(Circular) (Six-base) MAPSORT of: pFN21ASDA1004.seq Check: 4673 from: 1 to: 8215 pFN21ASDA1004 8215 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6025 278 Size: 53 83 186 936 4489 2468 Fragments arranged by size: 4489 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2529 2882 5558 1937 Size: 592 353 2676 4594 Fragments arranged by size: 4594 2676 592 353 Acc65I G'GTAC_C Cuts at: 2069 2672 5536 2069 Size: 603 2864 4748 Fragments arranged by size: 4748 2864 603 AclI AA'CG_TT Cuts at: 1563 6342 6715 1563 Size: 4779 373 3063 Fragments arranged by size: 4779 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3293 4404 6278 7662 7930 1632 Size: 1661 1111 1874 1384 268 1917 Fragments arranged by size: 1917 1874 1661 1384 1111 268 AfeI AGC'GCT Cuts at: 3038 4289 3038 Size: 1251 6964 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8018 AflIII A'CryG_T Cuts at: 1203 2535 3878 7114 8091 1203 Size: 1332 1343 3236 977 1327 Fragments arranged by size: 3236 1343 1332 1327 977 AgeI A'CCGG_T Cuts at: 7046 7046 Size: 8215 AhdI GACnn_n'nnGTC Cuts at: 6944 6944 Size: 8215 AleI CACnn'nnGTG Cuts at: 1166 2900 4269 1166 Size: 1734 1369 5112 Fragments arranged by size: 5112 1734 1369 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2936 1184 Size: 1752 6463 AlwNI CAG_nnn'CTG Cuts at: 3689 4309 5000 5241 5356 5958 7530 3689 Size: 620 691 241 115 602 1572 4374 Fragments arranged by size: 4374 1572 691 620 602 241 115 ApaLI G'TGCA_C Cuts at: 6272 7428 6272 Size: 1156 7059 ApoI r'AATT_y Cuts at: 1493 2260 3080 3590 5076 5524 5750 5902 8011 Size: 767 820 510 1486 448 226 152 2109 Cuts at: 8011 1493 Size: 1697 Fragments arranged by size: 2109 1697 1486 820 767 510 448 226 152 AseI AT'TA_AT Cuts at: 160 1032 6769 160 Size: 872 5737 1606 Fragments arranged by size: 5737 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8215 AvaI C'yCGr_G Cuts at: 1870 1943 2459 4218 5540 1870 Size: 73 516 1759 1322 4545 Fragments arranged by size: 4545 1759 1322 516 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2664 2697 2664 Size: 33 8182 BamHI G'GATC_C Cuts at: 2290 3340 5545 5912 2290 Size: 1050 2205 367 4593 Fragments arranged by size: 4593 2205 1050 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2672 3853 5536 6991 Size: 325 217 627 282 603 1181 1683 1455 Cuts at: 6991 618 Size: 1842 Fragments arranged by size: 1842 1683 1455 1181 627 603 325 282 217 BanII G_rGCy'C Cuts at: 1391 2832 3320 4452 4678 5319 5534 1391 Size: 1441 488 1132 226 641 215 4072 Fragments arranged by size: 4072 1441 1132 641 488 226 215 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2704 3254 4717 5283 928 Size: 971 805 550 1463 566 3860 Fragments arranged by size: 3860 1463 971 805 566 550 BbvCI CC'TCA_GC Cuts at: 3098 4736 5006 3098 Size: 1638 270 6307 Fragments arranged by size: 6307 1638 270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5402 5412 5436 5446 6406 6440 1903 Size: 34 3465 10 24 10 960 34 3678 Fragments arranged by size: 3678 3465 960 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 6107 7317 6107 Size: 1210 7005 BclI T'GATC_A Cuts at: 1548 1548 Size: 8215 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4490 6826 136 Size: 107 122 71 631 605 2818 2336 1525 Fragments arranged by size: 2818 2336 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8210 8210 Size: 8215 BlpI GC'TnA_GC Cuts at: 5632 5632 Size: 8215 Bme1580I G_kGCm'C Cuts at: 1790 4473 4851 6276 7432 1790 Size: 2683 378 1425 1156 2573 Fragments arranged by size: 2683 2573 1425 1156 378 BmgBI CAC'GTC Cuts at: 1318 2359 4779 1318 Size: 1041 2420 4754 Fragments arranged by size: 4754 2420 1041 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2533 3251 3340 Size: 416 53 83 399 24 1116 718 89 Cuts at: 3340 5946 6904 442 Size: 2606 958 1753 Fragments arranged by size: 2606 1753 1116 958 718 416 399 89 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8215 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2262 2456 4078 2262 Size: 194 1622 6399 Fragments arranged by size: 6399 1622 194 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2765 3530 3570 3968 4999 6875 1239 Size: 1262 264 765 40 398 1031 1876 2579 Fragments arranged by size: 2579 1876 1262 1031 765 398 264 40 Bpu10I CC'TnA_GC Cuts at: 3098 4677 4736 5006 3098 Size: 1579 59 270 6307 Fragments arranged by size: 6307 1579 270 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2257 2816 3196 4846 6340 7205 7503 2257 Size: 559 380 1650 1494 865 298 2969 Fragments arranged by size: 2969 1650 1494 865 559 380 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2408 6878 882 Size: 617 909 4470 2219 Fragments arranged by size: 4470 2219 909 617 BsaAI yAC'GTr Cuts at: 493 1204 5512 6006 493 Size: 711 4308 494 2702 Fragments arranged by size: 4308 2702 711 494 BsaBI GATnn'nnATC Cuts at: 8209 8209 Size: 8215 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6022 6404 7039 275 Size: 53 83 186 936 4489 382 635 1451 Fragments arranged by size: 4489 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5136 6648 7046 7320 7467 7878 5136 Size: 1512 398 274 147 411 5473 Fragments arranged by size: 5473 1512 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2582 2612 1178 Size: 30 165 30 1179 30 6781 Fragments arranged by size: 6781 1179 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 2441 3527 4486 4586 4604 4607 4616 Size: 1252 3 1086 959 100 18 3 9 Cuts at: 4616 4619 4622 4625 4628 4649 4958 1186 Size: 3 3 3 3 21 309 4443 Fragments arranged by size: 4443 1252 1086 959 309 100 21 18 9 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3287 4029 4256 4361 4543 4557 4579 7418 7914 Size: 742 227 105 182 14 22 2839 496 Cuts at: 7914 3287 Size: 3588 Fragments arranged by size: 3588 2839 742 496 227 182 105 22 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 2513 2630 3425 4553 4650 4665 Size: 573 402 375 117 795 1128 97 15 Cuts at: 4665 1163 Size: 4713 Fragments arranged by size: 4713 1128 795 573 402 375 117 97 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5665 6426 7454 1540 Size: 275 186 3664 761 1028 2301 Fragments arranged by size: 3664 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2832 3355 4452 5319 5439 6276 6361 7432 2832 Size: 523 1097 867 120 837 85 1071 3615 Fragments arranged by size: 3615 1097 1071 867 837 523 120 85 BsmI GAATG_Cn' Cuts at: 3950 5726 5819 3950 Size: 1776 93 6346 Fragments arranged by size: 6346 1776 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3715 4680 3715 Size: 965 7250 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2832 3320 3355 4452 4473 4678 4851 Size: 399 1042 488 35 1097 21 205 173 Cuts at: 4851 5319 5439 5534 6276 6361 7432 1391 Size: 468 120 95 742 85 1071 2174 Fragments arranged by size: 2174 1097 1071 1042 742 488 468 399 205 173 120 95 85 35 21 BspEI T'CCGG_A Cuts at: 5136 7878 5136 Size: 2742 5473 BspHI T'CATG_A Cuts at: 6104 7834 6104 Size: 1730 6485 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 5318 5570 844 Size: 2416 2058 252 3489 Fragments arranged by size: 3489 2416 2058 252 BsrBI CCG'CTC Cuts at: 1394 1823 6102 1394 Size: 429 4279 3507 Fragments arranged by size: 4279 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3486 3964 5373 6710 6884 65 Size: 1183 2238 478 1409 1337 174 1396 Fragments arranged by size: 2238 1409 1396 1337 1183 478 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5098 6859 7046 1700 Size: 110 3288 1761 187 2869 Fragments arranged by size: 3288 2869 1761 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8215 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8215 BssSI C'ACGA_G Cuts at: 6275 7287 6275 Size: 1012 7203 BstAPI GCAn_nnn'nTGC Cuts at: 794 3252 4309 5000 794 Size: 2458 1057 691 4009 Fragments arranged by size: 4009 2458 1057 691 BstBI TT'CG_AA Cuts at: 2007 7019 2007 Size: 5012 3203 BstEII G'GTnAC_C Cuts at: 1184 2798 1184 Size: 1614 6601 BstXI CCAn_nnnn'nTGG Cuts at: 4029 4029 Size: 8215 BstYI r'GATC_y Cuts at: 1972 2242 2290 3340 4401 5545 5912 6297 6314 Size: 270 48 1050 1061 1144 367 385 17 Cuts at: 6314 7766 7852 7864 8210 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1144 1061 1050 385 367 346 270 86 48 17 12 BstZ17I GTA'TAC Cuts at: 2883 2883 Size: 8215 Bsu36I CC'TnA_GG Cuts at: 2860 2860 Size: 8215 BtgI C'CryG_G Cuts at: 513 1065 2022 2388 2505 3662 4335 4453 513 Size: 552 957 366 117 1157 673 118 4275 Fragments arranged by size: 4275 1157 957 673 552 366 118 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3745 4161 4296 487 Size: 683 56 2519 416 135 4406 Fragments arranged by size: 4406 2519 683 416 135 56 BtsI GCAGTG_nn' Cuts at: 3159 3437 4537 5239 5277 5731 6525 6545 3159 Size: 278 1100 702 38 454 794 20 4829 Fragments arranged by size: 4829 1100 794 702 454 278 38 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8215 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8180 DraI TTT'AAA Cuts at: 5519 5519 Size: 8215 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7222 809 Size: 507 5906 1802 Fragments arranged by size: 5906 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2540 3569 3602 Size: 54 1425 50 275 18 710 1029 33 Cuts at: 3602 4660 5662 6551 8180 8 Size: 1058 1002 889 1629 43 Fragments arranged by size: 1629 1425 1058 1029 1002 889 710 275 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 5662 1537 Size: 275 3850 4090 Fragments arranged by size: 4090 3850 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 3906 4086 4593 6145 1353 Size: 786 1767 180 507 1552 3423 Fragments arranged by size: 3423 1767 1552 786 507 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6802 7176 7322 387 Size: 1064 138 5213 374 146 1280 Fragments arranged by size: 5213 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2830 4450 5317 2830 Size: 1620 867 5728 Fragments arranged by size: 5728 1620 867 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 2765 3293 3530 3570 3968 4404 Size: 393 869 264 528 237 40 398 436 Cuts at: 4404 4999 6278 6875 7662 7930 1239 Size: 595 1279 597 787 268 1524 Fragments arranged by size: 1524 1279 869 787 597 595 528 436 398 393 268 264 237 40 EcoNI CCTnn'n_nnAGG Cuts at: 3074 3074 Size: 8215 EcoO109I rG'GnC_Cy Cuts at: 2499 3809 4855 5034 5068 2499 Size: 1310 1046 179 34 5646 Fragments arranged by size: 5646 1310 1046 179 34 EcoRI G'AATT_C Cuts at: 2260 5524 5902 2260 Size: 3264 378 4573 Fragments arranged by size: 4573 3264 378 FspI TGC'GCA Cuts at: 6721 6721 Size: 8215 HaeII r_GCGC'y Cuts at: 1678 3040 4291 7362 1678 Size: 1362 1251 3071 2531 Fragments arranged by size: 3071 2531 1362 1251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2377 2409 2792 2824 3955 Size: 32 135 32 805 32 383 32 1131 Cuts at: 3955 3987 4401 4433 5026 5058 5217 5249 5281 Size: 32 414 32 593 32 159 32 32 Cuts at: 5281 6856 6888 6930 6962 1373 Size: 1575 32 42 32 2626 Fragments arranged by size: 2626 1575 1131 805 593 414 383 159 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3223 3669 5559 5805 669 Size: 988 281 1285 446 1890 246 3079 Fragments arranged by size: 3079 1890 1285 988 446 281 246 HindIII A'AGCT_T Cuts at: 748 3584 748 Size: 2836 5379 HpaI GTT'AAC Cuts at: 3669 5805 3669 Size: 2136 6079 KpnI G_GTAC'C Cuts at: 2073 2676 5540 2073 Size: 603 2864 4748 Fragments arranged by size: 4748 2864 603 MfeI C'AATT_G Cuts at: 5814 5814 Size: 8215 MluI A'CGCG_T Cuts at: 8091 8091 Size: 8215 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3012 3105 3405 3865 4883 7329 7513 1305 Size: 1707 93 300 460 1018 2446 184 2007 Fragments arranged by size: 2446 2007 1707 1018 460 300 184 93 MscI TGG'CCA Cuts at: 10 64 1489 1832 2542 3571 10 Size: 54 1425 343 710 1029 4654 Fragments arranged by size: 4654 1425 1029 710 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2381 2900 2939 4184 4269 6173 Size: 648 1191 24 519 39 1245 85 1904 Cuts at: 6173 6532 6691 518 Size: 359 159 2042 Fragments arranged by size: 2042 1904 1245 1191 648 519 359 159 85 39 24 MspA1I CmG'CkG Cuts at: 1606 2227 3686 4365 4705 5019 5631 6308 7456 Size: 621 1459 679 340 314 612 677 1148 Cuts at: 7456 7701 1606 Size: 245 2120 Fragments arranged by size: 2120 1459 1148 679 677 621 612 340 314 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2388 4335 513 Size: 552 957 366 1947 4393 Fragments arranged by size: 4393 1947 957 552 366 NdeI CA'TA_TG Cuts at: 387 387 Size: 8215 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8215 NotI GC'GGCC_GC Cuts at: 5662 5662 Size: 8215 NspI r_CATG'y Cuts at: 1132 1229 2539 3882 5212 5573 7118 1132 Size: 97 1310 1343 1330 361 1545 2229 Fragments arranged by size: 2229 1545 1343 1330 1310 361 97 PasI CC'CwG_GG Cuts at: 1348 4244 1348 Size: 2896 5319 PciI A'CATG_T Cuts at: 2535 3878 7114 2535 Size: 1343 3236 3636 Fragments arranged by size: 3636 3236 1343 PflMI CCAn_nnn'nTGG Cuts at: 1108 2743 1108 Size: 1635 6580 PmeI GTTT'AAAC Cuts at: 5519 5519 Size: 8215 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6284 6316 7797 7829 6284 Size: 32 1481 32 6670 Fragments arranged by size: 6670 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5034 5068 5034 Size: 34 8181 PshAI GACnn'nnGTC Cuts at: 1613 5235 1613 Size: 3622 4593 PsiI TTA'TAA Cuts at: 5785 5785 Size: 8215 PspXI vC'TCGA_Gb Cuts at: 2459 2459 Size: 8215 PstI C_TGCA'G Cuts at: 830 3788 4194 4320 4482 4996 5246 5567 830 Size: 2958 406 126 162 514 250 321 3478 Fragments arranged by size: 3478 2958 514 406 321 250 162 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8215 PvuII CAG'CTG Cuts at: 3686 5019 3686 Size: 1333 6882 SacI G_AGCT'C Cuts at: 2832 4452 5319 2832 Size: 1620 867 5728 Fragments arranged by size: 5728 1620 867 SalI G'TCGA_C Cuts at: 1936 5557 1936 Size: 3621 4594 SbfI CC_TGCA'GG Cuts at: 5567 5567 Size: 8215 ScaI AGT'ACT Cuts at: 2618 6463 8175 2618 Size: 3845 1712 2658 Fragments arranged by size: 3845 2658 1712 SexAI A'CCwGG_T Cuts at: 5110 5110 Size: 8215 SfcI C'TryA_G Cuts at: 826 1045 2730 2889 3207 3307 3558 3784 4190 Size: 219 1685 159 318 100 251 226 406 Cuts at: 4190 4316 4430 4478 4992 5242 5563 6698 7379 Size: 126 114 48 514 250 321 1135 681 Cuts at: 7379 7570 826 Size: 191 1471 Fragments arranged by size: 1685 1471 1135 681 514 406 321 318 251 250 226 219 191 159 126 114 100 48 SfiI GGCCn_nnn'nGGCC Cuts at: 4490 4490 Size: 8215 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8215 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8215 SmaI CCC'GGG Cuts at: 1872 4220 5542 1872 Size: 2348 1322 4545 Fragments arranged by size: 4545 2348 1322 SmlI C'TyrA_G Cuts at: 820 1017 1943 2236 2459 2831 3175 4825 6319 Size: 197 926 293 223 372 344 1650 1494 Cuts at: 6319 7220 7482 820 Size: 901 262 1553 Fragments arranged by size: 1650 1553 1494 926 901 372 344 293 262 223 197 SnaBI TAC'GTA Cuts at: 493 5512 6006 493 Size: 5019 494 2702 Fragments arranged by size: 5019 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8215 SphI G_CATG'C Cuts at: 1132 5573 1132 Size: 4441 3774 SspI AAT'ATT Cuts at: 5 52 6139 5 Size: 47 6087 2081 Fragments arranged by size: 6087 2081 47 StuI AGG'CCT Cuts at: 1517 3402 5166 1517 Size: 1885 1764 4566 Fragments arranged by size: 4566 1885 1764 StyI C'CwwG_G Cuts at: 513 1065 2022 2388 2543 3075 4335 5654 513 Size: 552 957 366 155 532 1260 1319 3074 Fragments arranged by size: 3074 1319 1260 957 552 532 366 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2786 3871 4192 4224 5162 6242 6259 Size: 1255 1206 1085 321 32 938 1080 17 Cuts at: 6259 6412 6597 7042 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1206 1085 1080 938 445 321 185 153 32 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 3045 3562 Size: 274 80 33 51 1443 639 429 517 Cuts at: 3562 6461 8173 96 Size: 2899 1712 138 Fragments arranged by size: 2899 1712 1443 639 517 429 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3332 3364 3538 3570 4445 4477 8175 8207 3332 Size: 32 174 32 875 32 3698 32 3340 Fragments arranged by size: 3698 3340 875 174 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4234 1316 Size: 2918 5297 XbaI T'CTAG_A Cuts at: 5551 5551 Size: 8215 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2063 2663 4859 2063 Size: 600 2196 5419 Fragments arranged by size: 5419 2196 600 XhoI C'TCGA_G Cuts at: 1943 2459 1943 Size: 516 7699 XmaI C'CCGG_G Cuts at: 1870 4218 5540 1870 Size: 2348 1322 4545 Fragments arranged by size: 4545 2348 1322 XmnI GAAnn'nnTTC Cuts at: 2043 3402 6344 2043 Size: 1359 2942 3914 Fragments arranged by size: 3914 2942 1359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6023 276 Size: 53 83 186 936 4489 2468 Fragments arranged by size: 4489 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BfrBI BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI