Restriction map for
pFN21ASDA1034
(Linear) (Six-base) MAPSORT of: KIAA1034.seq Check: 1740 from: 1 to: 2199
KIAA1034.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2187 2199
Size: 2187 12
AatII G_ACGT'C
Cuts at: 0 68 2199
Size: 68 2131
Acc65I G'GTAC_C
Cuts at: 0 2000 2199
Size: 2000 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1135 1841 2199
Size: 1135 706 358
Fragments arranged by size:
1135 706 358
AflIII A'CryG_T
Cuts at: 0 1707 2199
Size: 1707 492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 669 2199
Size: 669 1530
AlwNI CAG_nnn'CTG
Cuts at: 0 1017 2179 2199
Size: 1017 1162 20
Fragments arranged by size:
1162 1017 20
ApoI r'AATT_y
Cuts at: 0 824 1257 2199
Size: 824 433 942
Fragments arranged by size:
942 824 433
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1599 1632 2199
Size: 1599 33 567
Fragments arranged by size:
1599 567 33
BamHI G'GATC_C
Cuts at: 0 1880 2199
Size: 1880 319
BanI G'GyrC_C
Cuts at: 0 145 1432 2000 2199
Size: 145 1287 568 199
Fragments arranged by size:
1287 568 199 145
BanII G_rGCy'C
Cuts at: 0 22 129 883 2044 2199
Size: 22 107 754 1161 155
Fragments arranged by size:
1161 754 155 107 22
BbeI G_GCGC'C
Cuts at: 0 149 1436 2199
Size: 149 1287 763
Fragments arranged by size:
1287 763 149
BbsI GAAGACnn'nnnn_
Cuts at: 0 494 880 1211 1829 2199
Size: 494 386 331 618 370
Fragments arranged by size:
618 494 386 370 331
BbvCI CC'TCA_GC
Cuts at: 0 2144 2199
Size: 2144 55
BfrBI ATG'CAT
Cuts at: 0 759 2199
Size: 759 1440
BglI GCCn_nnn'nGGC
Cuts at: 0 1522 2199
Size: 1522 677
BglII A'GATC_T
Cuts at: 0 1859 2199
Size: 1859 340
BlpI GC'TnA_GC
Cuts at: 0 1949 2199
Size: 1949 250
Bme1580I G_kGCm'C
Cuts at: 0 860 2199
Size: 860 1339
BmgBI CAC'GTC
Cuts at: 0 2055 2199
Size: 2055 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 76 2199
Size: 76 2123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1799 2133 2199
Size: 1799 334 66
Fragments arranged by size:
1799 334 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1067 1338 2199
Size: 121 946 271 861
Fragments arranged by size:
946 861 271 121
Bpu10I CC'TnA_GC
Cuts at: 0 1727 2144 2199
Size: 1727 417 55
Fragments arranged by size:
1727 417 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1487 2199
Size: 980 507 712
Fragments arranged by size:
980 712 507
BsaI GGTCTCn'nnnn_
Cuts at: 0 1347 1853 2199
Size: 1347 506 346
Fragments arranged by size:
1347 506 346
BsaAI yAC'GTr
Cuts at: 0 1710 2007 2199
Size: 1710 297 192
Fragments arranged by size:
1710 297 192
BsaBI GATnn'nnATC
Cuts at: 0 437 2199
Size: 437 1762
BsaHI Gr'CG_yC
Cuts at: 0 65 146 1433 2199
Size: 65 81 1287 766
Fragments arranged by size:
1287 766 81 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2026 2056 2199
Size: 2026 30 143
Fragments arranged by size:
2026 143 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 803 1335 1359 1684 1687 1802 2089 2110
Size: 803 532 24 325 3 115 287 21
Cuts at: 2110 2137 2161 2199
Size: 27 24 38
Fragments arranged by size:
803 532 325 287 115 38 27 24
24 21 3
BseYI C'CCAG_C
Cuts at: 0 357 899 1363 1643 2199
Size: 357 542 464 280 556
Fragments arranged by size:
556 542 464 357 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1756 2199
Size: 1756 443
BsiEI CG_ry'CG
Cuts at: 0 45 2199
Size: 45 2154
BsiHKAI G_wGCw'C
Cuts at: 0 568 2036 2199
Size: 568 1468 163
Fragments arranged by size:
1468 568 163
BsmI GAATG_Cn'
Cuts at: 0 586 1156 2199
Size: 586 570 1043
Fragments arranged by size:
1043 586 570
Bsp1286I G_dGCh'C
Cuts at: 0 22 129 568 860 883 2036 2044 2199
Size: 22 107 439 292 23 1153 8 155
Fragments arranged by size:
1153 439 292 155 107 23 22 8
BspHI T'CATG_A
Cuts at: 0 875 1899 2199
Size: 875 1024 300
Fragments arranged by size:
1024 875 300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1728 2187 2199
Size: 1728 459 12
Fragments arranged by size:
1728 459 12
BsrBI CCG'CTC
Cuts at: 0 6 53 1314 2199
Size: 6 47 1261 885
Fragments arranged by size:
1261 885 47 6
BsrDI GCAATG_nn'
Cuts at: 0 534 2199
Size: 534 1665
BsrFI r'CCGG_y
Cuts at: 0 44 971 1731 2199
Size: 44 927 760 468
Fragments arranged by size:
927 760 468 44
BsrGI T'GTAC_A
Cuts at: 0 2129 2199
Size: 2129 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2179 2199
Size: 2179 20
BstXI CCAn_nnnn'nTGG
Cuts at: 0 162 664 2199
Size: 162 502 1535
Fragments arranged by size:
1535 502 162
BstYI r'GATC_y
Cuts at: 0 1469 1859 1880 1956 2199
Size: 1469 390 21 76 243
Fragments arranged by size:
1469 390 243 76 21
Bsu36I CC'TnA_GG
Cuts at: 0 1243 1382 2199
Size: 1243 139 817
Fragments arranged by size:
1243 817 139
BtgI C'CryG_G
Cuts at: 0 119 129 149 161 935 1070 2016 2045
Size: 119 10 20 12 774 135 946 29
Cuts at: 2045 2199
Size: 154
Fragments arranged by size:
946 774 154 135 119 29 20 12
10
BtsI GCAGTG_nn'
Cuts at: 0 511 2199
Size: 511 1688
EaeI y'GGCC_r
Cuts at: 0 152 416 2199
Size: 152 264 1783
Fragments arranged by size:
1783 264 152
EarI CTCTTCn'nnn_
Cuts at: 0 1071 1111 1235 2199
Size: 1071 40 124 964
Fragments arranged by size:
1071 964 124 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 63 2199
Size: 24 39 2136
Fragments arranged by size:
2136 39 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1067 1135 1338 1841 2199
Size: 121 946 68 203 503 358
Fragments arranged by size:
946 503 358 203 121 68
EcoO109I rG'GnC_Cy
Cuts at: 0 73 2199
Size: 73 2126
EcoRV GAT'ATC
Cuts at: 0 1089 2199
Size: 1089 1110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 289 1881 2199
Size: 289 1592 318
Fragments arranged by size:
1592 318 289
HaeII r_GCGC'y
Cuts at: 0 149 1436 1801 2199
Size: 149 1287 365 398
Fragments arranged by size:
1287 398 365 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 9 41 870 902 1206 1238 1277 1308
Size: 9 32 829 32 304 32 39 31
Cuts at: 1308 1309 1340 1660 1692 2199
Size: 1 31 320 32 507
Fragments arranged by size:
829 507 320 304 39 32 32 32
32 31 31 9 1
HindIII A'AGCT_T
Cuts at: 0 1890 2199
Size: 1890 309
KasI G'GCGC_C
Cuts at: 0 145 1432 2199
Size: 145 1287 767
Fragments arranged by size:
1287 767 145
KpnI G_GTAC'C
Cuts at: 0 2004 2199
Size: 2004 195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 464 1497 1840 2199
Size: 185 279 1033 343 359
Fragments arranged by size:
1033 359 343 279 185
MscI TGG'CCA
Cuts at: 0 154 2199
Size: 154 2045
MspA1I CmG'CkG
Cuts at: 0 131 285 780 1453 1565 1953 1998 2047
Size: 131 154 495 673 112 388 45 49
Cuts at: 2047 2199
Size: 152
Fragments arranged by size:
673 495 388 154 152 131 112 49
45
NaeI GCC'GGC
Cuts at: 0 973 2199
Size: 973 1226
NarI GG'CG_CC
Cuts at: 0 146 1433 2199
Size: 146 1287 766
Fragments arranged by size:
1287 766 146
NcoI C'CATG_G
Cuts at: 0 119 935 2199
Size: 119 816 1264
Fragments arranged by size:
1264 816 119
NgoMIV G'CCGG_C
Cuts at: 0 971 2199
Size: 971 1228
NsiI A_TGCA'T
Cuts at: 0 761 2199
Size: 761 1438
NspI r_CATG'y
Cuts at: 0 426 1704 2199
Size: 426 1278 495
Fragments arranged by size:
1278 495 426
PasI CC'CwG_GG
Cuts at: 0 309 789 1875 2199
Size: 309 480 1086 324
Fragments arranged by size:
1086 480 324 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 288 2199
Size: 288 1911
PmlI CAC'GTG
Cuts at: 0 1710 2007 2199
Size: 1710 297 192
Fragments arranged by size:
1710 297 192
PshAI GACnn'nnGTC
Cuts at: 0 1755 2199
Size: 1755 444
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1984 2016 2199
Size: 1984 32 183
Fragments arranged by size:
1984 183 32
PvuII CAG'CTG
Cuts at: 0 285 780 1453 1953 2199
Size: 285 495 673 500 246
Fragments arranged by size:
673 500 495 285 246
SacII CC_GC'GG
Cuts at: 0 132 2048 2199
Size: 132 1916 151
Fragments arranged by size:
1916 151 132
ScaI AGT'ACT
Cuts at: 0 837 2199
Size: 837 1362
SfoI GGC'GCC
Cuts at: 0 147 1434 2199
Size: 147 1287 765
Fragments arranged by size:
1287 765 147
SmlI C'TyrA_G
Cuts at: 0 995 1502 2199
Size: 995 507 697
Fragments arranged by size:
995 697 507
SphI G_CATG'C
Cuts at: 0 1704 2199
Size: 1704 495
StuI AGG'CCT
Cuts at: 0 1910 2199
Size: 1910 289
StyI C'CwwG_G
Cuts at: 0 119 155 342 935 1493 1769 2199
Size: 119 36 187 593 558 276 430
Fragments arranged by size:
593 558 430 276 187 119 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1044 2101 2199
Size: 1044 1057 98
Fragments arranged by size:
1057 1044 98
TatI w'GTAC_w
Cuts at: 0 683 835 2129 2199
Size: 683 152 1294 70
Fragments arranged by size:
1294 683 152 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2026 2058 2199
Size: 2026 32 141
Fragments arranged by size:
2026 141 32
Tth111I GACn'n_nGTC
Cuts at: 0 2088 2199
Size: 2088 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 1068 1335 2199
Size: 147 921 267 864
Fragments arranged by size:
921 864 267 147
ZraI GAC'GTC
Cuts at: 0 66 2199
Size: 66 2133
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlfI AlwNI ApoI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I HindIII KasI KpnI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PflMI PmlI PshAI PsrI PvuII SacII ScaI SfoI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BcgI
BciVI BclI BmtI BsaWI BsiWI BsmBI BspEI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoRI FseI FspI
FspAI HincII HpaI MfeI MluI MslI NdeI NheI
NotI NruI PacI PciI PfoI PmeI PpiI PpuMI
PsiI PspOMI PspXI PstI PvuI RsrII SacI SalI
SanDI SapI SbfI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
XmaI XmnI