(Circular) (Six-base) MAPSORT of: pFN21ASDA1059.seq Check: 825 from: 1 to: 8395 pFN21ASDA1059 8395 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2317 3140 3684 4146 2317 Size: 823 544 462 6566 Fragments arranged by size: 6566 823 544 462 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6205 278 Size: 53 83 186 936 4669 2468 Fragments arranged by size: 4669 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2099 2720 5738 1937 Size: 162 621 3018 4594 Fragments arranged by size: 4594 3018 621 162 Acc65I G'GTAC_C Cuts at: 5716 5716 Size: 8395 AclI AA'CG_TT Cuts at: 1563 6522 6895 1563 Size: 4959 373 3063 Fragments arranged by size: 4959 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2577 4030 4081 4621 5169 5333 6458 7842 Size: 945 1453 51 540 548 164 1125 1384 Cuts at: 7842 8110 1632 Size: 268 1917 Fragments arranged by size: 1917 1453 1384 1125 945 548 540 268 164 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8198 AflIII A'CryG_T Cuts at: 1203 3264 3393 3419 4665 7294 8271 1203 Size: 2061 129 26 1246 2629 977 1327 Fragments arranged by size: 2629 2061 1327 1246 977 129 26 AgeI A'CCGG_T Cuts at: 7226 7226 Size: 8395 AhdI GACnn_n'nnGTC Cuts at: 2145 7124 2145 Size: 4979 3416 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8395 AlwNI CAG_nnn'CTG Cuts at: 2387 2920 6138 7710 2387 Size: 533 3218 1572 3072 Fragments arranged by size: 3218 3072 1572 533 ApaLI G'TGCA_C Cuts at: 2624 3086 3638 6452 7608 2624 Size: 462 552 2814 1156 3411 Fragments arranged by size: 3411 2814 1156 552 462 ApoI r'AATT_y Cuts at: 1493 5293 5704 5930 6082 8191 1493 Size: 3800 411 226 152 2109 1697 Fragments arranged by size: 3800 2109 1697 411 226 152 AseI AT'TA_AT Cuts at: 160 1032 6949 160 Size: 872 5917 1606 Fragments arranged by size: 5917 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8395 AvaI C'yCGr_G Cuts at: 1870 1943 2514 4485 5720 1870 Size: 73 571 1971 1235 4545 Fragments arranged by size: 4545 1971 1235 571 73 BamHI G'GATC_C Cuts at: 3138 5725 6092 3138 Size: 2587 367 5441 Fragments arranged by size: 5441 2587 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2117 2268 2530 2732 Size: 325 217 627 278 52 151 262 202 Cuts at: 2732 3594 3875 4134 5716 7171 618 Size: 862 281 259 1582 1455 1842 Fragments arranged by size: 1842 1582 1455 862 627 325 281 278 262 259 217 202 151 52 BanII G_rGCy'C Cuts at: 1391 2088 3774 3997 4207 5119 5714 1391 Size: 697 1686 223 210 912 595 4072 Fragments arranged by size: 4072 1686 912 697 595 223 210 BbeI G_GCGC'C Cuts at: 2069 2121 2272 2534 3598 2069 Size: 52 151 262 1064 6866 Fragments arranged by size: 6866 1064 262 151 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2565 2777 3007 3566 928 Size: 971 666 212 230 559 5757 Fragments arranged by size: 5757 971 666 559 230 212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2566 2600 6586 6620 1903 Size: 34 629 34 3986 34 3678 Fragments arranged by size: 3986 3678 629 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6287 7497 6287 Size: 1210 7185 BclI T'GATC_A Cuts at: 1548 2859 1548 Size: 1311 7084 BfrBI ATG'CAT Cuts at: 3019 3019 Size: 8395 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 3884 3913 Size: 107 122 71 631 605 380 1832 29 Cuts at: 3913 7006 136 Size: 3093 1525 Fragments arranged by size: 3093 1832 1525 631 605 380 122 107 71 29 BglII A'GATC_T Cuts at: 3831 8390 3831 Size: 4559 3836 BlpI GC'TnA_GC Cuts at: 5395 5812 5395 Size: 417 7978 Bme1580I G_kGCm'C Cuts at: 1790 2628 3090 3642 3880 4216 4472 4539 5388 Size: 838 462 552 238 336 256 67 849 Cuts at: 5388 6456 7612 1790 Size: 1068 1156 2573 Fragments arranged by size: 2573 1156 1068 849 838 552 462 336 256 238 67 BmgBI CAC'GTC Cuts at: 1318 2103 1318 Size: 785 7610 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2956 3629 3877 Size: 416 53 83 399 24 1539 673 248 Cuts at: 3877 4404 4833 4856 6126 7084 442 Size: 527 429 23 1270 958 1753 Fragments arranged by size: 1753 1539 1270 958 673 527 429 416 399 248 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8395 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3961 3961 Size: 8395 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5359 7055 1239 Size: 4120 1696 2579 Fragments arranged by size: 4120 2579 1696 Bpu10I CC'TnA_GC Cuts at: 2505 4436 2505 Size: 1931 6464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5478 6520 7385 7683 5478 Size: 1042 865 298 6190 Fragments arranged by size: 6190 1042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4010 4218 4249 5180 7058 882 Size: 617 2511 208 31 931 1878 2219 Fragments arranged by size: 2511 2219 1878 931 617 208 31 BsaAI yAC'GTr Cuts at: 493 1204 3265 3396 4666 5692 6186 493 Size: 711 2061 131 1270 1026 494 2702 Fragments arranged by size: 2702 2061 1270 1026 711 494 131 BsaBI GATnn'nnATC Cuts at: 5483 8389 5483 Size: 2906 5489 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2118 2155 2240 Size: 53 83 186 936 533 52 37 85 Cuts at: 2240 2269 2454 2531 3595 6202 6584 7219 275 Size: 29 185 77 1064 2607 382 635 1451 Fragments arranged by size: 2607 1451 1064 936 635 533 382 186 185 85 83 77 53 52 37 29 BsaWI w'CCGG_w Cuts at: 2076 3610 6828 7226 7500 7647 8058 2076 Size: 1534 3218 398 274 147 411 2413 Fragments arranged by size: 3218 2413 1534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 4301 4331 5344 Size: 30 165 30 822 30 2046 30 1013 Cuts at: 5344 5374 1178 Size: 30 4199 Fragments arranged by size: 4199 2046 1013 822 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2246 2422 2425 2534 3793 4580 5242 5603 Size: 1060 176 3 109 1259 787 662 361 Cuts at: 5603 1186 Size: 3978 Fragments arranged by size: 3978 1259 1060 787 662 361 176 109 3 BseYI C'CCAG_C Cuts at: 2263 2295 2380 2540 2573 4324 4970 5028 5381 Size: 32 85 160 33 1751 646 58 353 Cuts at: 5381 7598 8094 2263 Size: 2217 496 2564 Fragments arranged by size: 2564 2217 1751 646 496 353 160 85 58 33 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3656 4408 4845 5662 1163 Size: 573 1920 752 437 817 3896 Fragments arranged by size: 3896 1920 817 752 573 437 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2475 4509 5845 6606 7634 1540 Size: 275 186 474 2034 1336 761 1028 2301 Fragments arranged by size: 2301 2034 1336 1028 761 474 275 186 BsiHKAI G_wGCw'C Cuts at: 2628 3090 3642 3774 4207 5119 6456 6541 7612 Size: 462 552 132 433 912 1337 85 1071 Cuts at: 7612 2628 Size: 3411 Fragments arranged by size: 3411 1337 1071 912 552 462 433 132 85 BsmI GAATG_Cn' Cuts at: 4981 5906 5999 4981 Size: 925 93 7377 Fragments arranged by size: 7377 925 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2628 3090 3642 3774 3880 3997 Size: 399 298 540 462 552 132 106 117 Cuts at: 3997 4207 4216 4472 4539 5119 5388 5714 6456 Size: 210 9 256 67 580 269 326 742 Cuts at: 6456 6541 7612 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 580 552 540 462 399 326 298 269 256 210 132 117 106 85 67 9 BspEI T'CCGG_A Cuts at: 2076 3610 8058 2076 Size: 1534 4448 2413 Fragments arranged by size: 4448 2413 1534 BspHI T'CATG_A Cuts at: 4026 5011 6284 8014 4026 Size: 985 1273 1730 4407 Fragments arranged by size: 4407 1730 1273 985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2317 3140 3500 3684 4146 5750 844 Size: 1473 823 360 184 462 1604 3489 Fragments arranged by size: 3489 1604 1473 823 462 360 184 BsrBI CCG'CTC Cuts at: 1394 1823 2362 3183 4056 6282 1394 Size: 429 539 821 873 2226 3507 Fragments arranged by size: 3507 2226 873 821 539 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4105 5107 5157 5245 5631 6890 7064 Size: 1183 2857 1002 50 88 386 1259 174 Cuts at: 7064 65 Size: 1396 Fragments arranged by size: 2857 1396 1259 1183 1002 386 174 88 50 BsrFI r'CCGG_y Cuts at: 1700 1810 2114 2182 2350 4101 7039 7226 1700 Size: 110 304 68 168 1751 2938 187 2869 Fragments arranged by size: 2938 2869 1751 304 187 168 110 68 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8395 BssHII G'CGCG_C Cuts at: 1925 2033 1925 Size: 108 8287 BssSI C'ACGA_G Cuts at: 3881 6455 7467 3881 Size: 2574 1012 4809 Fragments arranged by size: 4809 2574 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3016 3646 794 Size: 2222 630 5543 Fragments arranged by size: 5543 2222 630 BstBI TT'CG_AA Cuts at: 2007 7199 2007 Size: 5192 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8395 BstXI CCAn_nnnn'nTGG Cuts at: 4332 4846 4332 Size: 514 7881 BstYI r'GATC_y Cuts at: 1972 3138 3831 4683 5151 5667 5725 6092 6477 Size: 1166 693 852 468 516 58 367 385 Cuts at: 6477 6494 7946 8032 8044 8390 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1166 852 693 516 468 385 367 346 86 58 17 12 Bsu36I CC'TnA_GG Cuts at: 3703 5586 3703 Size: 1883 6512 BtgI C'CryG_G Cuts at: 513 1065 2022 2204 2534 2619 2988 3105 3444 Size: 552 957 182 330 85 369 117 339 Cuts at: 3444 513 Size: 5464 Fragments arranged by size: 5464 957 552 369 339 330 182 117 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7656 Fragments arranged by size: 7656 683 56 BtsI GCAGTG_nn' Cuts at: 2217 2463 3640 5911 6705 6725 2217 Size: 246 1177 2271 794 20 3887 Fragments arranged by size: 3887 2271 1177 794 246 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8395 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2169 2204 529 Size: 35 1605 35 6720 Fragments arranged by size: 6720 1605 35 35 DraI TTT'AAA Cuts at: 5461 5699 5461 Size: 238 8157 DraIII CAC_nnn'GTG Cuts at: 5570 5570 Size: 8395 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4733 5618 7402 809 Size: 507 3417 885 1784 1802 Fragments arranged by size: 3417 1802 1784 885 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2177 2214 Size: 54 1425 50 275 18 282 65 37 Cuts at: 2214 2225 2472 3057 3441 3885 4039 5842 6731 Size: 11 247 585 384 444 154 1803 889 Cuts at: 6731 8360 8 Size: 1629 43 Fragments arranged by size: 1803 1629 1425 889 585 444 384 282 275 247 154 65 54 50 43 37 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2472 5842 1537 Size: 275 660 3370 4090 Fragments arranged by size: 4090 3370 660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3244 3271 3837 4745 6325 1353 Size: 1891 27 566 908 1580 3423 Fragments arranged by size: 3423 1891 1580 908 566 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3092 4420 6982 7356 7502 387 Size: 1064 138 1503 1328 2562 374 146 1280 Fragments arranged by size: 2562 1503 1328 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3772 4205 5117 3772 Size: 433 912 7050 Fragments arranged by size: 7050 912 433 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2577 4030 4081 4621 5169 5333 5359 Size: 393 945 1453 51 540 548 164 26 Cuts at: 5359 6458 7055 7842 8110 1239 Size: 1099 597 787 268 1524 Fragments arranged by size: 1524 1453 1099 945 787 597 548 540 393 268 164 51 26 EcoNI CCTnn'n_nnAGG Cuts at: 2504 4562 4985 2504 Size: 2058 423 5914 Fragments arranged by size: 5914 2058 423 EcoO109I rG'GnC_Cy Cuts at: 2107 4489 4851 5248 2107 Size: 2382 362 397 5254 Fragments arranged by size: 5254 2382 397 362 EcoRI G'AATT_C Cuts at: 5704 6082 5704 Size: 378 8017 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3189 3189 Size: 8395 FspI TGC'GCA Cuts at: 6901 6901 Size: 8395 HaeII r_GCGC'y Cuts at: 1678 2047 2069 2121 2272 2534 3598 7542 1678 Size: 369 22 52 151 262 1064 3944 2531 Fragments arranged by size: 3944 2531 1064 369 262 151 52 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2131 2163 2225 2257 2398 Size: 32 135 32 559 32 62 32 141 Cuts at: 2398 2430 4073 4105 4227 4259 5344 5376 7036 Size: 32 1643 32 122 32 1085 32 1660 Cuts at: 7036 7068 7110 7142 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1660 1643 1085 559 141 135 122 62 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2100 2721 3988 4816 4844 5739 Size: 988 281 162 621 1267 828 28 895 Cuts at: 5739 5985 669 Size: 246 3079 Fragments arranged by size: 3079 1267 988 895 828 621 281 246 162 28 HindIII A'AGCT_T Cuts at: 748 2393 748 Size: 1645 6750 HpaI GTT'AAC Cuts at: 5985 5985 Size: 8395 KasI G'GCGC_C Cuts at: 2065 2117 2268 2530 3594 2065 Size: 52 151 262 1064 6866 Fragments arranged by size: 6866 1064 262 151 52 KpnI G_GTAC'C Cuts at: 5720 5720 Size: 8395 MfeI C'AATT_G Cuts at: 4067 4267 5994 4067 Size: 200 1727 6468 Fragments arranged by size: 6468 1727 200 MluI A'CGCG_T Cuts at: 3419 8271 3419 Size: 4852 3543 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2825 4440 5524 7509 7693 1305 Size: 1520 1615 1084 1985 184 2007 Fragments arranged by size: 2007 1985 1615 1520 1084 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 3443 3887 4041 10 Size: 54 1425 343 384 1227 444 154 4364 Fragments arranged by size: 4364 1425 1227 444 384 343 154 54 MslI CAynn'nnrTG Cuts at: 518 1166 3095 4559 5162 5405 6353 6712 6871 Size: 648 1929 1464 603 243 948 359 159 Cuts at: 6871 518 Size: 2042 Fragments arranged by size: 2042 1929 1464 948 648 603 359 243 159 MspA1I CmG'CkG Cuts at: 1606 2206 2536 4733 5350 5811 6488 7636 7881 Size: 600 330 2197 617 461 677 1148 245 Cuts at: 7881 1606 Size: 2120 Fragments arranged by size: 2197 2120 1148 677 617 600 461 330 245 NaeI GCC'GGC Cuts at: 2116 4103 2116 Size: 1987 6408 NarI GG'CG_CC Cuts at: 2066 2118 2269 2531 3595 2066 Size: 52 151 262 1064 6866 Fragments arranged by size: 6866 1064 262 151 52 NcoI C'CATG_G Cuts at: 513 1065 2022 2619 2988 3444 513 Size: 552 957 597 369 456 5464 Fragments arranged by size: 5464 957 597 552 456 369 NdeI CA'TA_TG Cuts at: 387 387 Size: 8395 NgoMIV G'CCGG_C Cuts at: 2114 4101 2114 Size: 1987 6408 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8395 NotI GC'GGCC_GC Cuts at: 5842 5842 Size: 8395 NsiI A_TGCA'T Cuts at: 3021 3021 Size: 8395 NspI r_CATG'y Cuts at: 1132 1229 3331 4751 4772 5753 7298 1132 Size: 97 2102 1420 21 981 1545 2229 Fragments arranged by size: 2229 2102 1545 1420 981 97 21 PasI CC'CwG_GG Cuts at: 1348 4216 1348 Size: 2868 5527 PciI A'CATG_T Cuts at: 7294 7294 Size: 8395 PflMI CCAn_nnn'nTGG Cuts at: 1108 2223 2620 5296 1108 Size: 1115 397 2676 4207 Fragments arranged by size: 4207 2676 1115 397 PfoI T'CCnGG_A Cuts at: 2149 3141 3210 4986 2149 Size: 992 69 1776 5558 Fragments arranged by size: 5558 1776 992 69 PmeI GTTT'AAAC Cuts at: 5699 5699 Size: 8395 PmlI CAC'GTG Cuts at: 4666 4666 Size: 8395 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6464 6496 7977 8009 6464 Size: 32 1481 32 6850 Fragments arranged by size: 6850 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 4851 5248 2107 Size: 2744 397 5254 Fragments arranged by size: 5254 2744 397 PshAI GACnn'nnGTC Cuts at: 1613 2105 1613 Size: 492 7903 PsiI TTA'TAA Cuts at: 5965 5965 Size: 8395 PstI C_TGCA'G Cuts at: 830 3137 4367 5747 830 Size: 2307 1230 1380 3478 Fragments arranged by size: 3478 2307 1380 1230 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8395 PvuII CAG'CTG Cuts at: 4733 5350 4733 Size: 617 7778 SacI G_AGCT'C Cuts at: 3774 4207 5119 3774 Size: 433 912 7050 Fragments arranged by size: 7050 912 433 SacII CC_GC'GG Cuts at: 2207 2537 2207 Size: 330 8065 SalI G'TCGA_C Cuts at: 1936 2098 2719 5737 1936 Size: 162 621 3018 4594 Fragments arranged by size: 4594 3018 621 162 SanDI GG'GwC_CC Cuts at: 4851 4851 Size: 8395 SapI GCTCTTCn'nnn_ Cuts at: 3244 3244 Size: 8395 SbfI CC_TGCA'GG Cuts at: 3137 5747 3137 Size: 2610 5785 ScaI AGT'ACT Cuts at: 3311 6643 8355 3311 Size: 3332 1712 3351 Fragments arranged by size: 3351 3332 1712 SexAI A'CCwGG_T Cuts at: 3081 3552 3081 Size: 471 7924 SfcI C'TryA_G Cuts at: 826 1045 2959 2980 3133 3750 4363 5743 6878 Size: 219 1914 21 153 617 613 1380 1135 Cuts at: 6878 7559 7750 826 Size: 681 191 1471 Fragments arranged by size: 1914 1471 1380 1135 681 617 613 219 191 153 21 SfoI GGC'GCC Cuts at: 2067 2119 2270 2532 3596 2067 Size: 52 151 262 1064 6866 Fragments arranged by size: 6866 1064 262 151 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8395 SgrAI Cr'CCGG_yG Cuts at: 1700 2182 1700 Size: 482 7913 SmaI CCC'GGG Cuts at: 1872 2516 5722 1872 Size: 644 3206 4545 Fragments arranged by size: 4545 3206 644 SmlI C'TyrA_G Cuts at: 820 1017 1943 5493 6499 7400 7662 820 Size: 197 926 3550 1006 901 262 1553 Fragments arranged by size: 3550 1553 1006 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5692 6186 493 Size: 5199 494 2702 Fragments arranged by size: 5199 2702 494 SpeI A'CTAG_T Cuts at: 152 3242 5681 152 Size: 3090 2439 2866 Fragments arranged by size: 3090 2866 2439 SphI G_CATG'C Cuts at: 1132 5753 1132 Size: 4621 3774 SspI AAT'ATT Cuts at: 5 52 4182 5018 6319 5 Size: 47 4130 836 1301 2081 Fragments arranged by size: 4130 2081 1301 836 47 StuI AGG'CCT Cuts at: 1517 3284 1517 Size: 1767 6628 StyI C'CwwG_G Cuts at: 513 1065 2022 2619 2988 3444 4113 4369 4455 Size: 552 957 597 369 456 669 256 86 Cuts at: 4455 4717 5474 5834 513 Size: 262 757 360 3074 Fragments arranged by size: 3074 957 757 669 597 552 456 369 360 262 256 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 2938 3049 3913 4523 5224 6422 Size: 1255 873 485 111 864 610 701 1198 Cuts at: 6422 6439 6592 6777 7222 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1198 873 864 701 610 485 445 185 153 111 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3309 3341 3846 Size: 274 80 33 51 1443 1332 32 505 Cuts at: 3846 4446 6641 8353 96 Size: 600 2195 1712 138 Fragments arranged by size: 2195 1712 1443 1332 600 505 274 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3621 3653 3729 3761 3958 3990 4265 4297 4301 Size: 32 76 32 197 32 275 32 4 Cuts at: 4301 4333 8355 8387 3621 Size: 32 4022 32 3629 Fragments arranged by size: 4022 3629 275 197 76 32 32 32 32 32 32 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8395 XbaI T'CTAG_A Cuts at: 5731 5731 Size: 8395 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4469 4601 4967 4469 Size: 132 366 7897 Fragments arranged by size: 7897 366 132 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8395 XmaI C'CCGG_G Cuts at: 1870 2514 5720 1870 Size: 644 3206 4545 Fragments arranged by size: 4545 3206 644 XmnI GAAnn'nnTTC Cuts at: 4181 6524 4181 Size: 2343 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6203 276 Size: 53 83 186 936 4669 2468 Fragments arranged by size: 4669 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI PacI PspOMI PspXI PsrI RsrII SfiI SrfI SwaI