(Circular) (Six-base) MAPSORT of: pFN21ASDA1024.seq Check: 7542 from: 1 to: 7477 pFN21ASDA1024 7477 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4384 4384 Size: 7477 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5287 278 Size: 53 83 186 936 3751 2468 Fragments arranged by size: 3751 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4820 1937 Size: 2883 4594 Acc65I G'GTAC_C Cuts at: 4798 4798 Size: 7477 AclI AA'CG_TT Cuts at: 1563 5604 5977 1563 Size: 4041 373 3063 Fragments arranged by size: 4041 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3818 5540 6924 7192 1632 Size: 2186 1722 1384 268 1917 Fragments arranged by size: 2186 1917 1722 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7280 AflIII A'CryG_T Cuts at: 1203 6376 7353 1203 Size: 5173 977 1327 Fragments arranged by size: 5173 1327 977 AgeI A'CCGG_T Cuts at: 6308 6308 Size: 7477 AhdI GACnn_n'nnGTC Cuts at: 6206 6206 Size: 7477 AleI CACnn'nnGTG Cuts at: 1166 3053 3626 1166 Size: 1887 573 5017 Fragments arranged by size: 5017 1887 573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3836 1184 Size: 2652 4825 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4382 4414 4382 Size: 32 7445 AlwNI CAG_nnn'CTG Cuts at: 2110 2614 2959 3081 4218 5220 6792 2110 Size: 504 345 122 1137 1002 1572 2795 Fragments arranged by size: 2795 1572 1137 1002 504 345 122 ApaI G_GGCC'C Cuts at: 3048 3048 Size: 7477 ApaLI G'TGCA_C Cuts at: 3493 5534 6690 3493 Size: 2041 1156 4280 Fragments arranged by size: 4280 2041 1156 ApoI r'AATT_y Cuts at: 1493 4670 4786 5012 5164 7273 1493 Size: 3177 116 226 152 2109 1697 Fragments arranged by size: 3177 2109 1697 226 152 116 AseI AT'TA_AT Cuts at: 160 1032 6031 160 Size: 872 4999 1606 Fragments arranged by size: 4999 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7477 AvaI C'yCGr_G Cuts at: 1870 1943 4802 1870 Size: 73 2859 4545 Fragments arranged by size: 4545 2859 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3623 3656 3623 Size: 33 7444 BamHI G'GATC_C Cuts at: 3659 4807 5174 3659 Size: 1148 367 5962 Fragments arranged by size: 5962 1148 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3252 3446 4798 6253 618 Size: 325 217 627 1465 194 1352 1455 1842 Fragments arranged by size: 1842 1465 1455 1352 627 325 217 194 BanII G_rGCy'C Cuts at: 1391 2430 3048 3327 3474 4796 1391 Size: 1039 618 279 147 1322 4072 Fragments arranged by size: 4072 1322 1039 618 279 147 BbeI G_GCGC'C Cuts at: 3256 3256 Size: 7477 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3806 4626 928 Size: 971 1907 820 3779 Fragments arranged by size: 3779 1907 971 820 BbvCI CC'TCA_GC Cuts at: 2640 2640 Size: 7477 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5668 5702 1903 Size: 34 3731 34 3678 Fragments arranged by size: 3731 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4631 5369 6579 4631 Size: 738 1210 5529 Fragments arranged by size: 5529 1210 738 BclI T'GATC_A Cuts at: 1548 1548 Size: 7477 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6088 136 Size: 107 122 71 631 605 4416 1525 Fragments arranged by size: 4416 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2061 2403 3339 3955 7472 2061 Size: 342 936 616 3517 2066 Fragments arranged by size: 3517 2066 936 616 342 BlpI GC'TnA_GC Cuts at: 3422 4894 3422 Size: 1472 6005 Bme1580I G_kGCm'C Cuts at: 1790 2131 3048 3449 3497 4752 5538 6694 1790 Size: 341 917 401 48 1255 786 1156 2573 Fragments arranged by size: 2573 1255 1156 917 786 401 341 48 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7477 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2832 5208 6166 Size: 416 53 83 399 24 1415 2376 958 Cuts at: 6166 442 Size: 1753 Fragments arranged by size: 2376 1753 1415 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4083 1056 Size: 3027 4450 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3148 3380 3488 4617 6137 1239 Size: 1909 232 108 1129 1520 2579 Fragments arranged by size: 2579 1909 1520 1129 232 108 Bpu10I CC'TnA_GC Cuts at: 2640 3702 3824 2640 Size: 1062 122 6293 Fragments arranged by size: 6293 1062 122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4412 5602 6467 6765 4412 Size: 1190 865 298 5124 Fragments arranged by size: 5124 1190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2021 2732 3480 6140 882 Size: 617 522 711 748 2660 2219 Fragments arranged by size: 2660 2219 748 711 617 522 BsaAI yAC'GTr Cuts at: 493 1204 3463 4774 5268 493 Size: 711 2259 1311 494 2702 Fragments arranged by size: 2702 2259 1311 711 494 BsaBI GATnn'nnATC Cuts at: 7471 7471 Size: 7477 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3253 5284 5666 6301 Size: 53 83 186 936 1720 2031 382 635 Cuts at: 6301 275 Size: 1451 Fragments arranged by size: 2031 1720 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5910 6308 6582 6729 7140 5910 Size: 398 274 147 411 6247 Fragments arranged by size: 6247 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2693 2723 4013 4043 4078 Size: 30 165 30 1290 30 1290 30 35 Cuts at: 4078 4108 4382 4406 4412 4436 1178 Size: 30 274 24 6 24 4219 Fragments arranged by size: 4219 1290 1290 274 165 35 30 30 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2771 3206 4178 4274 4277 4535 1186 Size: 1585 435 972 96 3 258 4128 Fragments arranged by size: 4128 1585 972 435 258 96 3 BseYI C'CCAG_C Cuts at: 3238 6680 7176 3238 Size: 3442 496 3539 Fragments arranged by size: 3539 3442 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3391 3968 4741 1163 Size: 573 1655 577 773 3899 Fragments arranged by size: 3899 1655 773 577 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4927 5688 6716 1540 Size: 275 186 2926 761 1028 2301 Fragments arranged by size: 2926 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2877 3462 3497 3708 3825 5538 5623 6694 Size: 705 585 35 211 117 1713 85 1071 Cuts at: 6694 2172 Size: 2955 Fragments arranged by size: 2955 1713 1071 705 585 211 117 85 35 BsmI GAATG_Cn' Cuts at: 4540 4988 5081 4540 Size: 448 93 6936 Fragments arranged by size: 6936 448 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2172 2430 2877 3048 3327 3449 Size: 399 341 41 258 447 171 279 122 Cuts at: 3449 3462 3474 3497 3708 3825 4752 4796 5538 Size: 13 12 23 211 117 927 44 742 Cuts at: 5538 5623 6694 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 927 742 447 399 341 279 258 211 171 122 117 85 44 41 23 13 12 BspEI T'CCGG_A Cuts at: 7140 7140 Size: 7477 BspHI T'CATG_A Cuts at: 5366 7096 5366 Size: 1730 5747 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4384 4578 4832 844 Size: 3540 194 254 3489 Fragments arranged by size: 3540 3489 254 194 BsrBI CCG'CTC Cuts at: 1394 1823 5364 1394 Size: 429 3541 3507 Fragments arranged by size: 3541 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2416 3297 5972 6146 65 Size: 1183 1168 881 2675 174 1396 Fragments arranged by size: 2675 1396 1183 1168 881 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3762 6121 6308 1700 Size: 110 1952 2359 187 2869 Fragments arranged by size: 2869 2359 1952 187 110 BsrGI T'GTAC_A Cuts at: 96 4455 96 Size: 4359 3118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7477 BssSI C'ACGA_G Cuts at: 5537 6549 5537 Size: 1012 6465 BstAPI GCAn_nnn'nTGC Cuts at: 794 2517 3405 4357 4535 794 Size: 1723 888 952 178 3736 Fragments arranged by size: 3736 1723 952 888 178 BstBI TT'CG_AA Cuts at: 2007 6281 2007 Size: 4274 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7477 BstXI CCAn_nnnn'nTGG Cuts at: 2312 3442 4246 2312 Size: 1130 804 5543 Fragments arranged by size: 5543 1130 804 BstYI r'GATC_y Cuts at: 1972 2061 2403 3339 3659 3955 4807 5174 5559 Size: 89 342 936 320 296 852 367 385 Cuts at: 5559 5576 7028 7114 7126 7472 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 936 852 385 367 346 342 320 296 89 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2340 3621 4033 513 Size: 552 957 318 1281 412 3957 Fragments arranged by size: 3957 1281 957 552 412 318 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6738 Fragments arranged by size: 6738 683 56 BtsI GCAGTG_nn' Cuts at: 2835 4993 5787 5807 2835 Size: 2158 794 20 4505 Fragments arranged by size: 4505 2158 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7477 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7442 DraI TTT'AAA Cuts at: 3388 4590 4781 3388 Size: 1202 191 6084 Fragments arranged by size: 6084 1202 191 DraIII CAC_nnn'GTG Cuts at: 4025 4025 Size: 7477 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6484 809 Size: 507 5168 1802 Fragments arranged by size: 5168 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3113 4482 4924 Size: 54 1425 50 275 18 1283 1369 442 Cuts at: 4924 5813 7442 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1369 1283 889 442 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4924 1537 Size: 275 3112 4090 Fragments arranged by size: 4090 3112 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2177 3786 4136 4436 5407 1353 Size: 701 123 1609 350 300 971 3423 Fragments arranged by size: 3423 1609 971 701 350 300 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6064 6438 6584 387 Size: 1064 138 4475 374 146 1280 Fragments arranged by size: 4475 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3148 3380 3488 3818 4617 5540 6137 Size: 393 1516 232 108 330 799 923 597 Cuts at: 6137 6924 7192 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1516 923 799 787 597 393 330 268 232 108 EcoNI CCTnn'n_nnAGG Cuts at: 4160 4256 4160 Size: 96 7381 EcoO109I rG'GnC_Cy Cuts at: 2107 2611 2661 3733 4152 2107 Size: 504 50 1072 419 5432 Fragments arranged by size: 5432 1072 504 419 50 EcoRI G'AATT_C Cuts at: 4670 4786 5164 4670 Size: 116 378 6983 Fragments arranged by size: 6983 378 116 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2758 2932 2758 Size: 174 7303 FspI TGC'GCA Cuts at: 5983 5983 Size: 7477 HaeII r_GCGC'y Cuts at: 1678 3082 3256 6624 1678 Size: 1404 174 3368 2531 Fragments arranged by size: 3368 2531 1404 174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2623 2655 2693 2725 3329 Size: 32 135 32 1051 32 38 32 604 Cuts at: 3329 3361 3536 3568 3866 3898 3947 3968 3979 Size: 32 175 32 298 32 49 21 11 Cuts at: 3979 3983 4000 4015 4671 4687 4703 4719 6118 Size: 4 17 15 656 16 16 16 1399 Cuts at: 6118 6150 6192 6224 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1399 1051 656 604 298 175 135 49 42 38 32 32 32 32 32 32 32 32 32 21 17 16 16 16 15 11 4 HincII GTy'rAC Cuts at: 669 1657 1938 3355 4821 5067 669 Size: 988 281 1417 1466 246 3079 Fragments arranged by size: 3079 1466 1417 988 281 246 HindIII A'AGCT_T Cuts at: 748 3010 748 Size: 2262 5215 HpaI GTT'AAC Cuts at: 5067 5067 Size: 7477 KasI G'GCGC_C Cuts at: 3252 3252 Size: 7477 KpnI G_GTAC'C Cuts at: 4802 4802 Size: 7477 MfeI C'AATT_G Cuts at: 5076 5076 Size: 7477 MluI A'CGCG_T Cuts at: 7353 7353 Size: 7477 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2373 4227 6591 6775 1305 Size: 1068 1854 2364 184 2007 Fragments arranged by size: 2364 2007 1854 1068 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3115 10 Size: 54 1425 343 1283 4372 Fragments arranged by size: 4372 1425 1283 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3053 3626 4463 5435 5794 5953 518 Size: 648 1887 573 837 972 359 159 2042 Fragments arranged by size: 2042 1887 972 837 648 573 359 159 MspA1I CmG'CkG Cuts at: 1606 2417 4893 5570 6718 6963 1606 Size: 811 2476 677 1148 245 2120 Fragments arranged by size: 2476 2120 1148 811 677 245 NarI GG'CG_CC Cuts at: 3253 3253 Size: 7477 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5968 Fragments arranged by size: 5968 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7477 NheI G'CTAG_C Cuts at: 1052 4079 1052 Size: 3027 4450 NotI GC'GGCC_GC Cuts at: 4924 4924 Size: 7477 NspI r_CATG'y Cuts at: 1132 1229 4468 4725 4835 6380 1132 Size: 97 3239 257 110 1545 2229 Fragments arranged by size: 3239 2229 1545 257 110 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7477 PciI A'CATG_T Cuts at: 6376 6376 Size: 7477 PflMI CCAn_nnn'nTGG Cuts at: 1108 3126 3892 4218 1108 Size: 2018 766 326 4367 Fragments arranged by size: 4367 2018 766 326 PfoI T'CCnGG_A Cuts at: 4633 4633 Size: 7477 PmeI GTTT'AAAC Cuts at: 4781 4781 Size: 7477 PmlI CAC'GTG Cuts at: 3463 3463 Size: 7477 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5546 5578 7059 7091 4382 Size: 32 1132 32 1481 32 4768 Fragments arranged by size: 4768 1481 1132 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 2661 3733 4152 2107 Size: 554 1072 419 5432 Fragments arranged by size: 5432 1072 554 419 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7477 PsiI TTA'TAA Cuts at: 5047 5047 Size: 7477 PspOMI G'GGCC_C Cuts at: 3044 3044 Size: 7477 PstI C_TGCA'G Cuts at: 830 4575 4829 830 Size: 3745 254 3478 Fragments arranged by size: 3745 3478 254 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7477 PvuII CAG'CTG Cuts at: 2417 2417 Size: 7477 SalI G'TCGA_C Cuts at: 1936 4819 1936 Size: 2883 4594 SanDI GG'GwC_CC Cuts at: 3733 3733 Size: 7477 SapI GCTCTTCn'nnn_ Cuts at: 2177 3786 4436 2177 Size: 1609 650 5218 Fragments arranged by size: 5218 1609 650 SbfI CC_TGCA'GG Cuts at: 4829 4829 Size: 7477 ScaI AGT'ACT Cuts at: 4610 5725 7437 4610 Size: 1115 1712 4650 Fragments arranged by size: 4650 1712 1115 SfcI C'TryA_G Cuts at: 826 1045 4571 4825 5960 6641 6832 826 Size: 219 3526 254 1135 681 191 1471 Fragments arranged by size: 3526 1471 1135 681 254 219 191 SfoI GGC'GCC Cuts at: 3254 3254 Size: 7477 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7477 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7477 SmaI CCC'GGG Cuts at: 1872 4804 1872 Size: 2932 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4427 5581 6482 6744 820 Size: 197 926 2484 1154 901 262 1553 Fragments arranged by size: 2484 1553 1154 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4774 5268 493 Size: 4281 494 2702 Fragments arranged by size: 4281 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7477 SphI G_CATG'C Cuts at: 1132 4725 4835 1132 Size: 3593 110 3774 Fragments arranged by size: 3774 3593 110 SspI AAT'ATT Cuts at: 5 52 5401 5 Size: 47 5349 2081 Fragments arranged by size: 5349 2081 47 StuI AGG'CCT Cuts at: 1517 4115 1517 Size: 2598 4879 StyI C'CwwG_G Cuts at: 513 1065 2022 2325 4683 4916 513 Size: 552 957 303 2358 233 3074 Fragments arranged by size: 3074 2358 957 552 303 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3464 3791 4490 5504 5521 5674 5859 Size: 1255 1884 327 699 1014 17 153 185 Cuts at: 5859 6304 325 Size: 445 1498 Fragments arranged by size: 1884 1498 1255 1014 699 445 327 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3513 4455 4608 Size: 274 80 33 51 1443 1536 942 153 Cuts at: 4608 5723 7435 96 Size: 1115 1712 138 Fragments arranged by size: 1712 1536 1443 1115 942 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2514 2546 4013 4045 4078 4110 7437 7469 2514 Size: 32 1467 32 33 32 3327 32 2522 Fragments arranged by size: 3327 2522 1467 33 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7477 XbaI T'CTAG_A Cuts at: 4813 4813 Size: 7477 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3055 3123 4022 3055 Size: 68 899 6510 Fragments arranged by size: 6510 899 68 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7477 XmaI C'CCGG_G Cuts at: 1870 4802 1870 Size: 2932 4545 XmnI GAAnn'nnTTC Cuts at: 2798 5606 2798 Size: 2808 4669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5285 276 Size: 53 83 186 936 3751 2468 Fragments arranged by size: 3751 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI