(Circular) (Six-base) MAPSORT of: pFN21ASDA1048.seq Check: 5478 from: 1 to: 7318 pFN21ASDA1048 7318 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5128 278 Size: 53 83 186 936 3592 2468 Fragments arranged by size: 3592 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4100 4661 1937 Size: 2163 561 4594 Fragments arranged by size: 4594 2163 561 Acc65I G'GTAC_C Cuts at: 4639 4639 Size: 7318 AclI AA'CG_TT Cuts at: 1563 4077 5445 5818 1563 Size: 2514 1368 373 3063 Fragments arranged by size: 3063 2514 1368 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 4074 4167 4497 5381 6765 7033 1632 Size: 941 1501 93 330 884 1384 268 1917 Fragments arranged by size: 1917 1501 1384 941 884 330 268 93 AfeI AGC'GCT Cuts at: 3183 3183 Size: 7318 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7121 AflIII A'CryG_T Cuts at: 1203 6217 7194 1203 Size: 5014 977 1327 Fragments arranged by size: 5014 1327 977 AgeI A'CCGG_T Cuts at: 6149 6149 Size: 7318 AhdI GACnn_n'nnGTC Cuts at: 2156 6047 2156 Size: 3891 3427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7318 AlwNI CAG_nnn'CTG Cuts at: 2446 2915 3304 3379 3782 5061 6633 2446 Size: 469 389 75 403 1279 1572 3131 Fragments arranged by size: 3131 1572 1279 469 403 389 75 ApaLI G'TGCA_C Cuts at: 5375 6531 5375 Size: 1156 6162 ApoI r'AATT_y Cuts at: 1493 2624 3610 4627 4853 5005 7114 1493 Size: 1131 986 1017 226 152 2109 1697 Fragments arranged by size: 2109 1697 1131 1017 986 226 152 AseI AT'TA_AT Cuts at: 160 1032 5872 160 Size: 872 4840 1606 Fragments arranged by size: 4840 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7318 AvaI C'yCGr_G Cuts at: 1870 1943 2104 4196 4643 1870 Size: 73 161 2092 447 4545 Fragments arranged by size: 4545 2092 447 161 73 BamHI G'GATC_C Cuts at: 3233 4648 5015 3233 Size: 1415 367 5536 Fragments arranged by size: 5536 1415 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4639 6094 618 Size: 325 217 627 2852 1455 1842 Fragments arranged by size: 2852 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2075 2764 4637 1391 Size: 684 689 1873 4072 Fragments arranged by size: 4072 1873 689 684 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2128 928 Size: 971 229 6118 Fragments arranged by size: 6118 971 229 BbvCI CC'TCA_GC Cuts at: 4005 4005 Size: 7318 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5509 5543 1903 Size: 34 3572 34 3678 Fragments arranged by size: 3678 3572 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5210 6420 5210 Size: 1210 6108 BclI T'GATC_A Cuts at: 1548 1548 Size: 7318 BfrBI ATG'CAT Cuts at: 4522 4522 Size: 7318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3044 5929 136 Size: 107 122 71 631 605 1372 2885 1525 Fragments arranged by size: 2885 1525 1372 631 605 122 107 71 BglII A'GATC_T Cuts at: 7313 7313 Size: 7318 BlpI GC'TnA_GC Cuts at: 4213 4735 4213 Size: 522 6796 Bme1580I G_kGCm'C Cuts at: 1790 2322 2981 3918 5379 6535 1790 Size: 532 659 937 1461 1156 2573 Fragments arranged by size: 2573 1461 1156 937 659 532 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7318 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3803 5049 6007 Size: 416 53 83 399 24 2386 1246 958 Cuts at: 6007 442 Size: 1753 Fragments arranged by size: 2386 1753 1246 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2065 2258 2462 3974 5978 1239 Size: 826 193 204 1512 2004 2579 Fragments arranged by size: 2579 2004 1512 826 204 193 Bpu10I CC'TnA_GC Cuts at: 3387 4005 3387 Size: 618 6700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2856 2951 3413 5443 6308 6606 2856 Size: 95 462 2030 865 298 3568 Fragments arranged by size: 3568 2030 865 462 298 95 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5981 882 Size: 617 4482 2219 Fragments arranged by size: 4482 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4104 4615 5109 493 Size: 711 2900 511 494 2702 Fragments arranged by size: 2900 2702 711 511 494 BsaBI GATnn'nnATC Cuts at: 7312 7312 Size: 7318 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3356 5125 5507 6142 Size: 53 83 186 936 1823 1769 382 635 Cuts at: 6142 275 Size: 1451 Fragments arranged by size: 1823 1769 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5751 6149 6423 6570 6981 5751 Size: 398 274 147 411 6088 Fragments arranged by size: 6088 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3829 3859 4027 4057 1178 Size: 30 165 30 2426 30 168 30 4439 Fragments arranged by size: 4439 2426 168 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2103 3327 4047 1186 Size: 917 1224 720 4457 Fragments arranged by size: 4457 1224 917 720 BseYI C'CCAG_C Cuts at: 3063 3137 3293 3405 3543 3747 3911 3981 6521 Size: 74 156 112 138 204 164 70 2540 Cuts at: 6521 7017 3063 Size: 496 3364 Fragments arranged by size: 3364 2540 496 204 164 156 138 112 74 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3969 1163 Size: 573 2233 4512 Fragments arranged by size: 4512 2233 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4768 5529 6557 1540 Size: 275 186 2767 761 1028 2301 Fragments arranged by size: 2767 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2391 2478 2704 5379 5464 6535 2391 Size: 87 226 2675 85 1071 3174 Fragments arranged by size: 3174 2675 1071 226 87 85 BsmI GAATG_Cn' Cuts at: 4829 4922 4829 Size: 93 7225 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2322 2391 2478 2704 2764 2981 Size: 399 285 247 69 87 226 60 217 Cuts at: 2981 3918 4637 5379 5464 6535 1391 Size: 937 719 742 85 1071 2174 Fragments arranged by size: 2174 1071 937 742 719 399 285 247 226 217 87 85 69 60 BspEI T'CCGG_A Cuts at: 6981 6981 Size: 7318 BspHI T'CATG_A Cuts at: 5207 6937 5207 Size: 1730 5588 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2146 4673 844 Size: 1302 2527 3489 Fragments arranged by size: 3489 2527 1302 BsrBI CCG'CTC Cuts at: 1394 1823 3386 5205 1394 Size: 429 1563 1819 3507 Fragments arranged by size: 3507 1819 1563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2227 5813 5987 65 Size: 1183 979 3586 174 1396 Fragments arranged by size: 3586 1396 1183 979 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3475 5962 6149 1700 Size: 110 1665 2487 187 2869 Fragments arranged by size: 2869 2487 1665 187 110 BsrGI T'GTAC_A Cuts at: 96 2721 2992 96 Size: 2625 271 4422 Fragments arranged by size: 4422 2625 271 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7318 BssSI C'ACGA_G Cuts at: 5378 6390 5378 Size: 1012 6306 BstAPI GCAn_nnn'nTGC Cuts at: 794 3604 794 Size: 2810 4508 BstBI TT'CG_AA Cuts at: 2007 6122 2007 Size: 4115 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7318 BstXI CCAn_nnnn'nTGG Cuts at: 3911 3911 Size: 7318 BstYI r'GATC_y Cuts at: 1972 2308 3085 3233 3872 4456 4648 5015 5400 Size: 336 777 148 639 584 192 367 385 Cuts at: 5400 5417 6869 6955 6967 7313 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 777 639 584 385 367 346 336 192 148 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3222 3222 Size: 7318 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6766 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6579 Fragments arranged by size: 6579 683 56 BtsI GCAGTG_nn' Cuts at: 2122 2881 4834 5628 5648 2122 Size: 759 1953 794 20 3792 Fragments arranged by size: 3792 1953 794 759 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7318 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7283 DraI TTT'AAA Cuts at: 4622 4622 Size: 7318 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6325 809 Size: 507 5009 1802 Fragments arranged by size: 5009 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2416 3663 4765 Size: 54 1425 50 275 18 586 1247 1102 Cuts at: 4765 5654 7283 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1247 1102 889 586 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4765 1537 Size: 275 2953 4090 Fragments arranged by size: 4090 2953 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2125 3191 3428 4371 4431 5248 1353 Size: 772 1066 237 943 60 817 3423 Fragments arranged by size: 3423 1066 943 817 772 237 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2195 5905 6279 6425 387 Size: 1064 138 606 3710 374 146 1280 Fragments arranged by size: 3710 1280 1064 606 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2258 2462 2573 3974 4074 4167 Size: 393 433 193 204 111 1401 100 93 Cuts at: 4167 4497 5381 5978 6765 7033 1239 Size: 330 884 597 787 268 1524 Fragments arranged by size: 1524 1401 884 787 597 433 393 330 268 204 193 111 100 93 EcoNI CCTnn'n_nnAGG Cuts at: 4092 4092 Size: 7318 EcoO109I rG'GnC_Cy Cuts at: 2548 3961 2548 Size: 1413 5905 EcoRI G'AATT_C Cuts at: 4627 5005 4627 Size: 378 6940 FspI TGC'GCA Cuts at: 5824 5824 Size: 7318 HaeII r_GCGC'y Cuts at: 1678 2446 2616 3185 6465 1678 Size: 768 170 569 3280 2531 Fragments arranged by size: 3280 2531 768 569 170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2142 2174 4115 4147 5959 Size: 32 135 32 570 32 1941 32 1812 Cuts at: 5959 5991 6033 6065 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1941 1812 570 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2257 2716 4564 4662 4908 669 Size: 988 281 319 459 1848 98 246 3079 Fragments arranged by size: 3079 1848 988 459 319 281 246 98 HindIII A'AGCT_T Cuts at: 748 3017 4175 4202 748 Size: 2269 1158 27 3864 Fragments arranged by size: 3864 2269 1158 27 HpaI GTT'AAC Cuts at: 4564 4908 4564 Size: 344 6974 KpnI G_GTAC'C Cuts at: 4643 4643 Size: 7318 MfeI C'AATT_G Cuts at: 3111 3555 4917 3111 Size: 444 1362 5512 Fragments arranged by size: 5512 1362 444 MluI A'CGCG_T Cuts at: 7194 7194 Size: 7318 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2883 3850 6432 6616 1305 Size: 1578 967 2582 184 2007 Fragments arranged by size: 2582 2007 1578 967 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2418 3665 10 Size: 54 1425 343 586 1247 3663 Fragments arranged by size: 3663 1425 1247 586 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 5276 5635 5794 518 Size: 648 1230 2880 359 159 2042 Fragments arranged by size: 2880 2042 1230 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 3050 3661 3688 3761 3985 3998 4004 Size: 479 965 611 27 73 224 13 6 Cuts at: 4004 4145 4217 4734 5411 6559 6804 1606 Size: 141 72 517 677 1148 245 2120 Fragments arranged by size: 2120 1148 965 677 611 517 479 245 224 141 73 72 27 13 6 NaeI GCC'GGC Cuts at: 3477 3477 Size: 7318 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6766 NdeI CA'TA_TG Cuts at: 387 387 Size: 7318 NgoMIV G'CCGG_C Cuts at: 3475 3475 Size: 7318 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7318 NotI GC'GGCC_GC Cuts at: 4765 4765 Size: 7318 NsiI A_TGCA'T Cuts at: 4524 4524 Size: 7318 NspI r_CATG'y Cuts at: 1132 1229 2251 2881 4676 6221 1132 Size: 97 1022 630 1795 1545 2229 Fragments arranged by size: 2229 1795 1545 1022 630 97 PasI CC'CwG_GG Cuts at: 1348 3373 4573 1348 Size: 2025 1200 4093 Fragments arranged by size: 4093 2025 1200 PciI A'CATG_T Cuts at: 6217 6217 Size: 7318 PflMI CCAn_nnn'nTGG Cuts at: 1108 4573 1108 Size: 3465 3853 PmeI GTTT'AAAC Cuts at: 4622 4622 Size: 7318 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5387 5419 6900 6932 5387 Size: 32 1481 32 5773 Fragments arranged by size: 5773 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 3961 2548 Size: 1413 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7318 PsiI TTA'TAA Cuts at: 4083 4888 4083 Size: 805 6513 PstI C_TGCA'G Cuts at: 830 3232 3766 4003 4670 830 Size: 2402 534 237 667 3478 Fragments arranged by size: 3478 2402 667 534 237 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7318 PvuII CAG'CTG Cuts at: 3050 3661 3688 3761 3985 3998 4004 4145 4217 Size: 611 27 73 224 13 6 141 72 Cuts at: 4217 3050 Size: 6151 Fragments arranged by size: 6151 611 224 141 73 72 27 13 6 SalI G'TCGA_C Cuts at: 1936 4660 1936 Size: 2724 4594 SanDI GG'GwC_CC Cuts at: 3961 3961 Size: 7318 SbfI CC_TGCA'GG Cuts at: 4670 4670 Size: 7318 ScaI AGT'ACT Cuts at: 5566 7278 5566 Size: 1712 5606 SexAI A'CCwGG_T Cuts at: 2937 2937 Size: 7318 SfcI C'TryA_G Cuts at: 826 1045 3228 3762 3999 4666 5801 6482 6673 Size: 219 2183 534 237 667 1135 681 191 Cuts at: 6673 826 Size: 1471 Fragments arranged by size: 2183 1471 1135 681 667 534 237 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7318 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7318 SmaI CCC'GGG Cuts at: 1872 4645 1872 Size: 2773 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2871 2966 3428 5422 6323 6585 Size: 197 926 928 95 462 1994 901 262 Cuts at: 6585 820 Size: 1553 Fragments arranged by size: 1994 1553 928 926 901 462 262 197 95 SnaBI TAC'GTA Cuts at: 493 4615 5109 493 Size: 4122 494 2702 Fragments arranged by size: 4122 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7318 SphI G_CATG'C Cuts at: 1132 4676 1132 Size: 3544 3774 SspI AAT'ATT Cuts at: 5 52 2329 5242 5 Size: 47 2277 2913 2081 Fragments arranged by size: 2913 2277 2081 47 StuI AGG'CCT Cuts at: 1517 3130 1517 Size: 1613 5705 StyI C'CwwG_G Cuts at: 513 1065 3580 4249 4602 4757 513 Size: 552 2515 669 353 155 3074 Fragments arranged by size: 3074 2515 669 552 353 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2595 3307 5345 5362 5515 5700 6145 Size: 1255 1015 712 2038 17 153 185 445 Cuts at: 6145 325 Size: 1498 Fragments arranged by size: 2038 1498 1255 1015 712 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2721 2992 3529 Size: 274 80 33 51 1443 744 271 537 Cuts at: 3529 5564 7276 96 Size: 2035 1712 138 Fragments arranged by size: 2035 1712 1443 744 537 274 271 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2218 2250 7278 7310 2218 Size: 32 5028 32 2226 Fragments arranged by size: 5028 2226 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7318 XbaI T'CTAG_A Cuts at: 4654 4654 Size: 7318 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7318 XmaI C'CCGG_G Cuts at: 1870 4643 1870 Size: 2773 4545 XmnI GAAnn'nnTTC Cuts at: 2033 5447 2033 Size: 3414 3904 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5126 276 Size: 53 83 186 936 3592 2468 Fragments arranged by size: 3592 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI