(Circular) (Six-base) MAPSORT of: pFN21ASDA0992.seq Check: 3480 from: 1 to: 6745 pFN21ASDA0992 6745 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4555 278 Size: 53 83 186 936 3019 2468 Fragments arranged by size: 3019 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2692 3806 4088 1937 Size: 755 1114 282 4594 Fragments arranged by size: 4594 1114 755 282 Acc65I G'GTAC_C Cuts at: 3633 4066 3633 Size: 433 6312 AclI AA'CG_TT Cuts at: 1563 4872 5245 1563 Size: 3309 373 3063 Fragments arranged by size: 3309 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2293 3271 4808 6192 6460 1632 Size: 661 978 1537 1384 268 1917 Fragments arranged by size: 1917 1537 1384 978 661 268 AfeI AGC'GCT Cuts at: 2455 2455 Size: 6745 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6548 AflIII A'CryG_T Cuts at: 1203 2340 2951 3509 5644 6621 1203 Size: 1137 611 558 2135 977 1327 Fragments arranged by size: 2135 1327 1137 977 611 558 AgeI A'CCGG_T Cuts at: 5576 5576 Size: 6745 AhdI GACnn_n'nnGTC Cuts at: 5474 5474 Size: 6745 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6745 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6745 AlwNI CAG_nnn'CTG Cuts at: 4488 6060 4488 Size: 1572 5173 ApaI G_GGCC'C Cuts at: 2124 2124 Size: 6745 ApaLI G'TGCA_C Cuts at: 3458 4802 5958 3458 Size: 1344 1156 4245 Fragments arranged by size: 4245 1344 1156 ApoI r'AATT_y Cuts at: 1493 2577 4054 4280 4432 6541 1493 Size: 1084 1477 226 152 2109 1697 Fragments arranged by size: 2109 1697 1477 1084 226 152 AseI AT'TA_AT Cuts at: 160 1032 5299 160 Size: 872 4267 1606 Fragments arranged by size: 4267 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2252 2374 2380 4070 1870 Size: 73 174 135 122 6 1690 4545 Fragments arranged by size: 4545 1690 174 135 122 73 6 BamHI G'GATC_C Cuts at: 4075 4442 4075 Size: 367 6378 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2332 2887 3633 4066 5521 Size: 325 217 627 545 555 746 433 1455 Cuts at: 5521 618 Size: 1842 Fragments arranged by size: 1842 1455 746 627 555 545 433 325 217 BanII G_rGCy'C Cuts at: 1391 2124 2253 2270 2381 4064 1391 Size: 733 129 17 111 1683 4072 Fragments arranged by size: 4072 1683 733 129 111 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2299 928 Size: 971 400 5374 Fragments arranged by size: 5374 971 400 BbvCI CC'TCA_GC Cuts at: 2450 3919 2450 Size: 1469 5276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3924 3958 4936 4970 1903 Size: 34 1987 34 978 34 3678 Fragments arranged by size: 3678 1987 978 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4637 5847 4637 Size: 1210 5535 BclI T'GATC_A Cuts at: 1548 3025 3468 1548 Size: 1477 443 4825 Fragments arranged by size: 4825 1477 443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5356 136 Size: 107 122 71 631 605 3684 1525 Fragments arranged by size: 3684 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2922 2982 3009 3046 3316 3382 6740 2922 Size: 60 27 37 270 66 3358 2927 Fragments arranged by size: 3358 2927 270 66 60 37 27 BlpI GC'TnA_GC Cuts at: 2247 4162 2247 Size: 1915 4830 Bme1580I G_kGCm'C Cuts at: 1790 2124 2337 3462 4806 5962 1790 Size: 334 213 1125 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 1125 334 213 BmgBI CAC'GTC Cuts at: 1318 2341 3486 1318 Size: 1023 1145 4577 Fragments arranged by size: 4577 1145 1023 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3632 3865 4476 Size: 416 53 83 399 24 2215 233 611 Cuts at: 4476 5434 442 Size: 958 1753 Fragments arranged by size: 2215 1753 958 611 416 399 233 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6745 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2134 2758 3333 3572 5405 1239 Size: 895 624 575 239 1833 2579 Fragments arranged by size: 2579 1833 895 624 575 239 Bpu10I CC'TnA_GC Cuts at: 2450 3919 2450 Size: 1469 5276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4870 5735 6033 4870 Size: 865 298 5582 Fragments arranged by size: 5582 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2829 3204 5408 882 Size: 617 1330 375 2204 2219 Fragments arranged by size: 2219 2204 1330 617 375 BsaAI yAC'GTr Cuts at: 493 1204 3491 4042 4536 493 Size: 711 2287 551 494 2702 Fragments arranged by size: 2702 2287 711 551 494 BsaBI GATnn'nnATC Cuts at: 6739 6739 Size: 6745 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2192 2390 2435 4552 Size: 53 83 186 936 659 198 45 2117 Cuts at: 4552 4934 5569 275 Size: 382 635 1451 Fragments arranged by size: 2117 1451 936 659 635 382 198 186 83 53 45 BsaWI w'CCGG_w Cuts at: 5178 5576 5850 5997 6408 5178 Size: 398 274 147 411 5515 Fragments arranged by size: 5515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2355 2385 2756 2786 1178 Size: 30 165 30 952 30 371 30 5137 Fragments arranged by size: 5137 952 371 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2260 2263 1186 Size: 1074 3 5668 Fragments arranged by size: 5668 1074 3 BseYI C'CCAG_C Cuts at: 2156 3797 5948 6444 2156 Size: 1641 2151 496 2457 Fragments arranged by size: 2457 2151 1641 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2209 2487 4195 4956 5984 1540 Size: 275 186 208 278 1708 761 1028 2301 Fragments arranged by size: 2301 1708 1028 761 278 275 208 186 BsiHKAI G_wGCw'C Cuts at: 3430 3462 3896 4806 4891 5962 3430 Size: 32 434 910 85 1071 4213 Fragments arranged by size: 4213 1071 910 434 85 32 BsiWI C'GTAC_G Cuts at: 3412 3412 Size: 6745 BsmI GAATG_Cn' Cuts at: 2940 4256 4349 2940 Size: 1316 93 5336 Fragments arranged by size: 5336 1316 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3249 3249 Size: 6745 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2253 2270 2337 2381 3430 3462 Size: 399 334 129 17 67 44 1049 32 Cuts at: 3462 3896 4064 4806 4891 5962 1391 Size: 434 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 1049 742 434 399 334 168 129 85 67 44 32 17 BspEI T'CCGG_A Cuts at: 6408 6408 Size: 6745 BspHI T'CATG_A Cuts at: 4634 6364 4634 Size: 1730 5015 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3068 4100 844 Size: 2224 1032 3489 Fragments arranged by size: 3489 2224 1032 BsrBI CCG'CTC Cuts at: 1394 1823 2043 2371 4632 1394 Size: 429 220 328 2261 3507 Fragments arranged by size: 3507 2261 429 328 220 BsrDI GCAATG_nn' Cuts at: 65 1248 5240 5414 65 Size: 1183 3992 174 1396 Fragments arranged by size: 3992 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2143 2479 3497 5389 5576 1700 Size: 110 333 336 1018 1892 187 2869 Fragments arranged by size: 2869 1892 1018 336 333 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6745 BssHII G'CGCG_C Cuts at: 1925 2028 2084 1925 Size: 103 56 6586 Fragments arranged by size: 6586 103 56 BssSI C'ACGA_G Cuts at: 4805 5817 4805 Size: 1012 5733 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6745 BstBI TT'CG_AA Cuts at: 2007 5549 2007 Size: 3542 3203 BstEII G'GTnAC_C Cuts at: 1184 3369 1184 Size: 2185 4560 BstYI r'GATC_y Cuts at: 1972 2922 2982 3009 3046 3316 3382 4075 4442 Size: 950 60 27 37 270 66 693 367 Cuts at: 4442 4827 4844 6296 6382 6394 6740 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 950 693 385 367 346 270 86 66 60 37 27 17 12 BstZ17I GTA'TAC Cuts at: 3807 3807 Size: 6745 Bsu36I CC'TnA_GG Cuts at: 3129 3205 3981 3129 Size: 76 776 5893 Fragments arranged by size: 5893 776 76 BtgI C'CryG_G Cuts at: 513 1065 2134 2261 2316 2425 2439 3751 513 Size: 552 1069 127 55 109 14 1312 3507 Fragments arranged by size: 3507 1312 1069 552 127 109 55 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2086 487 Size: 683 56 860 5146 Fragments arranged by size: 5146 860 683 56 BtsI GCAGTG_nn' Cuts at: 2348 2395 4261 5055 5075 2348 Size: 47 1866 794 20 4018 Fragments arranged by size: 4018 1866 794 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6745 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3346 3381 529 Size: 35 2782 35 3893 Fragments arranged by size: 3893 2782 35 35 DraI TTT'AAA Cuts at: 2995 4049 2995 Size: 1054 5691 DraIII CAC_nnn'GTG Cuts at: 3873 3873 Size: 6745 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3359 5752 809 Size: 507 2043 2393 1802 Fragments arranged by size: 2393 2043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2206 2422 2428 Size: 54 1425 50 275 18 376 216 6 Cuts at: 2428 2484 2653 4192 5081 6710 8 Size: 56 169 1539 889 1629 43 Fragments arranged by size: 1629 1539 1425 889 376 275 216 169 56 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2206 2484 4192 1537 Size: 275 394 278 1708 4090 Fragments arranged by size: 4090 1708 394 278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2736 4675 1353 Size: 1383 1939 3423 Fragments arranged by size: 3423 1939 1383 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2035 5332 5706 5852 387 Size: 1064 138 446 3297 374 146 1280 Fragments arranged by size: 3297 1280 1064 446 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2293 2758 3271 3333 3572 4808 Size: 393 502 159 465 513 62 239 1236 Cuts at: 4808 5405 6192 6460 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1236 787 597 513 502 465 393 268 239 159 62 EcoO109I rG'GnC_Cy Cuts at: 2257 2638 3057 4033 2257 Size: 381 419 976 4969 Fragments arranged by size: 4969 976 419 381 EcoRI G'AATT_C Cuts at: 4054 4432 4054 Size: 378 6367 FspI TGC'GCA Cuts at: 5251 5251 Size: 6745 HaeII r_GCGC'y Cuts at: 1678 2247 2457 5892 1678 Size: 569 210 3435 2531 Fragments arranged by size: 3435 2531 569 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3945 3977 5386 5418 5460 Size: 32 135 32 2373 32 1409 32 42 Cuts at: 5460 5492 1373 Size: 32 2626 Fragments arranged by size: 2626 2373 1409 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2491 2667 2693 3175 4089 4335 Size: 988 281 553 176 26 482 914 246 Cuts at: 4335 669 Size: 3079 Fragments arranged by size: 3079 988 914 553 482 281 246 176 26 HindIII A'AGCT_T Cuts at: 748 748 Size: 6745 HpaI GTT'AAC Cuts at: 2667 4335 2667 Size: 1668 5077 KpnI G_GTAC'C Cuts at: 3637 4070 3637 Size: 433 6312 MfeI C'AATT_G Cuts at: 4344 4344 Size: 6745 MluI A'CGCG_T Cuts at: 6621 6621 Size: 6745 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4000 5859 6043 1305 Size: 2695 1859 184 2007 Fragments arranged by size: 2695 2007 1859 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2655 10 Size: 54 1425 343 823 4100 Fragments arranged by size: 4100 1425 823 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3216 4703 5062 5221 518 Size: 648 2050 1487 359 159 2042 Fragments arranged by size: 2050 2042 1487 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2441 2778 4161 4838 5986 6231 1606 Size: 835 337 1383 677 1148 245 2120 Fragments arranged by size: 2120 1383 1148 835 677 337 245 NaeI GCC'GGC Cuts at: 2145 2481 3499 2145 Size: 336 1018 5391 Fragments arranged by size: 5391 1018 336 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6193 NdeI CA'TA_TG Cuts at: 387 387 Size: 6745 NgoMIV G'CCGG_C Cuts at: 2143 2479 3497 2143 Size: 336 1018 5391 Fragments arranged by size: 5391 1018 336 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6745 NotI GC'GGCC_GC Cuts at: 4192 4192 Size: 6745 NspI r_CATG'y Cuts at: 1132 1229 2955 3513 3739 4103 5648 1132 Size: 97 1726 558 226 364 1545 2229 Fragments arranged by size: 2229 1726 1545 558 364 226 97 PasI CC'CwG_GG Cuts at: 1348 2497 1348 Size: 1149 5596 PciI A'CATG_T Cuts at: 2951 3509 5644 2951 Size: 558 2135 4052 Fragments arranged by size: 4052 2135 558 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6745 PfoI T'CCnGG_A Cuts at: 3310 3310 Size: 6745 PmeI GTTT'AAAC Cuts at: 4049 4049 Size: 6745 PmlI CAC'GTG Cuts at: 3491 3491 Size: 6745 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2775 2807 4814 4846 6327 6359 2775 Size: 32 2007 32 1481 32 3161 Fragments arranged by size: 3161 2007 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2638 3057 4033 2638 Size: 419 976 5350 Fragments arranged by size: 5350 976 419 PshAI GACnn'nnGTC Cuts at: 1613 2389 1613 Size: 776 5969 PsiI TTA'TAA Cuts at: 4315 4315 Size: 6745 PspOMI G'GGCC_C Cuts at: 2120 2120 Size: 6745 PstI C_TGCA'G Cuts at: 830 3347 4097 830 Size: 2517 750 3478 Fragments arranged by size: 3478 2517 750 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6745 PvuII CAG'CTG Cuts at: 2778 2778 Size: 6745 SacII CC_GC'GG Cuts at: 2442 2442 Size: 6745 SalI G'TCGA_C Cuts at: 1936 2691 4087 1936 Size: 755 1396 4594 Fragments arranged by size: 4594 1396 755 SbfI CC_TGCA'GG Cuts at: 4097 4097 Size: 6745 ScaI AGT'ACT Cuts at: 4993 6705 4993 Size: 1712 5033 SfcI C'TryA_G Cuts at: 826 1045 3343 4093 5228 5909 6100 826 Size: 219 2298 750 1135 681 191 1471 Fragments arranged by size: 2298 1471 1135 750 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 SgrAI Cr'CCGG_yG Cuts at: 1700 2479 1700 Size: 779 5966 SmaI CCC'GGG Cuts at: 1872 2382 4072 1872 Size: 510 1690 4545 Fragments arranged by size: 4545 1690 510 SmlI C'TyrA_G Cuts at: 820 1017 1943 4849 5750 6012 820 Size: 197 926 2906 901 262 1553 Fragments arranged by size: 2906 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4042 4536 493 Size: 3549 494 2702 Fragments arranged by size: 3549 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6745 SphI G_CATG'C Cuts at: 1132 3739 4103 1132 Size: 2607 364 3774 Fragments arranged by size: 3774 2607 364 SspI AAT'ATT Cuts at: 5 52 2728 4669 5 Size: 47 2676 1941 2081 Fragments arranged by size: 2676 2081 1941 47 StuI AGG'CCT Cuts at: 1517 3229 1517 Size: 1712 5033 StyI C'CwwG_G Cuts at: 513 1065 2508 4017 4184 513 Size: 552 1443 1509 167 3074 Fragments arranged by size: 3074 1509 1443 552 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3741 3789 3884 4772 4789 4942 5127 Size: 1255 2161 48 95 888 17 153 185 Cuts at: 5127 5572 325 Size: 445 1498 Fragments arranged by size: 2161 1498 1255 888 445 185 153 95 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2808 3146 3845 Size: 274 80 33 51 1443 831 338 699 Cuts at: 3845 4991 6703 96 Size: 1146 1712 138 Fragments arranged by size: 1712 1443 1146 831 699 338 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2355 2387 6705 6737 2355 Size: 32 4318 32 2363 Fragments arranged by size: 4318 2363 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6745 XbaI T'CTAG_A Cuts at: 3236 4081 3236 Size: 845 5900 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2985 3387 2029 Size: 956 402 5387 Fragments arranged by size: 5387 956 402 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6745 XmaI C'CCGG_G Cuts at: 1870 2380 4070 1870 Size: 510 1690 4545 Fragments arranged by size: 4545 1690 510 XmnI GAAnn'nnTTC Cuts at: 3270 3542 4874 3270 Size: 272 1332 5141 Fragments arranged by size: 5141 1332 272 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4553 276 Size: 53 83 186 936 3019 2468 Fragments arranged by size: 3019 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BstXI EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI