(Circular) (Six-base) MAPSORT of: pFN21ASDA0958.seq Check: 6181 from: 1 to: 6001 pFN21ASDA0958 6001 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2766 2766 Size: 6001 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2172 3811 278 Size: 53 83 186 936 636 1639 2468 Fragments arranged by size: 2468 1639 936 636 186 83 53 AccI GT'mk_AC Cuts at: 1937 3294 3344 1937 Size: 1357 50 4594 Fragments arranged by size: 4594 1357 50 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 2264 4064 5448 5716 1632 Size: 620 12 1800 1384 268 1917 Fragments arranged by size: 1917 1800 1384 620 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 2580 4730 2580 Size: 2150 3851 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AlwNI CAG_nnn'CTG Cuts at: 2408 2518 3744 5316 2408 Size: 110 1226 1572 3093 Fragments arranged by size: 3093 1572 1226 110 ApaI G_GGCC'C Cuts at: 2418 2882 2418 Size: 464 5537 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 3310 3536 3688 5797 1493 Size: 1817 226 152 2109 1697 Fragments arranged by size: 2109 1817 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3062 3326 1870 Size: 73 1119 264 4545 Fragments arranged by size: 4545 1119 264 73 BamHI G'GATC_C Cuts at: 2820 3331 3698 2820 Size: 511 367 5123 Fragments arranged by size: 5123 511 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2629 2754 3159 3322 4777 Size: 325 217 627 842 125 405 163 1455 Cuts at: 4777 618 Size: 1842 Fragments arranged by size: 1842 1455 842 627 405 325 217 163 125 BanII G_rGCy'C Cuts at: 1391 2418 2615 2867 2882 3105 3320 1391 Size: 1027 197 252 15 223 215 4072 Fragments arranged by size: 4072 1027 252 223 215 197 15 BbeI G_GCGC'C Cuts at: 2633 2633 Size: 6001 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2035 2240 2252 2990 928 Size: 971 136 205 12 738 3939 Fragments arranged by size: 3939 971 738 205 136 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4192 4226 1903 Size: 34 2255 34 3678 Fragments arranged by size: 3678 2255 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2719 3893 5103 2719 Size: 1174 1210 3617 Fragments arranged by size: 3617 1210 1174 BclI T'GATC_A Cuts at: 1548 2509 1548 Size: 961 5040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4612 136 Size: 107 122 71 631 605 2940 1525 Fragments arranged by size: 2940 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 2614 3418 2614 Size: 804 5197 Bme1580I G_kGCm'C Cuts at: 1790 2418 2882 4062 5218 1790 Size: 628 464 1180 1156 2573 Fragments arranged by size: 2573 1180 1156 628 464 BmgBI CAC'GTC Cuts at: 1318 3171 1318 Size: 1853 4148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2731 2937 3732 Size: 416 53 83 399 24 1314 206 795 Cuts at: 3732 4690 442 Size: 958 1753 Fragments arranged by size: 1753 1314 958 795 416 399 206 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3119 4661 1239 Size: 1880 1542 2579 Fragments arranged by size: 2579 1880 1542 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4126 4991 5289 4126 Size: 865 298 4838 Fragments arranged by size: 4838 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2567 4664 882 Size: 617 1068 2097 2219 Fragments arranged by size: 2219 2097 1068 617 BsaAI yAC'GTr Cuts at: 493 1204 3087 3298 3792 493 Size: 711 1883 211 494 2702 Fragments arranged by size: 2702 1883 711 494 211 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 2630 3808 4190 Size: 53 83 186 936 636 461 1178 382 Cuts at: 4190 4825 275 Size: 635 1451 Fragments arranged by size: 1451 1178 936 636 635 461 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2322 4434 4832 5106 5253 5664 2322 Size: 2112 398 274 147 411 2659 Fragments arranged by size: 2659 2112 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5776 Fragments arranged by size: 5776 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2585 3107 3278 1186 Size: 1081 318 522 171 3909 Fragments arranged by size: 3909 1081 522 318 171 BseYI C'CCAG_C Cuts at: 2053 2860 5204 5700 2053 Size: 807 2344 496 2354 Fragments arranged by size: 2354 2344 807 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5428 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 2501 3183 3451 4212 5240 Size: 275 186 120 380 682 268 761 1028 Cuts at: 5240 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 682 380 275 268 186 120 BsiHKAI G_wGCw'C Cuts at: 3105 4062 4147 5218 3105 Size: 957 85 1071 3888 Fragments arranged by size: 3888 1071 957 85 BsmI GAATG_Cn' Cuts at: 3512 3605 3512 Size: 93 5908 BsmBI CGTCTCn'nnnn_ Cuts at: 2596 2766 2596 Size: 170 5831 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2418 2615 2867 2882 3105 3320 4062 Size: 399 628 197 252 15 223 215 742 Cuts at: 4062 4147 5218 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 628 399 252 223 215 197 85 15 BspEI T'CCGG_A Cuts at: 2322 5664 2322 Size: 3342 2659 BspHI T'CATG_A Cuts at: 2979 3890 5620 2979 Size: 911 1730 3360 Fragments arranged by size: 3360 1730 911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2766 3356 844 Size: 1358 564 590 3489 Fragments arranged by size: 3489 1358 590 564 BsrBI CCG'CTC Cuts at: 1394 1823 2497 3189 3888 1394 Size: 429 674 692 699 3507 Fragments arranged by size: 3507 699 692 674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4496 4670 65 Size: 1183 3248 174 1396 Fragments arranged by size: 3248 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2991 4645 4832 1700 Size: 110 1181 1654 187 2869 Fragments arranged by size: 2869 1654 1181 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 2537 4061 5073 2537 Size: 1524 1012 3465 Fragments arranged by size: 3465 1524 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6001 BstBI TT'CG_AA Cuts at: 2007 4805 2007 Size: 2798 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstYI r'GATC_y Cuts at: 1972 2820 2967 3141 3331 3698 4083 4100 5552 Size: 848 147 174 190 367 385 17 1452 Cuts at: 5552 5638 5650 5996 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 848 385 367 346 190 174 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2276 2802 513 Size: 552 957 254 526 3712 Fragments arranged by size: 3712 957 552 526 254 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2383 487 Size: 683 56 1157 4105 Fragments arranged by size: 4105 1157 683 56 BtsI GCAGTG_nn' Cuts at: 2067 3517 4311 4331 2067 Size: 1450 794 20 3737 Fragments arranged by size: 3737 1450 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2118 2498 Size: 54 1425 50 275 18 259 29 380 Cuts at: 2498 3012 3180 3448 4337 5966 8 Size: 514 168 268 889 1629 43 Fragments arranged by size: 1629 1425 889 514 380 275 268 259 168 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2118 2498 3180 3448 1537 Size: 275 306 380 682 268 4090 Fragments arranged by size: 4090 682 380 306 275 268 EarI CTCTTCn'nnn_ Cuts at: 1353 2475 3036 3931 1353 Size: 1122 561 895 3423 Fragments arranged by size: 3423 1122 895 561 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4588 4962 5108 387 Size: 1064 138 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3103 3103 Size: 6001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2264 3119 4064 4661 5448 5716 Size: 393 620 12 855 945 597 787 268 Cuts at: 5716 1239 Size: 1524 Fragments arranged by size: 1524 945 855 787 620 597 393 268 12 EcoNI CCTnn'n_nnAGG Cuts at: 2762 2762 Size: 6001 EcoO109I rG'GnC_Cy Cuts at: 2415 2472 2878 2879 2415 Size: 57 406 1 5537 Fragments arranged by size: 5537 406 57 1 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FspI TGC'GCA Cuts at: 2198 3148 4507 2198 Size: 950 1359 3692 Fragments arranged by size: 3692 1359 950 HaeII r_GCGC'y Cuts at: 1678 2633 5148 1678 Size: 955 2515 2531 Fragments arranged by size: 2531 2515 955 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2566 2598 2776 Size: 32 135 32 542 32 420 32 178 Cuts at: 2776 2808 3176 3208 4642 4674 4716 4748 1373 Size: 32 368 32 1434 32 42 32 2626 Fragments arranged by size: 2626 1434 542 420 368 178 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2173 3345 3591 669 Size: 988 281 235 1172 246 3079 Fragments arranged by size: 3079 1172 988 281 246 235 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KasI G'GCGC_C Cuts at: 2629 2629 Size: 6001 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3014 10 Size: 54 1425 343 1182 2997 Fragments arranged by size: 2997 1425 1182 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3959 4318 4477 518 Size: 648 2793 359 159 2042 Fragments arranged by size: 2793 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3417 4094 5242 5487 1606 Size: 1811 677 1148 245 2120 Fragments arranged by size: 2120 1811 1148 677 245 NarI GG'CG_CC Cuts at: 2630 2630 Size: 6001 NcoI C'CATG_G Cuts at: 513 1065 2022 2276 513 Size: 552 957 254 4238 Fragments arranged by size: 4238 957 552 254 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 3359 4904 1132 Size: 97 2130 1545 2229 Fragments arranged by size: 2229 2130 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 2995 1108 Size: 1887 4114 PfoI T'CCnGG_A Cuts at: 2829 2829 Size: 6001 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PmlI CAC'GTG Cuts at: 3087 3087 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2472 2472 Size: 6001 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PspOMI G'GGCC_C Cuts at: 2414 2878 2414 Size: 464 5537 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2378 2410 2378 Size: 32 5969 PstI C_TGCA'G Cuts at: 830 3353 830 Size: 2523 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 RsrII CG'GwC_CG Cuts at: 2661 2661 Size: 6001 SacI G_AGCT'C Cuts at: 3105 3105 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SapI GCTCTTCn'nnn_ Cuts at: 3036 3036 Size: 6001 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 2543 3227 4249 5961 2543 Size: 684 1022 1712 2583 Fragments arranged by size: 2583 1712 1022 684 SexAI A'CCwGG_T Cuts at: 2316 2427 2316 Size: 111 5890 SfcI C'TryA_G Cuts at: 826 1045 2545 3349 4484 5165 5356 826 Size: 219 1500 804 1135 681 191 1471 Fragments arranged by size: 1500 1471 1135 804 681 219 191 SfoI GGC'GCC Cuts at: 2631 2631 Size: 6001 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4105 5006 5268 820 Size: 197 926 2162 901 262 1553 Fragments arranged by size: 2162 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3298 3792 493 Size: 2805 494 2702 Fragments arranged by size: 2805 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2022 2276 3440 513 Size: 552 957 254 1164 3074 Fragments arranged by size: 3074 1164 957 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2248 4028 4045 4198 4383 4828 325 Size: 1255 668 1780 17 153 185 445 1498 Fragments arranged by size: 1780 1498 1255 668 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 2541 3225 Size: 274 80 33 51 1443 543 21 684 Cuts at: 3225 4247 5959 96 Size: 1022 1712 138 Fragments arranged by size: 1712 1443 1022 684 543 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5961 5993 5961 Size: 32 5969 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 4130 4130 Size: 6001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2170 3809 276 Size: 53 83 186 936 636 1639 2468 Fragments arranged by size: 2468 1639 936 636 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PvuII SacII SanDI SfiI SrfI SwaI XcmI