Restriction map for
pFN21ASDA1004
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrBI BsrGI BssHII
BssSI BstBI CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FbaI
FseI FspI Hin1I KasI KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 2257 CGCG CG^CG
AccIII 3113 TCCGGA T^CCGGA
AocI 836 CCTCAGG CC^T[ACGT]AGG
AspI 2208 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 2461 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 391 GAGACC GGTCTC(1/5)
BsaWI 3113 TCCGGA [AT]^CCGG[AT]
BseAI 3113 TCCGGA T^CCGGA
BsmI 1921 GAATGC GAATGC(1/-1)
BspEI 3113 TCCGGA T^CCGGA
BsrFI 3075 ACCGGC [GA]^CCGG[CT]
Bst1107I 858 GTATAC GTA^TAC
BstEII 775 GGTGACC G^GT[ACGT]ACC
BstPI 775 GGTGACC G^GT[ACGT]ACC
BstUI 2257 CGCG CG^CG
BstXI 1999 CCAAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 858 GTATAC GTA^TAC
Bsu36I 836 CCTCAGG CC^T[ACGT]AGG
Cfr10I 3075 ACCGGC [GA]^CCGG[CT]
Eco81I 836 CCTCAGG CC^T[ACGT]AGG
EcoNI 1047 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 775 GGTGACC G^GT[ACGT]ACC
EcoRI 237 GAATTC G^AATTC
HindIII 1561 AAGCTT A^AGCTT
HpaI 1644 GTTAAC GTT^AAC
MroI 3113 TCCGGA T^CCGGA
MvnI 2257 CGCG CG^CG
PaeR7I 436 CTCGAG C^TCGAG
PflFI 2208 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 714 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3208 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 593 AGTACT AGT^ACT
SexAI 3087 ACCAGGT A^CC[AT]GGT
SfiI 2460 GGCCATCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2195 CCCGGG CCC^GGG
Tth111I 2208 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 714 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 436 CTCGAG C^TCGAG
XmaCI 2195 CCCGGG C^CCGGG
XmaI 2195 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 46 GGTACC G^GTACC
649 GGTACC
AccI 505 GTAGAC GT^[AC][GT]AC
858 GTATAC
AflIII 512 ACATGT A^C[GA][CT]GT
1855 ACATGT
Aor51HI 1013 AGCGCT AGC^GCT
2264 AGCGCT
Asp700I 16 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1375 GAAGGCCTTC
Asp718I 46 GGTACC G^GTACC
649 GGTACC
AvaI 436 CTCGAG C^[CT]CG[GA]G
2195 CCCGGG
BalI 517 TGGCCA TGG^CCA
1546 TGGCCA
BamHI 267 GGATCC G^GATCC
1317 GGATCC
BcgI 3400 CGAAAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3390 GCAGATCACTCG
BsmBI 1698 GAGACG CGTCTC(1/5)
2663 GAGACG
BsoBI 436 CTCGAG C^[CT]CG[GA]G
2195 CCCGGG
BspLU11I 512 ACATGT A^CATGT
1855 ACATGT
BspMI 1228 ACCTGC ACCTGC(4/8)
3304 GCAGGT
Eco47III 1013 AGCGCT AGC^GCT
2264 AGCGCT
HaeII 1013 AGCGCT [GA]GCGC^[CT]
2264 AGCGCT
HincII 1198 GTCAAC GT[CT]^[GA]AC
1644 GTTAAC
HindII 1198 GTCAAC GT[CT]^[GA]AC
1644 GTTAAC
KpnI 46 GGTACC GGTAC^C
649 GGTACC
MluNI 517 TGGCCA TGG^CCA
1546 TGGCCA
MscI 517 TGGCCA TGG^CCA
1546 TGGCCA
NcoI 365 CCATGG C^CATGG
2312 CCATGG
PpuMI 3010 AGGACCC [GA]G^G[AT]CC[CT]
3044 AGGACCC
PvuII 1661 CAGCTG CAG^CTG
2994 CAGCTG
StuI 1377 AGGCCT AGG^CCT
3141 AGGCCT
XmnI 16 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1375 GAAGGCCTTC
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII