(Circular) (Six-base) MAPSORT of: pFN21ASDA0955.seq Check: 7332 from: 1 to: 6022 pFN21ASDA0955 6022 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2493 3832 278 Size: 53 83 186 936 957 1339 2468 Fragments arranged by size: 2468 1339 957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3365 1937 Size: 1428 4594 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 3241 4085 5469 5737 1632 Size: 588 1021 844 1384 268 1917 Fragments arranged by size: 1917 1384 1021 844 588 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2627 1184 Size: 1443 4579 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2801 2833 2801 Size: 32 5990 AlwNI CAG_nnn'CTG Cuts at: 2191 2359 2392 3765 5337 2191 Size: 168 33 1373 1572 2876 Fragments arranged by size: 2876 1572 1373 168 33 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 2898 2915 3310 3331 3557 3709 5818 1493 Size: 1405 17 395 21 226 152 2109 1697 Fragments arranged by size: 2109 1697 1405 395 226 152 21 17 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2550 3347 1870 Size: 73 607 797 4545 Fragments arranged by size: 4545 797 607 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2982 3015 2982 Size: 33 5989 BamHI G'GATC_C Cuts at: 3352 3719 3352 Size: 367 5655 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3343 4798 618 Size: 325 217 627 1556 1455 1842 Fragments arranged by size: 1842 1556 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2891 3341 1391 Size: 1500 450 4072 Fragments arranged by size: 4072 1500 450 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5051 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4213 4247 1903 Size: 34 2276 34 3678 Fragments arranged by size: 3678 2276 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2983 3914 5124 2983 Size: 931 1210 3881 Fragments arranged by size: 3881 1210 931 BclI T'GATC_A Cuts at: 1548 1548 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4633 136 Size: 107 122 71 631 605 2961 1525 Fragments arranged by size: 2961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 3212 3439 3212 Size: 227 5795 Bme1580I G_kGCm'C Cuts at: 1790 4083 5239 1790 Size: 2293 1156 2573 Fragments arranged by size: 2573 2293 1156 BmgBI CAC'GTC Cuts at: 1318 3250 1318 Size: 1932 4090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3753 4711 442 Size: 416 53 83 399 24 2336 958 1753 Fragments arranged by size: 2336 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2368 2558 2913 2999 3113 4682 1239 Size: 1129 190 355 86 114 1569 2579 Fragments arranged by size: 2579 1569 1129 355 190 114 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2972 4147 5012 5310 2972 Size: 1175 865 298 3684 Fragments arranged by size: 3684 1175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4685 882 Size: 617 3186 2219 Fragments arranged by size: 3186 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3319 3813 493 Size: 711 2115 494 2702 Fragments arranged by size: 2702 2115 711 494 BsaBI GATnn'nnATC Cuts at: 2603 6016 2603 Size: 3413 2609 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2490 2724 3829 4211 Size: 53 83 186 936 957 234 1105 382 Cuts at: 4211 4846 275 Size: 635 1451 Fragments arranged by size: 1451 1105 957 936 635 382 234 186 83 53 BsaWI w'CCGG_w Cuts at: 4455 4853 5127 5274 5685 4455 Size: 398 274 147 411 4792 Fragments arranged by size: 4792 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2453 2483 1178 Size: 30 165 30 1050 30 4717 Fragments arranged by size: 4717 1050 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 3030 1186 Size: 1261 583 4178 Fragments arranged by size: 4178 1261 583 BseYI C'CCAG_C Cuts at: 2585 2717 2924 5225 5721 2585 Size: 132 207 2301 496 2886 Fragments arranged by size: 2886 2301 496 207 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3075 1163 Size: 573 1339 4110 Fragments arranged by size: 4110 1339 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3472 4233 5261 1540 Size: 275 186 1471 761 1028 2301 Fragments arranged by size: 2301 1471 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 3120 3255 4083 4168 5239 2237 Size: 883 135 828 85 1071 3020 Fragments arranged by size: 3020 1071 883 828 135 85 BsmI GAATG_Cn' Cuts at: 3533 3626 3533 Size: 93 5929 BsmBI CGTCTCn'nnnn_ Cuts at: 2497 2497 Size: 6022 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2891 3120 3255 3341 4083 4168 Size: 399 447 654 229 135 86 742 85 Cuts at: 4168 5239 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 654 447 399 229 135 86 85 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 3911 5641 3911 Size: 1730 4292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2379 3377 844 Size: 1535 998 3489 Fragments arranged by size: 3489 1535 998 BsrBI CCG'CTC Cuts at: 1394 1823 3909 1394 Size: 429 2086 3507 Fragments arranged by size: 3507 2086 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4517 4691 65 Size: 1183 3269 174 1396 Fragments arranged by size: 3269 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3078 4666 4853 1700 Size: 110 1268 1588 187 2869 Fragments arranged by size: 2869 1588 1268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6022 BssSI C'ACGA_G Cuts at: 3287 4082 5094 3287 Size: 795 1012 4215 Fragments arranged by size: 4215 1012 795 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 2007 4826 2007 Size: 2819 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6022 BstYI r'GATC_y Cuts at: 1972 3010 3352 3719 4104 4121 5573 5659 5671 Size: 1038 342 367 385 17 1452 86 12 Cuts at: 5671 6017 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1038 385 367 346 342 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2767 513 Size: 552 1702 3768 Fragments arranged by size: 3768 1702 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5283 Fragments arranged by size: 5283 683 56 BtsI GCAGTG_nn' Cuts at: 2262 2418 3538 4332 4352 2262 Size: 156 1120 794 20 3932 Fragments arranged by size: 3932 1120 794 156 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5029 809 Size: 507 3713 1802 Fragments arranged by size: 3713 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3469 4358 5987 Size: 54 1425 50 275 18 1639 889 1629 Cuts at: 5987 8 Size: 43 Fragments arranged by size: 1639 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3469 1537 Size: 275 1657 4090 Fragments arranged by size: 4090 1657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 3260 3952 1353 Size: 756 1151 692 3423 Fragments arranged by size: 3423 1151 756 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4609 4983 5129 387 Size: 1064 138 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 2368 2558 2913 2999 3113 3241 Size: 393 588 148 190 355 86 114 128 Cuts at: 3241 4085 4682 5469 5737 1239 Size: 844 597 787 268 1524 Fragments arranged by size: 1524 844 787 597 588 393 355 268 190 148 128 114 86 EcoNI CCTnn'n_nnAGG Cuts at: 3048 3048 Size: 6022 EcoO109I rG'GnC_Cy Cuts at: 2195 3103 3235 3277 2195 Size: 908 132 42 4940 Fragments arranged by size: 4940 908 132 42 EcoRI G'AATT_C Cuts at: 3331 3709 3331 Size: 378 5644 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2719 3297 2719 Size: 578 5444 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 5169 1678 Size: 3491 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2656 2688 4663 Size: 32 135 32 521 32 531 32 1975 Cuts at: 4663 4695 4737 4769 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1975 531 521 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2074 3366 3612 669 Size: 988 281 136 1292 246 3079 Fragments arranged by size: 3079 1292 988 281 246 136 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2679 5136 5320 1305 Size: 1374 2457 184 2007 Fragments arranged by size: 2457 2007 1374 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4200 Fragments arranged by size: 4200 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3980 4339 4498 518 Size: 648 2814 359 159 2042 Fragments arranged by size: 2814 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3438 4115 5263 5508 1606 Size: 1832 677 1148 245 2120 Fragments arranged by size: 2120 1832 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2767 513 Size: 552 1702 3768 Fragments arranged by size: 3768 1702 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 3380 4925 1132 Size: 97 2151 1545 2229 Fragments arranged by size: 2229 2151 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6022 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6022 PfoI T'CCnGG_A Cuts at: 2345 2535 2574 3129 2345 Size: 190 39 555 5238 Fragments arranged by size: 5238 555 190 39 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3103 3235 3277 3103 Size: 132 42 5848 Fragments arranged by size: 5848 132 42 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 2175 3592 2175 Size: 1417 4605 PstI C_TGCA'G Cuts at: 830 2376 2425 2784 3374 830 Size: 1546 49 359 590 3478 Fragments arranged by size: 3478 1546 590 359 49 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SanDI GG'GwC_CC Cuts at: 3277 3277 Size: 6022 SapI GCTCTTCn'nnn_ Cuts at: 3260 3260 Size: 6022 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SfcI C'TryA_G Cuts at: 826 1045 2372 2421 2780 2881 3370 4505 5186 Size: 219 1327 49 359 101 489 1135 681 Cuts at: 5186 5377 826 Size: 191 1471 Fragments arranged by size: 1471 1327 1135 681 489 359 219 191 101 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 2552 3349 1872 Size: 680 797 4545 Fragments arranged by size: 4545 797 680 SmlI C'TyrA_G Cuts at: 820 1017 1943 2951 4126 5027 5289 820 Size: 197 926 1008 1175 901 262 1553 Fragments arranged by size: 1553 1175 1008 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3319 3813 493 Size: 2826 494 2702 Fragments arranged by size: 2826 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 2295 3206 1517 Size: 778 911 4333 Fragments arranged by size: 4333 911 778 StyI C'CwwG_G Cuts at: 513 1065 2476 2767 2862 3461 513 Size: 552 1411 291 95 599 3074 Fragments arranged by size: 3074 1411 599 552 291 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4049 4066 4219 4404 4849 325 Size: 1255 2469 17 153 185 445 1498 Fragments arranged by size: 2469 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4268 5980 96 Size: 274 80 33 51 1443 2291 1712 138 Fragments arranged by size: 2291 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2453 2485 2608 2640 5982 6014 2453 Size: 32 123 32 3342 32 2461 Fragments arranged by size: 3342 2461 123 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 2550 3347 1870 Size: 680 797 4545 Fragments arranged by size: 4545 797 680 XmnI GAAnn'nnTTC Cuts at: 2943 4151 2943 Size: 1208 4814 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2491 3830 276 Size: 53 83 186 936 957 1339 2468 Fragments arranged by size: 2468 1339 957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI