Restriction map for
pFN21ASDA0958
(Linear) (Six-base) MAPSORT of: KIAA0958.seq Check: 559 from: 1 to: 1272
KIAA0958.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 743 1272
Size: 743 529
AatII G_ACGT'C
Cuts at: 0 149 1272
Size: 149 1123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 241 1272
Size: 229 12 1031
Fragments arranged by size:
1031 229 12
AhdI GACnn_n'nnGTC
Cuts at: 0 557 1272
Size: 557 715
AlwNI CAG_nnn'CTG
Cuts at: 0 385 495 1272
Size: 385 110 777
Fragments arranged by size:
777 385 110
ApaI G_GGCC'C
Cuts at: 0 395 859 1272
Size: 395 464 413
Fragments arranged by size:
464 413 395
AvaI C'yCGr_G
Cuts at: 0 1039 1272
Size: 1039 233
BamHI G'GATC_C
Cuts at: 0 797 1272
Size: 797 475
BanI G'GyrC_C
Cuts at: 0 606 731 1136 1272
Size: 606 125 405 136
Fragments arranged by size:
606 405 136 125
BanII G_rGCy'C
Cuts at: 0 395 592 844 859 1082 1272
Size: 395 197 252 15 223 190
Fragments arranged by size:
395 252 223 197 190 15
BbeI G_GCGC'C
Cuts at: 0 610 1272
Size: 610 662
BbsI GAAGACnn'nnnn_
Cuts at: 0 12 217 229 967 1272
Size: 12 205 12 738 305
Fragments arranged by size:
738 305 205 12 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 696 1272
Size: 696 576
BclI T'GATC_A
Cuts at: 0 486 1272
Size: 486 786
BlpI GC'TnA_GC
Cuts at: 0 591 1272
Size: 591 681
Bme1580I G_kGCm'C
Cuts at: 0 395 859 1272
Size: 395 464 413
Fragments arranged by size:
464 413 395
BmgBI CAC'GTC
Cuts at: 0 1148 1272
Size: 1148 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 708 914 1272
Size: 708 206 358
Fragments arranged by size:
708 358 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1272
Size: 1096 176
BsaI GGTCTCn'nnnn_
Cuts at: 0 544 1272
Size: 544 728
BsaAI yAC'GTr
Cuts at: 0 1064 1272
Size: 1064 208
BsaHI Gr'CG_yC
Cuts at: 0 146 607 1272
Size: 146 461 665
Fragments arranged by size:
665 461 146
BsaWI w'CCGG_w
Cuts at: 0 299 1272
Size: 299 973
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 562 1084 1255 1272
Size: 244 318 522 171 17
Fragments arranged by size:
522 318 244 171 17
BseYI C'CCAG_C
Cuts at: 0 30 837 1272
Size: 30 807 435
Fragments arranged by size:
807 435 30
BsiEI CG_ry'CG
Cuts at: 0 98 478 1160 1272
Size: 98 380 682 112
Fragments arranged by size:
682 380 112 98
BsiHKAI G_wGCw'C
Cuts at: 0 1082 1272
Size: 1082 190
BsmBI CGTCTCn'nnnn_
Cuts at: 0 573 743 1272
Size: 573 170 529
Fragments arranged by size:
573 529 170
Bsp1286I G_dGCh'C
Cuts at: 0 395 592 844 859 1082 1272
Size: 395 197 252 15 223 190
Fragments arranged by size:
395 252 223 197 190 15
BspEI T'CCGG_A
Cuts at: 0 299 1272
Size: 299 973
BspHI T'CATG_A
Cuts at: 0 956 1272
Size: 956 316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 743 1272
Size: 179 564 529
Fragments arranged by size:
564 529 179
BsrBI CCG'CTC
Cuts at: 0 474 1166 1272
Size: 474 692 106
Fragments arranged by size:
692 474 106
BsrFI r'CCGG_y
Cuts at: 0 968 1272
Size: 968 304
BssSI C'ACGA_G
Cuts at: 0 514 1272
Size: 514 758
BstYI r'GATC_y
Cuts at: 0 797 944 1118 1272
Size: 797 147 174 154
Fragments arranged by size:
797 174 154 147
BtgI C'CryG_G
Cuts at: 0 253 779 1272
Size: 253 526 493
Fragments arranged by size:
526 493 253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 360 1272
Size: 360 912
BtsI GCAGTG_nn'
Cuts at: 0 44 1272
Size: 44 1228
EaeI y'GGCC_r
Cuts at: 0 66 95 475 989 1157 1272
Size: 66 29 380 514 168 115
Fragments arranged by size:
514 380 168 115 66 29
EagI C'GGCC_G
Cuts at: 0 95 475 1157 1272
Size: 95 380 682 115
Fragments arranged by size:
682 380 115 95
EarI CTCTTCn'nnn_
Cuts at: 0 452 1013 1272
Size: 452 561 259
Fragments arranged by size:
561 452 259
EcoICRI GAG'CTC
Cuts at: 0 1080 1272
Size: 1080 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 241 1096 1272
Size: 229 12 855 176
Fragments arranged by size:
855 229 176 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 739 1272
Size: 739 533
EcoO109I rG'GnC_Cy
Cuts at: 0 392 449 855 856 1272
Size: 392 57 406 1 416
Fragments arranged by size:
416 406 392 57 1
FspI TGC'GCA
Cuts at: 0 175 1125 1272
Size: 175 950 147
Fragments arranged by size:
950 175 147
HaeII r_GCGC'y
Cuts at: 0 610 1272
Size: 610 662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 543 575 753 785 1153 1185
Size: 91 32 420 32 178 32 368 32
Cuts at: 1185 1272
Size: 87
Fragments arranged by size:
420 368 178 91 87 32 32 32
32
HincII GTy'rAC
Cuts at: 0 150 1272
Size: 150 1122
KasI G'GCGC_C
Cuts at: 0 606 1272
Size: 606 666
MscI TGG'CCA
Cuts at: 0 991 1272
Size: 991 281
NarI GG'CG_CC
Cuts at: 0 607 1272
Size: 607 665
NcoI C'CATG_G
Cuts at: 0 253 1272
Size: 253 1019
PflMI CCAn_nnn'nTGG
Cuts at: 0 972 1272
Size: 972 300
PfoI T'CCnGG_A
Cuts at: 0 806 1272
Size: 806 466
PmlI CAC'GTG
Cuts at: 0 1064 1272
Size: 1064 208
PpuMI rG'GwC_Cy
Cuts at: 0 449 1272
Size: 449 823
PspOMI G'GGCC_C
Cuts at: 0 391 855 1272
Size: 391 464 417
Fragments arranged by size:
464 417 391
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 355 387 1272
Size: 355 32 885
Fragments arranged by size:
885 355 32
RsrII CG'GwC_CG
Cuts at: 0 638 1272
Size: 638 634
SacI G_AGCT'C
Cuts at: 0 1082 1272
Size: 1082 190
SapI GCTCTTCn'nnn_
Cuts at: 0 1013 1272
Size: 1013 259
ScaI AGT'ACT
Cuts at: 0 520 1204 1272
Size: 520 684 68
Fragments arranged by size:
684 520 68
SexAI A'CCwGG_T
Cuts at: 0 293 404 1272
Size: 293 111 868
Fragments arranged by size:
868 293 111
SfcI C'TryA_G
Cuts at: 0 522 1272
Size: 522 750
SfoI GGC'GCC
Cuts at: 0 608 1272
Size: 608 664
StyI C'CwwG_G
Cuts at: 0 253 1272
Size: 253 1019
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 1272
Size: 225 1047
TatI w'GTAC_w
Cuts at: 0 497 518 1202 1272
Size: 497 21 684 70
Fragments arranged by size:
684 497 70 21
ZraI GAC'GTC
Cuts at: 0 147 1272
Size: 147 1125
Enzymes that do cut:
AarI AatII AcuI AhdI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BlpI Bme1580I
BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssSI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII KasI MscI NarI NcoI PflMI PfoI
PmlI PpuMI PspOMI PsrI RsrII SacI SapI ScaI
SexAI SfcI SfoI StyI TaqII TatI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BfrBI BglI BglII BmtI BplI
Bpu10I BpuEI BsaBI BsaXI BsgI BsiWI BsmI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KpnI MfeI MluI
MmeI MslI MspA1I NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PpiI
PshAI PsiI PspXI PstI PvuI PvuII SacII SalI
SanDI SbfI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI