Restriction map for
pFN21ASDA1045
(Linear) (Six-base) MAPSORT of: KIAA1045.seq Check: 7886 from: 1 to: 1200
KIAA1045.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 27 1200
Size: 27 1173
AhdI GACnn_n'nnGTC
Cuts at: 0 804 1200
Size: 804 396
AleI CACnn'nnGTG
Cuts at: 0 560 1200
Size: 560 640
AlwNI CAG_nnn'CTG
Cuts at: 0 446 1200
Size: 446 754
ApaI G_GGCC'C
Cuts at: 0 717 957 1200
Size: 717 240 243
Fragments arranged by size:
717 243 240
AvaI C'yCGr_G
Cuts at: 0 893 911 1200
Size: 893 18 289
Fragments arranged by size:
893 289 18
BanII G_rGCy'C
Cuts at: 0 151 368 717 894 957 1200
Size: 151 217 349 177 63 243
Fragments arranged by size:
349 243 217 177 151 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 176 210 1200
Size: 176 34 990
Fragments arranged by size:
990 176 34
BglI GCCn_nnn'nGGC
Cuts at: 0 485 907 1200
Size: 485 422 293
Fragments arranged by size:
485 422 293
Bme1580I G_kGCm'C
Cuts at: 0 717 957 1200
Size: 717 240 243
Fragments arranged by size:
717 243 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 382 567 679 1200
Size: 332 50 185 112 521
Fragments arranged by size:
521 332 185 112 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 607 1200
Size: 607 593
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 583 613 1200
Size: 583 30 587
Fragments arranged by size:
587 583 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 286 604 739 1200
Size: 184 102 318 135 461
Fragments arranged by size:
461 318 184 135 102
BseYI C'CCAG_C
Cuts at: 0 956 1044 1200
Size: 956 88 156
Fragments arranged by size:
956 156 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 432 459 527 1200
Size: 61 371 27 68 673
Fragments arranged by size:
673 371 68 61 27
BsiEI CG_ry'CG
Cuts at: 0 225 1200
Size: 225 975
BsiHKAI G_wGCw'C
Cuts at: 0 928 1200
Size: 928 272
Bsp1286I G_dGCh'C
Cuts at: 0 151 368 717 894 928 957 1200
Size: 151 217 349 177 34 29 243
Fragments arranged by size:
349 243 217 177 151 34 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 27 1200
Size: 27 1173
BsrBI CCG'CTC
Cuts at: 0 240 882 1200
Size: 240 642 318
Fragments arranged by size:
642 318 240
BsrFI r'CCGG_y
Cuts at: 0 220 1200
Size: 220 980
BstAPI GCAn_nnn'nTGC
Cuts at: 0 446 837 1200
Size: 446 391 363
Fragments arranged by size:
446 391 363
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1200
Size: 1110 90
Bsu36I CC'TnA_GG
Cuts at: 0 269 1200
Size: 269 931
BtgI C'CryG_G
Cuts at: 0 132 1200
Size: 132 1068
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1014 1200
Size: 1014 186
BtsI GCAGTG_nn'
Cuts at: 0 924 1008 1200
Size: 924 84 192
Fragments arranged by size:
924 192 84
EaeI y'GGCC_r
Cuts at: 0 222 1200
Size: 222 978
EagI C'GGCC_G
Cuts at: 0 222 1200
Size: 222 978
EarI CTCTTCn'nnn_
Cuts at: 0 203 436 728 1200
Size: 203 233 292 472
Fragments arranged by size:
472 292 233 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 304 1200
Size: 304 896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 382 567 679 1200
Size: 332 50 185 112 521
Fragments arranged by size:
521 332 185 112 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 721 1200
Size: 721 479
EcoO109I rG'GnC_Cy
Cuts at: 0 125 713 714 1024 1200
Size: 125 588 1 310 176
Fragments arranged by size:
588 310 176 125 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 790 822 1084 1116 1200
Size: 790 32 262 32 84
Fragments arranged by size:
790 262 84 32 32
HincII GTy'rAC
Cuts at: 0 384 1200
Size: 384 816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 1200
Size: 126 1074
MslI CAynn'nnrTG
Cuts at: 0 560 1200
Size: 560 640
MspA1I CmG'CkG
Cuts at: 0 421 511 624 1200
Size: 421 90 113 576
Fragments arranged by size:
576 421 113 90
NaeI GCC'GGC
Cuts at: 0 222 1200
Size: 222 978
NgoMIV G'CCGG_C
Cuts at: 0 220 1200
Size: 220 980
PfoI T'CCnGG_A
Cuts at: 0 155 203 1200
Size: 155 48 997
Fragments arranged by size:
997 155 48
PpuMI rG'GwC_Cy
Cuts at: 0 125 1024 1200
Size: 125 899 176
Fragments arranged by size:
899 176 125
PspOMI G'GGCC_C
Cuts at: 0 713 953 1200
Size: 713 240 247
Fragments arranged by size:
713 247 240
PstI C_TGCA'G
Cuts at: 0 833 1003 1200
Size: 833 170 197
Fragments arranged by size:
833 197 170
PvuII CAG'CTG
Cuts at: 0 421 1200
Size: 421 779
SanDI GG'GwC_CC
Cuts at: 0 125 1024 1200
Size: 125 899 176
Fragments arranged by size:
899 176 125
SapI GCTCTTCn'nnn_
Cuts at: 0 728 1200
Size: 728 472
SfcI C'TryA_G
Cuts at: 0 140 829 999 1200
Size: 140 689 170 201
Fragments arranged by size:
689 201 170 140
StuI AGG'CCT
Cuts at: 0 93 1200
Size: 93 1107
StyI C'CwwG_G
Cuts at: 0 495 1200
Size: 495 705
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 182 214 1200
Size: 182 32 986
Fragments arranged by size:
986 182 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 327 1200
Size: 327 873
Enzymes that do cut:
AarI AhdI AleI AlwNI ApaI AvaI BanII BcgI
BglI Bme1580I BpmI BsaI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MslI MspA1I
NaeI NgoMIV PfoI PpuMI PspOMI PstI PvuII SanDI
SapI SfcI StuI StyI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI ZraI