Restriction map for
pFN21ASDA1034
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AvaI AviII AvrII BcgI BciVI
BclI BfrI BlnI BsaWI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI FbaI FseI FspI HincII
HindII HpaI MfeI MluI MroI
MslI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PpuMI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI SexAI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 64 GACGTC GACGT^C
Acc65I 2000 GGTACC G^GTACC
AflIII 1707 ACACGT A^C[GA][CT]GT
Asp718I 2000 GGTACC G^GTACC
AspI 2085 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1713 GGTCC G^G[AT]CC
BalI 152 TGGCCA TGG^CCA
BamHI 1880 GGATCC G^GATCC
BbvCI 2143 GCTGAGG CCTCAGC(-5/-2)
BfaI 1234 CTAG C^TAG
BglI 1516 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1859 AGATCT A^GATCT
BlpI 1948 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1948 GCTCAGC GC^T[ACGT]AGC
BsaBI 433 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1735 GTGCAG GTGCAG(16/14)
BsiEI 42 CGACCG CG[GA][CT]^CG
Bsp1407I 2129 TGTACA T^GTACA
BsrDI 527 GCAATG GCAATG(2/0)
BsrGI 2129 TGTACA T^GTACA
BstAPI 2173 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1948 GCTCAGC GC^T[ACGT]AGC
DraII 72 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 72 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1087 GATATC GAT^ATC
EcoT22I 757 ATGCAT ATGCA^T
HindIII 1890 AAGCTT A^AGCTT
KpnI 2000 GGTACC GGTAC^C
MaeI 1234 CTAG C^TAG
MamI 433 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 152 TGGCCA TGG^CCA
MscI 152 TGGCCA TGG^CCA
NaeI 971 GCCGGC GCC^GGC
NgoMIV 971 GCCGGC G^CCGGC
NsiI 757 ATGCAT ATGCA^T
PflFI 2085 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 282 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1751 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 835 AGTACT AGT^ACT
SphI 1700 GCATGC GCATG^C
SspBI 2129 TGTACA T^GTACA
StuI 1908 AGGCCT AGG^CCT
TaqI 1396 TCGA T^CGA
Tth111I 2085 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1396 TCGA T^CGA
Van91I 282 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 824 AAATTC [GA]^AATT[CT]
1257 GAATTT
AlwNI 1012 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2174 CAGCACCTG
AocI 1242 CCTGAGG CC^T[ACGT]AGG
1381 CCTCAGG
ApoI 824 AAATTC [GA]^AATT[CT]
1257 GAATTT
AspHI 564 GAGCAC G[AT]GC[AT]^C
2032 GAGCAC
BbeI 145 GGCGCC GGCGC^C
1432 GGCGCC
BbrPI 1708 CACGTG CAC^GTG
2005 CACGTG
BsaAI 1708 CACGTG [CT]AC^GT[GA]
2005 CACGTG
BsaI 1341 GGTCTC GGTCTC(1/5)
1847 GGTCTC
BsiHKAI 564 GAGCAC G[AT]GC[AT]^C
2032 GAGCAC
BsmI 580 GAATGC GAATGC(1/-1)
1156 GCATTC
BspHI 875 TCATGA T^CATGA
1899 TCATGA
BspMI 2178 ACCTGC ACCTGC(4/8)
1737 GCAGGT
BsrI 82 CCAGT ACTGG(1/-1)
1124 CCAGT
BstXI 155 CCAAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
657 CCAGGAGTTTGG
Bsu36I 1242 CCTGAGG CC^T[ACGT]AGG
1381 CCTCAGG
EaeI 152 TGGCCA [CT]^GGCC[GA]
416 TGGCCG
Eco81I 1242 CCTGAGG CC^T[ACGT]AGG
1381 CCTCAGG
HgaI 406 GACGC GACGC(5/10)
750 GCGTC
KasI 145 GGCGCC G^GCGCC
1432 GGCGCC
KspI 129 CCGCGG CCGC^GG
2045 CCGCGG
MaeIII 391 GTGAC ^GT[ACGT]AC
448 GTGAC
NarI 145 GGCGCC GG^CGCC
1432 GGCGCC
NcoI 119 CCATGG C^CATGG
935 CCATGG
NspI 422 ACATGC [GA]CATG^[CT]
1700 GCATGC
PleI 1675 GAGTC GAGTC(4/5)
2089 GAGTC
PmaCI 1708 CACGTG CAC^GTG
2005 CACGTG
PmlI 1708 CACGTG CAC^GTG
2005 CACGTG
RcaI 875 TCATGA T^CATGA
1899 TCATGA
SacII 129 CCGCGG CCGC^GG
2045 CCGCGG
SfoI 145 GGCGCC GGC^GCC
1432 GGCGCC
SmlI 995 CTCAAG C^T[CT][GA]AG
1502 CTCAAG
Tsp45I 391 GTGAC ^GT[GC]AC
448 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI BanI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrFI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp509I TspRI
XcmI XhoII