(Circular) (Six-base) MAPSORT of: pFN21ASDA0965.seq Check: 8776 from: 1 to: 6121 pFN21ASDA0965 6121 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2404 2404 Size: 6121 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 3464 1937 Size: 1243 284 4594 Fragments arranged by size: 4594 1243 284 Acc65I G'GTAC_C Cuts at: 3064 3442 3064 Size: 378 5743 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4184 5568 5836 1632 Size: 2552 1384 268 1917 Fragments arranged by size: 2552 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 5020 5997 1203 Size: 3817 977 1327 Fragments arranged by size: 3817 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 2293 2941 4850 2293 Size: 648 1909 3564 Fragments arranged by size: 3564 1909 648 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6121 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 2579 Size: 32 6089 AlwNI CAG_nnn'CTG Cuts at: 2612 3864 5436 2612 Size: 1252 1572 3297 Fragments arranged by size: 3297 1572 1252 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 2110 2525 2756 3250 3430 3656 3808 5917 Size: 617 415 231 494 180 226 152 2109 Cuts at: 5917 1493 Size: 1697 Fragments arranged by size: 2109 1697 617 494 415 231 226 180 152 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 2073 3446 1870 Size: 73 130 1373 4545 Fragments arranged by size: 4545 1373 130 73 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2462 3064 3442 4897 618 Size: 325 217 627 675 602 378 1455 1842 Fragments arranged by size: 1842 1455 675 627 602 378 325 217 BanII G_rGCy'C Cuts at: 1391 3440 1391 Size: 2049 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4732 136 Size: 107 122 71 631 605 3060 1525 Fragments arranged by size: 3060 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6116 6116 Size: 6121 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 4182 5338 1790 Size: 2392 1156 2573 Fragments arranged by size: 2573 2392 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3193 3337 3852 Size: 416 53 83 399 24 1776 144 515 Cuts at: 3852 4810 442 Size: 958 1753 Fragments arranged by size: 1776 1753 958 515 416 399 144 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2556 2880 3042 4781 1239 Size: 1317 324 162 1739 2579 Fragments arranged by size: 2579 1739 1317 324 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2991 4246 5111 5409 2991 Size: 1255 865 298 3703 Fragments arranged by size: 3703 1255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4784 882 Size: 617 3285 2219 Fragments arranged by size: 3285 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3418 3912 493 Size: 711 2214 494 2702 Fragments arranged by size: 2702 2214 711 494 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3928 4310 4945 275 Size: 53 83 186 936 2395 382 635 1451 Fragments arranged by size: 2395 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3307 4554 4952 5226 5373 5784 3307 Size: 1247 398 274 147 411 3644 Fragments arranged by size: 3644 1247 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5896 Fragments arranged by size: 5896 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2331 1186 Size: 1145 4976 BseYI C'CCAG_C Cuts at: 3404 5324 5820 3404 Size: 1920 496 3705 Fragments arranged by size: 3705 1920 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5548 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3571 4332 5360 1540 Size: 275 186 1570 761 1028 2301 Fragments arranged by size: 2301 1570 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3192 4182 4267 5338 3192 Size: 990 85 1071 3975 Fragments arranged by size: 3975 1071 990 85 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3192 3440 4182 4267 5338 1391 Size: 399 1402 248 742 85 1071 2174 Fragments arranged by size: 2174 1402 1071 742 399 248 85 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2404 3476 844 Size: 1560 1072 3489 Fragments arranged by size: 3489 1560 1072 BsrBI CCG'CTC Cuts at: 1394 1823 4008 1394 Size: 429 2185 3507 Fragments arranged by size: 3507 2185 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2034 2379 2552 2889 4616 4790 65 Size: 1183 786 345 173 337 1727 174 1396 Fragments arranged by size: 1727 1396 1183 786 345 337 174 173 BsrFI r'CCGG_y Cuts at: 1700 1810 4765 4952 1700 Size: 110 2955 187 2869 Fragments arranged by size: 2955 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 2462 2627 794 Size: 1668 165 4288 Fragments arranged by size: 4288 1668 165 BstBI TT'CG_AA Cuts at: 2007 4925 2007 Size: 2918 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6121 BstYI r'GATC_y Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116 Size: 1479 367 385 17 1452 86 12 346 Cuts at: 6116 1972 Size: 1977 Fragments arranged by size: 1977 1479 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2178 513 Size: 552 957 156 4456 Fragments arranged by size: 4456 957 552 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 3637 4431 4451 3637 Size: 794 20 5307 Fragments arranged by size: 5307 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DraIII CAC_nnn'GTG Cuts at: 2789 2789 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 3568 4457 Size: 54 1425 50 275 18 345 1393 889 Cuts at: 4457 6086 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1393 889 345 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2199 2308 2577 4051 1353 Size: 783 63 109 269 1474 3423 Fragments arranged by size: 3423 1474 783 269 109 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2556 2880 3042 4184 4781 5568 5836 Size: 393 924 324 162 1142 597 787 268 Cuts at: 5836 1239 Size: 1524 Fragments arranged by size: 1524 1142 924 787 597 393 324 268 162 EcoNI CCTnn'n_nnAGG Cuts at: 3352 3352 Size: 6121 EcoRI G'AATT_C Cuts at: 3430 3808 3430 Size: 378 5743 EcoRV GAT'ATC Cuts at: 2981 2981 Size: 6121 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2337 2337 Size: 6121 FspI TGC'GCA Cuts at: 4627 4627 Size: 6121 HaeII r_GCGC'y Cuts at: 1678 5268 1678 Size: 3590 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2279 2311 2927 2959 3302 Size: 32 135 32 707 32 616 32 343 Cuts at: 3302 3334 4762 4794 4836 4868 1373 Size: 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 707 616 343 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3181 3369 3465 3711 669 Size: 988 281 1243 188 96 246 3079 Fragments arranged by size: 3079 1243 988 281 246 188 96 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 3068 3446 3068 Size: 378 5743 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5235 5419 1305 Size: 3930 184 2007 Fragments arranged by size: 3930 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 10 Size: 54 1425 343 345 3954 Fragments arranged by size: 3954 1425 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2367 3395 4079 4438 4597 518 Size: 648 861 340 1028 684 359 159 2042 Fragments arranged by size: 2042 1028 861 684 648 359 340 159 MspA1I CmG'CkG Cuts at: 1606 3537 4214 5362 5607 1606 Size: 1931 677 1148 245 2120 Fragments arranged by size: 2120 1931 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2178 513 Size: 552 957 156 4456 Fragments arranged by size: 4456 957 552 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 3479 5024 1132 Size: 97 2250 1545 2229 Fragments arranged by size: 2250 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 2641 3327 1108 Size: 1533 686 3902 Fragments arranged by size: 3902 1533 686 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2798 2830 4190 4222 5703 5735 2798 Size: 32 1360 32 1481 32 3184 Fragments arranged by size: 3184 1481 1360 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PstI C_TGCA'G Cuts at: 830 3473 830 Size: 2643 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3179 3463 1936 Size: 1243 284 4594 Fragments arranged by size: 4594 1243 284 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2088 3219 3469 4604 5285 5476 826 Size: 219 1043 1131 250 1135 681 191 1471 Fragments arranged by size: 1471 1135 1131 1043 681 250 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 2075 3448 1872 Size: 203 1373 4545 Fragments arranged by size: 4545 1373 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 3006 4225 5126 5388 820 Size: 197 926 1063 1219 901 262 1553 Fragments arranged by size: 1553 1219 1063 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3418 3912 493 Size: 2925 494 2702 Fragments arranged by size: 2925 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3479 1132 Size: 2347 3774 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6121 StyI C'CwwG_G Cuts at: 513 1065 2022 2178 2691 3084 3560 513 Size: 552 957 156 513 393 476 3074 Fragments arranged by size: 3074 957 552 513 476 393 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2458 4148 4165 4318 4503 4948 325 Size: 1255 878 1690 17 153 185 445 1498 Fragments arranged by size: 1690 1498 1255 878 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4367 6079 96 Size: 274 80 33 51 1443 2390 1712 138 Fragments arranged by size: 2390 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6081 6113 6081 Size: 32 6089 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2101 2278 2101 Size: 177 5944 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 2073 3446 1870 Size: 203 1373 4545 Fragments arranged by size: 4545 1373 203 XmnI GAAnn'nnTTC Cuts at: 4250 4250 Size: 6121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI