(Circular) (Six-base) MAPSORT of: pFN21ASDA0977.seq Check: 8703 from: 1 to: 8227 pFN21ASDA0977 8227 bp With 182 enzymes: SgfI * July 22, 2011 10:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6037 278 Size: 53 83 186 936 4501 2468 Fragments arranged by size: 4501 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5570 1937 Size: 3633 4594 Acc65I G'GTAC_C Cuts at: 5548 5548 Size: 8227 AclI AA'CG_TT Cuts at: 1563 6354 6727 1563 Size: 4791 373 3063 Fragments arranged by size: 4791 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 3195 4989 5060 6290 7674 7942 1632 Size: 477 1086 1794 71 1230 1384 268 1917 Fragments arranged by size: 1917 1794 1384 1230 1086 477 268 71 AflII C'TTAA_G Cuts at: 820 1017 4069 4891 820 Size: 197 3052 822 4156 Fragments arranged by size: 4156 3052 822 197 AflIII A'CryG_T Cuts at: 1203 7126 8103 1203 Size: 5923 977 1327 Fragments arranged by size: 5923 1327 977 AgeI A'CCGG_T Cuts at: 7058 7058 Size: 8227 AhdI GACnn_n'nnGTC Cuts at: 6956 6956 Size: 8227 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8227 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3303 1184 Size: 2119 6108 AlwNI CAG_nnn'CTG Cuts at: 2132 5970 7542 2132 Size: 3838 1572 2817 Fragments arranged by size: 3838 2817 1572 ApaI G_GGCC'C Cuts at: 3680 3680 Size: 8227 ApaLI G'TGCA_C Cuts at: 2286 3283 4170 6284 7440 2286 Size: 997 887 2114 1156 3073 Fragments arranged by size: 3073 2114 1156 997 887 ApoI r'AATT_y Cuts at: 1493 2354 3082 4103 4219 4657 4691 5222 5287 Size: 861 728 1021 116 438 34 531 65 Cuts at: 5287 5536 5762 5914 8023 1493 Size: 249 226 152 2109 1697 Fragments arranged by size: 2109 1697 1021 861 728 531 438 249 226 152 116 65 34 AseI AT'TA_AT Cuts at: 160 1032 2475 6781 160 Size: 872 1443 4306 1606 Fragments arranged by size: 4306 1606 1443 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8227 AvaI C'yCGr_G Cuts at: 1870 1943 4701 5552 1870 Size: 73 2758 851 4545 Fragments arranged by size: 4545 2758 851 73 BamHI G'GATC_C Cuts at: 5557 5924 5557 Size: 367 7860 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 4118 5060 5548 7003 Size: 325 217 627 296 2035 942 488 1455 Cuts at: 7003 618 Size: 1842 Fragments arranged by size: 2035 1842 1455 942 627 488 325 296 217 BanII G_rGCy'C Cuts at: 1391 2908 3680 4577 5546 1391 Size: 1517 772 897 969 4072 Fragments arranged by size: 4072 1517 969 897 772 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2501 3738 5048 5210 928 Size: 971 602 1237 1310 162 3945 Fragments arranged by size: 3945 1310 1237 971 602 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5388 5422 6418 6452 1903 Size: 34 3451 34 996 34 3678 Fragments arranged by size: 3678 3451 996 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5035 6119 7329 5035 Size: 1084 1210 5933 Fragments arranged by size: 5933 1210 1084 BclI T'GATC_A Cuts at: 1548 4408 1548 Size: 2860 5367 BfrBI ATG'CAT Cuts at: 2522 4420 2522 Size: 1898 6329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6838 136 Size: 107 122 71 631 605 5166 1525 Fragments arranged by size: 5166 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8222 8222 Size: 8227 BlpI GC'TnA_GC Cuts at: 3062 5644 3062 Size: 2582 5645 Bme1580I G_kGCm'C Cuts at: 1790 2290 2794 3287 3329 3680 4174 4563 5065 Size: 500 504 493 42 351 494 389 502 Cuts at: 5065 6288 7444 1790 Size: 1223 1156 2573 Fragments arranged by size: 2573 1223 1156 504 502 500 494 493 389 351 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8227 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3912 4557 5958 Size: 416 53 83 399 24 2495 645 1401 Cuts at: 5958 6916 442 Size: 958 1753 Fragments arranged by size: 2495 1753 1401 958 645 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8227 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2922 3328 2922 Size: 406 7821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2079 3183 5166 6887 1239 Size: 840 1104 1983 1721 2579 Fragments arranged by size: 2579 1983 1721 1104 840 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 5062 6352 7217 7515 4081 Size: 981 1290 865 298 4793 Fragments arranged by size: 4793 1290 981 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2096 2769 3811 6890 882 Size: 617 597 673 1042 3079 2219 Fragments arranged by size: 3079 2219 1042 673 617 597 BsaAI yAC'GTr Cuts at: 493 1204 5524 6018 493 Size: 711 4320 494 2702 Fragments arranged by size: 4320 2702 711 494 BsaBI GATnn'nnATC Cuts at: 8221 8221 Size: 8227 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 6034 6416 7051 Size: 53 83 186 936 519 3982 382 635 Cuts at: 7051 275 Size: 1451 Fragments arranged by size: 3982 1451 936 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4870 6660 7058 7332 7479 7890 4870 Size: 1790 398 274 147 411 5207 Fragments arranged by size: 5207 1790 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4870 4900 1178 Size: 30 165 30 3467 30 4505 Fragments arranged by size: 4505 3467 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3245 4971 1186 Size: 2059 1726 4442 Fragments arranged by size: 4442 2059 1726 BseYI C'CCAG_C Cuts at: 2056 4727 7430 7926 2056 Size: 2671 2703 496 2357 Fragments arranged by size: 2703 2671 2357 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3942 5256 1163 Size: 573 2206 1314 4134 Fragments arranged by size: 4134 2206 1314 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2032 5677 6438 7466 1540 Size: 275 186 31 3645 761 1028 2301 Fragments arranged by size: 3645 2301 1028 761 275 186 31 BsiHKAI G_wGCw'C Cuts at: 2290 3287 4174 4577 6288 6373 7444 2290 Size: 997 887 403 1711 85 1071 3073 Fragments arranged by size: 3073 1711 1071 997 887 403 85 BsmI GAATG_Cn' Cuts at: 5738 5831 5738 Size: 93 8134 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2290 2794 2908 3287 3329 3680 4174 Size: 399 500 504 114 379 42 351 494 Cuts at: 4174 4563 4577 5065 5546 6288 6373 7444 1391 Size: 389 14 488 481 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 500 494 488 481 399 389 379 351 114 85 42 14 BspEI T'CCGG_A Cuts at: 4870 7890 4870 Size: 3020 5207 BspHI T'CATG_A Cuts at: 4444 6116 7846 4444 Size: 1672 1730 4825 Fragments arranged by size: 4825 1730 1672 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3038 4618 5582 844 Size: 2194 1580 964 3489 Fragments arranged by size: 3489 2194 1580 964 BsrBI CCG'CTC Cuts at: 1394 1823 6114 1394 Size: 429 4291 3507 Fragments arranged by size: 4291 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3538 3637 6722 6896 65 Size: 1183 2290 99 3085 174 1396 Fragments arranged by size: 3085 2290 1396 1183 174 99 BsrFI r'CCGG_y Cuts at: 1700 1810 6871 7058 1700 Size: 110 5061 187 2869 Fragments arranged by size: 5061 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8227 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8227 BssSI C'ACGA_G Cuts at: 4302 6287 7299 4302 Size: 1985 1012 5230 Fragments arranged by size: 5230 1985 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4558 794 Size: 3764 4463 BstBI TT'CG_AA Cuts at: 2007 7031 2007 Size: 5024 3203 BstEII G'GTnAC_C Cuts at: 1184 5437 1184 Size: 4253 3974 BstXI CCAn_nnnn'nTGG Cuts at: 3001 3301 4360 5170 3001 Size: 300 1059 810 6058 Fragments arranged by size: 6058 1059 810 300 BstYI r'GATC_y Cuts at: 1972 4141 4159 5557 5924 6309 6326 7778 7864 Size: 2169 18 1398 367 385 17 1452 86 Cuts at: 7864 7876 8222 1972 Size: 12 346 1977 Fragments arranged by size: 2169 1977 1452 1398 385 367 346 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 3228 3339 3228 Size: 111 8116 BtgI C'CryG_G Cuts at: 513 1065 2022 3561 4590 513 Size: 552 957 1539 1029 4150 Fragments arranged by size: 4150 1539 1029 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2508 2681 487 Size: 683 56 1282 173 6033 Fragments arranged by size: 6033 1282 683 173 56 BtsI GCAGTG_nn' Cuts at: 2148 2909 3053 3285 3287 4224 4511 5743 6537 Size: 761 144 232 2 937 287 1232 794 Cuts at: 6537 6557 2148 Size: 20 3818 Fragments arranged by size: 3818 1232 937 794 761 287 232 144 20 2 ClaI AT'CG_AT Cuts at: 1554 2322 1554 Size: 768 7459 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3520 3555 529 Size: 35 2956 35 5201 Fragments arranged by size: 5201 2956 35 35 DraI TTT'AAA Cuts at: 2406 4696 5531 2406 Size: 2290 835 5102 Fragments arranged by size: 5102 2290 835 DraIII CAC_nnn'GTG Cuts at: 3611 4774 5159 3611 Size: 1163 385 6679 Fragments arranged by size: 6679 1163 385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7234 809 Size: 507 5918 1802 Fragments arranged by size: 5918 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 5515 5674 Size: 54 1425 50 275 18 199 3486 159 Cuts at: 5674 6563 8192 8 Size: 889 1629 43 Fragments arranged by size: 3486 1629 1425 889 275 199 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2029 5674 1537 Size: 275 217 3645 4090 Fragments arranged by size: 4090 3645 275 217 EarI CTCTTCn'nnn_ Cuts at: 1353 2128 2891 3031 3387 4500 4748 6157 1353 Size: 775 763 140 356 1113 248 1409 3423 Fragments arranged by size: 3423 1409 1113 775 763 356 248 140 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6814 7188 7334 387 Size: 1064 138 5225 374 146 1280 Fragments arranged by size: 5225 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4575 4575 Size: 8227 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2109 3183 3195 4989 5060 5166 Size: 393 447 30 1074 12 1794 71 106 Cuts at: 5166 6290 6887 7674 7942 1239 Size: 1124 597 787 268 1524 Fragments arranged by size: 1794 1524 1124 1074 787 597 447 393 268 106 71 30 12 EcoRI G'AATT_C Cuts at: 3082 4103 5287 5536 5914 3082 Size: 1021 1184 249 378 5395 Fragments arranged by size: 5395 1184 1021 378 249 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4163 4163 Size: 8227 FspI TGC'GCA Cuts at: 6733 6733 Size: 8227 HaeII r_GCGC'y Cuts at: 1678 7374 1678 Size: 5696 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2497 2529 2588 Size: 32 135 32 743 32 150 32 59 Cuts at: 2588 2617 2620 2649 3221 3253 3503 3535 3806 Size: 29 3 29 572 32 250 32 271 Cuts at: 3806 3838 4367 4399 4870 4902 5303 5335 6868 Size: 32 529 32 471 32 401 32 1533 Cuts at: 6868 6900 6942 6974 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1533 743 572 529 471 401 271 250 150 135 59 42 32 32 32 32 32 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2677 3649 5571 5817 669 Size: 988 281 739 972 1922 246 3079 Fragments arranged by size: 3079 1922 988 972 739 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8227 HpaI GTT'AAC Cuts at: 5817 5817 Size: 8227 KpnI G_GTAC'C Cuts at: 5552 5552 Size: 8227 MfeI C'AATT_G Cuts at: 5826 5826 Size: 8227 MluI A'CGCG_T Cuts at: 8103 8103 Size: 8227 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2364 2400 2862 2892 5200 5330 7341 7525 Size: 1059 36 462 30 2308 130 2011 184 Cuts at: 7525 1305 Size: 2007 Fragments arranged by size: 2308 2011 2007 1059 462 184 130 36 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6405 Fragments arranged by size: 6405 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2999 3147 3803 4179 4443 4449 5330 Size: 648 1833 148 656 376 264 6 881 Cuts at: 5330 6185 6544 6703 518 Size: 855 359 159 2042 Fragments arranged by size: 2042 1833 881 855 656 648 376 359 264 159 148 6 MspA1I CmG'CkG Cuts at: 1606 2099 2129 2336 4787 5643 6320 7468 7713 Size: 493 30 207 2451 856 677 1148 245 Cuts at: 7713 1606 Size: 2120 Fragments arranged by size: 2451 2120 1148 856 677 493 245 207 30 NcoI C'CATG_G Cuts at: 513 1065 2022 3561 4590 513 Size: 552 957 1539 1029 4150 Fragments arranged by size: 4150 1539 1029 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8227 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8227 NotI GC'GGCC_GC Cuts at: 5674 5674 Size: 8227 NsiI A_TGCA'T Cuts at: 2524 4422 2524 Size: 1898 6329 NspI r_CATG'y Cuts at: 1132 1229 2522 3073 4420 4424 5585 7130 1132 Size: 97 1293 551 1347 4 1161 1545 2229 Fragments arranged by size: 2229 1545 1347 1293 1161 551 97 4 PasI CC'CwG_GG Cuts at: 1348 3260 3329 1348 Size: 1912 69 6246 Fragments arranged by size: 6246 1912 69 PciI A'CATG_T Cuts at: 7126 7126 Size: 8227 PflMI CCAn_nnn'nTGG Cuts at: 1108 3188 5243 5312 1108 Size: 2080 2055 69 4023 Fragments arranged by size: 4023 2080 2055 69 PfoI T'CCnGG_A Cuts at: 2961 2961 Size: 8227 PmeI GTTT'AAAC Cuts at: 5531 5531 Size: 8227 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3034 3066 5326 5358 6296 6328 7809 7841 3034 Size: 32 2260 32 938 32 1481 32 3420 Fragments arranged by size: 3420 2260 1481 938 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8227 PsiI TTA'TAA Cuts at: 4338 5797 4338 Size: 1459 6768 PspOMI G'GGCC_C Cuts at: 3676 3676 Size: 8227 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3177 3209 3177 Size: 32 8195 PstI C_TGCA'G Cuts at: 830 3060 3079 4786 5579 830 Size: 2230 19 1707 793 3478 Fragments arranged by size: 3478 2230 1707 793 19 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8227 PvuII CAG'CTG Cuts at: 2099 2129 2336 4787 2099 Size: 30 207 2451 5539 Fragments arranged by size: 5539 2451 207 30 SacI G_AGCT'C Cuts at: 4577 4577 Size: 8227 SalI G'TCGA_C Cuts at: 1936 5569 1936 Size: 3633 4594 SapI GCTCTTCn'nnn_ Cuts at: 4500 4500 Size: 8227 SbfI CC_TGCA'GG Cuts at: 5579 5579 Size: 8227 ScaI AGT'ACT Cuts at: 6475 8187 6475 Size: 1712 6515 SfcI C'TryA_G Cuts at: 826 1045 2139 3056 3075 4200 4782 5109 5575 Size: 219 1094 917 19 1125 582 327 466 Cuts at: 5575 6710 7391 7582 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1125 1094 917 681 582 466 327 219 191 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8227 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8227 SmaI CCC'GGG Cuts at: 1872 5554 1872 Size: 3682 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4069 4096 4891 5077 6331 7232 Size: 197 926 2126 27 795 186 1254 901 Cuts at: 7232 7494 820 Size: 262 1553 Fragments arranged by size: 2126 1553 1254 926 901 795 262 197 186 27 SnaBI TAC'GTA Cuts at: 493 5524 6018 493 Size: 5031 494 2702 Fragments arranged by size: 5031 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8227 SphI G_CATG'C Cuts at: 1132 4424 5585 1132 Size: 3292 1161 3774 Fragments arranged by size: 3774 3292 1161 SspI AAT'ATT Cuts at: 5 52 3448 4678 6151 5 Size: 47 3396 1230 1473 2081 Fragments arranged by size: 3396 2081 1473 1230 47 StuI AGG'CCT Cuts at: 1517 2971 1517 Size: 1454 6773 StyI C'CwwG_G Cuts at: 513 1065 2022 2079 3526 3561 4359 4590 5666 Size: 552 957 57 1447 35 798 231 1076 Cuts at: 5666 513 Size: 3074 Fragments arranged by size: 3074 1447 1076 957 798 552 231 57 35 SwaI ATTT'AAAT Cuts at: 4696 4696 Size: 8227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2905 4943 6254 6271 6424 6609 7054 Size: 1255 1325 2038 1311 17 153 185 445 Cuts at: 7054 325 Size: 1498 Fragments arranged by size: 2038 1498 1325 1311 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6473 8185 96 Size: 274 80 33 51 1443 4496 1712 138 Fragments arranged by size: 4496 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8187 8219 8187 Size: 32 8195 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8227 XbaI T'CTAG_A Cuts at: 5563 5563 Size: 8227 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8227 XmaI C'CCGG_G Cuts at: 1870 5552 1870 Size: 3682 4545 XmnI GAAnn'nnTTC Cuts at: 3252 6356 3252 Size: 3104 5123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6035 276 Size: 53 83 186 936 4501 2468 Fragments arranged by size: 4501 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI