(Circular) (Six-base) MAPSORT of: pFN21ASDA0997.seq Check: 4120 from: 1 to: 6661 pFN21ASDA0997 6661 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4471 278 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4004 1937 Size: 2067 4594 Acc65I G'GTAC_C Cuts at: 3982 3982 Size: 6661 AclI AA'CG_TT Cuts at: 1563 4788 5161 1563 Size: 3225 373 3063 Fragments arranged by size: 3225 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 3138 3395 4724 6108 6376 1632 Size: 407 1099 257 1329 1384 268 1917 Fragments arranged by size: 1917 1384 1329 1099 407 268 257 AflII C'TTAA_G Cuts at: 820 1017 3799 820 Size: 197 2782 3682 Fragments arranged by size: 3682 2782 197 AflIII A'CryG_T Cuts at: 1203 2641 3851 5560 6537 1203 Size: 1438 1210 1709 977 1327 Fragments arranged by size: 1709 1438 1327 1210 977 AgeI A'CCGG_T Cuts at: 5492 5492 Size: 6661 AhdI GACnn_n'nnGTC Cuts at: 5390 5390 Size: 6661 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6661 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2217 1184 Size: 1033 5628 AlwNI CAG_nnn'CTG Cuts at: 4404 5976 4404 Size: 1572 5089 ApaLI G'TGCA_C Cuts at: 4718 5874 4718 Size: 1156 5505 ApoI r'AATT_y Cuts at: 1493 2822 3970 4196 4348 6457 1493 Size: 1329 1148 226 152 2109 1697 Fragments arranged by size: 2109 1697 1329 1148 226 152 AseI AT'TA_AT Cuts at: 160 1032 5215 160 Size: 872 4183 1606 Fragments arranged by size: 4183 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 AvaI C'yCGr_G Cuts at: 1870 1943 3986 1870 Size: 73 2043 4545 Fragments arranged by size: 4545 2043 73 BamHI G'GATC_C Cuts at: 3991 4358 3991 Size: 367 6294 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3982 5437 618 Size: 325 217 627 2195 1455 1842 Fragments arranged by size: 2195 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3441 3980 1391 Size: 2050 539 4072 Fragments arranged by size: 4072 2050 539 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2370 928 Size: 971 471 5219 Fragments arranged by size: 5219 971 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4852 4886 1903 Size: 34 2915 34 3678 Fragments arranged by size: 3678 2915 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4553 5763 3593 Size: 960 1210 4491 Fragments arranged by size: 4491 1210 960 BclI T'GATC_A Cuts at: 1548 1548 Size: 6661 BfrBI ATG'CAT Cuts at: 3284 3284 Size: 6661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5272 136 Size: 107 122 71 631 605 3600 1525 Fragments arranged by size: 3600 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2765 3445 6656 2765 Size: 680 3211 2770 Fragments arranged by size: 3211 2770 680 BlpI GC'TnA_GC Cuts at: 4078 4078 Size: 6661 Bme1580I G_kGCm'C Cuts at: 1790 4722 5878 1790 Size: 2932 1156 2573 Fragments arranged by size: 2932 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6661 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4392 5350 442 Size: 416 53 83 399 24 2975 958 1753 Fragments arranged by size: 2975 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2281 5321 1239 Size: 1042 3040 2579 Fragments arranged by size: 3040 2579 1042 Bpu10I CC'TnA_GC Cuts at: 2580 2580 Size: 6661 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4786 5651 5949 4786 Size: 865 298 5498 Fragments arranged by size: 5498 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5324 882 Size: 617 3825 2219 Fragments arranged by size: 3825 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3958 4452 493 Size: 711 2754 494 2702 Fragments arranged by size: 2754 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6655 6655 Size: 6661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4468 4850 5485 275 Size: 53 83 186 936 2935 382 635 1451 Fragments arranged by size: 2935 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5094 5492 5766 5913 6324 5094 Size: 398 274 147 411 5431 Fragments arranged by size: 5431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3311 3341 1178 Size: 30 165 30 1908 30 4498 Fragments arranged by size: 4498 1908 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2892 3332 3491 1186 Size: 1706 440 159 4356 Fragments arranged by size: 4356 1706 440 159 BseYI C'CCAG_C Cuts at: 2033 5864 6360 2033 Size: 3831 496 2334 Fragments arranged by size: 3831 2334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2187 2256 2523 2841 3875 1163 Size: 573 451 69 267 318 1034 3949 Fragments arranged by size: 3949 1034 573 451 318 267 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4111 4872 5900 1540 Size: 275 186 2110 761 1028 2301 Fragments arranged by size: 2301 2110 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4722 4807 5878 4722 Size: 85 1071 5505 Fragments arranged by size: 5505 1071 85 BsmI GAATG_Cn' Cuts at: 4172 4265 4172 Size: 93 6568 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3441 3980 4722 4807 5878 1391 Size: 399 1651 539 742 85 1071 2174 Fragments arranged by size: 2174 1651 1071 742 539 399 85 BspEI T'CCGG_A Cuts at: 6324 6324 Size: 6661 BspHI T'CATG_A Cuts at: 2181 4550 6280 2181 Size: 2369 1730 2562 Fragments arranged by size: 2562 2369 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4016 844 Size: 3172 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4548 1394 Size: 429 2725 3507 Fragments arranged by size: 3507 2725 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2328 2488 5156 5330 65 Size: 1183 1080 160 2668 174 1396 Fragments arranged by size: 2668 1396 1183 1080 174 160 BsrFI r'CCGG_y Cuts at: 1700 1810 5305 5492 1700 Size: 110 3495 187 2869 Fragments arranged by size: 3495 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6661 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6661 BssSI C'ACGA_G Cuts at: 4721 5733 4721 Size: 1012 5649 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6661 BstBI TT'CG_AA Cuts at: 2007 5465 2007 Size: 3458 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6661 BstYI r'GATC_y Cuts at: 1972 2765 3445 3991 4358 4743 4760 6212 6298 Size: 793 680 546 367 385 17 1452 86 Cuts at: 6298 6310 6656 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 793 680 546 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5152 Fragments arranged by size: 5152 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5922 Fragments arranged by size: 5922 683 56 BtsI GCAGTG_nn' Cuts at: 4177 4971 4991 4177 Size: 794 20 5847 Fragments arranged by size: 5847 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6626 DraI TTT'AAA Cuts at: 2314 3604 3965 2314 Size: 1290 361 5010 Fragments arranged by size: 5010 1290 361 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5668 809 Size: 507 4352 1802 Fragments arranged by size: 4352 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3640 3915 4108 Size: 54 1425 50 275 18 1810 275 193 Cuts at: 4108 4997 6626 8 Size: 889 1629 43 Fragments arranged by size: 1810 1629 1425 889 275 275 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 1537 Size: 275 2296 4090 Fragments arranged by size: 4090 2296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2410 2547 2958 3114 3489 4591 1353 Size: 1057 137 411 156 375 1102 3423 Fragments arranged by size: 3423 1102 1057 411 375 156 137 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2677 5248 5622 5768 387 Size: 1064 138 1088 2571 374 146 1280 Fragments arranged by size: 2571 1280 1088 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2281 3138 3395 4724 5321 6108 Size: 393 407 242 857 257 1329 597 787 Cuts at: 6108 6376 1239 Size: 268 1524 Fragments arranged by size: 1524 1329 857 787 597 407 393 268 257 242 EcoO109I rG'GnC_Cy Cuts at: 3240 3322 3932 3240 Size: 82 610 5969 Fragments arranged by size: 5969 610 82 EcoRI G'AATT_C Cuts at: 3970 4348 3970 Size: 378 6283 FspI TGC'GCA Cuts at: 5167 5167 Size: 6661 HaeII r_GCGC'y Cuts at: 1678 5808 1678 Size: 4130 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2540 2572 3311 3314 3343 Size: 32 135 32 968 32 739 3 29 Cuts at: 3343 3346 3497 3529 5302 5334 5376 5408 1373 Size: 3 151 32 1773 32 42 32 2626 Fragments arranged by size: 2626 1773 968 739 151 135 42 32 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 4005 4251 669 Size: 988 281 2067 246 3079 Fragments arranged by size: 3079 2067 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6661 HpaI GTT'AAC Cuts at: 4251 4251 Size: 6661 KpnI G_GTAC'C Cuts at: 3986 3986 Size: 6661 MfeI C'AATT_G Cuts at: 2109 4260 2109 Size: 2151 4510 MluI A'CGCG_T Cuts at: 6537 6537 Size: 6661 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5775 5959 1305 Size: 4470 184 2007 Fragments arranged by size: 4470 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3642 3917 10 Size: 54 1425 343 1810 275 2754 Fragments arranged by size: 2754 1810 1425 343 275 54 MslI CAynn'nnrTG Cuts at: 518 1166 4619 4978 5137 518 Size: 648 3453 359 159 2042 Fragments arranged by size: 3453 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3790 4077 4754 5902 6147 1606 Size: 2184 287 677 1148 245 2120 Fragments arranged by size: 2184 2120 1148 677 287 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5152 Fragments arranged by size: 5152 957 552 NdeI CA'TA_TG Cuts at: 387 3678 387 Size: 3291 3370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6661 NotI GC'GGCC_GC Cuts at: 4108 4108 Size: 6661 NsiI A_TGCA'T Cuts at: 3286 3286 Size: 6661 NspI r_CATG'y Cuts at: 1132 1229 2726 3372 3422 3855 4019 5564 1132 Size: 97 1497 646 50 433 164 1545 2229 Fragments arranged by size: 2229 1545 1497 646 433 164 97 50 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6661 PciI A'CATG_T Cuts at: 3851 5560 3851 Size: 1709 4952 PflMI CCAn_nnn'nTGG Cuts at: 1108 2496 1108 Size: 1388 5273 PfoI T'CCnGG_A Cuts at: 2370 2370 Size: 6661 PmeI GTTT'AAAC Cuts at: 3965 3965 Size: 6661 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4730 4762 6243 6275 4730 Size: 32 1481 32 5116 Fragments arranged by size: 5116 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3322 3322 Size: 6661 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6661 PsiI TTA'TAA Cuts at: 4231 4231 Size: 6661 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2593 2625 2593 Size: 32 6629 PstI C_TGCA'G Cuts at: 830 4013 830 Size: 3183 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6661 SalI G'TCGA_C Cuts at: 1936 4003 1936 Size: 2067 4594 SapI GCTCTTCn'nnn_ Cuts at: 3114 3114 Size: 6661 SbfI CC_TGCA'GG Cuts at: 4013 4013 Size: 6661 ScaI AGT'ACT Cuts at: 2200 4909 6621 2200 Size: 2709 1712 2240 Fragments arranged by size: 2709 2240 1712 SfcI C'TryA_G Cuts at: 826 1045 2155 2333 3027 4009 5144 5825 6016 Size: 219 1110 178 694 982 1135 681 191 Cuts at: 6016 826 Size: 1471 Fragments arranged by size: 1471 1135 1110 982 694 681 219 191 178 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6661 SmaI CCC'GGG Cuts at: 1872 3988 1872 Size: 2116 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3799 4765 5666 5928 820 Size: 197 926 1856 966 901 262 1553 Fragments arranged by size: 1856 1553 966 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3958 4452 493 Size: 3465 494 2702 Fragments arranged by size: 3465 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6661 SphI G_CATG'C Cuts at: 1132 4019 1132 Size: 2887 3774 SspI AAT'ATT Cuts at: 5 52 4585 5 Size: 47 4533 2081 Fragments arranged by size: 4533 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6661 StyI C'CwwG_G Cuts at: 513 1065 2022 4100 513 Size: 552 957 2078 3074 Fragments arranged by size: 3074 2078 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4688 4705 4858 5043 5488 325 Size: 1255 3108 17 153 185 445 1498 Fragments arranged by size: 3108 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2198 2735 3262 Size: 274 80 33 51 1443 221 537 527 Cuts at: 3262 4907 6619 96 Size: 1645 1712 138 Fragments arranged by size: 1712 1645 1443 537 527 274 221 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6621 6653 6621 Size: 32 6629 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6661 XbaI T'CTAG_A Cuts at: 3997 3997 Size: 6661 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6661 XmaI C'CCGG_G Cuts at: 1870 3986 1870 Size: 2116 4545 XmnI GAAnn'nnTTC Cuts at: 3021 4790 3021 Size: 1769 4892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4469 276 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI