(Circular) (Six-base) MAPSORT of: pFN21ASDA1034.seq Check: 6912 from: 1 to: 6928 pFN21ASDA1034 6928 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4210 4210 Size: 6928 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2091 4738 278 Size: 53 83 186 936 555 2647 2468 Fragments arranged by size: 2647 2468 936 555 186 83 53 AccI GT'mk_AC Cuts at: 1937 4271 1937 Size: 2334 4594 Acc65I G'GTAC_C Cuts at: 4023 4249 4023 Size: 226 6702 AclI AA'CG_TT Cuts at: 1563 5055 5428 1563 Size: 3492 373 3063 Fragments arranged by size: 3492 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3158 3864 4991 6375 6643 1632 Size: 1526 706 1127 1384 268 1917 Fragments arranged by size: 1917 1526 1384 1127 706 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6731 AflIII A'CryG_T Cuts at: 1203 3730 5827 6804 1203 Size: 2527 2097 977 1327 Fragments arranged by size: 2527 2097 1327 977 AgeI A'CCGG_T Cuts at: 5759 5759 Size: 6928 AhdI GACnn_n'nnGTC Cuts at: 5657 5657 Size: 6928 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6928 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2692 1184 Size: 1508 5420 AlwNI CAG_nnn'CTG Cuts at: 3040 4202 4671 6243 3040 Size: 1162 469 1572 3725 Fragments arranged by size: 3725 1572 1162 469 ApaLI G'TGCA_C Cuts at: 4985 6141 4985 Size: 1156 5772 ApoI r'AATT_y Cuts at: 1493 2847 3280 4237 4463 4615 6724 1493 Size: 1354 433 957 226 152 2109 1697 Fragments arranged by size: 2109 1697 1354 957 433 226 152 AseI AT'TA_AT Cuts at: 160 1032 5482 160 Size: 872 4450 1606 Fragments arranged by size: 4450 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 AvaI C'yCGr_G Cuts at: 1870 1943 4253 1870 Size: 73 2310 4545 Fragments arranged by size: 4545 2310 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3622 3655 3622 Size: 33 6895 BamHI G'GATC_C Cuts at: 3903 4258 4625 3903 Size: 355 367 6206 Fragments arranged by size: 6206 367 355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 3455 4023 4249 5704 Size: 325 217 627 381 1287 568 226 1455 Cuts at: 5704 618 Size: 1842 Fragments arranged by size: 1842 1455 1287 627 568 381 325 226 217 BanII G_rGCy'C Cuts at: 1391 2045 2152 2906 4067 4247 1391 Size: 654 107 754 1161 180 4072 Fragments arranged by size: 4072 1161 754 654 180 107 BbeI G_GCGC'C Cuts at: 2172 3459 2172 Size: 1287 5641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2517 2903 3234 3852 928 Size: 971 618 386 331 618 4004 Fragments arranged by size: 4004 971 618 618 386 331 BbvCI CC'TCA_GC Cuts at: 4167 4167 Size: 6928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5119 5153 1903 Size: 34 3182 34 3678 Fragments arranged by size: 3678 3182 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4820 6030 4820 Size: 1210 5718 BclI T'GATC_A Cuts at: 1548 1548 Size: 6928 BfrBI ATG'CAT Cuts at: 2782 2782 Size: 6928 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3545 5539 136 Size: 107 122 71 631 605 1873 1994 1525 Fragments arranged by size: 1994 1873 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3882 6923 3882 Size: 3041 3887 BlpI GC'TnA_GC Cuts at: 3972 4345 3972 Size: 373 6555 Bme1580I G_kGCm'C Cuts at: 1790 2883 4989 6145 1790 Size: 1093 2106 1156 2573 Fragments arranged by size: 2573 2106 1156 1093 BmgBI CAC'GTC Cuts at: 1318 4078 1318 Size: 2760 4168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2099 4659 5617 Size: 416 53 83 399 24 682 2560 958 Cuts at: 5617 442 Size: 1753 Fragments arranged by size: 2560 1753 958 682 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6928 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3822 4156 3822 Size: 334 6594 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 3090 3361 5588 1239 Size: 905 946 271 2227 2579 Fragments arranged by size: 2579 2227 946 905 271 Bpu10I CC'TnA_GC Cuts at: 3750 4167 3750 Size: 417 6511 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3003 3510 5053 5918 6216 3003 Size: 507 1543 865 298 3715 Fragments arranged by size: 3715 1543 865 507 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3370 3876 5591 882 Size: 617 1871 506 1715 2219 Fragments arranged by size: 2219 1871 1715 617 506 BsaAI yAC'GTr Cuts at: 493 1204 3733 4030 4225 4719 493 Size: 711 2529 297 195 494 2702 Fragments arranged by size: 2702 2529 711 494 297 195 BsaBI GATnn'nnATC Cuts at: 2460 6922 2460 Size: 4462 2466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2169 3456 4735 Size: 53 83 186 936 555 81 1287 1279 Cuts at: 4735 5117 5752 275 Size: 382 635 1451 Fragments arranged by size: 1451 1287 1279 936 635 555 382 186 83 81 53 BsaWI w'CCGG_w Cuts at: 5361 5759 6033 6180 6591 5361 Size: 398 274 147 411 5698 Fragments arranged by size: 5698 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4049 4079 1178 Size: 30 165 30 2646 30 4027 Fragments arranged by size: 4027 2646 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2826 3358 3382 3707 3710 3825 4112 4133 Size: 1640 532 24 325 3 115 287 21 Cuts at: 4133 4160 4184 1186 Size: 27 24 3930 Fragments arranged by size: 3930 1640 532 325 287 115 27 24 24 21 3 BseYI C'CCAG_C Cuts at: 2380 2922 3386 3666 6131 6627 2380 Size: 542 464 280 2465 496 2681 Fragments arranged by size: 2681 2465 542 496 464 280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3779 1163 Size: 573 2043 4312 Fragments arranged by size: 4312 2043 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2068 4378 5139 6167 1540 Size: 275 186 67 2310 761 1028 2301 Fragments arranged by size: 2310 2301 1028 761 275 186 67 BsiHKAI G_wGCw'C Cuts at: 2591 4059 4989 5074 6145 2591 Size: 1468 930 85 1071 3374 Fragments arranged by size: 3374 1468 1071 930 85 BsmI GAATG_Cn' Cuts at: 2609 3179 4439 4532 2609 Size: 570 1260 93 5005 Fragments arranged by size: 5005 1260 570 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2152 2591 2883 2906 4059 4067 Size: 399 255 107 439 292 23 1153 8 Cuts at: 4067 4247 4989 5074 6145 1391 Size: 180 742 85 1071 2174 Fragments arranged by size: 2174 1153 1071 742 439 399 292 255 180 107 85 23 8 BspEI T'CCGG_A Cuts at: 6591 6591 Size: 6928 BspHI T'CATG_A Cuts at: 2898 3922 4817 6547 2898 Size: 1024 895 1730 3279 Fragments arranged by size: 3279 1730 1024 895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3751 4210 4283 844 Size: 2907 459 73 3489 Fragments arranged by size: 3489 2907 459 73 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2076 3337 4815 1394 Size: 429 206 47 1261 1478 3507 Fragments arranged by size: 3507 1478 1261 429 206 47 BsrDI GCAATG_nn' Cuts at: 65 1248 2557 5423 5597 65 Size: 1183 1309 2866 174 1396 Fragments arranged by size: 2866 1396 1309 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 2994 3754 5572 5759 1700 Size: 110 257 927 760 1818 187 2869 Fragments arranged by size: 2869 1818 927 760 257 187 110 BsrGI T'GTAC_A Cuts at: 96 4152 96 Size: 4056 2872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6928 BssSI C'ACGA_G Cuts at: 4988 6000 4988 Size: 1012 5916 BstAPI GCAn_nnn'nTGC Cuts at: 794 4202 794 Size: 3408 3520 BstBI TT'CG_AA Cuts at: 2007 5732 2007 Size: 3725 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6928 BstXI CCAn_nnnn'nTGG Cuts at: 2185 2687 2185 Size: 502 6426 BstYI r'GATC_y Cuts at: 1972 3492 3882 3903 3979 4258 4625 5010 5027 Size: 1520 390 21 76 279 367 385 17 Cuts at: 5027 6479 6565 6577 6923 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1520 1452 390 385 367 346 279 86 76 21 17 12 Bsu36I CC'TnA_GG Cuts at: 3266 3405 3266 Size: 139 6789 BtgI C'CryG_G Cuts at: 513 1065 2022 2142 2152 2172 2184 2958 3093 Size: 552 957 120 10 20 12 774 135 Cuts at: 3093 4039 4068 513 Size: 946 29 3373 Fragments arranged by size: 3373 957 946 774 552 135 120 29 20 12 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6189 Fragments arranged by size: 6189 683 56 BtsI GCAGTG_nn' Cuts at: 2534 4444 5238 5258 2534 Size: 1910 794 20 4204 Fragments arranged by size: 4204 1910 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6928 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6893 DraI TTT'AAA Cuts at: 4232 4232 Size: 6928 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5935 809 Size: 507 4619 1802 Fragments arranged by size: 4619 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2439 4375 Size: 54 1425 50 275 18 345 264 1936 Cuts at: 4375 5264 6893 8 Size: 889 1629 43 Fragments arranged by size: 1936 1629 1425 889 345 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4375 1537 Size: 275 2563 4090 Fragments arranged by size: 4090 2563 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3094 3134 3258 4858 1353 Size: 1741 40 124 1600 3423 Fragments arranged by size: 3423 1741 1600 124 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2047 2086 5515 5889 6035 387 Size: 1064 138 458 39 3429 374 146 1280 Fragments arranged by size: 3429 1280 1064 458 374 146 138 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 3090 3158 3361 3864 4991 5588 Size: 393 512 946 68 203 503 1127 597 Cuts at: 5588 6375 6643 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1127 946 787 597 512 503 393 268 203 68 EcoO109I rG'GnC_Cy Cuts at: 2096 2096 Size: 6928 EcoRI G'AATT_C Cuts at: 4237 4615 4237 Size: 378 6550 EcoRV GAT'ATC Cuts at: 3112 3112 Size: 6928 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2312 3904 2312 Size: 1592 5336 FspI TGC'GCA Cuts at: 5434 5434 Size: 6928 HaeII r_GCGC'y Cuts at: 1678 2172 3459 3824 6075 1678 Size: 494 1287 365 2251 2531 Fragments arranged by size: 2531 2251 1287 494 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2032 2064 2893 2925 3229 Size: 32 135 32 460 32 829 32 304 Cuts at: 3229 3261 3300 3331 3332 3363 3683 3715 5569 Size: 32 39 31 1 31 320 32 1854 Cuts at: 5569 5601 5643 5675 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1854 829 460 320 304 135 42 39 32 32 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 4272 4518 669 Size: 988 281 2334 246 3079 Fragments arranged by size: 3079 2334 988 281 246 HindIII A'AGCT_T Cuts at: 748 3913 748 Size: 3165 3763 HpaI GTT'AAC Cuts at: 4518 4518 Size: 6928 KasI G'GCGC_C Cuts at: 2168 3455 2168 Size: 1287 5641 KpnI G_GTAC'C Cuts at: 4027 4253 4027 Size: 226 6702 MfeI C'AATT_G Cuts at: 4527 4527 Size: 6928 MluI A'CGCG_T Cuts at: 6804 6804 Size: 6928 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 2487 3520 3863 6042 6226 1305 Size: 903 279 1033 343 2179 184 2007 Fragments arranged by size: 2179 2007 1033 903 343 279 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 10 Size: 54 1425 343 345 4761 Fragments arranged by size: 4761 1425 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4886 5245 5404 518 Size: 648 3720 359 159 2042 Fragments arranged by size: 3720 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2154 2308 2803 3476 3588 3976 4021 4070 Size: 548 154 495 673 112 388 45 49 Cuts at: 4070 4344 5021 6169 6414 1606 Size: 274 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 673 548 495 388 274 245 154 112 49 45 NaeI GCC'GGC Cuts at: 2996 2996 Size: 6928 NarI GG'CG_CC Cuts at: 2169 3456 2169 Size: 1287 5641 NcoI C'CATG_G Cuts at: 513 1065 2022 2142 2958 513 Size: 552 957 120 816 4483 Fragments arranged by size: 4483 957 816 552 120 NdeI CA'TA_TG Cuts at: 387 387 Size: 6928 NgoMIV G'CCGG_C Cuts at: 2994 2994 Size: 6928 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6928 NotI GC'GGCC_GC Cuts at: 4375 4375 Size: 6928 NsiI A_TGCA'T Cuts at: 2784 2784 Size: 6928 NspI r_CATG'y Cuts at: 1132 1229 2449 3727 4286 5831 1132 Size: 97 1220 1278 559 1545 2229 Fragments arranged by size: 2229 1545 1278 1220 559 97 PasI CC'CwG_GG Cuts at: 1348 2332 2812 3898 1348 Size: 984 480 1086 4378 Fragments arranged by size: 4378 1086 984 480 PciI A'CATG_T Cuts at: 5827 5827 Size: 6928 PflMI CCAn_nnn'nTGG Cuts at: 1108 2311 1108 Size: 1203 5725 PmeI GTTT'AAAC Cuts at: 4232 4232 Size: 6928 PmlI CAC'GTG Cuts at: 3733 4030 3733 Size: 297 6631 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4997 5029 6510 6542 4997 Size: 32 1481 32 5383 Fragments arranged by size: 5383 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3778 1613 Size: 2165 4763 PsiI TTA'TAA Cuts at: 4498 4498 Size: 6928 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4007 4039 4007 Size: 32 6896 PstI C_TGCA'G Cuts at: 830 4280 830 Size: 3450 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6928 PvuII CAG'CTG Cuts at: 2308 2803 3476 3976 2308 Size: 495 673 500 5260 Fragments arranged by size: 5260 673 500 495 SacII CC_GC'GG Cuts at: 2155 4071 2155 Size: 1916 5012 SalI G'TCGA_C Cuts at: 1936 4270 1936 Size: 2334 4594 SbfI CC_TGCA'GG Cuts at: 4280 4280 Size: 6928 ScaI AGT'ACT Cuts at: 2860 5176 6888 2860 Size: 2316 1712 2900 Fragments arranged by size: 2900 2316 1712 SfcI C'TryA_G Cuts at: 826 1045 4276 5411 6092 6283 826 Size: 219 3231 1135 681 191 1471 Fragments arranged by size: 3231 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2170 3457 2170 Size: 1287 5641 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6928 SmaI CCC'GGG Cuts at: 1872 4255 1872 Size: 2383 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3018 3525 5032 5933 6195 820 Size: 197 926 1075 507 1507 901 262 1553 Fragments arranged by size: 1553 1507 1075 926 901 507 262 197 SnaBI TAC'GTA Cuts at: 493 4225 4719 493 Size: 3732 494 2702 Fragments arranged by size: 3732 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6928 SphI G_CATG'C Cuts at: 1132 3727 4286 1132 Size: 2595 559 3774 Fragments arranged by size: 3774 2595 559 SspI AAT'ATT Cuts at: 5 52 4852 5 Size: 47 4800 2081 Fragments arranged by size: 4800 2081 47 StuI AGG'CCT Cuts at: 1517 3933 1517 Size: 2416 4512 StyI C'CwwG_G Cuts at: 513 1065 2022 2142 2178 2365 2958 3516 3792 Size: 552 957 120 36 187 593 558 276 Cuts at: 3792 4367 513 Size: 575 3074 Fragments arranged by size: 3074 957 593 575 558 552 276 187 120 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3067 4124 4955 4972 5125 5310 5755 Size: 1255 1487 1057 831 17 153 185 445 Cuts at: 5755 325 Size: 1498 Fragments arranged by size: 1498 1487 1255 1057 831 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 2858 4152 Size: 274 80 33 51 1443 729 152 1294 Cuts at: 4152 5174 6886 96 Size: 1022 1712 138 Fragments arranged by size: 1712 1443 1294 1022 729 274 152 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4049 4081 6888 6920 4049 Size: 32 2807 32 4057 Fragments arranged by size: 4057 2807 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4111 1316 Size: 2795 4133 XbaI T'CTAG_A Cuts at: 4264 4264 Size: 6928 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2170 3091 3358 2170 Size: 921 267 5740 Fragments arranged by size: 5740 921 267 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6928 XmaI C'CCGG_G Cuts at: 1870 4253 1870 Size: 2383 4545 XmnI GAAnn'nnTTC Cuts at: 5057 5057 Size: 6928 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2089 4736 276 Size: 53 83 186 936 555 2647 2468 Fragments arranged by size: 2647 2468 936 555 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NruI PacI PfoI PpuMI PspOMI PspXI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI