(Circular) (Six-base) MAPSORT of: pFN21ASDA0962.seq Check: 9577 from: 1 to: 7075 pFN21ASDA0962 7075 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4885 278 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3288 3957 4418 1937 Size: 1351 669 461 4594 Fragments arranged by size: 4594 1351 669 461 Acc65I G'GTAC_C Cuts at: 4396 4396 Size: 7075 AclI AA'CG_TT Cuts at: 1563 5202 5575 1563 Size: 3639 373 3063 Fragments arranged by size: 3639 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3525 5138 6522 6790 1632 Size: 1893 1613 1384 268 1917 Fragments arranged by size: 1917 1893 1613 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6878 AflIII A'CryG_T Cuts at: 1203 5974 6951 1203 Size: 4771 977 1327 Fragments arranged by size: 4771 1327 977 AgeI A'CCGG_T Cuts at: 5906 5906 Size: 7075 AhdI GACnn_n'nnGTC Cuts at: 5804 5804 Size: 7075 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7075 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3327 1184 Size: 2143 4932 AlwNI CAG_nnn'CTG Cuts at: 4818 6390 4818 Size: 1572 5503 ApaLI G'TGCA_C Cuts at: 5132 6288 5132 Size: 1156 5919 ApoI r'AATT_y Cuts at: 1493 2085 2374 3446 3913 3941 4040 4384 4610 Size: 592 289 1072 467 28 99 344 226 Cuts at: 4610 4762 6871 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1072 592 467 344 289 226 152 99 28 AseI AT'TA_AT Cuts at: 160 1032 5629 160 Size: 872 4597 1606 Fragments arranged by size: 4597 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 AvaI C'yCGr_G Cuts at: 1870 1943 2181 3048 4236 4400 1870 Size: 73 238 867 1188 164 4545 Fragments arranged by size: 4545 1188 867 238 164 73 AvrII C'CTAG_G Cuts at: 2122 2650 2677 2122 Size: 528 27 6520 Fragments arranged by size: 6520 528 27 BamHI G'GATC_C Cuts at: 4262 4405 4772 4262 Size: 143 367 6565 Fragments arranged by size: 6565 367 143 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4396 5851 618 Size: 325 217 627 2609 1455 1842 Fragments arranged by size: 2609 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4328 4394 1391 Size: 2937 66 4072 Fragments arranged by size: 4072 2937 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2229 2747 3423 3764 928 Size: 971 330 518 676 341 4239 Fragments arranged by size: 4239 971 676 518 341 330 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2533 2567 3518 3552 3667 3701 5266 Size: 34 596 34 951 34 115 34 1565 Cuts at: 5266 5300 1903 Size: 34 3678 Fragments arranged by size: 3678 1565 951 596 115 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4967 6177 4967 Size: 1210 5865 BclI T'GATC_A Cuts at: 1548 2290 1548 Size: 742 6333 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 5686 136 Size: 107 122 71 631 605 475 3539 1525 Fragments arranged by size: 3539 1525 631 605 475 122 107 71 BglII A'GATC_T Cuts at: 7070 7070 Size: 7075 BlpI GC'TnA_GC Cuts at: 4492 4492 Size: 7075 Bme1580I G_kGCm'C Cuts at: 1790 2659 5136 6292 1790 Size: 869 2477 1156 2573 Fragments arranged by size: 2573 2477 1156 869 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7075 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3771 3978 4806 Size: 416 53 83 399 24 2354 207 828 Cuts at: 4806 5764 442 Size: 958 1753 Fragments arranged by size: 2354 1753 958 828 416 399 207 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7075 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2235 2328 3640 3717 4340 5735 1239 Size: 996 93 1312 77 623 1395 2579 Fragments arranged by size: 2579 1395 1312 996 623 93 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3246 4032 5200 6065 6363 3246 Size: 786 1168 865 298 3958 Fragments arranged by size: 3958 1168 865 786 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5738 882 Size: 617 4239 2219 Fragments arranged by size: 4239 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4372 4866 493 Size: 711 3168 494 2702 Fragments arranged by size: 3168 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7069 7069 Size: 7075 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2635 4882 5264 5899 Size: 53 83 186 936 1102 2247 382 635 Cuts at: 5899 275 Size: 1451 Fragments arranged by size: 2247 1451 1102 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5508 5906 6180 6327 6738 5508 Size: 398 274 147 411 5845 Fragments arranged by size: 5845 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3424 3454 1178 Size: 30 165 30 2021 30 4799 Fragments arranged by size: 4799 2021 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4193 1186 Size: 3007 4068 BseYI C'CCAG_C Cuts at: 2621 3594 6278 6774 2621 Size: 973 2684 496 2922 Fragments arranged by size: 2922 2684 973 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4219 1163 Size: 573 2483 4019 Fragments arranged by size: 4019 2483 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3161 4525 5286 6314 1540 Size: 275 186 1160 1364 761 1028 2301 Fragments arranged by size: 2301 1364 1160 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5136 5221 6292 5136 Size: 85 1071 5919 Fragments arranged by size: 5919 1071 85 BsmI GAATG_Cn' Cuts at: 2544 4586 4679 2544 Size: 2042 93 4940 Fragments arranged by size: 4940 2042 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2295 2626 2295 Size: 331 6744 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2659 4328 4394 5136 5221 6292 1391 Size: 399 869 1669 66 742 85 1071 2174 Fragments arranged by size: 2174 1669 1071 869 742 399 85 66 BspEI T'CCGG_A Cuts at: 6738 6738 Size: 7075 BspHI T'CATG_A Cuts at: 4964 6694 4964 Size: 1730 5345 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4430 844 Size: 3586 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4962 1394 Size: 429 3139 3507 Fragments arranged by size: 3507 3139 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5570 5744 65 Size: 1183 4322 174 1396 Fragments arranged by size: 4322 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5719 5906 1700 Size: 110 3909 187 2869 Fragments arranged by size: 3909 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7075 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7075 BssSI C'ACGA_G Cuts at: 5135 6147 5135 Size: 1012 6063 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7075 BstBI TT'CG_AA Cuts at: 2007 5879 2007 Size: 3872 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7075 BstXI CCAn_nnnn'nTGG Cuts at: 3865 3865 Size: 7075 BstYI r'GATC_y Cuts at: 1972 2209 3484 4262 4405 4772 5157 5174 6626 Size: 237 1275 778 143 367 385 17 1452 Cuts at: 6626 6712 6724 7070 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1275 778 385 367 346 237 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5566 Fragments arranged by size: 5566 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6336 Fragments arranged by size: 6336 683 56 BtsI GCAGTG_nn' Cuts at: 3516 4207 4591 5385 5405 3516 Size: 691 384 794 20 5186 Fragments arranged by size: 5186 794 691 384 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7075 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7040 DraI TTT'AAA Cuts at: 3010 4379 3010 Size: 1369 5706 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6082 809 Size: 507 4766 1802 Fragments arranged by size: 4766 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4129 4522 5411 Size: 54 1425 50 275 18 2299 393 889 Cuts at: 5411 7040 8 Size: 1629 43 Fragments arranged by size: 2299 1629 1425 889 393 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4522 1537 Size: 275 2710 4090 Fragments arranged by size: 4090 2710 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2949 3498 3668 3677 4157 4170 4219 5005 Size: 1596 549 170 9 480 13 49 786 Cuts at: 5005 1353 Size: 3423 Fragments arranged by size: 3423 1596 786 549 480 170 49 13 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2348 5662 6036 6182 387 Size: 1064 138 759 3314 374 146 1280 Fragments arranged by size: 3314 1280 1064 759 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2328 3525 3640 3717 4340 5138 Size: 393 603 93 1197 115 77 623 798 Cuts at: 5138 5735 6522 6790 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1197 798 787 623 603 597 393 268 115 93 77 EcoNI CCTnn'n_nnAGG Cuts at: 3813 4319 4333 3813 Size: 506 14 6555 Fragments arranged by size: 6555 506 14 EcoRI G'AATT_C Cuts at: 3913 4384 4762 3913 Size: 471 378 6226 Fragments arranged by size: 6226 471 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3530 4159 3530 Size: 629 6446 FspI TGC'GCA Cuts at: 5581 5581 Size: 7075 HaeII r_GCGC'y Cuts at: 1678 6222 1678 Size: 4544 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2900 2932 3569 3601 3707 Size: 32 135 32 1328 32 637 32 106 Cuts at: 3707 3739 4196 4228 5716 5748 5790 5822 1373 Size: 32 457 32 1488 32 42 32 2626 Fragments arranged by size: 2626 1488 1328 637 457 135 106 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 4210 4419 4665 669 Size: 988 281 951 1321 209 246 3079 Fragments arranged by size: 3079 1321 988 951 281 246 209 HindIII A'AGCT_T Cuts at: 748 3778 748 Size: 3030 4045 HpaI GTT'AAC Cuts at: 4665 4665 Size: 7075 KpnI G_GTAC'C Cuts at: 4400 4400 Size: 7075 MfeI C'AATT_G Cuts at: 2433 4674 2433 Size: 2241 4834 MluI A'CGCG_T Cuts at: 6951 6951 Size: 7075 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 6189 6373 1305 Size: 774 4110 184 2007 Fragments arranged by size: 4110 2007 774 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4131 10 Size: 54 1425 343 2299 2954 Fragments arranged by size: 2954 2299 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2367 3891 3893 5033 5392 5551 Size: 648 861 340 1524 2 1140 359 159 Cuts at: 5551 518 Size: 2042 Fragments arranged by size: 2042 1524 1140 861 648 359 340 159 2 MspA1I CmG'CkG Cuts at: 1606 3359 3475 3978 4491 5168 6316 6561 1606 Size: 1753 116 503 513 677 1148 245 2120 Fragments arranged by size: 2120 1753 1148 677 513 503 245 116 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5566 Fragments arranged by size: 5566 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7075 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7075 NotI GC'GGCC_GC Cuts at: 4522 4522 Size: 7075 NspI r_CATG'y Cuts at: 1132 1229 3287 4433 5978 1132 Size: 97 2058 1146 1545 2229 Fragments arranged by size: 2229 2058 1545 1146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7075 PciI A'CATG_T Cuts at: 5974 5974 Size: 7075 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7075 PfoI T'CCnGG_A Cuts at: 2212 2212 Size: 7075 PmeI GTTT'AAAC Cuts at: 4379 4379 Size: 7075 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5144 5176 6657 6689 5144 Size: 32 1481 32 5530 Fragments arranged by size: 5530 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7075 PsiI TTA'TAA Cuts at: 2169 4645 2169 Size: 2476 4599 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2306 2338 2306 Size: 32 7043 PstI C_TGCA'G Cuts at: 830 4427 830 Size: 3597 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7075 PvuII CAG'CTG Cuts at: 3475 3978 3475 Size: 503 6572 SalI G'TCGA_C Cuts at: 1936 4417 1936 Size: 2481 4594 SapI GCTCTTCn'nnn_ Cuts at: 3498 3498 Size: 7075 SbfI CC_TGCA'GG Cuts at: 4427 4427 Size: 7075 ScaI AGT'ACT Cuts at: 3179 5323 7035 3179 Size: 2144 1712 3219 Fragments arranged by size: 3219 2144 1712 SfcI C'TryA_G Cuts at: 826 1045 3289 3935 4423 5558 6239 6430 826 Size: 219 2244 646 488 1135 681 191 1471 Fragments arranged by size: 2244 1471 1135 681 646 488 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7075 SmaI CCC'GGG Cuts at: 1872 2183 4402 1872 Size: 311 2219 4545 Fragments arranged by size: 4545 2219 311 SmlI C'TyrA_G Cuts at: 820 1017 1943 3261 4047 4236 5179 6080 6342 Size: 197 926 1318 786 189 943 901 262 Cuts at: 6342 820 Size: 1553 Fragments arranged by size: 1553 1318 943 926 901 786 262 197 189 SnaBI TAC'GTA Cuts at: 493 4372 4866 493 Size: 3879 494 2702 Fragments arranged by size: 3879 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7075 SphI G_CATG'C Cuts at: 1132 4433 1132 Size: 3301 3774 SspI AAT'ATT Cuts at: 5 52 4999 5 Size: 47 4947 2081 Fragments arranged by size: 4947 2081 47 StuI AGG'CCT Cuts at: 1517 3028 1517 Size: 1511 5564 StyI C'CwwG_G Cuts at: 513 1065 2022 2122 2650 2677 3422 4258 4514 Size: 552 957 100 528 27 745 836 256 Cuts at: 4514 513 Size: 3074 Fragments arranged by size: 3074 957 836 745 552 528 256 100 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2585 3542 4217 5102 5119 5272 5457 Size: 1255 1005 957 675 885 17 153 185 Cuts at: 5457 5902 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1005 957 885 675 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3177 4114 5321 Size: 274 80 33 51 1443 1200 937 1207 Cuts at: 5321 7033 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1207 1200 937 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2595 2627 3166 3198 3384 3416 3418 3424 3450 Size: 32 539 32 186 32 2 6 26 Cuts at: 3450 3456 7035 7067 2595 Size: 6 3579 32 2603 Fragments arranged by size: 3579 2603 539 186 32 32 32 32 26 6 6 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7075 XbaI T'CTAG_A Cuts at: 4003 4411 4003 Size: 408 6667 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3758 3758 Size: 7075 XhoI C'TCGA_G Cuts at: 1943 4236 1943 Size: 2293 4782 XmaI C'CCGG_G Cuts at: 1870 2181 4400 1870 Size: 311 2219 4545 Fragments arranged by size: 4545 2219 311 XmnI GAAnn'nnTTC Cuts at: 2481 2574 2754 3131 4142 5204 2481 Size: 93 180 377 1011 1062 4352 Fragments arranged by size: 4352 1062 1011 377 180 93 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4883 276 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI