(Circular) (Six-base) MAPSORT of: pFN21ASDA1045.seq Check: 8362 from: 1 to: 5929 pFN21ASDA1045 5929 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2050 2050 Size: 5929 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3739 278 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3272 1937 Size: 1335 4594 Acc65I G'GTAC_C Cuts at: 3250 3250 Size: 5929 AclI AA'CG_TT Cuts at: 1563 4056 4429 1563 Size: 2493 373 3063 Fragments arranged by size: 3063 2493 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3230 3992 5376 5644 1632 Size: 1598 762 1384 268 1917 Fragments arranged by size: 1917 1598 1384 762 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5732 AflIII A'CryG_T Cuts at: 1203 4828 5805 1203 Size: 3625 977 1327 Fragments arranged by size: 3625 1327 977 AgeI A'CCGG_T Cuts at: 4760 4760 Size: 5929 AhdI GACnn_n'nnGTC Cuts at: 2827 4658 2827 Size: 1831 4098 AleI CACnn'nnGTG Cuts at: 1166 2583 1166 Size: 1417 4512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5929 AlwNI CAG_nnn'CTG Cuts at: 2469 3672 5244 2469 Size: 1203 1572 3154 Fragments arranged by size: 3154 1572 1203 ApaI G_GGCC'C Cuts at: 2740 2980 2740 Size: 240 5689 ApaLI G'TGCA_C Cuts at: 3986 5142 3986 Size: 1156 4773 ApoI r'AATT_y Cuts at: 1493 3238 3464 3616 5725 1493 Size: 1745 226 152 2109 1697 Fragments arranged by size: 2109 1745 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4483 160 Size: 872 3451 1606 Fragments arranged by size: 3451 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 AvaI C'yCGr_G Cuts at: 1870 1943 2916 2934 3254 1870 Size: 73 973 18 320 4545 Fragments arranged by size: 4545 973 320 73 18 BamHI G'GATC_C Cuts at: 3259 3626 3259 Size: 367 5562 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3250 4705 618 Size: 325 217 627 1463 1455 1842 Fragments arranged by size: 1842 1463 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2174 2391 2740 2917 2980 3248 1391 Size: 783 217 349 177 63 268 4072 Fragments arranged by size: 4072 783 349 268 217 177 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4958 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2199 2233 4120 4154 1903 Size: 34 262 34 1887 34 3678 Fragments arranged by size: 3678 1887 262 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3215 3821 5031 3215 Size: 606 1210 4113 Fragments arranged by size: 4113 1210 606 BclI T'GATC_A Cuts at: 1548 1548 Size: 5929 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2508 2930 4540 Size: 107 122 71 631 605 836 422 1610 Cuts at: 4540 136 Size: 1525 Fragments arranged by size: 1610 1525 836 631 605 422 122 107 71 BglII A'GATC_T Cuts at: 5924 5924 Size: 5929 BlpI GC'TnA_GC Cuts at: 3346 3346 Size: 5929 Bme1580I G_kGCm'C Cuts at: 1790 2740 2980 3990 5146 1790 Size: 950 240 1010 1156 2573 Fragments arranged by size: 2573 1156 1010 950 240 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5929 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 4618 442 Size: 416 53 83 399 24 2243 958 1753 Fragments arranged by size: 2243 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2355 2405 2590 2702 4589 1239 Size: 1116 50 185 112 1887 2579 Fragments arranged by size: 2579 1887 1116 185 112 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4054 4919 5217 4054 Size: 865 298 4766 Fragments arranged by size: 4766 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2630 4592 882 Size: 617 1131 1962 2219 Fragments arranged by size: 2219 1962 1131 617 BsaAI yAC'GTr Cuts at: 493 1204 3226 3720 493 Size: 711 2022 494 2702 Fragments arranged by size: 2702 2022 711 494 BsaBI GATnn'nnATC Cuts at: 5923 5923 Size: 5929 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3736 4118 4753 275 Size: 53 83 186 936 2203 382 635 1451 Fragments arranged by size: 2203 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4362 4760 5034 5181 5592 4362 Size: 398 274 147 411 4699 Fragments arranged by size: 4699 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2606 2636 1178 Size: 30 165 30 1203 30 4471 Fragments arranged by size: 4471 1203 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2309 2627 2762 1186 Size: 1021 102 318 135 4353 Fragments arranged by size: 4353 1021 318 135 102 BseYI C'CCAG_C Cuts at: 2979 3067 5132 5628 2979 Size: 88 2065 496 3280 Fragments arranged by size: 3280 2065 496 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2084 2455 2482 2550 1163 Size: 573 348 371 27 68 4542 Fragments arranged by size: 4542 573 371 348 68 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2248 3379 4140 5168 1540 Size: 275 186 247 1131 761 1028 2301 Fragments arranged by size: 2301 1131 1028 761 275 247 186 BsiHKAI G_wGCw'C Cuts at: 2951 3990 4075 5146 2951 Size: 1039 85 1071 3734 Fragments arranged by size: 3734 1071 1039 85 BsmI GAATG_Cn' Cuts at: 3440 3533 3440 Size: 93 5836 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2391 2740 2917 2951 2980 3248 Size: 399 384 217 349 177 34 29 268 Cuts at: 3248 3990 4075 5146 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 384 349 268 217 177 85 34 29 BspEI T'CCGG_A Cuts at: 5592 5592 Size: 5929 BspHI T'CATG_A Cuts at: 3818 5548 3818 Size: 1730 4199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 3284 844 Size: 1206 1234 3489 Fragments arranged by size: 3489 1234 1206 BsrBI CCG'CTC Cuts at: 1394 1823 2263 2905 3816 1394 Size: 429 440 642 911 3507 Fragments arranged by size: 3507 911 642 440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4424 4598 65 Size: 1183 3176 174 1396 Fragments arranged by size: 3176 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 4573 4760 1700 Size: 110 433 2330 187 2869 Fragments arranged by size: 2869 2330 433 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5929 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5929 BssSI C'ACGA_G Cuts at: 3989 5001 3989 Size: 1012 4917 BstAPI GCAn_nnn'nTGC Cuts at: 794 2469 2860 794 Size: 1675 391 3863 Fragments arranged by size: 3863 1675 391 BstBI TT'CG_AA Cuts at: 2007 4733 2007 Size: 2726 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5929 BstXI CCAn_nnnn'nTGG Cuts at: 3133 3133 Size: 5929 BstYI r'GATC_y Cuts at: 1972 3259 3626 4011 4028 5480 5566 5578 5924 Size: 1287 367 385 17 1452 86 12 346 Cuts at: 5924 1972 Size: 1977 Fragments arranged by size: 1977 1452 1287 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2292 2292 Size: 5929 BtgI C'CryG_G Cuts at: 513 1065 2022 2155 513 Size: 552 957 133 4287 Fragments arranged by size: 4287 957 552 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3037 487 Size: 683 56 1811 3379 Fragments arranged by size: 3379 1811 683 56 BtsI GCAGTG_nn' Cuts at: 2947 3031 3445 4239 4259 2947 Size: 84 414 794 20 4617 Fragments arranged by size: 4617 794 414 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5929 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5894 DraI TTT'AAA Cuts at: 3233 3233 Size: 5929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4936 809 Size: 507 3620 1802 Fragments arranged by size: 3620 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 3376 4265 Size: 54 1425 50 275 18 415 1131 889 Cuts at: 4265 5894 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1131 889 415 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2245 3376 1537 Size: 275 433 1131 4090 Fragments arranged by size: 4090 1131 433 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 2459 2751 3859 1353 Size: 873 233 292 1108 3423 Fragments arranged by size: 3423 1108 873 292 233 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2327 4516 4890 5036 387 Size: 1064 138 738 2189 374 146 1280 Fragments arranged by size: 2189 1280 1064 738 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2355 2405 2590 2702 3230 3992 4589 Size: 393 723 50 185 112 528 762 597 Cuts at: 4589 5376 5644 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 762 723 597 528 393 268 185 112 50 EcoNI CCTnn'n_nnAGG Cuts at: 2744 2744 Size: 5929 EcoO109I rG'GnC_Cy Cuts at: 2148 2736 2737 3047 2148 Size: 588 1 310 5030 Fragments arranged by size: 5030 588 310 1 EcoRI G'AATT_C Cuts at: 3238 3616 3238 Size: 378 5551 FspI TGC'GCA Cuts at: 4435 4435 Size: 5929 HaeII r_GCGC'y Cuts at: 1678 5076 1678 Size: 3398 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2813 2845 3107 3139 4570 Size: 32 135 32 1241 32 262 32 1431 Cuts at: 4570 4602 4644 4676 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1431 1241 262 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2407 3273 3519 669 Size: 988 281 469 866 246 3079 Fragments arranged by size: 3079 988 866 469 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5929 HpaI GTT'AAC Cuts at: 3519 3519 Size: 5929 KpnI G_GTAC'C Cuts at: 3254 3254 Size: 5929 MfeI C'AATT_G Cuts at: 3528 3528 Size: 5929 MluI A'CGCG_T Cuts at: 5805 5805 Size: 5929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2149 5043 5227 1305 Size: 844 2894 184 2007 Fragments arranged by size: 2894 2007 844 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4107 Fragments arranged by size: 4107 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2583 3887 4246 4405 518 Size: 648 861 556 1304 359 159 2042 Fragments arranged by size: 2042 1304 861 648 556 359 159 MspA1I CmG'CkG Cuts at: 1606 2444 2534 2647 3345 4022 5170 5415 1606 Size: 838 90 113 698 677 1148 245 2120 Fragments arranged by size: 2120 1148 838 698 677 245 113 90 NaeI GCC'GGC Cuts at: 2245 2245 Size: 5929 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4420 Fragments arranged by size: 4420 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5929 NgoMIV G'CCGG_C Cuts at: 2243 2243 Size: 5929 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5929 NotI GC'GGCC_GC Cuts at: 3376 3376 Size: 5929 NspI r_CATG'y Cuts at: 1132 1229 3287 4832 1132 Size: 97 2058 1545 2229 Fragments arranged by size: 2229 2058 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5929 PciI A'CATG_T Cuts at: 4828 4828 Size: 5929 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5929 PfoI T'CCnGG_A Cuts at: 2178 2226 2178 Size: 48 5881 PmeI GTTT'AAAC Cuts at: 3233 3233 Size: 5929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3998 4030 5511 5543 3998 Size: 32 1481 32 4384 Fragments arranged by size: 4384 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2148 3047 2148 Size: 899 5030 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5929 PsiI TTA'TAA Cuts at: 3499 3499 Size: 5929 PspOMI G'GGCC_C Cuts at: 2736 2976 2736 Size: 240 5689 PstI C_TGCA'G Cuts at: 830 2856 3026 3281 830 Size: 2026 170 255 3478 Fragments arranged by size: 3478 2026 255 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5929 PvuII CAG'CTG Cuts at: 2444 2444 Size: 5929 SalI G'TCGA_C Cuts at: 1936 3271 1936 Size: 1335 4594 SanDI GG'GwC_CC Cuts at: 2148 3047 2148 Size: 899 5030 SapI GCTCTTCn'nnn_ Cuts at: 2751 2751 Size: 5929 SbfI CC_TGCA'GG Cuts at: 3281 3281 Size: 5929 ScaI AGT'ACT Cuts at: 4177 5889 4177 Size: 1712 4217 SfcI C'TryA_G Cuts at: 826 1045 2163 2852 3022 3277 4412 5093 5284 Size: 219 1118 689 170 255 1135 681 191 Cuts at: 5284 826 Size: 1471 Fragments arranged by size: 1471 1135 1118 689 681 255 219 191 170 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5929 SmaI CCC'GGG Cuts at: 1872 3256 1872 Size: 1384 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4033 4934 5196 820 Size: 197 926 2090 901 262 1553 Fragments arranged by size: 2090 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3226 3720 493 Size: 2733 494 2702 Fragments arranged by size: 2733 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5929 SphI G_CATG'C Cuts at: 1132 3287 1132 Size: 2155 3774 SspI AAT'ATT Cuts at: 5 52 3853 5 Size: 47 3801 2081 Fragments arranged by size: 3801 2081 47 StuI AGG'CCT Cuts at: 1517 2116 1517 Size: 599 5330 StyI C'CwwG_G Cuts at: 513 1065 2022 2518 3368 513 Size: 552 957 496 850 3074 Fragments arranged by size: 3074 957 850 552 496 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3956 3973 4126 4311 4756 325 Size: 1255 2376 17 153 185 445 1498 Fragments arranged by size: 2376 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4175 5887 96 Size: 274 80 33 51 1443 2198 1712 138 Fragments arranged by size: 2198 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 5889 5921 2205 Size: 32 3652 32 2213 Fragments arranged by size: 3652 2213 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5929 XbaI T'CTAG_A Cuts at: 3265 3265 Size: 5929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5929 XmaI C'CCGG_G Cuts at: 1870 3254 1870 Size: 1384 4545 XmnI GAAnn'nnTTC Cuts at: 4058 4058 Size: 5929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3737 276 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI