(Circular) (Six-base) MAPSORT of: pFN21ASDA1051.seq Check: 2497 from: 1 to: 5800 pFN21ASDA1051 5800 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2535 3143 1937 Size: 598 608 4594 Fragments arranged by size: 4594 608 598 Acc65I G'GTAC_C Cuts at: 2931 3003 3019 3121 2931 Size: 72 16 102 5610 Fragments arranged by size: 5610 102 72 16 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3863 5247 5515 1632 Size: 2231 1384 268 1917 Fragments arranged by size: 2231 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 2605 4699 5676 1203 Size: 1402 2094 977 1327 Fragments arranged by size: 2094 1402 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2697 2729 2697 Size: 32 5768 AlwNI CAG_nnn'CTG Cuts at: 3543 5115 3543 Size: 1572 4228 ApaI G_GGCC'C Cuts at: 2201 2201 Size: 5800 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 2130 2815 2852 3109 3335 3487 5596 1493 Size: 637 685 37 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 685 637 257 226 152 37 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3125 1870 Size: 73 1182 4545 Fragments arranged by size: 4545 1182 73 AvrII C'CTAG_G Cuts at: 2299 2299 Size: 5800 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2987 3011 3020 3044 2987 Size: 24 9 24 5743 Fragments arranged by size: 5743 24 24 9 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2181 2931 3003 3019 3121 Size: 325 217 627 394 750 72 16 102 Cuts at: 3121 4576 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 750 627 394 325 217 102 72 16 BanII G_rGCy'C Cuts at: 1391 2201 2710 2767 3119 1391 Size: 810 509 57 352 4072 Fragments arranged by size: 4072 810 509 352 57 BbeI G_GCGC'C Cuts at: 2185 2185 Size: 5800 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 928 Size: 971 930 3899 Fragments arranged by size: 3899 971 930 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2066 2099 2100 2133 3991 4025 1903 Size: 34 129 33 1 33 1858 34 3678 Fragments arranged by size: 3678 1858 129 34 34 33 33 1 BciVI GTATCCnnnnn_n' Cuts at: 2459 2805 2867 3692 4902 2459 Size: 346 62 825 1210 3357 Fragments arranged by size: 3357 1210 825 346 62 BclI T'GATC_A Cuts at: 1548 2104 2982 1548 Size: 556 878 4366 Fragments arranged by size: 4366 878 556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4411 136 Size: 107 122 71 631 605 2739 1525 Fragments arranged by size: 2739 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 2315 3217 2315 Size: 902 4898 Bme1580I G_kGCm'C Cuts at: 1790 2201 3861 5017 1790 Size: 411 1660 1156 2573 Fragments arranged by size: 2573 1660 1156 411 BmgBI CAC'GTC Cuts at: 1318 2533 1318 Size: 1215 4585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2195 2592 2909 Size: 416 53 83 399 24 778 397 317 Cuts at: 2909 2930 3531 4489 442 Size: 21 601 958 1753 Fragments arranged by size: 1753 958 778 601 416 399 397 317 83 53 24 21 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2012 2502 2601 2702 4460 1239 Size: 773 490 99 101 1758 2579 Fragments arranged by size: 2579 1758 773 490 101 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3097 3591 493 Size: 711 1893 494 2702 Fragments arranged by size: 2702 1893 711 494 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2182 3607 3989 4624 Size: 53 83 186 936 649 1425 382 635 Cuts at: 4624 275 Size: 1451 Fragments arranged by size: 1451 1425 936 649 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3016 4233 4631 4905 5052 5463 3016 Size: 1217 398 274 147 411 3353 Fragments arranged by size: 3353 1217 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2263 2293 2403 2433 2697 Size: 30 165 30 860 30 110 30 264 Cuts at: 2697 2727 1178 Size: 30 4251 Fragments arranged by size: 4251 860 264 165 110 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5800 BseYI C'CCAG_C Cuts at: 5003 5499 5003 Size: 496 5304 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5227 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2321 2348 2532 2587 2767 3861 3946 5017 2321 Size: 27 184 55 180 1094 85 1071 3104 Fragments arranged by size: 3104 1094 1071 184 180 85 55 27 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 BsmBI CGTCTCn'nnnn_ Cuts at: 2940 2940 Size: 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2321 2348 2532 2587 2710 2767 Size: 399 411 120 27 184 55 123 57 Cuts at: 2767 3119 3861 3946 5017 1391 Size: 352 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 352 184 123 120 85 57 55 27 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3155 844 Size: 2311 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 2511 96 Size: 2415 3385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 2007 4604 2007 Size: 2597 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstYI r'GATC_y Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795 Size: 1158 367 385 17 1452 86 12 346 Cuts at: 5795 1972 Size: 1977 Fragments arranged by size: 1977 1452 1158 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5248 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2400 487 Size: 683 56 1174 3887 Fragments arranged by size: 3887 1174 683 56 BtsI GCAGTG_nn' Cuts at: 2386 3316 4110 4130 2386 Size: 930 794 20 4056 Fragments arranged by size: 4056 930 794 20 ClaI AT'CG_AT Cuts at: 1554 2076 1554 Size: 522 5278 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2145 2180 529 Size: 35 1581 35 4149 Fragments arranged by size: 4149 1581 35 35 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2153 3247 4136 Size: 54 1425 50 275 18 323 1094 889 Cuts at: 4136 5765 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1094 889 323 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2646 2720 3730 1353 Size: 1293 74 1010 3423 Fragments arranged by size: 3423 1293 1010 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2765 2765 Size: 5800 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2012 2502 2601 2702 3863 4460 5247 Size: 393 380 490 99 101 1161 597 787 Cuts at: 5247 5515 1239 Size: 268 1524 Fragments arranged by size: 1524 1161 787 597 490 393 380 268 101 99 EcoRI G'AATT_C Cuts at: 2130 2852 3109 3487 2130 Size: 722 257 378 4443 Fragments arranged by size: 4443 722 378 257 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2185 4947 1678 Size: 507 2762 2531 Fragments arranged by size: 2762 2531 507 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2243 2263 2275 Size: 32 135 32 486 32 153 20 12 Cuts at: 2275 2295 2343 2375 2386 2418 4441 4473 4515 Size: 20 48 32 11 32 2023 32 42 Cuts at: 4515 4547 1373 Size: 32 2626 Fragments arranged by size: 2626 2023 486 153 135 48 42 32 32 32 32 32 32 32 20 20 12 11 HincII GTy'rAC Cuts at: 669 1657 1938 2233 2557 3144 3390 669 Size: 988 281 295 324 587 246 3079 Fragments arranged by size: 3079 988 587 324 295 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KasI G'GCGC_C Cuts at: 2181 2181 Size: 5800 KpnI G_GTAC'C Cuts at: 2935 3007 3023 3125 2935 Size: 72 16 102 5610 Fragments arranged by size: 5610 102 72 16 MfeI C'AATT_G Cuts at: 2172 3399 2172 Size: 1227 4573 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2711 4914 5098 1305 Size: 1406 2203 184 2007 Fragments arranged by size: 2203 2007 1406 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2155 10 Size: 54 1425 343 323 3655 Fragments arranged by size: 3655 1425 343 323 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 2570 3758 4117 4276 518 Size: 648 946 458 1188 359 159 2042 Fragments arranged by size: 2042 1188 946 648 458 359 159 MspA1I CmG'CkG Cuts at: 1606 3216 3893 5041 5286 1606 Size: 1610 677 1148 245 2120 Fragments arranged by size: 2120 1610 1148 677 245 NarI GG'CG_CC Cuts at: 2182 2182 Size: 5800 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5248 NdeI CA'TA_TG Cuts at: 387 2893 387 Size: 2506 3294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 2609 3158 4703 1132 Size: 97 1380 549 1545 2229 Fragments arranged by size: 2229 1545 1380 549 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 2605 4699 2605 Size: 2094 3706 PflMI CCAn_nnn'nTGG Cuts at: 1108 2462 1108 Size: 1354 4446 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2697 2729 3869 3901 5382 5414 2697 Size: 32 1140 32 1481 32 3083 Fragments arranged by size: 3083 1481 1140 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PspOMI G'GGCC_C Cuts at: 2197 2197 Size: 5800 PstI C_TGCA'G Cuts at: 830 3152 830 Size: 2322 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 SacI G_AGCT'C Cuts at: 2767 2767 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SapI GCTCTTCn'nnn_ Cuts at: 2646 2646 Size: 5800 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 3148 4283 4964 5155 826 Size: 219 2103 1135 681 191 1471 Fragments arranged by size: 2103 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2183 2183 Size: 5800 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3904 4805 5067 820 Size: 197 926 1961 901 262 1553 Fragments arranged by size: 1961 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3097 3591 493 Size: 2604 494 2702 Fragments arranged by size: 2702 2604 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5800 StyI C'CwwG_G Cuts at: 513 1065 2299 3239 513 Size: 552 1234 940 3074 Fragments arranged by size: 3074 1234 940 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3827 3844 3997 4182 4627 325 Size: 1255 2247 17 153 185 445 1498 Fragments arranged by size: 2247 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2511 4046 5758 Size: 274 80 33 51 1443 534 1535 1712 Cuts at: 5758 96 Size: 138 Fragments arranged by size: 1712 1535 1443 534 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2311 2343 2403 2435 2977 3009 5760 5792 2311 Size: 32 60 32 542 32 2751 32 2319 Fragments arranged by size: 2751 2319 542 60 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2275 1316 Size: 959 4841 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI