(Circular) (Six-base) MAPSORT of: pFN21ASDA1066.seq Check: 5502 from: 1 to: 8737 pFN21ASDA1066 8737 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6001 6001 Size: 8737 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6547 278 Size: 53 83 186 936 5011 2468 Fragments arranged by size: 5011 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 3366 3731 6080 1937 Size: 1245 184 365 2349 4594 Fragments arranged by size: 4594 2349 1245 365 184 Acc65I G'GTAC_C Cuts at: 6058 6058 Size: 8737 AclI AA'CG_TT Cuts at: 1563 6864 7237 1563 Size: 5301 373 3063 Fragments arranged by size: 5301 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 3733 3745 3829 5036 6800 8184 8452 Size: 824 1277 12 84 1207 1764 1384 268 Cuts at: 8452 1632 Size: 1917 Fragments arranged by size: 1917 1764 1384 1277 1207 824 268 84 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8540 AflIII A'CryG_T Cuts at: 1203 2649 3078 4278 5637 7636 8613 1203 Size: 1446 429 1200 1359 1999 977 1327 Fragments arranged by size: 1999 1446 1359 1327 1200 977 429 AgeI A'CCGG_T Cuts at: 7568 7568 Size: 8737 AhdI GACnn_n'nnGTC Cuts at: 3034 3373 3925 7466 3034 Size: 339 552 3541 4305 Fragments arranged by size: 4305 3541 552 339 AleI CACnn'nnGTG Cuts at: 1166 2727 3596 4409 4565 4628 4721 5100 1166 Size: 1561 869 813 156 63 93 379 4803 Fragments arranged by size: 4803 1561 869 813 379 156 93 63 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2717 5748 1184 Size: 1533 3031 4173 Fragments arranged by size: 4173 3031 1533 AlwNI CAG_nnn'CTG Cuts at: 2905 3194 3313 4733 4856 5543 5569 5857 6480 Size: 289 119 1420 123 687 26 288 623 Cuts at: 6480 8052 2905 Size: 1572 3590 Fragments arranged by size: 3590 1572 1420 687 623 289 288 123 119 26 ApaI G_GGCC'C Cuts at: 4740 5816 4740 Size: 1076 7661 ApaLI G'TGCA_C Cuts at: 2679 4967 5222 6794 7950 2679 Size: 2288 255 1572 1156 3466 Fragments arranged by size: 3466 2288 1572 1156 255 ApoI r'AATT_y Cuts at: 1493 2342 3902 6046 6272 6424 8533 1493 Size: 849 1560 2144 226 152 2109 1697 Fragments arranged by size: 2144 2109 1697 1560 849 226 152 AseI AT'TA_AT Cuts at: 160 1032 7291 160 Size: 872 6259 1606 Fragments arranged by size: 6259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8737 AvaI C'yCGr_G Cuts at: 1870 1943 2946 3696 4286 4490 4531 4614 4769 Size: 73 1003 750 590 204 41 83 155 Cuts at: 4769 5621 6026 6062 1870 Size: 852 405 36 4545 Fragments arranged by size: 4545 1003 852 750 590 405 204 155 83 73 41 36 AvrII C'CTAG_G Cuts at: 3232 3232 Size: 8737 BamHI G'GATC_C Cuts at: 4344 6067 6434 4344 Size: 1723 367 6647 Fragments arranged by size: 6647 1723 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2721 2831 4093 4723 5501 Size: 325 217 627 934 110 1262 630 778 Cuts at: 5501 5612 5724 5755 6058 7513 618 Size: 111 112 31 303 1455 1842 Fragments arranged by size: 1842 1455 1262 934 778 630 627 325 303 217 112 111 110 31 BanII G_rGCy'C Cuts at: 1391 2126 2187 2927 4266 4311 4740 4890 4948 Size: 735 61 740 1339 45 429 150 58 Cuts at: 4948 5409 5577 5816 6056 1391 Size: 461 168 239 240 4072 Fragments arranged by size: 4072 1339 740 735 461 429 240 239 168 150 61 58 45 BbeI G_GCGC'C Cuts at: 5616 5616 Size: 8737 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4783 928 Size: 971 2884 4882 Fragments arranged by size: 4882 2884 971 BbvCI CC'TCA_GC Cuts at: 3395 3395 Size: 8737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6928 6962 1903 Size: 34 4991 34 3678 Fragments arranged by size: 4991 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6629 7839 6629 Size: 1210 7527 BclI T'GATC_A Cuts at: 1548 3684 4380 1548 Size: 2136 696 5905 Fragments arranged by size: 5905 2136 696 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2121 4712 7348 Size: 107 122 71 631 605 449 2591 2636 Cuts at: 7348 136 Size: 1525 Fragments arranged by size: 2636 2591 1525 631 605 449 122 107 71 BglII A'GATC_T Cuts at: 8732 8732 Size: 8737 BlpI GC'TnA_GC Cuts at: 2231 3417 5030 5876 6154 2231 Size: 1186 1613 846 278 4814 Fragments arranged by size: 4814 1613 1186 846 278 Bme1580I G_kGCm'C Cuts at: 1790 2683 2865 2947 3875 4406 4740 4971 5226 Size: 893 182 82 928 531 334 231 255 Cuts at: 5226 5504 5727 5816 6798 7954 1790 Size: 278 223 89 982 1156 2573 Fragments arranged by size: 2573 1156 982 928 893 531 334 278 255 231 223 182 89 82 BmgBI CAC'GTC Cuts at: 1318 3927 4281 4516 4789 5227 5395 1318 Size: 2609 354 235 273 438 168 4660 Fragments arranged by size: 4660 2609 438 354 273 235 168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2293 4622 4684 Size: 416 53 83 399 24 876 2329 62 Cuts at: 4684 6468 7426 442 Size: 1784 958 1753 Fragments arranged by size: 2329 1784 1753 958 876 416 399 83 62 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8737 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5582 5582 Size: 8737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2282 2450 2740 3757 4039 4315 4438 Size: 992 51 168 290 1017 282 276 123 Cuts at: 4438 7397 1239 Size: 2959 2579 Fragments arranged by size: 2959 2579 1017 992 290 282 276 168 123 51 Bpu10I CC'TnA_GC Cuts at: 3395 4166 3395 Size: 771 7966 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6862 7727 8025 6862 Size: 865 298 7574 Fragments arranged by size: 7574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3083 7400 882 Size: 617 1584 4317 2219 Fragments arranged by size: 4317 2219 1584 617 BsaAI yAC'GTr Cuts at: 493 1204 2548 2678 3982 4441 5638 6034 6528 Size: 711 1344 130 1304 459 1197 396 494 Cuts at: 6528 493 Size: 2702 Fragments arranged by size: 2702 1344 1304 1197 711 494 459 396 130 BsaBI GATnn'nnATC Cuts at: 2030 8731 2030 Size: 6701 2036 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4158 4323 4389 5268 Size: 53 83 186 936 2625 165 66 879 Cuts at: 5268 5613 6544 6926 7561 275 Size: 345 931 382 635 1451 Fragments arranged by size: 2625 1451 936 931 879 635 382 345 186 165 83 66 53 BsaWI w'CCGG_w Cuts at: 7170 7568 7842 7989 8400 7170 Size: 398 274 147 411 7507 Fragments arranged by size: 7507 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2093 2096 2123 2468 Size: 30 165 30 663 27 3 27 345 Cuts at: 2468 2498 3146 3176 4313 4343 4595 4625 1178 Size: 30 648 30 1137 30 252 30 5290 Fragments arranged by size: 5290 1137 663 648 345 252 165 30 30 30 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2351 2489 4600 4715 5951 1186 Size: 997 168 138 2111 115 1236 3972 Fragments arranged by size: 3972 2111 1236 997 168 138 115 BseYI C'CCAG_C Cuts at: 2819 3576 4196 4739 4801 4827 4889 4939 5440 Size: 757 620 543 62 26 62 50 501 Cuts at: 5440 5706 5803 7940 8436 2819 Size: 266 97 2137 496 3120 Fragments arranged by size: 3120 2137 757 620 543 501 496 266 97 62 62 50 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 3907 4908 4985 5537 1163 Size: 573 762 1409 1001 77 552 4363 Fragments arranged by size: 4363 1409 1001 762 573 552 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2811 2871 4000 4246 5194 6187 Size: 275 186 810 60 1129 246 948 993 Cuts at: 6187 6948 7976 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1129 1028 993 948 810 761 275 246 186 60 BsiHKAI G_wGCw'C Cuts at: 2187 2232 2250 2406 2556 2683 3618 3705 3981 Size: 45 18 156 150 127 935 87 276 Cuts at: 3981 4311 4788 4971 5226 6798 6883 7954 2187 Size: 330 477 183 255 1572 85 1071 2970 Fragments arranged by size: 2970 1572 1071 935 477 330 276 255 183 156 150 127 87 85 45 18 BsiWI C'GTAC_G Cuts at: 5455 5455 Size: 8737 BsmI GAATG_Cn' Cuts at: 6248 6341 6248 Size: 93 8644 BsmBI CGTCTCn'nnnn_ Cuts at: 4248 4287 5907 5924 4248 Size: 39 1620 17 7061 Fragments arranged by size: 7061 1620 39 17 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2187 2232 2250 2406 2556 2683 Size: 399 336 61 45 18 156 150 127 Cuts at: 2683 2865 2927 2947 3618 3705 3875 3981 4266 Size: 182 62 20 671 87 170 106 285 Cuts at: 4266 4311 4406 4740 4788 4890 4948 4971 5226 Size: 45 95 334 48 102 58 23 255 Cuts at: 5226 5409 5504 5577 5727 5816 6056 6798 6883 Size: 183 95 73 150 89 240 742 85 Cuts at: 6883 7954 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 671 399 336 334 285 255 240 183 182 170 156 150 150 127 106 102 95 95 89 87 85 73 62 61 58 48 45 45 23 20 18 BspEI T'CCGG_A Cuts at: 8400 8400 Size: 8737 BspHI T'CATG_A Cuts at: 6626 8356 6626 Size: 1730 7007 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2569 4247 6001 6092 844 Size: 1725 1678 1754 91 3489 Fragments arranged by size: 3489 1754 1725 1678 91 BsrBI CCG'CTC Cuts at: 1394 1823 2104 2479 3625 3643 4170 5880 6624 Size: 429 281 375 1146 18 527 1710 744 Cuts at: 6624 1394 Size: 3507 Fragments arranged by size: 3507 1710 1146 744 527 429 375 281 18 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 7232 7406 65 Size: 1183 3512 2472 174 1396 Fragments arranged by size: 3512 2472 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3839 3891 4090 4096 4553 5487 5504 Size: 110 2029 52 199 6 457 934 17 Cuts at: 5504 7381 7568 1700 Size: 1877 187 2869 Fragments arranged by size: 2869 2029 1877 934 457 199 187 110 52 17 6 BsrGI T'GTAC_A Cuts at: 96 3548 96 Size: 3452 5285 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8737 BssSI C'ACGA_G Cuts at: 2249 2699 6797 7809 2249 Size: 450 4098 1012 3177 Fragments arranged by size: 4098 3177 1012 450 BstAPI GCAn_nnn'nTGC Cuts at: 794 3391 794 Size: 2597 6140 BstBI TT'CG_AA Cuts at: 2007 7541 2007 Size: 5534 3203 BstEII G'GTnAC_C Cuts at: 1184 2983 1184 Size: 1799 6938 BstXI CCAn_nnnn'nTGG Cuts at: 4375 4939 5341 5642 4375 Size: 564 402 301 7470 Fragments arranged by size: 7470 564 402 301 BstYI r'GATC_y Cuts at: 1972 2031 4344 5119 6067 6434 6819 6836 8288 Size: 59 2313 775 948 367 385 17 1452 Cuts at: 8288 8374 8386 8732 1972 Size: 86 12 346 1977 Fragments arranged by size: 2313 1977 1452 948 775 385 367 346 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 5354 5354 Size: 8737 BtgI C'CryG_G Cuts at: 513 1065 3570 4152 4464 4595 4716 4902 5098 Size: 552 2505 582 312 131 121 186 196 Cuts at: 5098 5276 5694 5719 513 Size: 178 418 25 3531 Fragments arranged by size: 3531 2505 582 552 418 312 196 186 178 131 121 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3267 3481 4648 5331 5661 5745 Size: 683 56 2041 214 1167 683 330 84 Cuts at: 5745 487 Size: 3479 Fragments arranged by size: 3479 2041 1167 683 683 330 214 84 56 BtsI GCAGTG_nn' Cuts at: 2155 3031 3834 4924 5662 5794 6253 7047 7067 Size: 876 803 1090 738 132 459 794 20 Cuts at: 7067 2155 Size: 3825 Fragments arranged by size: 3825 1090 876 803 794 738 459 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8737 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8702 DraI TTT'AAA Cuts at: 6041 6041 Size: 8737 DraIII CAC_nnn'GTG Cuts at: 2254 4935 2254 Size: 2681 6056 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3190 7744 809 Size: 507 1874 4554 1802 Fragments arranged by size: 4554 1874 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2737 2808 2868 Size: 54 1425 50 275 18 907 71 60 Cuts at: 2868 3786 3997 4467 4551 4656 4874 4980 5085 Size: 918 211 470 84 105 218 106 105 Cuts at: 5085 5110 5667 5772 6184 7073 8702 8 Size: 25 557 105 412 889 1629 43 Fragments arranged by size: 1629 1425 918 907 889 557 470 412 275 218 211 106 105 105 105 84 71 60 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2808 2868 3997 6184 1537 Size: 275 996 60 1129 2187 4090 Fragments arranged by size: 4090 2187 1129 996 275 60 EarI CTCTTCn'nnn_ Cuts at: 1353 3456 3513 3764 3772 6667 1353 Size: 2103 57 251 8 2895 3423 Fragments arranged by size: 3423 2895 2103 251 57 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2348 2635 3539 3922 5087 5327 Size: 1064 138 759 287 904 383 1165 240 Cuts at: 5327 7324 7698 7844 387 Size: 1997 374 146 1280 Fragments arranged by size: 1997 1280 1165 1064 904 759 383 374 287 240 146 138 EcoICRI GAG'CTC Cuts at: 2185 4309 2185 Size: 2124 6613 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2282 2450 2456 2740 3733 3745 Size: 393 599 51 168 6 284 993 12 Cuts at: 3745 3757 3829 4039 4315 4438 5036 6800 7397 Size: 12 72 210 276 123 598 1764 597 Cuts at: 7397 8184 8452 1239 Size: 787 268 1524 Fragments arranged by size: 1764 1524 993 787 599 598 597 393 284 276 268 210 168 123 72 51 12 12 6 EcoO109I rG'GnC_Cy Cuts at: 4126 4528 4897 5079 5812 5813 4126 Size: 402 369 182 733 1 7050 Fragments arranged by size: 7050 733 402 369 182 1 EcoRI G'AATT_C Cuts at: 3902 6046 6424 3902 Size: 2144 378 6215 Fragments arranged by size: 6215 2144 378 FspI TGC'GCA Cuts at: 2324 2999 7243 2324 Size: 675 4244 3818 Fragments arranged by size: 4244 3818 675 HaeII r_GCGC'y Cuts at: 1678 2149 2321 2635 3583 3796 3856 4220 4275 Size: 471 172 314 948 213 60 364 55 Cuts at: 4275 5616 7884 1678 Size: 1341 2268 2531 Fragments arranged by size: 2531 2268 1341 948 471 364 314 213 172 60 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2093 2098 2125 2468 Size: 32 135 32 494 27 5 27 343 Cuts at: 2468 2500 2768 2800 3020 3052 3359 3391 3692 Size: 32 268 32 220 32 307 32 301 Cuts at: 3692 3724 3911 3943 4313 4345 5116 5148 5505 Size: 32 187 32 370 32 771 32 357 Cuts at: 5505 5537 7378 7410 7452 7484 1373 Size: 32 1841 32 42 32 2626 Fragments arranged by size: 2626 1841 771 494 370 357 343 307 301 268 220 187 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3367 3732 4162 4681 6081 Size: 988 281 1245 184 365 430 519 1400 Cuts at: 6081 6327 669 Size: 246 3079 Fragments arranged by size: 3079 1400 1245 988 519 430 365 281 246 184 HindIII A'AGCT_T Cuts at: 748 3141 748 Size: 2393 6344 HpaI GTT'AAC Cuts at: 6327 6327 Size: 8737 KasI G'GCGC_C Cuts at: 5612 5612 Size: 8737 KpnI G_GTAC'C Cuts at: 6062 6062 Size: 8737 MfeI C'AATT_G Cuts at: 6336 6336 Size: 8737 MluI A'CGCG_T Cuts at: 8613 8613 Size: 8737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 2451 2466 2563 2865 3387 7851 8035 Size: 774 372 15 97 302 522 4464 184 Cuts at: 8035 1305 Size: 2007 Fragments arranged by size: 4464 2007 774 522 372 302 184 97 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 4658 4982 5087 5112 5774 Size: 54 1425 343 2826 324 105 25 662 Cuts at: 5774 10 Size: 2973 Fragments arranged by size: 2973 2826 1425 662 343 324 105 54 25 MslI CAynn'nnrTG Cuts at: 518 1166 2531 2727 3596 4076 4409 4565 4628 Size: 648 1365 196 869 480 333 156 63 Cuts at: 4628 4721 5100 5636 5640 5730 6002 6695 7054 Size: 93 379 536 4 90 272 693 359 Cuts at: 7054 7213 518 Size: 159 2042 Fragments arranged by size: 2042 1365 869 693 648 536 480 379 359 333 272 196 159 156 93 90 63 4 MspA1I CmG'CkG Cuts at: 1606 2194 2290 2428 2521 2743 3373 4045 4154 Size: 588 96 138 93 222 630 672 109 Cuts at: 4154 4466 4584 4597 4893 5172 5278 5302 5825 Size: 312 118 13 296 279 106 24 523 Cuts at: 5825 6153 6830 7978 8223 1606 Size: 328 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 630 588 523 328 312 296 279 245 222 138 118 109 106 96 93 24 13 NaeI GCC'GGC Cuts at: 3841 3893 5489 3841 Size: 52 1596 7089 Fragments arranged by size: 7089 1596 52 NarI GG'CG_CC Cuts at: 5613 5613 Size: 8737 NcoI C'CATG_G Cuts at: 513 1065 3570 5694 5719 513 Size: 552 2505 2124 25 3531 Fragments arranged by size: 3531 2505 2124 552 25 NdeI CA'TA_TG Cuts at: 387 387 Size: 8737 NgoMIV G'CCGG_C Cuts at: 3839 3891 5487 3839 Size: 52 1596 7089 Fragments arranged by size: 7089 1596 52 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8737 NotI GC'GGCC_GC Cuts at: 2808 6184 2808 Size: 3376 5361 NspI r_CATG'y Cuts at: 1132 1229 3082 5045 6095 7640 1132 Size: 97 1853 1963 1050 1545 2229 Fragments arranged by size: 2229 1963 1853 1545 1050 97 PasI CC'CwG_GG Cuts at: 1348 2905 3124 4733 5816 1348 Size: 1557 219 1609 1083 4269 Fragments arranged by size: 4269 1609 1557 1083 219 PciI A'CATG_T Cuts at: 3078 7636 3078 Size: 4558 4179 PflMI CCAn_nnn'nTGG Cuts at: 1108 4733 4935 5569 1108 Size: 3625 202 634 4276 Fragments arranged by size: 4276 3625 634 202 PfoI T'CCnGG_A Cuts at: 3714 4503 3714 Size: 789 7948 PmeI GTTT'AAAC Cuts at: 6041 6041 Size: 8737 PmlI CAC'GTG Cuts at: 3982 4441 5638 3982 Size: 459 1197 7081 Fragments arranged by size: 7081 1197 459 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2207 2239 6806 6838 8319 8351 2207 Size: 32 4567 32 1481 32 2593 Fragments arranged by size: 4567 2593 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4126 4528 4897 5079 4126 Size: 402 369 182 7784 Fragments arranged by size: 7784 402 369 182 PshAI GACnn'nnGTC Cuts at: 1613 3081 3999 5546 1613 Size: 1468 918 1547 4804 Fragments arranged by size: 4804 1547 1468 918 PsiI TTA'TAA Cuts at: 6307 6307 Size: 8737 PspOMI G'GGCC_C Cuts at: 4736 5812 4736 Size: 1076 7661 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3803 3835 3803 Size: 32 8705 PstI C_TGCA'G Cuts at: 830 2748 2901 3660 4008 6089 830 Size: 1918 153 759 348 2081 3478 Fragments arranged by size: 3478 2081 1918 759 348 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8737 PvuII CAG'CTG Cuts at: 2290 2428 2743 3373 4045 5302 5825 2290 Size: 138 315 630 672 1257 523 5202 Fragments arranged by size: 5202 1257 672 630 523 315 138 SacI G_AGCT'C Cuts at: 2187 4311 2187 Size: 2124 6613 SacII CC_GC'GG Cuts at: 4155 4467 4598 5279 4155 Size: 312 131 681 7613 Fragments arranged by size: 7613 681 312 131 SalI G'TCGA_C Cuts at: 1936 3181 3365 3730 6079 1936 Size: 1245 184 365 2349 4594 Fragments arranged by size: 4594 2349 1245 365 184 SanDI GG'GwC_CC Cuts at: 4897 5079 4897 Size: 182 8555 SapI GCTCTTCn'nnn_ Cuts at: 3772 3772 Size: 8737 SbfI CC_TGCA'GG Cuts at: 6089 6089 Size: 8737 ScaI AGT'ACT Cuts at: 6985 8697 6985 Size: 1712 7025 SfcI C'TryA_G Cuts at: 826 1045 2744 2897 3656 4004 5387 6085 7220 Size: 219 1699 153 759 348 1383 698 1135 Cuts at: 7220 7901 8092 826 Size: 681 191 1471 Fragments arranged by size: 1699 1471 1383 1135 759 698 681 348 219 191 153 SfiI GGCCn_nnn'nGGCC Cuts at: 4712 4712 Size: 8737 SfoI GGC'GCC Cuts at: 5614 5614 Size: 8737 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8737 SmaI CCC'GGG Cuts at: 1872 2948 4492 4533 4771 5623 6064 1872 Size: 1076 1544 41 238 852 441 4545 Fragments arranged by size: 4545 1544 1076 852 441 238 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 6841 7742 8004 820 Size: 197 926 4898 901 262 1553 Fragments arranged by size: 4898 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6034 6528 493 Size: 5541 494 2702 Fragments arranged by size: 5541 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8737 SphI G_CATG'C Cuts at: 1132 6095 1132 Size: 4963 3774 SrfI GCCC'GGGC Cuts at: 4771 4771 Size: 8737 SspI AAT'ATT Cuts at: 5 52 6661 5 Size: 47 6609 2081 Fragments arranged by size: 6609 2081 47 StuI AGG'CCT Cuts at: 1517 4010 1517 Size: 2493 6244 StyI C'CwwG_G Cuts at: 513 1065 3232 3360 3570 4302 5694 5719 5979 Size: 552 2167 128 210 732 1392 25 260 Cuts at: 5979 6176 513 Size: 197 3074 Fragments arranged by size: 3074 2167 1392 732 552 260 210 197 128 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2312 2615 2833 3002 3720 3844 4260 Size: 1255 732 303 218 169 718 124 416 Cuts at: 4260 4942 5317 5485 6764 6781 6934 7119 7564 Size: 682 375 168 1279 17 153 185 445 Cuts at: 7564 325 Size: 1498 Fragments arranged by size: 1498 1279 1255 732 718 682 445 416 375 303 218 185 169 168 153 124 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2502 2977 3548 Size: 274 80 33 51 1443 525 475 571 Cuts at: 3548 3633 4035 4101 6983 8695 96 Size: 85 402 66 2882 1712 138 Fragments arranged by size: 2882 1712 1443 571 525 475 402 274 138 85 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8697 8729 8697 Size: 32 8705 Tth111I GACn'n_nGTC Cuts at: 1316 4813 1316 Size: 3497 5240 XbaI T'CTAG_A Cuts at: 6073 6073 Size: 8737 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3070 3433 4372 4936 5173 3070 Size: 363 939 564 237 6634 Fragments arranged by size: 6634 939 564 363 237 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8737 XmaI C'CCGG_G Cuts at: 1870 2946 4490 4531 4769 5621 6062 1870 Size: 1076 1544 41 238 852 441 4545 Fragments arranged by size: 4545 1544 1076 852 441 238 41 XmnI GAAnn'nnTTC Cuts at: 2651 6866 2651 Size: 4215 4522 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6545 276 Size: 53 83 186 936 5011 2468 Fragments arranged by size: 5011 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI RsrII SexAI SwaI