(Circular) (Six-base) MAPSORT of: pFN21ASDA1020.seq Check: 9450 from: 1 to: 6574 pFN21ASDA1020 6574 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3852 3852 Size: 6574 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2166 4384 278 Size: 53 83 186 936 630 2218 2468 Fragments arranged by size: 2468 2218 936 630 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2353 3716 4637 6021 6289 1632 Size: 721 1363 921 1384 268 1917 Fragments arranged by size: 1917 1384 1363 921 721 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 3297 3668 5473 6450 1203 Size: 2094 371 1805 977 1327 Fragments arranged by size: 2094 1805 1327 977 371 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 2047 3762 1166 Size: 881 1715 3978 Fragments arranged by size: 3978 1715 881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2354 2440 3411 1184 Size: 1170 86 971 4347 Fragments arranged by size: 4347 1170 971 86 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3413 3445 3413 Size: 32 6542 AlwNI CAG_nnn'CTG Cuts at: 2119 2515 2755 4317 5889 2119 Size: 396 240 1562 1572 2804 Fragments arranged by size: 2804 1572 1562 396 240 ApaI G_GGCC'C Cuts at: 2037 2085 2200 2578 3093 3183 2037 Size: 48 115 378 515 90 5428 Fragments arranged by size: 5428 515 378 115 90 48 ApaLI G'TGCA_C Cuts at: 3767 4631 5787 3767 Size: 864 1156 4554 Fragments arranged by size: 4554 1156 864 ApoI r'AATT_y Cuts at: 1493 3883 4109 4261 6370 1493 Size: 2390 226 152 2109 1697 Fragments arranged by size: 2390 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 2408 3106 3772 3899 1870 Size: 73 465 698 666 127 4545 Fragments arranged by size: 4545 698 666 465 127 73 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2921 2983 3470 3659 3895 Size: 325 217 627 1134 62 487 189 236 Cuts at: 3895 5350 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1134 627 487 325 236 217 189 62 BanII G_rGCy'C Cuts at: 1391 2037 2085 2200 2364 2556 2578 2595 2937 Size: 646 48 115 164 192 22 17 342 Cuts at: 2937 3093 3183 3195 3528 3893 1391 Size: 156 90 12 333 365 4072 Fragments arranged by size: 4072 646 365 342 333 192 164 156 115 90 48 22 17 12 BbeI G_GCGC'C Cuts at: 2987 2987 Size: 6574 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3249 3563 3602 928 Size: 971 1350 314 39 3900 Fragments arranged by size: 3900 1350 971 314 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3005 3039 4765 4799 1903 Size: 34 1068 34 1726 34 3678 Fragments arranged by size: 3678 1726 1068 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 1548 Size: 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2685 2856 2992 Size: 107 122 71 631 605 1013 171 136 Cuts at: 2992 3518 3690 5185 136 Size: 526 172 1495 1525 Fragments arranged by size: 1525 1495 1013 631 605 526 172 171 136 122 107 71 BglII A'GATC_T Cuts at: 2307 6569 2307 Size: 4262 2312 BlpI GC'TnA_GC Cuts at: 2840 3522 3991 2840 Size: 682 469 5423 Fragments arranged by size: 5423 682 469 Bme1580I G_kGCm'C Cuts at: 1790 2037 2085 2200 2578 2924 3093 3183 3771 Size: 247 48 115 378 346 169 90 588 Cuts at: 3771 4635 5791 1790 Size: 864 1156 2573 Fragments arranged by size: 2573 1156 864 588 378 346 247 169 115 90 48 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3498 4305 5263 Size: 416 53 83 399 24 2081 807 958 Cuts at: 5263 442 Size: 1753 Fragments arranged by size: 2081 1753 958 807 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 2966 2999 5234 1239 Size: 1145 582 33 2235 2579 Fragments arranged by size: 2579 2235 1145 582 33 Bpu10I CC'TnA_GC Cuts at: 2969 2969 Size: 6574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2423 4699 5564 5862 2423 Size: 2276 865 298 3135 Fragments arranged by size: 3135 2276 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5237 882 Size: 617 3738 2219 Fragments arranged by size: 3738 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3403 3871 4365 493 Size: 711 2199 468 494 2702 Fragments arranged by size: 2702 2199 711 494 468 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 2984 4381 4763 Size: 53 83 186 936 630 821 1397 382 Cuts at: 4763 5398 275 Size: 635 1451 Fragments arranged by size: 1451 1397 936 821 635 630 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2046 5007 5405 5679 5826 6237 2046 Size: 2961 398 274 147 411 2383 Fragments arranged by size: 2961 2383 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6349 Fragments arranged by size: 6349 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 3128 3227 3230 3233 3491 3494 1186 Size: 1183 759 99 3 3 258 3 4266 Fragments arranged by size: 4266 1183 759 258 99 3 3 3 BseYI C'CCAG_C Cuts at: 2099 2342 2680 2755 2835 2914 3377 3571 3819 Size: 243 338 75 80 79 463 194 248 Cuts at: 3819 5777 6273 2099 Size: 1958 496 2400 Fragments arranged by size: 2400 1958 496 463 338 248 243 194 80 79 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 1163 Size: 573 498 5503 Fragments arranged by size: 5503 573 498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2595 3759 3771 4635 4720 5791 2595 Size: 1164 12 864 85 1071 3378 Fragments arranged by size: 3378 1164 1071 864 85 12 BsmI GAATG_Cn' Cuts at: 3354 4085 4178 3354 Size: 731 93 5750 Fragments arranged by size: 5750 731 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3314 3314 Size: 6574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2085 2200 2364 2556 2578 2595 Size: 399 247 48 115 164 192 22 17 Cuts at: 2595 2924 2937 3093 3183 3195 3528 3759 3771 Size: 329 13 156 90 12 333 231 12 Cuts at: 3771 3893 4635 4720 5791 1391 Size: 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 333 329 247 231 192 164 156 122 115 90 85 48 22 17 13 12 12 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 4463 6193 4463 Size: 1730 4844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3852 3929 844 Size: 3008 77 3489 Fragments arranged by size: 3489 3008 77 BsrBI CCG'CTC Cuts at: 1394 1823 3076 3258 4461 1394 Size: 429 1253 182 1203 3507 Fragments arranged by size: 3507 1253 1203 429 182 BsrDI GCAATG_nn' Cuts at: 65 1248 5069 5243 65 Size: 1183 3821 174 1396 Fragments arranged by size: 3821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2447 3303 5218 5405 1700 Size: 110 637 856 1915 187 2869 Fragments arranged by size: 2869 1915 856 637 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 2555 3590 4634 5646 2555 Size: 1035 1044 1012 3483 Fragments arranged by size: 3483 1044 1035 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2119 3360 3506 794 Size: 1325 1241 146 3862 Fragments arranged by size: 3862 1325 1241 146 BstBI TT'CG_AA Cuts at: 2007 5378 2007 Size: 3371 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstXI CCAn_nnnn'nTGG Cuts at: 2488 2944 2488 Size: 456 6118 BstYI r'GATC_y Cuts at: 1972 2307 2941 3904 4271 4656 4673 6125 6211 Size: 335 634 963 367 385 17 1452 86 Cuts at: 6211 6223 6569 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 963 634 385 367 346 335 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2520 2937 513 Size: 552 957 498 417 4150 Fragments arranged by size: 4150 957 552 498 417 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3726 487 Size: 683 56 2500 3335 Fragments arranged by size: 3335 2500 683 56 BtsI GCAGTG_nn' Cuts at: 2382 3469 4090 4884 4904 2382 Size: 1087 621 794 20 4052 Fragments arranged by size: 4052 1087 794 621 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3616 3651 529 Size: 35 3052 35 3452 Fragments arranged by size: 3452 3052 35 35 DraI TTT'AAA Cuts at: 3878 3878 Size: 6574 DraIII CAC_nnn'GTG Cuts at: 3506 3506 Size: 6574 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3277 4021 4910 Size: 54 1425 50 275 18 1447 744 889 Cuts at: 4910 6539 8 Size: 1629 43 Fragments arranged by size: 1629 1447 1425 889 744 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2999 3417 3435 4504 1353 Size: 1646 418 18 1069 3423 Fragments arranged by size: 3423 1646 1069 418 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5161 5535 5681 387 Size: 1064 138 3572 374 146 1280 Fragments arranged by size: 3572 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2593 2593 Size: 6574 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2353 2384 2966 2999 3716 4637 5234 Size: 393 721 31 582 33 717 921 597 Cuts at: 5234 6021 6289 1239 Size: 787 268 1524 Fragments arranged by size: 1524 921 787 721 717 597 582 393 268 33 31 EcoNI CCTnn'n_nnAGG Cuts at: 2978 2978 Size: 6574 EcoO109I rG'GnC_Cy Cuts at: 2034 2081 2574 2807 3089 3098 3180 3447 3490 Size: 47 493 233 282 9 82 267 43 Cuts at: 3490 3568 2034 Size: 78 5040 Fragments arranged by size: 5040 493 282 267 233 82 78 47 43 9 EcoRI G'AATT_C Cuts at: 3883 4261 3883 Size: 378 6196 FspI TGC'GCA Cuts at: 3252 3399 3848 5080 3252 Size: 147 449 1232 4746 Fragments arranged by size: 4746 1232 449 147 FspAI rTGC'GCAy Cuts at: 3399 3399 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 2987 3291 5721 1678 Size: 1309 304 2430 2531 Fragments arranged by size: 2531 2430 1309 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3311 3343 3479 3511 5215 Size: 32 135 32 1739 32 136 32 1704 Cuts at: 5215 5247 5289 5321 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1739 1704 136 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3918 4164 669 Size: 988 281 1980 246 3079 Fragments arranged by size: 3079 1980 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KasI G'GCGC_C Cuts at: 2983 2983 Size: 6574 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 3668 6450 3668 Size: 2782 3792 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5688 5872 1305 Size: 4383 184 2007 Fragments arranged by size: 4383 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4752 Fragments arranged by size: 4752 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2047 2171 2174 2279 2486 3762 3764 Size: 648 881 124 3 105 207 1276 2 Cuts at: 3764 4532 4891 5050 518 Size: 768 359 159 2042 Fragments arranged by size: 2042 1276 881 768 648 359 207 159 124 105 3 2 MspA1I CmG'CkG Cuts at: 1606 2128 2404 2512 2684 2736 2839 3276 3799 Size: 522 276 108 172 52 103 437 523 Cuts at: 3799 3990 4667 5815 6060 1606 Size: 191 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 523 522 437 276 245 191 172 108 103 52 NaeI GCC'GGC Cuts at: 2449 2449 Size: 6574 NarI GG'CG_CC Cuts at: 2984 2984 Size: 6574 NcoI C'CATG_G Cuts at: 513 1065 2022 2937 513 Size: 552 957 915 4150 Fragments arranged by size: 4150 957 915 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NgoMIV G'CCGG_C Cuts at: 2447 2447 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 3301 3679 3727 3763 3853 3932 5477 Size: 97 2072 378 48 36 90 79 1545 Cuts at: 5477 1132 Size: 2229 Fragments arranged by size: 2229 2072 1545 378 97 90 79 48 36 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6574 PciI A'CATG_T Cuts at: 3297 5473 3297 Size: 2176 4398 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6574 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PmlI CAC'GTG Cuts at: 3403 3403 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2807 3098 3490 3568 2807 Size: 291 392 78 5813 Fragments arranged by size: 5813 392 291 78 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PspOMI G'GGCC_C Cuts at: 2033 2081 2196 2574 3089 3179 2033 Size: 48 115 378 515 90 5428 Fragments arranged by size: 5428 515 378 115 90 48 PstI C_TGCA'G Cuts at: 830 2127 2714 3926 830 Size: 1297 587 1212 3478 Fragments arranged by size: 3478 1297 1212 587 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2128 2404 2684 2839 3799 2128 Size: 276 280 155 960 4903 Fragments arranged by size: 4903 960 280 276 155 SacI G_AGCT'C Cuts at: 2595 2595 Size: 6574 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SanDI GG'GwC_CC Cuts at: 3098 3098 Size: 6574 SapI GCTCTTCn'nnn_ Cuts at: 2999 3417 2999 Size: 418 6156 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SfcI C'TryA_G Cuts at: 826 1045 2123 2710 3922 5057 5738 5929 826 Size: 219 1078 587 1212 1135 681 191 1471 Fragments arranged by size: 1471 1212 1135 1078 681 587 219 191 SfoI GGC'GCC Cuts at: 2985 2985 Size: 6574 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3108 3901 1872 Size: 1236 793 4545 Fragments arranged by size: 4545 1236 793 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 4678 5579 5841 820 Size: 197 926 495 2240 901 262 1553 Fragments arranged by size: 2240 1553 926 901 495 262 197 SnaBI TAC'GTA Cuts at: 493 3871 4365 493 Size: 3378 494 2702 Fragments arranged by size: 3378 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3727 3853 3932 1132 Size: 2595 126 79 3774 Fragments arranged by size: 3774 2595 126 79 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6574 StyI C'CwwG_G Cuts at: 513 1065 2022 2037 2145 2937 3093 3366 4013 Size: 552 957 15 108 792 156 273 647 Cuts at: 4013 513 Size: 3074 Fragments arranged by size: 3074 957 792 647 552 273 156 108 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3517 3535 3833 4601 4618 4771 4956 Size: 1255 1937 18 298 768 17 153 185 Cuts at: 4956 5401 325 Size: 445 1498 Fragments arranged by size: 1937 1498 1255 768 445 298 185 153 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4820 6532 96 Size: 274 80 33 51 1443 2843 1712 138 Fragments arranged by size: 2843 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2791 2823 3603 3635 6534 6566 2791 Size: 32 780 32 2899 32 2799 Fragments arranged by size: 2899 2799 780 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2282 3325 1316 Size: 966 1043 4565 Fragments arranged by size: 4565 1043 966 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3151 3151 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 3106 3899 1870 Size: 1236 793 4545 Fragments arranged by size: 4545 1236 793 XmnI GAAnn'nnTTC Cuts at: 2625 3701 4703 2625 Size: 1076 1002 4496 Fragments arranged by size: 4496 1076 1002 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2164 4382 276 Size: 53 83 186 936 630 2218 2468 Fragments arranged by size: 2468 2218 936 630 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI