Restriction map for
pFN21ASDA0958
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BbvCI
BcgI BfrI BglI BglII BlnI
BsaBI BsgI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI DraI
DraIII DrdI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI KspI MamI MfeI
MluI MseI MslI MspA1I MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AatII 145 GACGTC GACGT^C
AccII 846 CGCG CG^CG
AccIII 299 TCCGGA T^CCGGA
AhdI 552 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 552 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1078 GAGCTC G[AT]GC[AT]^C
AvaI 1039 CCCGAG C^[CT]CG[GA]G
BalI 989 TGGCCA TGG^CCA
BamHI 797 GGATCC G^GATCC
BbeI 606 GGCGCC GGCGC^C
BbrPI 1062 CACGTG CAC^GTG
BciVI 702 GGATAC GTATCC(6/5)
BclI 486 TGATCA T^GATCA
BfaI 850 CTAG C^TAG
BlpI 590 GCTCAGC GC^T[ACGT]AGC
BpmI 1075 CTGGAG CTGGAG(16/14)
Bpu1102I 590 GCTCAGC GC^T[ACGT]AGC
BsaAI 1062 CACGTG [CT]AC^GT[GA]
BsaI 550 GAGACC GGTCTC(1/5)
BsaWI 299 TCCGGA [AT]^CCGG[AT]
BseAI 299 TCCGGA T^CCGGA
BsiHKAI 1078 GAGCTC G[AT]GC[AT]^C
BsoBI 1039 CCCGAG C^[CT]CG[GA]G
BspEI 299 TCCGGA T^CCGGA
BspHI 956 TCATGA T^CATGA
BsrFI 968 ACCGGC [GA]^CCGG[CT]
BssSI 514 CACGAG CACGAG(-5/-1)
BstUI 846 CGCG CG^CG
Cac8I 1139 GCCTGC GC[ACGT]^[ACGT]GC
CelII 590 GCTCAGC GC^T[ACGT]AGC
Cfr10I 968 ACCGGC [GA]^CCGG[CT]
CpoI 637 CGGTCCG CG^G[AT]CCG
Eam1105I 552 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 735 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 253 CCATGG C^C[AT][AT]GG
FbaI 486 TGATCA T^GATCA
HaeII 606 GGCGCC [GA]GCGC^[CT]
HgaI 787 GACGC GACGC(5/10)
HincII 148 GTCAAC GT[CT]^[GA]AC
HindII 148 GTCAAC GT[CT]^[GA]AC
HphI 1047 GGTGA GGTGA(8/7)
KasI 606 GGCGCC G^GCGCC
MaeI 850 CTAG C^TAG
MluNI 989 TGGCCA TGG^CCA
MroI 299 TCCGGA T^CCGGA
MscI 989 TGGCCA TGG^CCA
MvnI 846 CGCG CG^CG
NarI 606 GGCGCC GG^CGCC
NcoI 253 CCATGG C^CATGG
PflMI 966 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1062 CACGTG CAC^GTG
PmlI 1062 CACGTG CAC^GTG
PpuMI 448 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 956 TCATGA T^CATGA
RsrII 637 CGGTCCG CG^G[AT]CCG
SacI 1078 GAGCTC GAGCT^C
SapI 1018 GAAGAGC GCTCTTC(1/4)
SfaNI 997 GATGC GCATC(5/9)
SfcI 522 CTACAG C^T[GA][CT]AG
SfoI 606 GGCGCC GGC^GCC
StyI 253 CCATGG C^C[AT][AT]GG
TaqI 359 TCGA T^CGA
TthHB8I 359 TCGA T^CGA
Van91I 966 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
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AcyI 145 GACGTC G[GA]^CG[CT]C
606 GGCGCC
AlwNI 380 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
490 CAGCTCCTG
ApaI 391 GGGCCC GGGCC^C
855 GGGCCC
AviII 173 TGCGCA TGC^GCA
1123 TGCGCA
BsaHI 145 GACGTC G[GA]^CG[CT]C
606 GGCGCC
BsmBI 567 CGTCTC CGTCTC(1/5)
749 GAGACG
BsmFI 448 GGGAC GGGAC(10/14)
1196 GTCCC
BspMI 170 ACCTGC ACCTGC(4/8)
734 ACCTGC
BsrBI 472 GAGCGG CCGCTC(-3/-3)
1164 GAGCGG
DsaI 253 CCATGG C^C[GA][CT]GG
779 CCACGG
EarI 457 GAAGAG CTCTTC(1/4)
1018 GAAGAG
FspI 173 TGCGCA TGC^GCA
1123 TGCGCA
Hin1I 145 GACGTC G[GA]^CG[CT]C
606 GGCGCC
Ksp632I 457 GAAGAG CTCTTC(1/4)
1018 GAAGAG
ScaI 518 AGTACT AGT^ACT
1202 AGTACT
SexAI 293 ACCAGGT A^CC[AT]GGT
404 ACCAGGT
Tsp45I 902 GTCAC ^GT[GC]AC
1149 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpuAI BsaJI BseRI BsiEI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI CfoI
Cfr13I DdeI DpnII DraII EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TfiI TseI
TspRI XhoII