Restriction map for
pFN21ASDA1036
(Linear) (Six-base) MAPSORT of: KIAA1036.seq Check: 4921 from: 1 to: 1095
KIAA1036.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 343 1095
Size: 343 752
Acc65I G'GTAC_C
Cuts at: 0 1079 1095
Size: 1079 16
AfeI AGC'GCT
Cuts at: 0 379 562 1095
Size: 379 183 533
Fragments arranged by size:
533 379 183
AflIII A'CryG_T
Cuts at: 0 237 1095
Size: 237 858
ApaI G_GGCC'C
Cuts at: 0 931 984 1095
Size: 931 53 111
Fragments arranged by size:
931 111 53
ApoI r'AATT_y
Cuts at: 0 527 1095
Size: 527 568
AvaI C'yCGr_G
Cuts at: 0 1029 1095
Size: 1029 66
BamHI G'GATC_C
Cuts at: 0 281 1095
Size: 281 814
BanI G'GyrC_C
Cuts at: 0 40 1079 1095
Size: 40 1039 16
Fragments arranged by size:
1039 40 16
BanII G_rGCy'C
Cuts at: 0 716 931 984 1055 1095
Size: 716 215 53 71 40
Fragments arranged by size:
716 215 71 53 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 1048 1095
Size: 1048 47
BbvCI CC'TCA_GC
Cuts at: 0 111 1095
Size: 111 984
BlpI GC'TnA_GC
Cuts at: 0 706 1095
Size: 706 389
Bme1580I G_kGCm'C
Cuts at: 0 931 984 1095
Size: 931 53 111
Fragments arranged by size:
931 111 53
BmgBI CAC'GTC
Cuts at: 0 843 1095
Size: 843 252
BmrI ACTGGGnnnn_n'
Cuts at: 0 978 1095
Size: 978 117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 461 1095
Size: 461 634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 901 1095
Size: 577 324 194
Fragments arranged by size:
577 324 194
Bpu10I CC'TnA_GC
Cuts at: 0 111 356 1095
Size: 111 245 739
Fragments arranged by size:
739 245 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 892 1095
Size: 745 147 203
Fragments arranged by size:
745 203 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 1095
Size: 88 1007
BsaAI yAC'GTr
Cuts at: 0 240 756 1095
Size: 240 516 339
Fragments arranged by size:
516 339 240
BsaHI Gr'CG_yC
Cuts at: 0 170 1095
Size: 170 925
BsaWI w'CCGG_w
Cuts at: 0 950 1095
Size: 950 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 254 284 915 945 1095
Size: 254 30 631 30 150
Fragments arranged by size:
631 254 150 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 1095
Size: 142 953
BseYI C'CCAG_C
Cuts at: 0 22 618 651 774 1095
Size: 22 596 33 123 321
Fragments arranged by size:
596 321 123 33 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1095
Size: 394 701
BsiEI CG_ry'CG
Cuts at: 0 744 1095
Size: 744 351
BsiHKAI G_wGCw'C
Cuts at: 0 716 761 1095
Size: 716 45 334
Fragments arranged by size:
716 334 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 155 1095
Size: 155 940
Bsp1286I G_dGCh'C
Cuts at: 0 716 761 931 984 1055 1095
Size: 716 45 170 53 71 40
Fragments arranged by size:
716 170 71 53 45 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 308 1095
Size: 164 144 787
Fragments arranged by size:
787 164 144
BsrGI T'GTAC_A
Cuts at: 0 680 1095
Size: 680 415
BssSI C'ACGA_G
Cuts at: 0 715 1095
Size: 715 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 834 1095
Size: 834 261
BstXI CCAn_nnnn'nTGG
Cuts at: 0 259 1095
Size: 259 836
BstYI r'GATC_y
Cuts at: 0 248 281 308 1085 1095
Size: 248 33 27 777 10
Fragments arranged by size:
777 248 33 27 10
BtgI C'CryG_G
Cuts at: 0 285 1095
Size: 285 810
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 875 1095
Size: 875 220
BtsI GCAGTG_nn'
Cuts at: 0 1020 1095
Size: 1020 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 174 209 1095
Size: 174 35 886
Fragments arranged by size:
886 174 35
DraIII CAC_nnn'GTG
Cuts at: 0 615 807 834 1095
Size: 615 192 27 261
Fragments arranged by size:
615 261 192 27
DrdI GACnn_nn'nnGTC
Cuts at: 0 169 1095
Size: 169 926
EaeI y'GGCC_r
Cuts at: 0 62 242 467 741 1095
Size: 62 180 225 274 354
Fragments arranged by size:
354 274 225 180 62
EagI C'GGCC_G
Cuts at: 0 741 1095
Size: 741 354
EarI CTCTTCn'nnn_
Cuts at: 0 131 143 430 1095
Size: 131 12 287 665
Fragments arranged by size:
665 287 131 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 595 1095
Size: 595 500
EcoICRI GAG'CTC
Cuts at: 0 714 1095
Size: 714 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 901 1095
Size: 577 324 194
Fragments arranged by size:
577 324 194
EcoNI CCTnn'n_nnAGG
Cuts at: 0 222 449 469 1095
Size: 222 227 20 626
Fragments arranged by size:
626 227 222 20
EcoO109I rG'GnC_Cy
Cuts at: 0 153 489 672 928 1095
Size: 153 336 183 256 167
Fragments arranged by size:
336 256 183 167 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 603 1095
Size: 603 492
HaeII r_GCGC'y
Cuts at: 0 363 381 564 852 888 1095
Size: 363 18 183 288 36 207
Fragments arranged by size:
363 288 207 183 36 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 915 947 1095
Size: 915 32 148
Fragments arranged by size:
915 148 32
HincII GTy'rAC
Cuts at: 0 186 1095
Size: 186 909
KpnI G_GTAC'C
Cuts at: 0 1083 1095
Size: 1083 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1095
Size: 73 1022
MscI TGG'CCA
Cuts at: 0 244 469 1095
Size: 244 225 626
Fragments arranged by size:
626 244 225
MslI CAynn'nnrTG
Cuts at: 0 257 1095
Size: 257 838
MspA1I CmG'CkG
Cuts at: 0 38 58 746 978 1095
Size: 38 20 688 232 117
Fragments arranged by size:
688 232 117 38 20
NspI r_CATG'y
Cuts at: 0 549 903 1095
Size: 549 354 192
Fragments arranged by size:
549 354 192
PmlI CAC'GTG
Cuts at: 0 240 756 1095
Size: 240 516 339
Fragments arranged by size:
516 339 240
PpuMI rG'GwC_Cy
Cuts at: 0 153 672 1095
Size: 153 519 423
Fragments arranged by size:
519 423 153
PspOMI G'GGCC_C
Cuts at: 0 927 980 1095
Size: 927 53 115
Fragments arranged by size:
927 115 53
PstI C_TGCA'G
Cuts at: 0 398 1095
Size: 398 697
SacI G_AGCT'C
Cuts at: 0 716 1095
Size: 716 379
SapI GCTCTTCn'nnn_
Cuts at: 0 430 1095
Size: 430 665
SfcI C'TryA_G
Cuts at: 0 394 1095
Size: 394 701
SmlI C'TyrA_G
Cuts at: 0 760 907 1095
Size: 760 147 188
Fragments arranged by size:
760 188 147
SphI G_CATG'C
Cuts at: 0 549 1095
Size: 549 546
StyI C'CwwG_G
Cuts at: 0 470 944 1095
Size: 470 474 151
Fragments arranged by size:
474 470 151
TatI w'GTAC_w
Cuts at: 0 398 680 1095
Size: 398 282 415
Fragments arranged by size:
415 398 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 254 286 1095
Size: 214 32 8 32 809
Fragments arranged by size:
809 214 32 32 8
XmnI GAAnn'nnTTC
Cuts at: 0 601 1095
Size: 601 494
Enzymes that do cut:
AccI Acc65I AfeI AflIII ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NspI PmlI PpuMI
PspOMI PstI SacI SapI SfcI SmlI SphI StyI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglI BglII
BmtI BsaBI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BstBI BstEII BstZ17I Bsu36I ClaI DraI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI