(Circular) (Six-base) MAPSORT of: pFN21ASDA0967.seq Check: 5628 from: 1 to: 9463 pFN21ASDA0967 9463 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2739 2749 3755 3887 2739 Size: 10 1006 132 8315 Fragments arranged by size: 8315 1006 132 10 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3420 7273 278 Size: 53 83 186 936 1884 3853 2468 Fragments arranged by size: 3853 2468 1884 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4611 6806 1937 Size: 2674 2195 4594 Fragments arranged by size: 4594 2674 2195 Acc65I G'GTAC_C Cuts at: 6784 6784 Size: 9463 AclI AA'CG_TT Cuts at: 1563 7590 7963 1563 Size: 6027 373 3063 Fragments arranged by size: 6027 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2367 2498 2529 2785 3395 5955 7526 8910 Size: 735 131 31 256 610 2560 1571 1384 Cuts at: 8910 9178 1632 Size: 268 1917 Fragments arranged by size: 2560 1917 1571 1384 735 610 268 256 131 31 AfeI AGC'GCT Cuts at: 2906 2906 Size: 9463 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9266 AflIII A'CryG_T Cuts at: 1203 4617 8362 9339 1203 Size: 3414 3745 977 1327 Fragments arranged by size: 3745 3414 1327 977 AgeI A'CCGG_T Cuts at: 8294 8294 Size: 9463 AhdI GACnn_n'nnGTC Cuts at: 8192 8192 Size: 9463 AleI CACnn'nnGTG Cuts at: 1166 2627 1166 Size: 1461 8002 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2909 1184 Size: 1725 7738 AlwNI CAG_nnn'CTG Cuts at: 2808 3588 3747 4964 5097 6511 7206 8778 2808 Size: 780 159 1217 133 1414 695 1572 3493 Fragments arranged by size: 3493 1572 1414 1217 780 695 159 133 ApaI G_GGCC'C Cuts at: 2151 3740 4383 2151 Size: 1589 643 7231 Fragments arranged by size: 7231 1589 643 ApaLI G'TGCA_C Cuts at: 2498 4656 6373 6694 7520 8676 2498 Size: 2158 1717 321 826 1156 3285 Fragments arranged by size: 3285 2158 1717 1156 826 321 ApoI r'AATT_y Cuts at: 1493 3121 4885 6772 6998 7150 9259 1493 Size: 1628 1764 1887 226 152 2109 1697 Fragments arranged by size: 2109 1887 1764 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 8017 160 Size: 872 6985 1606 Fragments arranged by size: 6985 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9463 AvaI C'yCGr_G Cuts at: 1870 1943 2106 2121 3835 4796 4830 5431 6171 Size: 73 163 15 1714 961 34 601 740 Cuts at: 6171 6788 1870 Size: 617 4545 Fragments arranged by size: 4545 1714 961 740 617 601 163 73 34 15 AvrII C'CTAG_G Cuts at: 5744 5744 Size: 9463 BamHI G'GATC_C Cuts at: 5818 6793 7160 5818 Size: 975 367 8121 Fragments arranged by size: 8121 975 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3648 3664 4078 4585 5384 Size: 325 217 627 1861 16 414 507 799 Cuts at: 5384 5710 6784 8239 618 Size: 326 1074 1455 1842 Fragments arranged by size: 1861 1842 1455 1074 799 627 507 414 326 325 217 16 BanII G_rGCy'C Cuts at: 1391 2151 3740 4222 4383 4641 4797 5154 5166 Size: 760 1589 482 161 258 156 357 12 Cuts at: 5166 6178 6419 6782 1391 Size: 1012 241 363 4072 Fragments arranged by size: 4072 1589 1012 760 482 363 357 258 241 161 156 12 BbeI G_GCGC'C Cuts at: 3652 3652 Size: 9463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2355 2486 2853 4040 4486 4830 6090 Size: 971 456 131 367 1187 446 344 1260 Cuts at: 6090 928 Size: 4301 Fragments arranged by size: 4301 1260 1187 971 456 446 367 344 131 BbvCI CC'TCA_GC Cuts at: 4915 4915 Size: 9463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2669 2703 4037 4071 6183 6217 7654 Size: 34 732 34 1334 34 2112 34 1437 Cuts at: 7654 7688 1903 Size: 34 3678 Fragments arranged by size: 3678 2112 1437 1334 732 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7355 8565 7355 Size: 1210 8253 BclI T'GATC_A Cuts at: 1548 2314 6450 1548 Size: 766 4136 4561 Fragments arranged by size: 4561 4136 766 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4468 5102 6163 Size: 107 122 71 631 605 2796 634 1061 Cuts at: 6163 6707 8074 136 Size: 544 1367 1525 Fragments arranged by size: 2796 1525 1367 1061 634 631 605 544 122 107 71 BglII A'GATC_T Cuts at: 2997 9458 2997 Size: 6461 3002 BlpI GC'TnA_GC Cuts at: 6880 6880 Size: 9463 Bme1580I G_kGCm'C Cuts at: 1790 2151 2502 2986 3740 4081 4383 4660 6244 Size: 361 351 484 754 341 302 277 1584 Cuts at: 6244 6377 6698 7524 8680 1790 Size: 133 321 826 1156 2573 Fragments arranged by size: 2573 1584 1156 826 754 484 361 351 341 321 302 277 133 BmgBI CAC'GTC Cuts at: 1318 2427 4618 1318 Size: 1109 2191 6163 Fragments arranged by size: 6163 2191 1109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3034 3500 4018 Size: 416 53 83 399 24 1617 466 518 Cuts at: 4018 4257 4401 7194 8152 442 Size: 239 144 2793 958 1753 Fragments arranged by size: 2793 1753 1617 958 518 466 416 399 239 144 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9463 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3625 6250 3625 Size: 2625 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2204 2882 3871 4622 4733 4759 5053 Size: 815 150 678 989 751 111 26 294 Cuts at: 5053 5138 5463 5673 6181 6220 6332 8123 1239 Size: 85 325 210 508 39 112 1791 2579 Fragments arranged by size: 2579 1791 989 815 751 678 508 325 294 210 150 112 111 85 39 26 Bpu10I CC'TnA_GC Cuts at: 4915 4968 5778 4915 Size: 53 810 8600 Fragments arranged by size: 8600 810 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2555 3185 4986 5118 5550 5592 7588 8453 8751 Size: 630 1801 132 432 42 1996 865 298 Cuts at: 8751 2555 Size: 3267 Fragments arranged by size: 3267 1996 1801 865 630 432 298 132 42 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 4576 4709 4745 4793 5553 8126 Size: 617 531 2546 133 36 48 760 2573 Cuts at: 8126 882 Size: 2219 Fragments arranged by size: 2573 2546 2219 760 617 531 133 48 36 BsaAI yAC'GTr Cuts at: 493 1204 6760 7254 493 Size: 711 5556 494 2702 Fragments arranged by size: 5556 2702 711 494 BsaBI GATnn'nnATC Cuts at: 5191 9457 5191 Size: 4266 5197 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3417 3649 3788 7270 Size: 53 83 186 936 1884 232 139 3482 Cuts at: 7270 7652 8287 275 Size: 382 635 1451 Fragments arranged by size: 3482 1884 1451 936 635 382 232 186 139 83 53 BsaWI w'CCGG_w Cuts at: 4860 7896 8294 8568 8715 9126 4860 Size: 3036 398 274 147 411 5197 Fragments arranged by size: 5197 3036 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2648 2678 2702 2732 3866 Size: 30 165 30 1245 30 24 30 1134 Cuts at: 3866 3896 4757 4787 5136 5166 1178 Size: 30 861 30 349 30 5475 Fragments arranged by size: 5475 1245 1134 861 349 165 30 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2474 2789 3617 3704 3874 3971 4731 Size: 1021 267 315 828 87 170 97 760 Cuts at: 4731 4903 4917 5417 1186 Size: 172 14 500 5232 Fragments arranged by size: 5232 1021 828 760 500 315 267 172 170 97 87 14 BseYI C'CCAG_C Cuts at: 2985 3162 3479 3740 4074 4257 4768 8666 9162 Size: 177 317 261 334 183 511 3898 496 Cuts at: 9162 2985 Size: 3286 Fragments arranged by size: 3898 3286 511 496 334 317 261 183 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2407 2711 2720 2919 2939 4274 6043 Size: 573 671 304 9 199 20 1335 1769 Cuts at: 6043 6169 1163 Size: 126 4457 Fragments arranged by size: 4457 1769 1335 671 573 304 199 126 20 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6711 6913 7674 8702 1540 Size: 275 186 4710 202 761 1028 2301 Fragments arranged by size: 4710 2301 1028 761 275 202 186 BsiHKAI G_wGCw'C Cuts at: 2502 2520 2571 2898 4094 4660 4763 5154 6377 Size: 18 51 327 1196 566 103 391 1223 Cuts at: 6377 6698 7524 7609 8680 2502 Size: 321 826 85 1071 3285 Fragments arranged by size: 3285 1223 1196 1071 826 566 391 327 321 103 85 51 18 BsmI GAATG_Cn' Cuts at: 6974 7067 6974 Size: 93 9370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2502 2520 2571 2898 2986 3740 Size: 399 361 351 18 51 327 88 754 Cuts at: 3740 4081 4094 4222 4383 4641 4660 4763 4797 Size: 341 13 128 161 258 19 103 34 Cuts at: 4797 5154 5166 6178 6244 6377 6419 6698 6782 Size: 357 12 1012 66 133 42 279 84 Cuts at: 6782 7524 7609 8680 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1012 754 742 399 361 357 351 341 327 279 258 161 133 128 103 88 85 84 66 51 42 34 19 18 13 12 BspEI T'CCGG_A Cuts at: 4860 9126 4860 Size: 4266 5197 BspHI T'CATG_A Cuts at: 7352 9082 7352 Size: 1730 7733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2739 2749 3755 3882 3887 6653 6818 844 Size: 1895 10 1006 127 5 2766 165 3489 Fragments arranged by size: 3489 2766 1895 1006 165 127 10 5 BsrBI CCG'CTC Cuts at: 1394 1823 7350 1394 Size: 429 5527 3507 Fragments arranged by size: 5527 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3261 5842 7958 8132 65 Size: 1183 2013 2581 2116 174 1396 Fragments arranged by size: 2581 2116 2013 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6524 8107 8294 1700 Size: 110 4714 1583 187 2869 Fragments arranged by size: 4714 2869 1583 187 110 BsrGI T'GTAC_A Cuts at: 96 5201 6606 96 Size: 5105 1405 2953 Fragments arranged by size: 5105 2953 1405 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9463 BssSI C'ACGA_G Cuts at: 7523 8535 7523 Size: 1012 8451 BstAPI GCAn_nnn'nTGC Cuts at: 794 2734 3344 4690 794 Size: 1940 610 1346 5567 Fragments arranged by size: 5567 1940 1346 610 BstBI TT'CG_AA Cuts at: 2007 8267 2007 Size: 6260 3203 BstEII G'GTnAC_C Cuts at: 1184 5075 1184 Size: 3891 5572 BstXI CCAn_nnnn'nTGG Cuts at: 2228 4726 4783 5968 2228 Size: 2498 57 1185 5723 Fragments arranged by size: 5723 2498 1185 57 BstYI r'GATC_y Cuts at: 1972 2319 2970 2997 3186 5538 5818 6793 7160 Size: 347 651 27 189 2352 280 975 367 Cuts at: 7160 7545 7562 9014 9100 9112 9458 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2352 1977 1452 975 651 385 367 347 346 280 189 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 3720 4526 6279 6593 3720 Size: 806 1753 314 6590 Fragments arranged by size: 6590 1753 806 314 BtgI C'CryG_G Cuts at: 513 1065 2022 2853 3814 3846 4803 6055 6108 Size: 552 957 831 961 32 957 1252 53 Cuts at: 6108 6747 513 Size: 639 3229 Fragments arranged by size: 3229 1252 961 957 957 831 639 552 53 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2904 487 Size: 683 56 1678 7046 Fragments arranged by size: 7046 1678 683 56 BtsI GCAGTG_nn' Cuts at: 3598 6979 7773 7793 3598 Size: 3381 794 20 5268 Fragments arranged by size: 5268 3381 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9428 DraI TTT'AAA Cuts at: 3006 3174 6767 3006 Size: 168 3593 5702 Fragments arranged by size: 5702 3593 168 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8470 809 Size: 507 7154 1802 Fragments arranged by size: 7154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4167 5103 5958 Size: 54 1425 50 275 18 2337 936 855 Cuts at: 5958 6221 6522 6708 6910 7799 9428 8 Size: 263 301 186 202 889 1629 43 Fragments arranged by size: 2337 1629 1425 936 889 855 301 275 263 202 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6708 6910 1537 Size: 275 4896 202 4090 Fragments arranged by size: 4896 4090 275 202 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2459 2697 2736 2804 3104 3369 3855 Size: 669 437 238 39 68 300 265 486 Cuts at: 3855 5322 6468 7393 1353 Size: 1467 1146 925 3423 Fragments arranged by size: 3423 1467 1146 925 669 486 437 300 265 238 68 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6553 8050 8424 8570 387 Size: 1064 138 4964 1497 374 146 1280 Fragments arranged by size: 4964 1497 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5152 5152 Size: 9463 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2204 2367 2498 2529 2785 2882 Size: 393 422 150 163 131 31 256 97 Cuts at: 2882 3395 3871 4622 4733 4759 5053 5138 5463 Size: 513 476 751 111 26 294 85 325 Cuts at: 5463 5673 5955 6181 6220 6332 7526 8123 8910 Size: 210 282 226 39 112 1194 597 787 Cuts at: 8910 9178 1239 Size: 268 1524 Fragments arranged by size: 1524 1194 787 751 597 513 476 422 393 325 294 282 268 256 226 210 163 150 131 112 111 97 85 39 31 26 EcoNI CCTnn'n_nnAGG Cuts at: 2219 2456 3889 5523 2219 Size: 237 1433 1634 6159 Fragments arranged by size: 6159 1634 1433 237 EcoO109I rG'GnC_Cy Cuts at: 2824 3585 3736 3737 4367 4379 2824 Size: 761 151 1 630 12 7908 Fragments arranged by size: 7908 761 630 151 12 1 EcoRI G'AATT_C Cuts at: 4885 6772 7150 4885 Size: 1887 378 7198 Fragments arranged by size: 7198 1887 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2417 2518 2596 3425 4557 2417 Size: 101 78 829 1132 7323 Fragments arranged by size: 7323 1132 829 101 78 FseI GG_CCGG'CC Cuts at: 6528 6528 Size: 9463 FspI TGC'GCA Cuts at: 7969 7969 Size: 9463 HaeII r_GCGC'y Cuts at: 1678 2104 2908 3652 8610 1678 Size: 426 804 744 4958 2531 Fragments arranged by size: 4958 2531 804 744 426 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 3239 3271 3866 Size: 32 135 32 1076 32 559 32 595 Cuts at: 3866 3898 4304 4336 4547 4579 5459 5491 5983 Size: 32 406 32 211 32 880 32 492 Cuts at: 5983 6015 8104 8136 8178 8210 1373 Size: 32 2089 32 42 32 2626 Fragments arranged by size: 2626 2089 1076 880 595 559 492 406 211 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3502 6807 7053 669 Size: 988 281 1564 3305 246 3079 Fragments arranged by size: 3305 3079 1564 988 281 246 HindIII A'AGCT_T Cuts at: 748 2300 2601 4417 748 Size: 1552 301 1816 5794 Fragments arranged by size: 5794 1816 1552 301 HpaI GTT'AAC Cuts at: 7053 7053 Size: 9463 KasI G'GCGC_C Cuts at: 3648 3648 Size: 9463 KpnI G_GTAC'C Cuts at: 6788 6788 Size: 9463 MfeI C'AATT_G Cuts at: 7062 7062 Size: 9463 MluI A'CGCG_T Cuts at: 9339 9339 Size: 9463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2965 3074 3256 3471 3816 5139 5157 5613 Size: 1660 109 182 215 345 1323 18 456 Cuts at: 5613 6316 6328 8577 8761 1305 Size: 703 12 2249 184 2007 Fragments arranged by size: 2249 2007 1660 1323 703 456 345 215 184 182 109 18 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 5105 5960 6223 10 Size: 54 1425 343 3273 855 263 3250 Fragments arranged by size: 3273 3250 1425 855 343 263 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2627 4787 5803 7421 7780 7939 Size: 648 1014 447 2160 1016 1618 359 159 Cuts at: 7939 518 Size: 2042 Fragments arranged by size: 2160 2042 1618 1016 1014 648 447 359 159 MspA1I CmG'CkG Cuts at: 1606 2141 2793 3816 5483 6027 6183 6253 6520 Size: 535 652 1023 1667 544 156 70 267 Cuts at: 6520 6558 6584 6879 7556 8704 8949 1606 Size: 38 26 295 677 1148 245 2120 Fragments arranged by size: 2120 1667 1148 1023 677 652 544 535 295 267 245 156 70 38 26 NaeI GCC'GGC Cuts at: 6526 6526 Size: 9463 NarI GG'CG_CC Cuts at: 3649 3649 Size: 9463 NcoI C'CATG_G Cuts at: 513 1065 2022 3846 4803 6108 513 Size: 552 957 1824 957 1305 3868 Fragments arranged by size: 3868 1824 1305 957 957 552 NdeI CA'TA_TG Cuts at: 387 3208 387 Size: 2821 6642 NgoMIV G'CCGG_C Cuts at: 6524 6524 Size: 9463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9463 NotI GC'GGCC_GC Cuts at: 6910 6910 Size: 9463 NspI r_CATG'y Cuts at: 1132 1229 4693 6051 6449 6821 8366 1132 Size: 97 3464 1358 398 372 1545 2229 Fragments arranged by size: 3464 2229 1545 1358 398 372 97 PasI CC'CwG_GG Cuts at: 1348 5588 1348 Size: 4240 5223 PciI A'CATG_T Cuts at: 8362 8362 Size: 9463 PflMI CCAn_nnn'nTGG Cuts at: 1108 2761 6298 6511 1108 Size: 1653 3537 213 4060 Fragments arranged by size: 4060 3537 1653 213 PfoI T'CCnGG_A Cuts at: 2220 2898 2961 3996 5293 2220 Size: 678 63 1035 1297 6390 Fragments arranged by size: 6390 1297 1035 678 63 PmeI GTTT'AAAC Cuts at: 6767 6767 Size: 9463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4171 4203 7532 7564 9045 9077 4171 Size: 32 3329 32 1481 32 4557 Fragments arranged by size: 4557 3329 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2824 2824 Size: 9463 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9463 PsiI TTA'TAA Cuts at: 7033 7033 Size: 9463 PspOMI G'GGCC_C Cuts at: 2147 3736 4379 2147 Size: 1589 643 7231 Fragments arranged by size: 7231 1589 643 PspXI vC'TCGA_Gb Cuts at: 3835 3835 Size: 9463 PstI C_TGCA'G Cuts at: 830 2140 2880 2888 3565 3593 3674 4402 4686 Size: 1310 740 8 677 28 81 728 284 Cuts at: 4686 6815 830 Size: 2129 3478 Fragments arranged by size: 3478 2129 1310 740 728 677 284 81 28 8 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9463 PvuII CAG'CTG Cuts at: 2141 5483 6027 6183 6253 6520 2141 Size: 3342 544 156 70 267 5084 Fragments arranged by size: 5084 3342 544 267 156 70 SacI G_AGCT'C Cuts at: 5154 5154 Size: 9463 SacII CC_GC'GG Cuts at: 3817 3817 Size: 9463 SalI G'TCGA_C Cuts at: 1936 6805 1936 Size: 4869 4594 SapI GCTCTTCn'nnn_ Cuts at: 2022 2697 5322 2022 Size: 675 2625 6163 Fragments arranged by size: 6163 2625 675 SbfI CC_TGCA'GG Cuts at: 6815 6815 Size: 9463 ScaI AGT'ACT Cuts at: 2684 6230 7711 9423 2684 Size: 3546 1481 1712 2724 Fragments arranged by size: 3546 2724 1712 1481 SexAI A'CCwGG_T Cuts at: 6510 6510 Size: 9463 SfcI C'TryA_G Cuts at: 826 1045 2136 2876 2884 2913 3182 3487 3561 Size: 219 1091 740 8 29 269 305 74 Cuts at: 3561 3589 3670 3820 4141 4398 4547 4682 6550 Size: 28 81 150 321 257 149 135 1868 Cuts at: 6550 6811 7946 8627 8818 826 Size: 261 1135 681 191 1471 Fragments arranged by size: 1868 1471 1135 1091 740 681 321 305 269 261 257 219 191 150 149 135 81 74 29 28 8 SfiI GGCCn_nnn'nGGCC Cuts at: 6707 6707 Size: 9463 SfoI GGC'GCC Cuts at: 3650 3650 Size: 9463 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9463 SmaI CCC'GGG Cuts at: 1872 2108 2123 5433 6790 1872 Size: 236 15 3310 1357 4545 Fragments arranged by size: 4545 3310 1357 236 15 SmlI C'TyrA_G Cuts at: 820 1017 1943 2570 3200 3835 4830 5001 5133 Size: 197 926 627 630 635 995 171 132 Cuts at: 5133 5565 5607 7567 8468 8730 820 Size: 432 42 1960 901 262 1553 Fragments arranged by size: 1960 1553 995 926 901 635 630 627 432 262 197 171 132 42 SnaBI TAC'GTA Cuts at: 493 6760 7254 493 Size: 6267 494 2702 Fragments arranged by size: 6267 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9463 SphI G_CATG'C Cuts at: 1132 6821 1132 Size: 5689 3774 SrfI GCCC'GGGC Cuts at: 2123 2123 Size: 9463 SspI AAT'ATT Cuts at: 5 52 7387 5 Size: 47 7335 2081 Fragments arranged by size: 7335 2081 47 StuI AGG'CCT Cuts at: 1517 3924 4724 4978 6166 1517 Size: 2407 800 254 1188 4814 Fragments arranged by size: 4814 2407 1188 800 254 StyI C'CwwG_G Cuts at: 513 1065 2022 2820 3846 4651 4719 4725 4803 Size: 552 957 798 1026 805 68 6 78 Cuts at: 4803 5744 6108 6902 513 Size: 941 364 794 3074 Fragments arranged by size: 3074 1026 957 941 805 798 794 552 364 78 68 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2471 4308 4568 5546 7490 7507 7660 Size: 1255 891 1837 260 978 1944 17 153 Cuts at: 7660 7845 8290 325 Size: 185 445 1498 Fragments arranged by size: 1944 1837 1498 1255 978 891 445 260 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2682 2709 4196 Size: 274 80 33 51 1443 705 27 1487 Cuts at: 4196 5201 6228 6606 7709 9421 96 Size: 1005 1027 378 1103 1712 138 Fragments arranged by size: 1712 1487 1443 1103 1027 1005 705 378 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5136 5168 5667 5699 6132 6164 9423 9455 5136 Size: 32 499 32 433 32 3259 32 5144 Fragments arranged by size: 5144 3259 499 433 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9463 XbaI T'CTAG_A Cuts at: 6799 6799 Size: 9463 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2827 3721 3853 4270 5953 6010 2029 Size: 798 894 132 417 1683 57 5482 Fragments arranged by size: 5482 1683 894 798 417 132 57 XhoI C'TCGA_G Cuts at: 1943 3835 4830 1943 Size: 1892 995 6576 Fragments arranged by size: 6576 1892 995 XmaI C'CCGG_G Cuts at: 1870 2106 2121 5431 6788 1870 Size: 236 15 3310 1357 4545 Fragments arranged by size: 4545 3310 1357 236 15 XmnI GAAnn'nnTTC Cuts at: 7592 7592 Size: 9463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3418 7271 276 Size: 53 83 186 936 1884 3853 2468 Fragments arranged by size: 3853 2468 1884 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PmlI PsrI RsrII SanDI SwaI