(Circular) (Six-base) MAPSORT of: pFN21ASDA0980.seq Check: 4054 from: 1 to: 8875 pFN21ASDA0980 8875 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3187 4218 3187 Size: 1031 7844 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5226 6685 278 Size: 53 83 186 936 3690 1459 2468 Fragments arranged by size: 3690 2468 1459 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 6218 1937 Size: 130 4151 4594 Fragments arranged by size: 4594 4151 130 Acc65I G'GTAC_C Cuts at: 3348 6196 3348 Size: 2848 6027 AclI AA'CG_TT Cuts at: 1563 7002 7375 1563 Size: 5439 373 3063 Fragments arranged by size: 5439 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2794 3049 3524 3599 3629 3695 4397 5313 Size: 1162 255 475 75 30 66 702 916 Cuts at: 5313 5810 5948 5990 6032 6038 6938 8322 8590 Size: 497 138 42 42 6 900 1384 268 Cuts at: 8590 1632 Size: 1917 Fragments arranged by size: 1917 1384 1162 916 900 702 497 475 268 255 138 75 66 42 42 30 6 AfeI AGC'GCT Cuts at: 5172 5172 Size: 8875 AflII C'TTAA_G Cuts at: 820 1017 2132 820 Size: 197 1115 7563 Fragments arranged by size: 7563 1115 197 AflIII A'CryG_T Cuts at: 1203 2398 4550 5245 7774 8751 1203 Size: 1195 2152 695 2529 977 1327 Fragments arranged by size: 2529 2152 1327 1195 977 695 AgeI A'CCGG_T Cuts at: 7706 7706 Size: 8875 AhdI GACnn_n'nnGTC Cuts at: 7604 7604 Size: 8875 AleI CACnn'nnGTG Cuts at: 1166 4891 1166 Size: 3725 5150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4180 4407 5157 1184 Size: 2996 227 750 4902 Fragments arranged by size: 4902 2996 750 227 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3644 3676 5981 6013 3644 Size: 32 2305 32 6506 Fragments arranged by size: 6506 2305 32 32 AlwNI CAG_nnn'CTG Cuts at: 2076 2116 2155 2683 3004 3058 3178 3241 4177 Size: 40 39 528 321 54 120 63 936 Cuts at: 4177 4507 5572 5665 6618 8190 2076 Size: 330 1065 93 953 1572 2761 Fragments arranged by size: 2761 1572 1065 953 936 528 330 321 120 93 63 54 40 39 ApaI G_GGCC'C Cuts at: 3109 4482 3109 Size: 1373 7502 ApaLI G'TGCA_C Cuts at: 6932 8088 6932 Size: 1156 7719 ApoI r'AATT_y Cuts at: 1493 2216 2792 6184 6410 6562 8671 1493 Size: 723 576 3392 226 152 2109 1697 Fragments arranged by size: 3392 2109 1697 723 576 226 152 AseI AT'TA_AT Cuts at: 160 1032 7429 160 Size: 872 6397 1606 Fragments arranged by size: 6397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8875 AvaI C'yCGr_G Cuts at: 1870 1943 2720 2847 4133 4433 4836 5019 6200 Size: 73 777 127 1286 300 403 183 1181 Cuts at: 6200 1870 Size: 4545 Fragments arranged by size: 4545 1286 1181 777 403 300 183 127 73 BamHI G'GATC_C Cuts at: 6205 6572 6205 Size: 367 8508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3348 3453 4156 4988 5030 Size: 325 217 627 1561 105 703 832 42 Cuts at: 5030 5782 5847 6080 6196 7651 618 Size: 752 65 233 116 1455 1842 Fragments arranged by size: 1842 1561 1455 832 752 703 627 325 233 217 116 105 65 42 BanII G_rGCy'C Cuts at: 1391 2579 2712 2823 3109 3123 3642 3690 3791 Size: 1188 133 111 286 14 519 48 101 Cuts at: 3791 4068 4273 4482 4694 4837 5236 5731 6194 Size: 277 205 209 212 143 399 495 463 Cuts at: 6194 1391 Size: 4072 Fragments arranged by size: 4072 1188 519 495 463 399 286 277 212 209 205 143 133 111 101 48 14 BbeI G_GCGC'C Cuts at: 3457 5034 5851 6084 3457 Size: 1577 817 233 6248 Fragments arranged by size: 6248 1577 817 233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2238 2283 2745 2832 5226 928 Size: 971 339 45 462 87 2394 4577 Fragments arranged by size: 4577 2394 971 462 339 87 45 BbvCI CC'TCA_GC Cuts at: 3465 4233 5741 3465 Size: 768 1508 6599 Fragments arranged by size: 6599 1508 768 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7066 7100 1903 Size: 34 5129 34 3678 Fragments arranged by size: 5129 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6767 7977 6767 Size: 1210 7665 BclI T'GATC_A Cuts at: 1548 2995 1548 Size: 1447 7428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3831 4545 4604 Size: 107 122 71 631 605 2159 714 59 Cuts at: 4604 7486 136 Size: 2882 1525 Fragments arranged by size: 2882 2159 1525 714 631 605 122 107 71 59 BglII A'GATC_T Cuts at: 3045 8870 3045 Size: 5825 3050 BlpI GC'TnA_GC Cuts at: 2449 3179 3362 3732 5819 6292 2449 Size: 730 183 370 2087 473 5032 Fragments arranged by size: 5032 2087 730 473 370 183 Bme1580I G_kGCm'C Cuts at: 1790 3109 4375 4482 4550 4991 5787 6936 8092 Size: 1319 1266 107 68 441 796 1149 1156 Cuts at: 8092 1790 Size: 2573 Fragments arranged by size: 2573 1319 1266 1156 1149 796 441 107 68 BmgBI CAC'GTC Cuts at: 1318 3448 1318 Size: 2130 6745 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5507 6606 7564 Size: 416 53 83 399 24 4090 1099 958 Cuts at: 7564 442 Size: 1753 Fragments arranged by size: 4090 1753 1099 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5020 5020 Size: 8875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2290 2480 2696 2926 2944 3113 3263 Size: 926 125 190 216 230 18 169 150 Cuts at: 3263 3287 3326 3359 3458 3617 3641 3843 3950 Size: 24 39 33 99 159 24 202 107 Cuts at: 3950 4058 4079 4325 4379 4385 4841 4851 4999 Size: 108 21 246 54 6 456 10 148 Cuts at: 4999 5057 5084 5195 5258 5294 5594 7535 1239 Size: 58 27 111 63 36 300 1941 2579 Fragments arranged by size: 2579 1941 926 456 300 246 230 216 202 190 169 159 150 148 125 111 108 107 99 63 58 54 39 36 33 27 24 24 21 18 10 6 Bpu10I CC'TnA_GC Cuts at: 2057 2367 2669 3465 4140 4233 5741 2057 Size: 310 302 796 675 93 1508 5191 Fragments arranged by size: 5191 1508 796 675 310 302 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2807 3674 5720 7000 7865 8163 2807 Size: 867 2046 1280 865 298 3519 Fragments arranged by size: 3519 2046 1280 867 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2877 3926 4281 5153 7538 882 Size: 617 1378 1049 355 872 2385 2219 Fragments arranged by size: 2385 2219 1378 1049 872 617 355 BsaAI yAC'GTr Cuts at: 493 1204 4553 6025 6172 6666 493 Size: 711 3349 1472 147 494 2702 Fragments arranged by size: 3349 2702 1472 711 494 147 BsaBI GATnn'nnATC Cuts at: 8869 8869 Size: 8875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3454 4869 5031 5223 Size: 53 83 186 936 1921 1415 162 192 Cuts at: 5223 5848 6081 6682 7064 7699 275 Size: 625 233 601 382 635 1451 Fragments arranged by size: 1921 1451 1415 936 635 625 601 382 233 192 186 162 83 53 BsaWI w'CCGG_w Cuts at: 7308 7706 7980 8127 8538 7308 Size: 398 274 147 411 7645 Fragments arranged by size: 7645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3281 3311 3332 3362 3644 Size: 30 165 30 1878 30 21 30 282 Cuts at: 3644 3674 3795 3825 4430 4460 4466 4496 4542 Size: 30 121 30 605 30 6 30 46 Cuts at: 4542 4572 5030 5060 1178 Size: 30 458 30 4993 Fragments arranged by size: 4993 1878 605 458 282 165 121 46 30 30 30 30 30 30 30 30 30 30 21 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2906 3482 3668 4076 4322 4451 4454 4457 Size: 1720 576 186 408 246 129 3 3 Cuts at: 4457 4646 5549 5591 5936 6080 1186 Size: 189 903 42 345 144 3981 Fragments arranged by size: 3981 1720 903 576 408 345 246 189 186 144 129 42 3 3 BseYI C'CCAG_C Cuts at: 2121 2644 3826 3924 4264 4852 4881 5472 5757 Size: 523 1182 98 340 588 29 591 285 Cuts at: 5757 8078 8574 2121 Size: 2321 496 2422 Fragments arranged by size: 2422 2321 1182 591 588 523 496 340 285 98 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3654 4150 4945 5741 1163 Size: 573 1918 496 795 796 4297 Fragments arranged by size: 4297 1918 796 795 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4917 6325 7086 8114 1540 Size: 275 186 2916 1408 761 1028 2301 Fragments arranged by size: 2916 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2504 2942 3123 3642 3690 4068 4972 5436 5731 Size: 438 181 519 48 378 904 464 295 Cuts at: 5731 6052 6936 7021 8092 2504 Size: 321 884 85 1071 3287 Fragments arranged by size: 3287 1071 904 884 519 464 438 378 321 295 181 85 48 BsmI GAATG_Cn' Cuts at: 5645 6386 6479 5645 Size: 741 93 8041 Fragments arranged by size: 8041 741 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2460 2763 4860 2460 Size: 303 2097 6475 Fragments arranged by size: 6475 2097 303 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2504 2579 2712 2823 2942 3109 3123 Size: 399 714 75 133 111 119 167 14 Cuts at: 3123 3642 3690 3791 4068 4273 4375 4482 4550 Size: 519 48 101 277 205 102 107 68 Cuts at: 4550 4694 4837 4972 4991 5236 5436 5731 5787 Size: 144 143 135 19 245 200 295 56 Cuts at: 5787 6052 6194 6936 7021 8092 1391 Size: 265 142 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 714 519 399 295 277 265 245 205 200 167 144 143 142 135 133 119 111 107 102 101 85 75 68 56 48 19 14 BspEI T'CCGG_A Cuts at: 8538 8538 Size: 8875 BspHI T'CATG_A Cuts at: 2815 3123 6764 8494 2815 Size: 308 3641 1730 3196 Fragments arranged by size: 3641 3196 1730 308 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3187 3711 3883 4218 6012 6230 844 Size: 2343 524 172 335 1794 218 3489 Fragments arranged by size: 3489 2343 1794 524 335 218 172 BsrBI CCG'CTC Cuts at: 1394 1823 2898 3658 4280 4588 5902 6762 1394 Size: 429 1075 760 622 308 1314 860 3507 Fragments arranged by size: 3507 1314 1075 860 760 622 429 308 BsrDI GCAATG_nn' Cuts at: 65 1248 7370 7544 65 Size: 1183 6122 174 1396 Fragments arranged by size: 6122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2361 3768 4912 7519 7706 1700 Size: 110 551 1407 1144 2607 187 2869 Fragments arranged by size: 2869 2607 1407 1144 551 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8875 BssHII G'CGCG_C Cuts at: 1925 4405 1925 Size: 2480 6395 BssSI C'ACGA_G Cuts at: 2927 4070 6935 7947 2927 Size: 1143 2865 1012 3855 Fragments arranged by size: 3855 2865 1143 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 4507 5815 794 Size: 1361 2352 1308 3854 Fragments arranged by size: 3854 2352 1361 1308 BstBI TT'CG_AA Cuts at: 2007 7679 2007 Size: 5672 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8875 BstXI CCAn_nnnn'nTGG Cuts at: 4599 4893 4599 Size: 294 8581 BstYI r'GATC_y Cuts at: 1972 2622 3045 6205 6572 6957 6974 8426 8512 Size: 650 423 3160 367 385 17 1452 86 Cuts at: 8512 8524 8870 1972 Size: 12 346 1977 Fragments arranged by size: 3160 1977 1452 650 423 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3917 4907 5735 3917 Size: 990 828 7057 Fragments arranged by size: 7057 990 828 BtgI C'CryG_G Cuts at: 513 1065 2022 2085 4126 4459 4720 4886 5675 Size: 552 957 63 2041 333 261 166 789 Cuts at: 5675 513 Size: 3713 Fragments arranged by size: 3713 2041 957 789 552 333 261 166 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5795 487 Size: 683 56 4569 3567 Fragments arranged by size: 4569 3567 683 56 BtsI GCAGTG_nn' Cuts at: 4565 5596 6391 7185 7205 4565 Size: 1031 795 794 20 6235 Fragments arranged by size: 6235 1031 795 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8840 DraI TTT'AAA Cuts at: 5394 6179 5394 Size: 785 8090 DraIII CAC_nnn'GTG Cuts at: 3632 4181 5062 6043 3632 Size: 549 881 981 6464 Fragments arranged by size: 6464 981 881 549 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3140 7882 809 Size: 507 1824 4742 1802 Fragments arranged by size: 4742 1824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2350 3006 4537 Size: 54 1425 50 275 18 520 656 1531 Cuts at: 4537 4589 4914 5799 6322 7211 8840 8 Size: 52 325 885 523 889 1629 43 Fragments arranged by size: 1629 1531 1425 889 885 656 523 520 325 275 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4914 6322 1537 Size: 275 3102 1408 4090 Fragments arranged by size: 4090 3102 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2484 2634 2813 2960 3251 4956 5145 Size: 675 456 150 179 147 291 1705 189 Cuts at: 5145 5892 5904 6805 1353 Size: 747 12 901 3423 Fragments arranged by size: 3423 1705 901 747 675 456 291 189 179 150 147 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3404 4253 5930 7462 7836 7982 Size: 1064 138 1815 849 1677 1532 374 146 Cuts at: 7982 387 Size: 1280 Fragments arranged by size: 1815 1677 1532 1280 1064 849 374 146 138 EcoICRI GAG'CTC Cuts at: 3121 3640 3688 4066 5729 3121 Size: 519 48 378 1663 6267 Fragments arranged by size: 6267 1663 519 378 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2290 2480 2696 2794 2926 2944 Size: 393 533 125 190 216 98 132 18 Cuts at: 2944 3049 3113 3263 3287 3326 3359 3458 3524 Size: 105 64 150 24 39 33 99 66 Cuts at: 3524 3599 3617 3629 3641 3695 3843 3950 4058 Size: 75 18 12 12 54 148 107 108 Cuts at: 4058 4079 4325 4379 4385 4397 4841 4851 4999 Size: 21 246 54 6 12 444 10 148 Cuts at: 4999 5057 5084 5195 5258 5294 5313 5594 5810 Size: 58 27 111 63 36 19 281 216 Cuts at: 5810 5948 5990 6032 6038 6938 7535 8322 8590 Size: 138 42 42 6 900 597 787 268 Cuts at: 8590 1239 Size: 1524 Fragments arranged by size: 1524 900 787 597 533 444 393 281 268 246 216 216 190 150 148 148 138 132 125 111 108 107 105 99 98 75 66 64 63 58 54 54 42 42 39 36 33 27 24 21 19 18 18 12 12 12 10 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 3524 3647 4436 4732 5576 5648 3524 Size: 123 789 296 844 72 6751 Fragments arranged by size: 6751 844 789 296 123 72 EcoO109I rG'GnC_Cy Cuts at: 2884 3001 3106 3238 3592 3700 3921 4096 4322 Size: 117 105 132 354 108 221 175 226 Cuts at: 4322 4479 4504 5082 2884 Size: 157 25 578 6677 Fragments arranged by size: 6677 578 354 226 221 175 157 132 117 108 105 25 EcoRI G'AATT_C Cuts at: 2792 6184 6562 2792 Size: 3392 378 5105 Fragments arranged by size: 5105 3392 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2801 2801 Size: 8875 FseI GG_CCGG'CC Cuts at: 4916 4916 Size: 8875 FspI TGC'GCA Cuts at: 7381 7381 Size: 8875 HaeII r_GCGC'y Cuts at: 1678 2449 3457 5034 5174 5851 6084 8022 1678 Size: 771 1008 1577 140 677 233 1938 2531 Fragments arranged by size: 2531 1938 1577 1008 771 677 233 140 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3281 3313 3795 3827 4466 Size: 32 135 32 1709 32 482 32 639 Cuts at: 4466 4498 4724 4756 5453 5485 7516 7548 7590 Size: 32 226 32 697 32 2031 32 42 Cuts at: 7590 7622 1373 Size: 32 2626 Fragments arranged by size: 2626 2031 1709 697 639 482 226 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 5562 6219 6465 669 Size: 988 281 268 3356 657 246 3079 Fragments arranged by size: 3356 3079 988 657 281 268 246 HindIII A'AGCT_T Cuts at: 748 2135 3551 748 Size: 1387 1416 6072 Fragments arranged by size: 6072 1416 1387 HpaI GTT'AAC Cuts at: 2206 5562 6465 2206 Size: 3356 903 4616 Fragments arranged by size: 4616 3356 903 KasI G'GCGC_C Cuts at: 3453 5030 5847 6080 3453 Size: 1577 817 233 6248 Fragments arranged by size: 6248 1577 817 233 KpnI G_GTAC'C Cuts at: 3352 6200 3352 Size: 2848 6027 MfeI C'AATT_G Cuts at: 6474 6474 Size: 8875 MluI A'CGCG_T Cuts at: 2398 8751 2398 Size: 6353 2522 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2336 4329 4428 6024 7989 8173 1305 Size: 1031 1993 99 1596 1965 184 2007 Fragments arranged by size: 2007 1993 1965 1596 1031 184 99 MscI TGG'CCA Cuts at: 10 64 1489 1832 3008 4539 10 Size: 54 1425 343 1176 1531 4346 Fragments arranged by size: 4346 1531 1425 1176 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3128 4891 5555 6833 7192 7351 518 Size: 648 1962 1763 664 1278 359 159 2042 Fragments arranged by size: 2042 1962 1763 1278 664 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2076 2155 2300 2545 2661 3208 3928 4111 Size: 470 79 145 245 116 547 720 183 Cuts at: 4111 4128 4357 4461 4814 5671 5863 5884 6291 Size: 17 229 104 353 857 192 21 407 Cuts at: 6291 6968 8116 8361 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 857 720 677 547 470 407 353 245 245 229 192 183 145 116 104 79 21 17 NaeI GCC'GGC Cuts at: 2363 4914 2363 Size: 2551 6324 NarI GG'CG_CC Cuts at: 3454 5031 5848 6081 3454 Size: 1577 817 233 6248 Fragments arranged by size: 6248 1577 817 233 NcoI C'CATG_G Cuts at: 513 1065 2022 5675 513 Size: 552 957 3653 3713 Fragments arranged by size: 3713 3653 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8875 NgoMIV G'CCGG_C Cuts at: 2361 4912 2361 Size: 2551 6324 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8875 NotI GC'GGCC_GC Cuts at: 6322 6322 Size: 8875 NspI r_CATG'y Cuts at: 1132 1229 4750 5249 6233 7778 1132 Size: 97 3521 499 984 1545 2229 Fragments arranged by size: 3521 2229 1545 984 499 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8875 PciI A'CATG_T Cuts at: 5245 7774 5245 Size: 2529 6346 PflMI CCAn_nnn'nTGG Cuts at: 1108 4616 1108 Size: 3508 5367 PmeI GTTT'AAAC Cuts at: 6179 6179 Size: 8875 PmlI CAC'GTG Cuts at: 4553 4553 Size: 8875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3644 3676 6944 6976 8457 8489 3644 Size: 32 3268 32 1481 32 4030 Fragments arranged by size: 4030 3268 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2884 3001 3238 3592 3700 3921 4322 5082 2884 Size: 117 237 354 108 221 401 760 6677 Fragments arranged by size: 6677 760 401 354 237 221 117 108 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8875 PsiI TTA'TAA Cuts at: 6445 6445 Size: 8875 PspOMI G'GGCC_C Cuts at: 3105 4478 3105 Size: 1373 7502 PspXI vC'TCGA_Gb Cuts at: 4433 4433 Size: 8875 PstI C_TGCA'G Cuts at: 830 2172 2550 2594 3462 3588 3708 4104 4116 Size: 1342 378 44 868 126 120 396 12 Cuts at: 4116 4215 4386 5353 5670 6227 830 Size: 99 171 967 317 557 3478 Fragments arranged by size: 3478 1342 967 868 557 396 378 317 171 126 120 99 44 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8875 PvuII CAG'CTG Cuts at: 2076 2155 2300 2545 3208 3928 4111 4357 5671 Size: 79 145 245 663 720 183 246 1314 Cuts at: 5671 5884 2076 Size: 213 5067 Fragments arranged by size: 5067 1314 720 663 246 245 213 183 145 79 SacI G_AGCT'C Cuts at: 3123 3642 3690 4068 5731 3123 Size: 519 48 378 1663 6267 Fragments arranged by size: 6267 1663 519 378 48 SacII CC_GC'GG Cuts at: 4129 4462 4129 Size: 333 8542 SalI G'TCGA_C Cuts at: 1936 6217 1936 Size: 4281 4594 SanDI GG'GwC_CC Cuts at: 4322 4322 Size: 8875 SapI GCTCTTCn'nnn_ Cuts at: 2634 4956 5892 2634 Size: 2322 936 5617 Fragments arranged by size: 5617 2322 936 SbfI CC_TGCA'GG Cuts at: 3462 3708 4104 6227 3462 Size: 246 396 2123 6110 Fragments arranged by size: 6110 2123 396 246 ScaI AGT'ACT Cuts at: 5359 7123 8835 5359 Size: 1764 1712 5399 Fragments arranged by size: 5399 1764 1712 SexAI A'CCwGG_T Cuts at: 2880 5715 6039 2880 Size: 2835 324 5716 Fragments arranged by size: 5716 2835 324 SfcI C'TryA_G Cuts at: 826 1045 2068 2168 2546 2590 3458 3521 3584 Size: 219 1023 100 378 44 868 63 63 Cuts at: 3584 3704 4100 4112 4211 4382 4946 5349 5666 Size: 120 396 12 99 171 564 403 317 Cuts at: 5666 6223 7358 8039 8230 826 Size: 557 1135 681 191 1471 Fragments arranged by size: 1471 1135 1023 868 681 564 557 403 396 378 317 219 191 171 120 100 99 63 63 44 12 SfiI GGCCn_nnn'nGGCC Cuts at: 4604 4604 Size: 8875 SfoI GGC'GCC Cuts at: 3455 5032 5849 6082 3455 Size: 1577 817 233 6248 Fragments arranged by size: 6248 1577 817 233 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8875 SmaI CCC'GGG Cuts at: 1872 4838 5021 6202 1872 Size: 2966 183 1181 4545 Fragments arranged by size: 4545 2966 1181 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 2720 2786 3689 4433 5699 Size: 197 926 189 588 66 903 744 1266 Cuts at: 5699 6979 7880 8142 820 Size: 1280 901 262 1553 Fragments arranged by size: 1553 1280 1266 926 903 901 744 588 262 197 189 66 SnaBI TAC'GTA Cuts at: 493 6172 6666 493 Size: 5679 494 2702 Fragments arranged by size: 5679 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8875 SphI G_CATG'C Cuts at: 1132 4750 6233 1132 Size: 3618 1483 3774 Fragments arranged by size: 3774 3618 1483 SrfI GCCC'GGGC Cuts at: 5021 5021 Size: 8875 SspI AAT'ATT Cuts at: 5 52 6799 5 Size: 47 6747 2081 Fragments arranged by size: 6747 2081 47 StuI AGG'CCT Cuts at: 1517 3744 5003 5414 1517 Size: 2227 1259 411 4978 Fragments arranged by size: 4978 2227 1259 411 StyI C'CwwG_G Cuts at: 513 1065 2022 3936 4296 5675 6314 513 Size: 552 957 1914 360 1379 639 3074 Fragments arranged by size: 3074 1914 1379 957 639 552 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4184 5006 5034 6902 6919 7072 7257 Size: 1255 2604 822 28 1868 17 153 185 Cuts at: 7257 7702 325 Size: 445 1498 Fragments arranged by size: 2604 1868 1498 1255 822 445 185 153 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5357 7121 8833 Size: 274 80 33 51 1443 3380 1764 1712 Cuts at: 8833 96 Size: 138 Fragments arranged by size: 3380 1764 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3731 3763 4542 4574 5030 5062 8835 8867 3731 Size: 32 779 32 456 32 3773 32 3739 Fragments arranged by size: 3773 3739 779 456 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5225 1316 Size: 3909 4966 XbaI T'CTAG_A Cuts at: 2833 6211 2833 Size: 3378 5497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4178 5170 5848 4178 Size: 992 678 7205 Fragments arranged by size: 7205 992 678 XhoI C'TCGA_G Cuts at: 1943 2720 4433 1943 Size: 777 1713 6385 Fragments arranged by size: 6385 1713 777 XmaI C'CCGG_G Cuts at: 1870 4836 5019 6200 1870 Size: 2966 183 1181 4545 Fragments arranged by size: 4545 2966 1181 183 XmnI GAAnn'nnTTC Cuts at: 6114 7004 6114 Size: 890 7985 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5224 6683 276 Size: 53 83 186 936 3690 1459 2468 Fragments arranged by size: 3690 2468 1459 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI NruI NsiI PacI PfoI PsrI RsrII SwaI