Restriction map for
pFN21ASDA1058
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# NO CUT ENZYMES ( 51 enzymes) #
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AatII Acc65I AclI AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AvrII BbvCI BlnI
BsiWI BspDI BssHII BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraIII Eam1105I EcoO65I FseI KpnI
KspI NheI NotI NruI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Aor51HI 5183 AGCGCT AGC^GCT
ApaI 114 GGGCCC GGGCC^C
AspI 260 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5158 TGCGCA TGC^GCA
BanI 3679 GGCGCC G^G[CT][GA]CC
BbeI 3679 GGCGCC GGCGC^C
BbrPI 2581 CACGTG CAC^GTG
BglII 659 AGATCT A^GATCT
BsaBI 3399 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5900 CGGCCG CG[GA][CT]^CG
Bsp1407I 539 TGTACA T^GTACA
BspLU11I 4415 ACATGT A^CATGT
BsrBI 4409 GAGCGG CCGCTC(-3/-3)
BsrGI 539 TGTACA T^GTACA
Bst1107I 2460 GTATAC GTA^TAC
BstZ17I 2460 GTATAC GTA^TAC
DraI 4341 TTTAAA TTT^AAA
EagI 5900 CGGCCG C^GGCCG
EclXI 5900 CGGCCG C^GGCCG
Eco47III 5183 AGCGCT AGC^GCT
Eco52I 5900 CGGCCG C^GGCCG
EcoRI 2002 GAATTC G^AATTC
EcoRV 6017 GATATC GAT^ATC
FspI 5158 TGCGCA TGC^GCA
HpaI 2662 GTTAAC GTT^AAC
KasI 3679 GGCGCC G^GCGCC
MamI 3399 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1760 CAATTG C^AATTG
MluI 2747 ACGCGT A^CGCGT
MunI 1760 CAATTG C^AATTG
NaeI 123 GCCGGC GCC^GGC
NarI 3679 GGCGCC GG^CGCC
NcoI 4263 CCATGG C^CATGG
NgoMIV 123 GCCGGC G^CCGGC
PaeR7I 6124 CTCGAG C^TCGAG
PflFI 260 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2581 CACGTG CAC^GTG
PmlI 2581 CACGTG CAC^GTG
SalI 472 GTCGAC G^TCGAC
SexAI 2058 ACCTGGT A^CC[AT]GGT
SfoI 3679 GGCGCC GGC^GCC
SmaI 111 CCCGGG CCC^GGG
SspBI 539 TGTACA T^GTACA
Tth111I 260 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1749 TCTAGA T^CTAGA
XcmI 4869 CCATGTCAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 6124 CTCGAG C^TCGAG
XmaCI 111 CCCGGG C^CCGGG
XmaI 111 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccIII 2391 TCCGGA T^CCGGA
4550 TCCGGA
AcyI 3627 GACGCC G[GA]^CG[CT]C
3679 GGCGCC
AflIII 2747 ACGCGT A^C[GA][CT]GT
4415 ACATGT
Asp700I 1257 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1382 GAATATTTTC
BcgI 268 CGATACATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5190 CGAGCTCATTGC
BciVI 4721 GGATAC GTATCC(6/5)
1368 GTATCC
BclI 2897 TGATCA T^GATCA
5915 TGATCA
BsaHI 3627 GACGCC G[GA]^CG[CT]C
3679 GGCGCC
BsaI 3164 GAGACC GGTCTC(1/5)
2325 GGTCTC
BseAI 2391 TCCGGA T^CCGGA
4550 TCCGGA
BsmBI 255 GAGACG CGTCTC(1/5)
591 GAGACG
BspEI 2391 TCCGGA T^CCGGA
4550 TCCGGA
BspHI 1350 TCATGA T^CATGA
4280 TCATGA
BsrFI 123 GCCGGC [GA]^CCGG[CT]
4667 ACCGGC
Cfr10I 123 GCCGGC [GA]^CCGG[CT]
4667 ACCGGC
DsaI 3860 CCGTGG C^C[GA][CT]GG
4263 CCATGG
EcoNI 3660 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5000 CCTGCCTGAGG
FauI 3571 GCGGG CCCGC(4/6)
5726 GCGGG
FbaI 2897 TGATCA T^GATCA
5915 TGATCA
HgaI 3627 GACGC GACGC(5/10)
4144 GACGC
Hin1I 3627 GACGCC G[GA]^CG[CT]C
3679 GGCGCC
HindIII 1036 AAGCTT A^AGCTT
6071 AAGCTT
MroI 2391 TCCGGA T^CCGGA
4550 TCCGGA
RcaI 1350 TCATGA T^CATGA
4280 TCATGA
StuI 4484 AGGCCT AGG^CCT
5081 AGGCCT
XmnI 1257 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1382 GAATATTTTC
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# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BamHI BanII BbsI BbvI BcnI
BfaI BfrI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DrdI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII