(Circular) (Six-base) MAPSORT of: pFN21ASDA1036.seq Check: 1698 from: 1 to: 5824 pFN21ASDA1036 5824 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2366 3117 3167 1937 Size: 429 751 50 4594 Fragments arranged by size: 4594 751 429 50 Acc65I G'GTAC_C Cuts at: 3102 3145 3102 Size: 43 5781 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3887 5271 5539 1632 Size: 2255 1384 268 1917 Fragments arranged by size: 2255 1917 1384 268 AfeI AGC'GCT Cuts at: 2402 2585 2402 Size: 183 5641 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 2260 4723 5700 1203 Size: 1057 2463 977 1327 Fragments arranged by size: 2463 1327 1057 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaI G_GGCC'C Cuts at: 2954 3007 2954 Size: 53 5771 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 2550 3133 3359 3511 5620 1493 Size: 1057 583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1057 583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 3052 3149 1870 Size: 73 1109 97 4545 Fragments arranged by size: 4545 1109 97 73 BamHI G'GATC_C Cuts at: 2304 3154 3521 2304 Size: 850 367 4607 Fragments arranged by size: 4607 850 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 3102 3145 4600 618 Size: 325 217 627 276 1039 43 1455 1842 Fragments arranged by size: 1842 1455 1039 627 325 276 217 43 BanII G_rGCy'C Cuts at: 1391 2739 2954 3007 3078 3143 1391 Size: 1348 215 53 71 65 4072 Fragments arranged by size: 4072 1348 215 71 65 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3071 928 Size: 971 1172 3681 Fragments arranged by size: 3681 1172 971 BbvCI CC'TCA_GC Cuts at: 2134 2134 Size: 5824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 2729 3241 2729 Size: 512 5312 Bme1580I G_kGCm'C Cuts at: 1790 2954 3007 3885 5041 1790 Size: 1164 53 878 1156 2573 Fragments arranged by size: 2573 1164 1156 878 53 BmgBI CAC'GTC Cuts at: 1318 2866 1318 Size: 1548 4276 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3001 3555 4513 Size: 416 53 83 399 24 1584 554 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1584 958 554 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2484 2484 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2600 2924 4484 1239 Size: 1361 324 1560 2579 Fragments arranged by size: 2579 1560 1361 324 Bpu10I CC'TnA_GC Cuts at: 2134 2379 2134 Size: 245 5579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2768 2915 3949 4814 5112 2768 Size: 147 1034 865 298 3480 Fragments arranged by size: 3480 1034 865 298 147 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2111 4487 882 Size: 617 612 2376 2219 Fragments arranged by size: 2376 2219 617 612 BsaAI yAC'GTr Cuts at: 493 1204 2263 2779 3121 3615 493 Size: 711 1059 516 342 494 2702 Fragments arranged by size: 2702 1059 711 516 494 342 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 3631 4013 4648 Size: 53 83 186 936 660 1438 382 635 Cuts at: 4648 275 Size: 1451 Fragments arranged by size: 1451 1438 936 660 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2973 4257 4655 4929 5076 5487 2973 Size: 1284 398 274 147 411 3310 Fragments arranged by size: 3310 1284 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2277 2307 2938 2968 1178 Size: 30 165 30 874 30 631 30 4034 Fragments arranged by size: 4034 874 631 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 1186 Size: 979 4845 BseYI C'CCAG_C Cuts at: 2045 2641 2674 2797 5027 5523 2045 Size: 596 33 123 2230 496 2346 Fragments arranged by size: 2346 2230 596 496 123 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2417 1163 Size: 573 681 4570 Fragments arranged by size: 4570 681 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2767 3274 4035 5063 1540 Size: 275 186 766 507 761 1028 2301 Fragments arranged by size: 2301 1028 766 761 507 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 2784 3885 3970 5041 2739 Size: 45 1101 85 1071 3522 Fragments arranged by size: 3522 1101 1071 85 45 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 BsmBI CGTCTCn'nnnn_ Cuts at: 2178 2178 Size: 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2739 2784 2954 3007 3078 3143 3885 Size: 399 949 45 170 53 71 65 742 Cuts at: 3885 3970 5041 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 949 742 399 170 85 71 65 53 45 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 2331 3179 844 Size: 1343 144 848 3489 Fragments arranged by size: 3489 1343 848 144 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4468 4655 1700 Size: 110 2658 187 2869 Fragments arranged by size: 2869 2658 187 110 BsrGI T'GTAC_A Cuts at: 96 2703 96 Size: 2607 3217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 2738 3884 4896 2738 Size: 1146 1012 3666 Fragments arranged by size: 3666 1146 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2857 794 Size: 2063 3761 BstBI TT'CG_AA Cuts at: 2007 4628 2007 Size: 2621 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2282 2282 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2271 2304 2331 3108 3154 3521 3906 3923 Size: 299 33 27 777 46 367 385 17 Cuts at: 3923 5375 5461 5473 5819 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 777 385 367 346 299 86 46 33 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2308 513 Size: 552 1243 4029 Fragments arranged by size: 4029 1243 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2898 487 Size: 683 56 1672 3413 Fragments arranged by size: 3413 1672 683 56 BtsI GCAGTG_nn' Cuts at: 3043 3340 4134 4154 3043 Size: 297 794 20 4713 Fragments arranged by size: 4713 794 297 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2197 2232 529 Size: 35 1633 35 4121 Fragments arranged by size: 4121 1633 35 35 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 2638 2830 2857 2638 Size: 192 27 5605 Fragments arranged by size: 5605 192 27 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2192 4831 809 Size: 507 876 2639 1802 Fragments arranged by size: 2639 1802 876 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2265 2490 Size: 54 1425 50 275 18 255 180 225 Cuts at: 2490 2764 3271 4160 5789 8 Size: 274 507 889 1629 43 Fragments arranged by size: 1629 1425 889 507 275 274 255 225 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2764 3271 1537 Size: 275 952 507 4090 Fragments arranged by size: 4090 952 507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 2166 2453 3754 1353 Size: 801 12 287 1301 3423 Fragments arranged by size: 3423 1301 801 287 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2618 4411 4785 4931 387 Size: 1064 138 1029 1793 374 146 1280 Fragments arranged by size: 1793 1280 1064 1029 374 146 138 EcoICRI GAG'CTC Cuts at: 2737 2737 Size: 5824 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2600 2924 3887 4484 5271 5539 1239 Size: 393 968 324 963 597 787 268 1524 Fragments arranged by size: 1524 968 963 787 597 393 324 268 EcoNI CCTnn'n_nnAGG Cuts at: 2245 2472 2492 2245 Size: 227 20 5577 Fragments arranged by size: 5577 227 20 EcoO109I rG'GnC_Cy Cuts at: 2176 2512 2695 2951 2176 Size: 336 183 256 5049 Fragments arranged by size: 5049 336 256 183 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2626 2626 Size: 5824 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2386 2404 2587 2875 2911 4971 1678 Size: 708 18 183 288 36 2060 2531 Fragments arranged by size: 2531 2060 708 288 183 36 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2938 2970 4465 4497 4539 Size: 32 135 32 1366 32 1495 32 42 Cuts at: 4539 4571 1373 Size: 32 2626 Fragments arranged by size: 2626 1495 1366 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2209 3168 3414 669 Size: 988 281 271 959 246 3079 Fragments arranged by size: 3079 988 959 281 271 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3106 3149 3106 Size: 43 5781 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2096 4938 5122 1305 Size: 791 2842 184 2007 Fragments arranged by size: 2842 2007 791 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2267 2492 10 Size: 54 1425 343 435 225 3342 Fragments arranged by size: 3342 1425 435 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2280 3782 4141 4300 518 Size: 648 1114 1502 359 159 2042 Fragments arranged by size: 2042 1502 1114 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2061 2081 2769 3001 3240 3917 5065 5310 Size: 455 20 688 232 239 677 1148 245 Cuts at: 5310 1606 Size: 2120 Fragments arranged by size: 2120 1148 688 677 455 245 239 232 20 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5272 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2572 2926 3182 4727 1132 Size: 97 1343 354 256 1545 2229 Fragments arranged by size: 2229 1545 1343 354 256 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PmlI CAC'GTG Cuts at: 2263 2779 2263 Size: 516 5308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 2695 2176 Size: 519 5305 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2950 3003 2950 Size: 53 5771 PstI C_TGCA'G Cuts at: 830 2421 3176 830 Size: 1591 755 3478 Fragments arranged by size: 3478 1591 755 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 SacI G_AGCT'C Cuts at: 2739 2739 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SapI GCTCTTCn'nnn_ Cuts at: 2453 2453 Size: 5824 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2417 3172 4307 4988 5179 826 Size: 219 1372 755 1135 681 191 1471 Fragments arranged by size: 1471 1372 1135 755 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2783 2930 3928 4829 5091 820 Size: 197 926 840 147 998 901 262 1553 Fragments arranged by size: 1553 998 926 901 840 262 197 147 SnaBI TAC'GTA Cuts at: 493 3121 3615 493 Size: 2628 494 2702 Fragments arranged by size: 2702 2628 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 2572 3182 1132 Size: 1440 610 3774 Fragments arranged by size: 3774 1440 610 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2493 2967 3263 513 Size: 552 1428 474 296 3074 Fragments arranged by size: 3074 1428 552 474 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3851 3868 4021 4206 4651 325 Size: 1255 2271 17 153 185 445 1498 Fragments arranged by size: 2271 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 2703 4070 Size: 274 80 33 51 1443 444 282 1367 Cuts at: 4070 5782 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1367 444 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2237 2269 2277 2309 5784 5816 2237 Size: 32 8 32 3475 32 2245 Fragments arranged by size: 3475 2245 32 32 32 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 2624 3953 2624 Size: 1329 4495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI