(Circular) (Six-base) MAPSORT of: pFN21ASDA1058.seq Check: 2015 from: 1 to: 10933 pFN21ASDA1058 10933 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8743 278 Size: 53 83 186 936 7207 2468 Fragments arranged by size: 7207 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2496 3273 4484 6324 8276 1937 Size: 559 777 1211 1840 1952 4594 Fragments arranged by size: 4594 1952 1840 1211 777 559 Acc65I G'GTAC_C Cuts at: 8254 8254 Size: 10933 AclI AA'CG_TT Cuts at: 1563 9060 9433 1563 Size: 7497 373 3063 Fragments arranged by size: 7497 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3452 3563 4910 6054 7057 8996 10380 10648 Size: 1820 111 1347 1144 1003 1939 1384 268 Cuts at: 10648 1632 Size: 1917 Fragments arranged by size: 1939 1917 1820 1384 1347 1144 1003 268 111 AfeI AGC'GCT Cuts at: 7208 7208 Size: 10933 AflII C'TTAA_G Cuts at: 820 1017 4061 4577 6385 8066 820 Size: 197 3044 516 1808 1681 3687 Fragments arranged by size: 3687 3044 1808 1681 516 197 AflIII A'CryG_T Cuts at: 1203 4770 6438 9832 10809 1203 Size: 3567 1668 3394 977 1327 Fragments arranged by size: 3567 3394 1668 1327 977 AgeI A'CCGG_T Cuts at: 9764 9764 Size: 10933 AhdI GACnn_n'nnGTC Cuts at: 9662 9662 Size: 10933 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10933 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4463 6943 1184 Size: 3279 2480 5174 Fragments arranged by size: 5174 3279 2480 AlwNI CAG_nnn'CTG Cuts at: 2215 2312 6115 6277 6658 8676 10248 2215 Size: 97 3803 162 381 2018 1572 2900 Fragments arranged by size: 3803 2900 2018 1572 381 162 97 ApaI G_GGCC'C Cuts at: 2141 2141 Size: 10933 ApaLI G'TGCA_C Cuts at: 8990 10146 8990 Size: 1156 9777 ApoI r'AATT_y Cuts at: 1493 2662 3890 4025 4145 4455 5219 5878 7500 Size: 1169 1228 135 120 310 764 659 1622 Cuts at: 7500 7535 8242 8468 8620 10729 1493 Size: 35 707 226 152 2109 1697 Fragments arranged by size: 2109 1697 1622 1228 1169 764 707 659 310 226 152 135 120 35 AseI AT'TA_AT Cuts at: 160 1032 9487 160 Size: 872 8455 1606 Fragments arranged by size: 8455 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10933 AvaI C'yCGr_G Cuts at: 1870 1943 2134 4695 6851 7339 8147 8258 1870 Size: 73 191 2561 2156 488 808 111 4545 Fragments arranged by size: 4545 2561 2156 808 488 191 111 73 BamHI G'GATC_C Cuts at: 2648 3120 6888 8263 8630 2648 Size: 472 3768 1375 367 4951 Fragments arranged by size: 4951 3768 1375 472 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5702 8254 9709 618 Size: 325 217 627 3915 2552 1455 1842 Fragments arranged by size: 3915 2552 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2135 2141 2772 3350 4546 6494 6858 7170 Size: 744 6 631 578 1196 1948 364 312 Cuts at: 7170 7218 7905 8252 1391 Size: 48 687 347 4072 Fragments arranged by size: 4072 1948 1196 744 687 631 578 364 347 312 48 6 BbeI G_GCGC'C Cuts at: 5706 5706 Size: 10933 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2210 4053 5095 5137 6367 6869 7125 Size: 971 311 1843 1042 42 1230 502 256 Cuts at: 7125 8145 928 Size: 1020 3716 Fragments arranged by size: 3716 1843 1230 1042 1020 971 502 311 256 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2280 2314 7202 7236 9124 9158 1903 Size: 34 343 34 4888 34 1888 34 3678 Fragments arranged by size: 4888 3678 1888 343 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3402 6738 8219 8825 10035 3402 Size: 3336 1481 606 1210 4300 Fragments arranged by size: 4300 3336 1481 1210 606 BclI T'GATC_A Cuts at: 1548 4920 7938 1548 Size: 3372 3018 4543 Fragments arranged by size: 4543 3372 3018 BfrBI ATG'CAT Cuts at: 3626 6097 8212 3626 Size: 2471 2115 6347 Fragments arranged by size: 6347 2471 2115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3557 6953 7044 Size: 107 122 71 631 605 1885 3396 91 Cuts at: 7044 9544 136 Size: 2500 1525 Fragments arranged by size: 3396 2500 1885 1525 631 605 122 107 91 71 BglII A'GATC_T Cuts at: 2682 10928 2682 Size: 8246 2687 BlpI GC'TnA_GC Cuts at: 3078 3492 3747 4782 4935 6488 8350 3078 Size: 414 255 1035 153 1553 1862 5661 Fragments arranged by size: 5661 1862 1553 1035 414 255 153 Bme1580I G_kGCm'C Cuts at: 1790 2141 3900 8994 10150 1790 Size: 351 1759 5094 1156 2573 Fragments arranged by size: 5094 2573 1759 1156 351 BmgBI CAC'GTC Cuts at: 1318 3315 6867 7123 7630 1318 Size: 1997 3552 256 507 4621 Fragments arranged by size: 4621 3552 1997 507 256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3749 4714 8664 Size: 416 53 83 399 24 2332 965 3950 Cuts at: 8664 9622 442 Size: 958 1753 Fragments arranged by size: 3950 2332 1753 965 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10933 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4066 5799 5801 7123 4066 Size: 1733 2 1322 7876 Fragments arranged by size: 7876 1733 1322 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2843 4560 5205 5297 5381 6800 7943 9593 Size: 1604 1717 645 92 84 1419 1143 1650 Cuts at: 9593 1239 Size: 2579 Fragments arranged by size: 2579 1717 1650 1604 1419 1143 645 92 84 Bpu10I CC'TnA_GC Cuts at: 2109 6857 8110 2109 Size: 4748 1253 4932 Fragments arranged by size: 4932 4748 1253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2756 3344 3692 4222 4771 4835 5423 6452 7193 Size: 588 348 530 549 64 588 1029 741 Cuts at: 7193 9058 9923 10221 2756 Size: 1865 865 298 3468 Fragments arranged by size: 3468 1865 1029 865 741 588 588 549 530 348 298 64 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4354 5181 9596 882 Size: 617 2855 827 4415 2219 Fragments arranged by size: 4415 2855 2219 827 617 BsaAI yAC'GTr Cuts at: 493 1204 3271 4606 6940 8230 8724 493 Size: 711 2067 1335 2334 1290 494 2702 Fragments arranged by size: 2702 2334 2067 1335 1290 711 494 BsaBI GATnn'nnATC Cuts at: 5426 10927 5426 Size: 5501 5432 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5651 5703 8740 9122 Size: 53 83 186 936 4118 52 3037 382 Cuts at: 9122 9757 275 Size: 635 1451 Fragments arranged by size: 4118 3037 1451 936 635 382 186 83 53 52 BsaWI w'CCGG_w Cuts at: 2948 3246 4414 6573 9366 9764 10038 10185 10596 Size: 298 1168 2159 2793 398 274 147 411 Cuts at: 10596 2948 Size: 3285 Fragments arranged by size: 3285 2793 2159 1168 411 398 298 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5725 5755 1178 Size: 30 165 30 4322 30 6356 Fragments arranged by size: 6356 4322 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2504 6706 7045 7048 7115 7909 1186 Size: 1318 4202 339 3 67 794 4210 Fragments arranged by size: 4210 4202 1318 794 339 67 3 BseYI C'CCAG_C Cuts at: 5698 6115 6513 8221 10136 10632 5698 Size: 417 398 1708 1915 496 5999 Fragments arranged by size: 5999 1915 1708 496 417 398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5669 6029 6164 7286 1163 Size: 573 3933 360 135 1122 4810 Fragments arranged by size: 4810 3933 1122 573 360 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7926 8383 9144 10172 1540 Size: 275 186 5925 457 761 1028 2301 Fragments arranged by size: 5925 2301 1028 761 457 275 186 BsiHKAI G_wGCw'C Cuts at: 2772 5439 6494 6858 7170 7218 7905 7919 8994 Size: 2667 1055 364 312 48 687 14 1075 Cuts at: 8994 9079 10150 2772 Size: 85 1071 3555 Fragments arranged by size: 3555 2667 1075 1071 1055 687 364 312 85 48 14 BsmI GAATG_Cn' Cuts at: 3553 3591 5458 8444 8537 3553 Size: 38 1867 2986 93 5949 Fragments arranged by size: 5949 2986 1867 93 38 BsmBI CGTCTCn'nnnn_ Cuts at: 2272 2608 2272 Size: 336 10597 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2141 2772 3350 3900 4546 5439 Size: 399 345 6 631 578 550 646 893 Cuts at: 5439 6494 6858 7170 7218 7905 7919 8252 8994 Size: 1055 364 312 48 687 14 333 742 Cuts at: 8994 9079 10150 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1055 893 742 687 646 631 578 550 399 364 345 333 312 85 48 14 6 BspEI T'CCGG_A Cuts at: 4414 6573 10596 4414 Size: 2159 4023 4751 Fragments arranged by size: 4751 4023 2159 BspHI T'CATG_A Cuts at: 3373 6303 8822 10552 3373 Size: 2930 2519 1730 3754 Fragments arranged by size: 3754 2930 2519 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2973 3863 5454 5526 5703 8288 844 Size: 2129 890 1591 72 177 2585 3489 Fragments arranged by size: 3489 2585 2129 1591 890 177 72 BsrBI CCG'CTC Cuts at: 1394 1823 6434 8820 1394 Size: 429 4611 2386 3507 Fragments arranged by size: 4611 3507 2386 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2886 3142 3164 3484 5715 6204 6930 Size: 1183 1638 256 22 320 2231 489 726 Cuts at: 6930 7218 9428 9602 65 Size: 288 2210 174 1396 Fragments arranged by size: 2231 2210 1638 1396 1183 726 489 320 288 256 174 22 BsrFI r'CCGG_y Cuts at: 1700 1810 2146 6690 9577 9764 1700 Size: 110 336 4544 2887 187 2869 Fragments arranged by size: 4544 2887 2869 336 187 110 BsrGI T'GTAC_A Cuts at: 96 2562 96 Size: 2466 8467 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10933 BssSI C'ACGA_G Cuts at: 8993 10005 8993 Size: 1012 9921 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 10933 BstBI TT'CG_AA Cuts at: 2007 9737 2007 Size: 7730 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10933 BstXI CCAn_nnnn'nTGG Cuts at: 2537 3597 4391 4468 6155 7867 2537 Size: 1060 794 77 1687 1712 5603 Fragments arranged by size: 5603 1712 1687 1060 794 77 BstYI r'GATC_y Cuts at: 1972 2211 2648 2682 2853 3120 5780 6888 7591 Size: 239 437 34 171 267 2660 1108 703 Cuts at: 7591 7685 8263 8630 9015 9032 10484 10570 10582 Size: 94 578 367 385 17 1452 86 12 Cuts at: 10582 10928 1972 Size: 346 1977 Fragments arranged by size: 2660 1977 1452 1108 703 578 437 385 367 346 267 239 171 94 86 34 17 12 BstZ17I GTA'TAC Cuts at: 4485 4485 Size: 10933 Bsu36I CC'TnA_GG Cuts at: 4552 7028 7587 4552 Size: 2476 559 7898 Fragments arranged by size: 7898 2476 559 BtgI C'CryG_G Cuts at: 513 1065 5883 6286 513 Size: 552 4818 403 5160 Fragments arranged by size: 5160 4818 552 403 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2658 4607 487 Size: 683 56 1432 1949 6813 Fragments arranged by size: 6813 1949 1432 683 56 BtsI GCAGTG_nn' Cuts at: 3343 4445 5013 6378 7184 7769 7795 8449 9243 Size: 1102 568 1365 806 585 26 654 794 Cuts at: 9243 9263 3343 Size: 20 5013 Fragments arranged by size: 5013 1365 1102 806 794 654 585 568 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10933 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10898 DraI TTT'AAA Cuts at: 6366 8237 6366 Size: 1871 9062 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2286 2492 2602 9940 809 Size: 507 970 206 110 7338 1802 Fragments arranged by size: 7338 1802 970 507 206 110 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2268 3447 6157 Size: 54 1425 50 275 18 438 1179 2710 Cuts at: 6157 6279 6762 6828 6882 7869 7923 8380 9269 Size: 122 483 66 54 987 54 457 889 Cuts at: 9269 10898 8 Size: 1629 43 Fragments arranged by size: 2710 1629 1425 1179 987 889 483 457 438 275 122 66 54 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7923 8380 1537 Size: 275 6111 457 4090 Fragments arranged by size: 6111 4090 457 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2319 3096 3523 4253 5828 7733 7752 Size: 896 70 777 427 730 1575 1905 19 Cuts at: 7752 8863 1353 Size: 1111 3423 Fragments arranged by size: 3423 1905 1575 1111 896 777 730 427 70 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2336 7688 7913 9520 9894 10040 Size: 1064 138 747 5352 225 1607 374 146 Cuts at: 10040 387 Size: 1280 Fragments arranged by size: 5352 1607 1280 1064 747 374 225 146 138 EcoICRI GAG'CTC Cuts at: 2770 6492 6856 7168 7216 7903 2770 Size: 3722 364 312 48 687 5800 Fragments arranged by size: 5800 3722 687 364 312 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2843 3452 3563 4560 4910 5205 5297 Size: 393 1211 609 111 997 350 295 92 Cuts at: 5297 5381 6054 6800 7057 7943 8996 9593 10380 Size: 84 673 746 257 886 1053 597 787 Cuts at: 10380 10648 1239 Size: 268 1524 Fragments arranged by size: 1524 1211 1053 997 886 787 746 673 609 597 393 350 295 268 257 111 92 84 EcoNI CCTnn'n_nnAGG Cuts at: 5687 7027 5687 Size: 1340 9593 EcoO109I rG'GnC_Cy Cuts at: 2138 3185 5692 6390 7359 2138 Size: 1047 2507 698 969 5712 Fragments arranged by size: 5712 2507 1047 969 698 EcoRI G'AATT_C Cuts at: 4025 8242 8620 4025 Size: 4217 378 6338 Fragments arranged by size: 6338 4217 378 EcoRV GAT'ATC Cuts at: 8042 8042 Size: 10933 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3082 5218 6583 3082 Size: 2136 1365 7432 Fragments arranged by size: 7432 2136 1365 FspI TGC'GCA Cuts at: 7183 9439 7183 Size: 2256 8677 HaeII r_GCGC'y Cuts at: 1678 3326 4110 5706 7023 7210 10080 1678 Size: 1648 784 1596 1317 187 2870 2531 Fragments arranged by size: 2870 2531 1648 1596 1317 784 187 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 3056 3088 9574 Size: 32 135 32 929 32 523 32 6486 Cuts at: 9574 9606 9648 9680 1373 Size: 32 42 32 2626 Fragments arranged by size: 6486 2626 929 523 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2485 2497 4687 7582 8277 8523 Size: 988 281 547 12 2190 2895 695 246 Cuts at: 8523 669 Size: 3079 Fragments arranged by size: 3079 2895 2190 988 695 547 281 246 12 HindIII A'AGCT_T Cuts at: 748 3059 8094 748 Size: 2311 5035 3587 Fragments arranged by size: 5035 3587 2311 HpaI GTT'AAC Cuts at: 4687 8523 4687 Size: 3836 7097 KasI G'GCGC_C Cuts at: 5702 5702 Size: 10933 KpnI G_GTAC'C Cuts at: 8258 8258 Size: 10933 MfeI C'AATT_G Cuts at: 3783 8532 3783 Size: 4749 6184 MluI A'CGCG_T Cuts at: 4770 10809 4770 Size: 6039 4894 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 7476 10047 10231 1305 Size: 1128 5043 2571 184 2007 Fragments arranged by size: 5043 2571 2007 1128 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3449 6159 6764 6830 6884 Size: 54 1425 343 1617 2710 605 66 54 Cuts at: 6884 7871 10 Size: 987 3072 Fragments arranged by size: 3072 2710 1617 1425 987 605 343 66 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2475 2591 2913 5126 5432 5603 6153 Size: 648 1309 116 322 2213 306 171 550 Cuts at: 6153 7290 7619 7865 8891 9250 9409 518 Size: 1137 329 246 1026 359 159 2042 Fragments arranged by size: 2213 2042 1309 1137 1026 648 550 359 329 322 306 246 171 159 116 MspA1I CmG'CkG Cuts at: 1606 3077 4183 4227 4642 5554 5633 5644 6115 Size: 1471 1106 44 415 912 79 11 471 Cuts at: 6115 6517 6610 7492 7912 8221 8349 9026 10174 Size: 402 93 882 420 309 128 677 1148 Cuts at: 10174 10419 1606 Size: 245 2120 Fragments arranged by size: 2120 1471 1148 1106 912 882 677 471 420 415 402 309 245 128 93 79 44 11 NaeI GCC'GGC Cuts at: 2148 2148 Size: 10933 NarI GG'CG_CC Cuts at: 5703 5703 Size: 10933 NcoI C'CATG_G Cuts at: 513 1065 6286 513 Size: 552 5221 5160 Fragments arranged by size: 5221 5160 552 NdeI CA'TA_TG Cuts at: 387 2297 4759 4789 8002 387 Size: 1910 2462 30 3213 3318 Fragments arranged by size: 3318 3213 2462 1910 30 NgoMIV G'CCGG_C Cuts at: 2146 2146 Size: 10933 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10933 NotI GC'GGCC_GC Cuts at: 8380 8380 Size: 10933 NsiI A_TGCA'T Cuts at: 3628 6099 8214 3628 Size: 2471 2115 6347 Fragments arranged by size: 6347 2471 2115 NspI r_CATG'y Cuts at: 1132 1229 3564 3706 4592 5962 6101 6442 8291 Size: 97 2335 142 886 1370 139 341 1849 Cuts at: 8291 9836 1132 Size: 1545 2229 Fragments arranged by size: 2335 2229 1849 1545 1370 886 341 142 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10933 PciI A'CATG_T Cuts at: 6438 9832 6438 Size: 3394 7539 PflMI CCAn_nnn'nTGG Cuts at: 1108 5359 6061 6277 1108 Size: 4251 702 216 5764 Fragments arranged by size: 5764 4251 702 216 PfoI T'CCnGG_A Cuts at: 5182 5397 5406 5182 Size: 215 9 10709 Fragments arranged by size: 10709 215 9 PmeI GTTT'AAAC Cuts at: 8237 8237 Size: 10933 PmlI CAC'GTG Cuts at: 4606 4606 Size: 10933 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3272 3304 7467 7499 9002 9034 10515 10547 3272 Size: 32 4163 32 1503 32 1481 32 3658 Fragments arranged by size: 4163 3658 1503 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3185 5692 6390 7359 3185 Size: 2507 698 969 6759 Fragments arranged by size: 6759 2507 969 698 PshAI GACnn'nnGTC Cuts at: 1613 4697 7328 7628 1613 Size: 3084 2631 300 4918 Fragments arranged by size: 4918 3084 2631 300 PsiI TTA'TAA Cuts at: 2670 6402 8503 2670 Size: 3732 2101 5100 Fragments arranged by size: 5100 3732 2101 PspOMI G'GGCC_C Cuts at: 2137 2137 Size: 10933 PspXI vC'TCGA_Gb Cuts at: 8147 8147 Size: 10933 PstI C_TGCA'G Cuts at: 830 3860 4120 5348 7950 8285 830 Size: 3030 260 1228 2602 335 3478 Fragments arranged by size: 3478 3030 2602 1228 335 260 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10933 PvuII CAG'CTG Cuts at: 3077 4183 4227 4642 5633 6115 6517 6610 7912 Size: 1106 44 415 991 482 402 93 1302 Cuts at: 7912 8221 3077 Size: 309 5789 Fragments arranged by size: 5789 1302 1106 991 482 415 402 309 93 44 SacI G_AGCT'C Cuts at: 2772 6494 6858 7170 7218 7905 2772 Size: 3722 364 312 48 687 5800 Fragments arranged by size: 5800 3722 687 364 312 48 SalI G'TCGA_C Cuts at: 1936 2495 8275 1936 Size: 559 5780 4594 Fragments arranged by size: 5780 4594 559 SapI GCTCTTCn'nnn_ Cuts at: 2249 5828 7752 2249 Size: 3579 1924 5430 Fragments arranged by size: 5430 3579 1924 SbfI CC_TGCA'GG Cuts at: 8285 8285 Size: 10933 ScaI AGT'ACT Cuts at: 3965 4520 4895 9181 10893 3965 Size: 555 375 4286 1712 4005 Fragments arranged by size: 4286 4005 1712 555 375 SexAI A'CCwGG_T Cuts at: 4081 4081 Size: 10933 SfcI C'TryA_G Cuts at: 826 1045 3667 3856 4116 4476 5344 7312 7946 Size: 219 2622 189 260 360 868 1968 634 Cuts at: 7946 8176 8281 9416 10097 10288 826 Size: 230 105 1135 681 191 1471 Fragments arranged by size: 2622 1968 1471 1135 868 681 634 360 260 230 219 191 189 105 SfoI GGC'GCC Cuts at: 5704 5704 Size: 10933 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10933 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10933 SmaI CCC'GGG Cuts at: 1872 2136 8260 1872 Size: 264 6124 4545 Fragments arranged by size: 6124 4545 264 SmlI C'TyrA_G Cuts at: 820 1017 1943 2771 3359 3707 4061 4237 4577 Size: 197 926 828 588 348 354 176 340 Cuts at: 4577 4750 4850 5438 6385 6467 7172 8066 8147 Size: 173 100 588 947 82 705 894 81 Cuts at: 8147 9037 9938 10200 820 Size: 890 901 262 1553 Fragments arranged by size: 1553 947 926 901 894 890 828 705 588 588 354 348 340 262 197 176 173 100 82 81 SnaBI TAC'GTA Cuts at: 493 8230 8724 493 Size: 7737 494 2702 Fragments arranged by size: 7737 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 10933 SphI G_CATG'C Cuts at: 1132 3564 4592 6101 8291 1132 Size: 2432 1028 1509 2190 3774 Fragments arranged by size: 3774 2432 2190 1509 1028 SrfI GCCC'GGGC Cuts at: 2136 2136 Size: 10933 SspI AAT'ATT Cuts at: 5 52 2709 3408 4009 8857 5 Size: 47 2657 699 601 4848 2081 Fragments arranged by size: 4848 2657 2081 699 601 47 StuI AGG'CCT Cuts at: 1517 6509 7106 1517 Size: 4992 597 5344 Fragments arranged by size: 5344 4992 597 StyI C'CwwG_G Cuts at: 513 1065 2521 3996 6286 8372 513 Size: 552 1456 1475 2290 2086 3074 Fragments arranged by size: 3074 2290 2086 1475 1456 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 7340 8960 8977 9130 9315 9760 Size: 1255 1137 4623 1620 17 153 185 445 Cuts at: 9760 325 Size: 1498 Fragments arranged by size: 4623 1620 1498 1255 1137 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2562 3963 4518 Size: 274 80 33 51 1443 585 1401 555 Cuts at: 4518 4893 4915 6057 6819 7524 9179 10891 96 Size: 375 22 1142 762 705 1655 1712 138 Fragments arranged by size: 1712 1655 1443 1401 1142 762 705 585 555 375 274 138 80 51 33 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4308 4340 5868 5900 7748 7780 10893 10925 4308 Size: 32 1528 32 1848 32 3113 32 4316 Fragments arranged by size: 4316 3113 1848 1528 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2286 1316 Size: 970 9963 XbaI T'CTAG_A Cuts at: 3772 8269 3772 Size: 4497 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6899 6899 Size: 10933 XhoI C'TCGA_G Cuts at: 1943 8147 1943 Size: 6204 4729 XmaI C'CCGG_G Cuts at: 1870 2134 8258 1870 Size: 264 6124 4545 Fragments arranged by size: 6124 4545 264 XmnI GAAnn'nnTTC Cuts at: 3284 3409 9062 3284 Size: 125 5653 5155 Fragments arranged by size: 5653 5155 125 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8741 276 Size: 53 83 186 936 7207 2468 Fragments arranged by size: 7207 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BsiWI DraIII FseI FspAI NruI PacI PsrI RsrII SacII SanDI SfiI SwaI