Restriction map for
pFN21ASDA1058
(Linear) (Six-base) MAPSORT of: KIAA1058.seq Check: 6588 from: 1 to: 6204
KIAA1058.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 473 1250 2461 4301 6204
Size: 473 777 1211 1840 1903
Fragments arranged by size:
1903 1840 1211 777 473
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1540 2887 4031 5034 6204
Size: 1429 111 1347 1144 1003 1170
Fragments arranged by size:
1429 1347 1170 1144 1003 111
AfeI AGC'GCT
Cuts at: 0 5185 6204
Size: 5185 1019
AflII C'TTAA_G
Cuts at: 0 2038 2554 4362 6043 6204
Size: 2038 516 1808 1681 161
Fragments arranged by size:
2038 1808 1681 516 161
AflIII A'CryG_T
Cuts at: 0 2747 4415 6204
Size: 2747 1668 1789
Fragments arranged by size:
2747 1789 1668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2440 4920 6204
Size: 2440 2480 1284
Fragments arranged by size:
2480 2440 1284
AlwNI CAG_nnn'CTG
Cuts at: 0 192 289 4092 4254 4635 6204
Size: 192 97 3803 162 381 1569
Fragments arranged by size:
3803 1569 381 192 162 97
ApaI G_GGCC'C
Cuts at: 0 118 6204
Size: 118 6086
ApoI r'AATT_y
Cuts at: 0 639 1867 2002 2122 2432 3196 3855 5477
Size: 639 1228 135 120 310 764 659 1622
Cuts at: 5477 5512 6204
Size: 35 692
Fragments arranged by size:
1622 1228 764 692 659 639 310 135
120 35
AvaI C'yCGr_G
Cuts at: 0 111 2672 4828 5316 6124 6204
Size: 111 2561 2156 488 808 80
Fragments arranged by size:
2561 2156 808 488 111 80
BamHI G'GATC_C
Cuts at: 0 625 1097 4865 6204
Size: 625 472 3768 1339
Fragments arranged by size:
3768 1339 625 472
BanI G'GyrC_C
Cuts at: 0 3679 6204
Size: 3679 2525
BanII G_rGCy'C
Cuts at: 0 112 118 749 1327 2523 4471 4835 5147
Size: 112 6 631 578 1196 1948 364 312
Cuts at: 5147 5195 5882 6204
Size: 48 687 322
Fragments arranged by size:
1948 1196 687 631 578 364 322 312
112 48 6
BbeI G_GCGC'C
Cuts at: 0 3683 6204
Size: 3683 2521
BbsI GAAGACnn'nnnn_
Cuts at: 0 187 2030 3072 3114 4344 4846 5102 6122
Size: 187 1843 1042 42 1230 502 256 1020
Cuts at: 6122 6204
Size: 82
Fragments arranged by size:
1843 1230 1042 1020 502 256 187 82
42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 257 291 5179 5213 6204
Size: 257 34 4888 34 991
Fragments arranged by size:
4888 991 257 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1379 4715 6204
Size: 1379 3336 1489
Fragments arranged by size:
3336 1489 1379
BclI T'GATC_A
Cuts at: 0 2897 5915 6204
Size: 2897 3018 289
Fragments arranged by size:
3018 2897 289
BfrBI ATG'CAT
Cuts at: 0 1603 4074 6189 6204
Size: 1603 2471 2115 15
Fragments arranged by size:
2471 2115 1603 15
BglI GCCn_nnn'nGGC
Cuts at: 0 1534 4930 5021 6204
Size: 1534 3396 91 1183
Fragments arranged by size:
3396 1534 1183 91
BglII A'GATC_T
Cuts at: 0 659 6204
Size: 659 5545
BlpI GC'TnA_GC
Cuts at: 0 1055 1469 1724 2759 2912 4465 6204
Size: 1055 414 255 1035 153 1553 1739
Fragments arranged by size:
1739 1553 1055 1035 414 255 153
Bme1580I G_kGCm'C
Cuts at: 0 118 1877 6204
Size: 118 1759 4327
Fragments arranged by size:
4327 1759 118
BmgBI CAC'GTC
Cuts at: 0 1292 4844 5100 5607 6204
Size: 1292 3552 256 507 597
Fragments arranged by size:
3552 1292 597 507 256
BmrI ACTGGGnnnn_n'
Cuts at: 0 1726 2691 6204
Size: 1726 965 3513
Fragments arranged by size:
3513 1726 965
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2043 3776 3778 5100 6204
Size: 2043 1733 2 1322 1104
Fragments arranged by size:
2043 1733 1322 1104 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 2537 3182 3274 3358 4777 5920 6204
Size: 820 1717 645 92 84 1419 1143 284
Fragments arranged by size:
1717 1419 1143 820 645 284 92 84
Bpu10I CC'TnA_GC
Cuts at: 0 86 4834 6087 6204
Size: 86 4748 1253 117
Fragments arranged by size:
4748 1253 117 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 1321 1669 2199 2748 2812 3400 4429
Size: 733 588 348 530 549 64 588 1029
Cuts at: 4429 5170 6204
Size: 741 1034
Fragments arranged by size:
1034 1029 741 733 588 588 549 530
348 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 2331 3158 6204
Size: 2331 827 3046
Fragments arranged by size:
3046 2331 827
BsaAI yAC'GTr
Cuts at: 0 1248 2583 4917 6204
Size: 1248 1335 2334 1287
Fragments arranged by size:
2334 1335 1287 1248
BsaBI GATnn'nnATC
Cuts at: 0 3403 6204
Size: 3403 2801
BsaHI Gr'CG_yC
Cuts at: 0 3628 3680 6204
Size: 3628 52 2524
Fragments arranged by size:
3628 2524 52
BsaWI w'CCGG_w
Cuts at: 0 925 1223 2391 4550 6204
Size: 925 298 1168 2159 1654
Fragments arranged by size:
2159 1654 1168 925 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3702 3732 6204
Size: 3702 30 2472
Fragments arranged by size:
3702 2472 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 481 4683 5022 5025 5092 5886 6204
Size: 481 4202 339 3 67 794 318
Fragments arranged by size:
4202 794 481 339 318 67 3
BseYI C'CCAG_C
Cuts at: 0 3675 4092 4490 6198 6204
Size: 3675 417 398 1708 6
Fragments arranged by size:
3675 1708 417 398 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3646 4006 4141 5263 6204
Size: 3646 360 135 1122 941
Fragments arranged by size:
3646 1122 941 360 135
BsiEI CG_ry'CG
Cuts at: 0 5903 6204
Size: 5903 301
BsiHKAI G_wGCw'C
Cuts at: 0 749 3416 4471 4835 5147 5195 5882 5896
Size: 749 2667 1055 364 312 48 687 14
Cuts at: 5896 6204
Size: 308
Fragments arranged by size:
2667 1055 749 687 364 312 308 48
14
BsmI GAATG_Cn'
Cuts at: 0 1530 1568 3435 6204
Size: 1530 38 1867 2769
Fragments arranged by size:
2769 1867 1530 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 249 585 6204
Size: 249 336 5619
Fragments arranged by size:
5619 336 249
Bsp1286I G_dGCh'C
Cuts at: 0 112 118 749 1327 1877 2523 3416 4471
Size: 112 6 631 578 550 646 893 1055
Cuts at: 4471 4835 5147 5195 5882 5896 6204
Size: 364 312 48 687 14 308
Fragments arranged by size:
1055 893 687 646 631 578 550 364
312 308 112 48 14 6
BspEI T'CCGG_A
Cuts at: 0 2391 4550 6204
Size: 2391 2159 1654
Fragments arranged by size:
2391 2159 1654
BspHI T'CATG_A
Cuts at: 0 1350 4280 6204
Size: 1350 2930 1924
Fragments arranged by size:
2930 1924 1350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 950 1840 3431 3503 3680 6204
Size: 950 890 1591 72 177 2524
Fragments arranged by size:
2524 1591 950 890 177 72
BsrBI CCG'CTC
Cuts at: 0 4411 6204
Size: 4411 1793
BsrDI GCAATG_nn'
Cuts at: 0 863 1119 1141 1461 3692 4181 4907 5195
Size: 863 256 22 320 2231 489 726 288
Cuts at: 5195 6204
Size: 1009
Fragments arranged by size:
2231 1009 863 726 489 320 288 256
22
BsrFI r'CCGG_y
Cuts at: 0 123 4667 6204
Size: 123 4544 1537
Fragments arranged by size:
4544 1537 123
BsrGI T'GTAC_A
Cuts at: 0 539 6204
Size: 539 5665
BstXI CCAn_nnnn'nTGG
Cuts at: 0 514 1574 2368 2445 4132 5844 6204
Size: 514 1060 794 77 1687 1712 360
Fragments arranged by size:
1712 1687 1060 794 514 360 77
BstYI r'GATC_y
Cuts at: 0 188 625 659 830 1097 3757 4865 5568
Size: 188 437 34 171 267 2660 1108 703
Cuts at: 5568 5662 6204
Size: 94 542
Fragments arranged by size:
2660 1108 703 542 437 267 188 171
94 34
BstZ17I GTA'TAC
Cuts at: 0 2462 6204
Size: 2462 3742
Bsu36I CC'TnA_GG
Cuts at: 0 2529 5005 5564 6204
Size: 2529 2476 559 640
Fragments arranged by size:
2529 2476 640 559
BtgI C'CryG_G
Cuts at: 0 3860 4263 6204
Size: 3860 403 1941
Fragments arranged by size:
3860 1941 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 635 2584 6204
Size: 635 1949 3620
Fragments arranged by size:
3620 1949 635
BtsI GCAGTG_nn'
Cuts at: 0 1320 2422 2990 4355 5161 5746 5772 6204
Size: 1320 1102 568 1365 806 585 26 432
Fragments arranged by size:
1365 1320 1102 806 585 568 432 26
DraI TTT'AAA
Cuts at: 0 4343 6204
Size: 4343 1861
DrdI GACnn_nn'nnGTC
Cuts at: 0 263 469 579 6204
Size: 263 206 110 5625
Fragments arranged by size:
5625 263 206 110
EaeI y'GGCC_r
Cuts at: 0 245 1424 4134 4256 4739 4805 4859 5846
Size: 245 1179 2710 122 483 66 54 987
Cuts at: 5846 5900 6204
Size: 54 304
Fragments arranged by size:
2710 1179 987 483 304 245 122 66
54 54
EagI C'GGCC_G
Cuts at: 0 5900 6204
Size: 5900 304
EarI CTCTTCn'nnn_
Cuts at: 0 226 296 1073 1500 2230 3805 5710 5729
Size: 226 70 777 427 730 1575 1905 19
Cuts at: 5729 6204
Size: 475
Fragments arranged by size:
1905 1575 777 730 475 427 226 70
19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 313 5665 5890 6204
Size: 313 5352 225 314
Fragments arranged by size:
5352 314 313 225
EcoICRI GAG'CTC
Cuts at: 0 747 4469 4833 5145 5193 5880 6204
Size: 747 3722 364 312 48 687 324
Fragments arranged by size:
3722 747 687 364 324 312 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1429 1540 2537 2887 3182 3274 3358
Size: 820 609 111 997 350 295 92 84
Cuts at: 3358 4031 4777 5034 5920 6204
Size: 673 746 257 886 284
Fragments arranged by size:
997 886 820 746 673 609 350 295
284 257 111 92 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3664 5004 6204
Size: 3664 1340 1200
Fragments arranged by size:
3664 1340 1200
EcoO109I rG'GnC_Cy
Cuts at: 0 115 1162 3669 4367 5336 6204
Size: 115 1047 2507 698 969 868
Fragments arranged by size:
2507 1047 969 868 698 115
EcoRI G'AATT_C
Cuts at: 0 2002 6204
Size: 2002 4202
EcoRV GAT'ATC
Cuts at: 0 6019 6204
Size: 6019 185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1059 3195 4560 6204
Size: 1059 2136 1365 1644
Fragments arranged by size:
2136 1644 1365 1059
FspI TGC'GCA
Cuts at: 0 5160 6204
Size: 5160 1044
HaeII r_GCGC'y
Cuts at: 0 1303 2087 3683 5000 5187 6204
Size: 1303 784 1596 1317 187 1017
Fragments arranged by size:
1596 1317 1303 1017 784 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 1033 1065 6204
Size: 478 32 523 32 5139
Fragments arranged by size:
5139 523 478 32 32
HincII GTy'rAC
Cuts at: 0 462 474 2664 5559 6204
Size: 462 12 2190 2895 645
Fragments arranged by size:
2895 2190 645 462 12
HindIII A'AGCT_T
Cuts at: 0 1036 6071 6204
Size: 1036 5035 133
Fragments arranged by size:
5035 1036 133
HpaI GTT'AAC
Cuts at: 0 2664 6204
Size: 2664 3540
KasI G'GCGC_C
Cuts at: 0 3679 6204
Size: 3679 2525
MfeI C'AATT_G
Cuts at: 0 1760 6204
Size: 1760 4444
MluI A'CGCG_T
Cuts at: 0 2747 6204
Size: 2747 3457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 5453 6204
Size: 410 5043 751
Fragments arranged by size:
5043 751 410
MscI TGG'CCA
Cuts at: 0 1426 4136 4741 4807 4861 5848 6204
Size: 1426 2710 605 66 54 987 356
Fragments arranged by size:
2710 1426 987 605 356 66 54
MslI CAynn'nnrTG
Cuts at: 0 452 568 890 3103 3409 3580 4130 5267
Size: 452 116 322 2213 306 171 550 1137
Cuts at: 5267 5596 5842 6204
Size: 329 246 362
Fragments arranged by size:
2213 1137 550 452 362 329 322 306
246 171 116
MspA1I CmG'CkG
Cuts at: 0 1054 2160 2204 2619 3531 3610 3621 4092
Size: 1054 1106 44 415 912 79 11 471
Cuts at: 4092 4494 4587 5469 5889 6198 6204
Size: 402 93 882 420 309 6
Fragments arranged by size:
1106 1054 912 882 471 420 415 402
309 93 79 44 11 6
NaeI GCC'GGC
Cuts at: 0 125 6204
Size: 125 6079
NarI GG'CG_CC
Cuts at: 0 3680 6204
Size: 3680 2524
NcoI C'CATG_G
Cuts at: 0 4263 6204
Size: 4263 1941
NdeI CA'TA_TG
Cuts at: 0 274 2736 2766 5979 6204
Size: 274 2462 30 3213 225
Fragments arranged by size:
3213 2462 274 225 30
NgoMIV G'CCGG_C
Cuts at: 0 123 6204
Size: 123 6081
NsiI A_TGCA'T
Cuts at: 0 1605 4076 6191 6204
Size: 1605 2471 2115 13
Fragments arranged by size:
2471 2115 1605 13
NspI r_CATG'y
Cuts at: 0 1541 1683 2569 3939 4078 4419 6204
Size: 1541 142 886 1370 139 341 1785
Fragments arranged by size:
1785 1541 1370 886 341 142 139
PciI A'CATG_T
Cuts at: 0 4415 6204
Size: 4415 1789
PflMI CCAn_nnn'nTGG
Cuts at: 0 3336 4038 4254 6204
Size: 3336 702 216 1950
Fragments arranged by size:
3336 1950 702 216
PfoI T'CCnGG_A
Cuts at: 0 3159 3374 3383 6204
Size: 3159 215 9 2821
Fragments arranged by size:
3159 2821 215 9
PmlI CAC'GTG
Cuts at: 0 2583 6204
Size: 2583 3621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1249 1281 5444 5476 6204
Size: 1249 32 4163 32 728
Fragments arranged by size:
4163 1249 728 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1162 3669 4367 5336 6204
Size: 1162 2507 698 969 868
Fragments arranged by size:
2507 1162 969 868 698
PshAI GACnn'nnGTC
Cuts at: 0 2674 5305 5605 6204
Size: 2674 2631 300 599
Fragments arranged by size:
2674 2631 599 300
PsiI TTA'TAA
Cuts at: 0 647 4379 6204
Size: 647 3732 1825
Fragments arranged by size:
3732 1825 647
PspOMI G'GGCC_C
Cuts at: 0 114 6204
Size: 114 6090
PspXI vC'TCGA_Gb
Cuts at: 0 6124 6204
Size: 6124 80
PstI C_TGCA'G
Cuts at: 0 1837 2097 3325 5927 6204
Size: 1837 260 1228 2602 277
Fragments arranged by size:
2602 1837 1228 277 260
PvuII CAG'CTG
Cuts at: 0 1054 2160 2204 2619 3610 4092 4494 4587
Size: 1054 1106 44 415 991 482 402 93
Cuts at: 4587 5889 6198 6204
Size: 1302 309 6
Fragments arranged by size:
1302 1106 1054 991 482 415 402 309
93 44 6
SacI G_AGCT'C
Cuts at: 0 749 4471 4835 5147 5195 5882 6204
Size: 749 3722 364 312 48 687 322
Fragments arranged by size:
3722 749 687 364 322 312 48
SalI G'TCGA_C
Cuts at: 0 472 6204
Size: 472 5732
SapI GCTCTTCn'nnn_
Cuts at: 0 226 3805 5729 6204
Size: 226 3579 1924 475
Fragments arranged by size:
3579 1924 475 226
ScaI AGT'ACT
Cuts at: 0 1942 2497 2872 6204
Size: 1942 555 375 3332
Fragments arranged by size:
3332 1942 555 375
SexAI A'CCwGG_T
Cuts at: 0 2058 6204
Size: 2058 4146
SfcI C'TryA_G
Cuts at: 0 1644 1833 2093 2453 3321 5289 5923 6153
Size: 1644 189 260 360 868 1968 634 230
Cuts at: 6153 6204
Size: 51
Fragments arranged by size:
1968 1644 868 634 360 260 230 189
51
SfoI GGC'GCC
Cuts at: 0 3681 6204
Size: 3681 2523
SmaI CCC'GGG
Cuts at: 0 113 6204
Size: 113 6091
SmlI C'TyrA_G
Cuts at: 0 748 1336 1684 2038 2214 2554 2727 2827
Size: 748 588 348 354 176 340 173 100
Cuts at: 2827 3415 4362 4444 5149 6043 6124 6204
Size: 588 947 82 705 894 81 80
Fragments arranged by size:
947 894 748 705 588 588 354 348
340 176 173 100 82 81 80
SphI G_CATG'C
Cuts at: 0 1541 2569 4078 6204
Size: 1541 1028 1509 2126
Fragments arranged by size:
2126 1541 1509 1028
SrfI GCCC'GGGC
Cuts at: 0 113 6204
Size: 113 6091
SspI AAT'ATT
Cuts at: 0 686 1385 1986 6204
Size: 686 699 601 4218
Fragments arranged by size:
4218 699 686 601
StuI AGG'CCT
Cuts at: 0 4486 5083 6204
Size: 4486 597 1121
Fragments arranged by size:
4486 1121 597
StyI C'CwwG_G
Cuts at: 0 498 1973 4263 6204
Size: 498 1475 2290 1941
Fragments arranged by size:
2290 1941 1475 498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 694 5317 6204
Size: 694 4623 887
Fragments arranged by size:
4623 887 694
TatI w'GTAC_w
Cuts at: 0 539 1940 2495 2870 2892 4034 4796 5501
Size: 539 1401 555 375 22 1142 762 705
Cuts at: 5501 6204
Size: 703
Fragments arranged by size:
1401 1142 762 705 703 555 539 375
22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2285 2317 3845 3877 5725 5757 6204
Size: 2285 32 1528 32 1848 32 447
Fragments arranged by size:
2285 1848 1528 447 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 263 6204
Size: 263 5941
XbaI T'CTAG_A
Cuts at: 0 1749 6204
Size: 1749 4455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4876 6204
Size: 4876 1328
XhoI C'TCGA_G
Cuts at: 0 6124 6204
Size: 6124 80
XmaI C'CCGG_G
Cuts at: 0 111 6204
Size: 111 6093
XmnI GAAnn'nnTTC
Cuts at: 0 1261 1386 6204
Size: 1261 125 4818
Fragments arranged by size:
4818 1261 125
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuII SacI SalI
SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BmtI
BsiWI BssHII BssSI BstAPI BstBI BstEII ClaI CspCI
DraIII FseI FspAI KpnI NheI NotI NruI PacI
PasI PmeI PsrI PvuI RsrII SacII SanDI SbfI
SfiI SgfI SgrAI SnaBI SpeI SwaI ZraI