Restriction map for
pFN21ASDA0992
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI AlwI AlwNI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BciVI BfrI BglI
BlnI BsaBI BsaWI BseAI BsgI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssSI BstAPI BstBI BstXI
ClaI CpoI Eam1105I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HindIII
KasI MamI MfeI MluI MroI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SmlI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1610 GGTACC G^GTACC
AcsI 554 AAATTC [GA]^AATT[CT]
Alw44I 1435 GTGCAC G^TGCAC
Aor51HI 430 AGCGCT AGC^GCT
ApaI 97 GGGCCC GGGCC^C
ApaLI 1435 GTGCAC G^TGCAC
ApoI 554 AAATTC [GA]^AATT[CT]
Asp718I 1610 GGTACC G^GTACC
BalI 630 TGGCCA TGG^CCA
BbrPI 1466 CACGTG CAC^GTG
BbsI 283 GTCTTC GAAGAC(2/6)
BcgI 1912 GCAGCACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 223 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 223 GCTGAGC GC^T[ACGT]AGC
BpuAI 283 GTCTTC GAAGAC(2/6)
BsaAI 1466 CACGTG [CT]AC^GT[GA]
BsiWI 1389 CGTACG C^GTACG
BsmBI 1232 GAGACG CGTCTC(1/5)
BsmI 911 GAATGC GAATGC(1/-1)
BspMI 1036 ACCTGC ACCTGC(4/8)
Bst1107I 1782 GTATAC GTA^TAC
BstEII 1346 GGTTACC G^GT[ACGT]ACC
BstPI 1346 GGTTACC G^GT[ACGT]ACC
BstZ17I 1782 GTATAC GTA^TAC
CelII 223 GCTGAGC GC^T[ACGT]AGC
DraI 970 TTTAAA TTT^AAA
DraIII 1845 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1330 GACTGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 718 GAAGAG CTCTTC(1/4)
Eco47III 430 AGCGCT AGC^GCT
EcoO65I 1346 GGTTACC G^GT[ACGT]ACC
HpaI 642 GTTAAC GTT^AAC
KpnI 1610 GGTACC GGTAC^C
Ksp632I 718 GAAGAG CTCTTC(1/4)
KspI 416 CCGCGG CCGC^GG
MluNI 630 TGGCCA TGG^CCA
MscI 630 TGGCCA TGG^CCA
MslI 1189 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 767 GACTC GAGTC(4/5)
PmaCI 1466 CACGTG CAC^GTG
PmlI 1466 CACGTG CAC^GTG
PshAI 362 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1320 CTGCAG CTGCA^G
PvuII 753 CAGCTG CAG^CTG
SacII 416 CCGCGG CCGC^GG
SalI 668 GTCGAC G^TCGAC
SfcI 1320 CTGCAG C^T[GA][CT]AG
SgrAI 455 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 357 CCCGGG CCC^GGG
SphI 1712 GCATGC GCATG^C
SspI 703 AATATT AAT^ATT
StuI 1204 AGGCCT AGG^CCT
XbaI 1213 TCTAGA T^CTAGA
XmaCI 357 CCCGGG C^CCGGG
XmaI 357 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 668 GTCGAC GT^[AC][GT]AC
1782 GTATAC
Asp700I 1243 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1515 GAACTCATTC
BbvCI 426 CCTCAGC CCTCAGC(-5/-2)
1895 CCTCAGC
BclI 1002 TGATCA T^GATCA
1445 TGATCA
BsaI 800 GGTCTC GGTCTC(1/5)
1175 GGTCTC
BseRI 246 CTCCTC GAGGAG(10/8)
249 CTCCTC
BsiEI 183 CGGCCG CG[GA][CT]^CG
461 CGGCCG
BspLU11I 928 ACATGT A^CATGT
1486 ACATGT
BsrBI 18 CCGCTC CCGCTC(-3/-3)
346 CCGCTC
BssHII 5 GCGCGC G^CGCGC
61 GCGCGC
EagI 183 CGGCCG C^GGCCG
461 CGGCCG
EclXI 183 CGGCCG C^GGCCG
461 CGGCCG
Eco52I 183 CGGCCG C^GGCCG
461 CGGCCG
EcoT14I 485 CCAAGG C^C[AT][AT]GG
1994 CCTTGG
FbaI 1002 TGATCA T^GATCA
1445 TGATCA
HaeII 220 GGCGCT [GA]GCGC^[CT]
430 AGCGCT
MspA1I 416 CCGCGG C[AC]G^C[GT]G
753 CAGCTG
StyI 485 CCAAGG C^C[AT][AT]GG
1994 CCTTGG
XcmI 955 CCAAAGATCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1357 CCAGATCTAAGCTGG
XmnI 1243 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1515 GAACTCATTC
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AocI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII