts at: 0 2551 2763
Size: 2551 212
HaeII r_GCGC'y
Cuts at: 0 506 688 777 815 866 1091 1141 1178
Size: 506 182 89 38 51 225 50 37
Cuts at: 1178 1525 2416 2631 2763
Size: 347 891 215 132
Fragments arranged by size:
891 506 347 225 215 182 132 89
51 50 38 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 245 277 367 399 1851 1883
Size: 172 32 41 32 90 32 1452 32
Cuts at: 1883 2119 2151 2293 2325 2660 2692 2763
Size: 236 32 142 32 335 32 71
Fragments arranged by size:
1452 335 236 172 142 90 71 41
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 811 862 1087 1137 1174 1521 2627 2763
Size: 811 51 225 50 37 347 1106 136
Fragments arranged by size:
1106 811 347 225 136 51 50 37
MluI A'CGCG_T
Cuts at: 0 1724 2435 2763
Size: 1724 711 328
Fragments arranged by size:
1724 711 328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1349 2486 2763
Size: 410 939 1137 277
Fragments arranged by size:
1137 939 410 277
MscI TGG'CCA
Cuts at: 0 232 1534 2763
Size: 232 1302 1229
Fragments arranged by size:
1302 1229 232
MslI CAynn'nnrTG
Cuts at: 0 397 493 520 532 856 1351 2611 2763
Size: 397 96 27 12 324 495 1260 152
Fragments arranged by size:
1260 495 397 324 152 96 27 12
MspA1I CmG'CkG
Cuts at: 0 143 797 999 1527 2763
Size: 143 654 202 528 1236
Fragments arranged by size:
1236 654 528 202 143
NaeI GCC'GGC
Cuts at: 0 558 1142 1972 2632 2763
Size: 558 584 830 660 131
Fragments arranged by size:
830 660 584 558 131
NarI GG'CG_CC
Cuts at: 0 812 863 1088 1138 1175 1522 2628 2763
Size: 812 51 225 50 37 347 1106 135
Fragments arranged by size:
1106 812 347 225 135 51 50 37
NcoI C'CATG_G
Cuts at: 0 233 2763
Size: 233 2530
NdeI CA'TA_TG
Cuts at: 0 1998 2763
Size: 1998 765
NgoMIV G'CCGG_C
Cuts at: 0 556 1140 1970 2630 2763
Size: 556 584 830 660 133
Fragments arranged by size:
830 660 584 556 133
NspI r_CATG'y
Cuts at: 0 246 519 1479 2763
Size: 246 273 960 1284
Fragments arranged by size:
1284 960 273 246
PasI CC'CwG_GG
Cuts at: 0 405 2370 2763
Size: 405 1965 393
Fragments arranged by size:
1965 405 393
PciI A'CATG_T
Cuts at: 0 242 515 2763
Size: 242 273 2248
Fragments arranged by size:
2248 273 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 1215 1957 2763
Size: 1215 742 806
Fragments arranged by size:
1215 806 742
PfoI T'CCnGG_A
Cuts at: 0 1547 1580 2763
Size: 1547 33 1183
Fragments arranged by size:
1547 1183 33
PmlI CAC'GTG
Cuts at: 0 1250 1599 2763
Size: 1250 349 1164
Fragments arranged by size:
1250 1164 349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1213 1245 2763
Size: 1213 32 1518
Fragments arranged by size:
1518 1213 32
PpuMI rG'GwC_Cy
Cuts at: 0 134 960 2060 2135 2301 2763
Size: 134 826 1100 75 166 462
Fragments arranged by size:
1100 826 462 166 134 75
PshAI GACnn'nnGTC
Cuts at: 0 697 2763
Size: 697 2066
PspOMI G'GGCC_C
Cuts at: 0 480 894 900 2763
Size: 480 414 6 1863
Fragments arranged by size:
1863 480 414 6
PstI C_TGCA'G
Cuts at: 0 2420 2763
Size: 2420 343
PvuII CAG'CTG
Cuts at: 0 999 2763
Size: 999 1764
SanDI GG'GwC_CC
Cuts at: 0 2060 2135 2763
Size: 2060 75 628
Fragments arranged by size:
2060 628 75
SapI GCTCTTCn'nnn_
Cuts at: 0 757 826 1036 2212 2587 2698 2763
Size: 757 69 210 1176 375 111 65
Fragments arranged by size:
1176 757 375 210 111 69 65
ScaI AGT'ACT
Cuts at: 0 2233 2763
Size: 2233 530
SfcI C'TryA_G
Cuts at: 0 2416 2763
Size: 2416 347
SfoI GGC'GCC
Cuts at: 0 813 864 1089 1139 1176 1523 2629 2763
Size: 813 51 225 50 37 347 1106 134
Fragments arranged by size:
1106 813 347 225 134 51 50 37
SmaI CCC'GGG
Cuts at: 0 899 2140 2763
Size: 899 1241 623
Fragments arranged by size:
1241 899 623
SmlI C'TyrA_G
Cuts at: 0 955 964 1849 2335 2763
Size: 955 9 885 486 428
Fragments arranged by size:
955 885 486 428 9
SrfI GCCC'GGGC
Cuts at: 0 899 2763
Size: 899 1864
StyI C'CwwG_G
Cuts at: 0 14 233 329 1313 1505 2048 2481 2763
Size: 14 219 96 984 192 543 433 282
Fragments arranged by size:
984 543 433 282 219 192 96 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 2763
Size: 184 2579
TatI w'GTAC_w
Cuts at: 0 221 767 1334 2231 2516 2714 2763
Size: 221 546 567 897 285 198 49
Fragments arranged by size:
897 567 546 285 221 198 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 410 442 2763
Size: 410 32 2321
Fragments arranged by size:
2321 410 32
Tth111I GACn'n_nGTC
Cuts at: 0 381 2344 2763
Size: 381 1963 419
Fragments arranged by size:
1963 419 381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1512 2763
Size: 1512 1251
XmaI C'CCGG_G
Cuts at: 0 897 2138 2763
Size: 897 1241 625
Fragments arranged by size:
1241 897 625
XmnI GAAnn'nnTTC
Cuts at: 0 2476 2763
Size: 2476 287
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AhdI AleI AlfI
AloI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BcgI BglI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstXI BstYI BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
FspI FspAI HaeII Hin4I KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuII SanDI SapI ScaI SfcI SfoI
SmaI SmlI SrfI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AlwNI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglII BlpI BmtI Bpu10I BsaWI BsiWI BsmI
BspEI BspHI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII EcoICRI EcoRI EcoRV FseI
HincII HindIII HpaI KpnI MfeI NheI NotI NruI
NsiI PacI PmeI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI
ZraI