(Circular) (Six-base) MAPSORT of: pFN21ASDA0952.seq Check: 8952 from: 1 to: 6295 pFN21ASDA0952 6295 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2518 3077 3638 1937 Size: 581 559 561 4594 Fragments arranged by size: 4594 581 561 559 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2922 3086 4358 5742 6010 1632 Size: 1290 164 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1290 1272 268 164 AfeI AGC'GCT Cuts at: 3143 3143 Size: 6295 AflII C'TTAA_G Cuts at: 820 1017 3305 820 Size: 197 2288 3810 Fragments arranged by size: 3810 2288 197 AflIII A'CryG_T Cuts at: 1203 5194 6171 1203 Size: 3991 977 1327 Fragments arranged by size: 3991 1327 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2171 1184 Size: 987 5308 AlwNI CAG_nnn'CTG Cuts at: 2655 4038 5610 2655 Size: 1383 1572 3340 Fragments arranged by size: 3340 1572 1383 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 3604 3830 3982 6091 1493 Size: 2111 226 152 2109 1697 Fragments arranged by size: 2111 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 3620 1870 Size: 73 1677 4545 Fragments arranged by size: 4545 1677 73 BamHI G'GATC_C Cuts at: 3625 3992 3625 Size: 367 5928 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3616 5071 618 Size: 325 217 627 1829 1455 1842 Fragments arranged by size: 1842 1829 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2462 3614 1391 Size: 1071 1152 4072 Fragments arranged by size: 4072 1152 1071 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2195 928 Size: 971 296 5028 Fragments arranged by size: 5028 971 296 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2519 2553 2993 3027 4486 4520 1903 Size: 34 582 34 440 34 1459 34 3678 Fragments arranged by size: 3678 1459 582 440 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4187 5397 4187 Size: 1210 5085 BclI T'GATC_A Cuts at: 1548 1548 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4906 136 Size: 107 122 71 631 605 3234 1525 Fragments arranged by size: 3234 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2923 6290 2923 Size: 3367 2928 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 4356 5512 1790 Size: 2566 1156 2573 Fragments arranged by size: 2573 2566 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2905 3389 3555 Size: 416 53 83 399 24 1488 484 166 Cuts at: 3555 4026 4984 442 Size: 471 958 1753 Fragments arranged by size: 1753 1488 958 484 471 416 399 166 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2230 2690 3250 4955 1239 Size: 991 460 560 1705 2579 Fragments arranged by size: 2579 1705 991 560 460 Bpu10I CC'TnA_GC Cuts at: 3096 3096 Size: 6295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2774 3358 4420 5285 5583 2774 Size: 584 1062 865 298 3486 Fragments arranged by size: 3486 1062 865 584 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2666 3047 4958 882 Size: 617 1167 381 1911 2219 Fragments arranged by size: 2219 1911 1167 617 381 BsaAI yAC'GTr Cuts at: 493 1204 3592 4086 493 Size: 711 2388 494 2702 Fragments arranged by size: 2702 2388 711 494 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4102 4484 5119 275 Size: 53 83 186 936 2569 382 635 1451 Fragments arranged by size: 2569 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6070 Fragments arranged by size: 6070 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2696 2744 3514 1186 Size: 1510 48 770 3967 Fragments arranged by size: 3967 1510 770 48 BseYI C'CCAG_C Cuts at: 2745 2754 3138 5498 5994 2745 Size: 9 384 2360 496 3046 Fragments arranged by size: 3046 2360 496 384 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3009 3419 1163 Size: 573 1273 410 4039 Fragments arranged by size: 4039 1273 573 410 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2462 2706 3524 4356 4441 5512 2462 Size: 244 818 832 85 1071 3245 Fragments arranged by size: 3245 1071 832 818 244 85 BsmI GAATG_Cn' Cuts at: 2698 2863 2915 3806 3899 2698 Size: 165 52 891 93 5094 Fragments arranged by size: 5094 891 165 93 52 BsmBI CGTCTCn'nnnn_ Cuts at: 2075 2075 Size: 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2462 2706 3524 3614 4356 4441 5512 Size: 399 672 244 818 90 742 85 1071 Cuts at: 5512 1391 Size: 2174 Fragments arranged by size: 2174 1071 818 742 672 399 244 90 85 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2542 3650 844 Size: 1698 1108 3489 Fragments arranged by size: 3489 1698 1108 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3240 3454 4790 4964 65 Size: 1183 1992 214 1336 174 1396 Fragments arranged by size: 1992 1396 1336 1183 214 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4939 5126 1700 Size: 110 3129 187 2869 Fragments arranged by size: 3129 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2386 96 Size: 2290 4005 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 3020 794 Size: 2226 4069 BstBI TT'CG_AA Cuts at: 2007 5099 2007 Size: 3092 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6295 BstYI r'GATC_y Cuts at: 1972 2923 3564 3625 3992 4377 4394 5846 5932 Size: 951 641 61 367 385 17 1452 86 Cuts at: 5932 5944 6290 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 951 641 385 367 346 86 61 17 12 BstZ17I GTA'TAC Cuts at: 2519 3078 2519 Size: 559 5736 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4786 Fragments arranged by size: 4786 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2369 2489 487 Size: 683 56 1143 120 4293 Fragments arranged by size: 4293 1143 683 120 56 BtsI GCAGTG_nn' Cuts at: 3811 4605 4625 3811 Size: 794 20 5481 Fragments arranged by size: 5481 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DraIII CAC_nnn'GTG Cuts at: 2411 2411 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2604 3742 4631 Size: 54 1425 50 275 18 774 1138 889 Cuts at: 4631 6260 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1138 889 774 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4225 1353 Size: 2872 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2460 2460 Size: 6295 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2230 2690 2922 3086 3250 4358 4955 Size: 393 598 460 232 164 164 1108 597 Cuts at: 4955 5742 6010 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1108 787 598 597 460 393 268 232 164 164 EcoNI CCTnn'n_nnAGG Cuts at: 2726 2726 Size: 6295 EcoO109I rG'GnC_Cy Cuts at: 3127 3127 Size: 6295 EcoRI G'AATT_C Cuts at: 3604 3982 3604 Size: 378 5917 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 3145 3190 5442 1678 Size: 1467 45 2252 2531 Fragments arranged by size: 2531 2252 1467 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3549 3581 4936 4968 5010 Size: 32 135 32 1977 32 1355 32 42 Cuts at: 5010 5042 1373 Size: 32 2626 Fragments arranged by size: 2626 1977 1355 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3639 3885 669 Size: 988 281 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6295 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5409 5593 1305 Size: 4104 184 2007 Fragments arranged by size: 4104 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2606 10 Size: 54 1425 343 774 3699 Fragments arranged by size: 3699 1425 774 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2439 4253 4612 4771 518 Size: 648 1273 1814 359 159 2042 Fragments arranged by size: 2042 1814 1273 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3270 3711 4388 5536 5781 1606 Size: 1664 441 677 1148 245 2120 Fragments arranged by size: 2120 1664 1148 677 441 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4786 Fragments arranged by size: 4786 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 3653 5198 1132 Size: 97 2424 1545 2229 Fragments arranged by size: 2424 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6295 PciI A'CATG_T Cuts at: 5194 5194 Size: 6295 PflMI CCAn_nnn'nTGG Cuts at: 1108 2411 1108 Size: 1303 4992 PfoI T'CCnGG_A Cuts at: 2667 2667 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PstI C_TGCA'G Cuts at: 830 3085 3283 3647 830 Size: 2255 198 364 3478 Fragments arranged by size: 3478 2255 364 198 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 PvuII CAG'CTG Cuts at: 3270 3270 Size: 6295 SacI G_AGCT'C Cuts at: 2462 2462 Size: 6295 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 3347 4543 6255 3347 Size: 1196 1712 3387 Fragments arranged by size: 3387 1712 1196 SexAI A'CCwGG_T Cuts at: 2407 2407 Size: 6295 SfcI C'TryA_G Cuts at: 826 1045 3081 3279 3643 4778 5459 5650 826 Size: 219 2036 198 364 1135 681 191 1471 Fragments arranged by size: 2036 1471 1135 681 364 219 198 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2789 3305 3337 4399 5300 5562 Size: 197 926 846 516 32 1062 901 262 Cuts at: 5562 820 Size: 1553 Fragments arranged by size: 1553 1062 926 901 846 516 262 197 32 SnaBI TAC'GTA Cuts at: 493 3592 4086 493 Size: 3099 494 2702 Fragments arranged by size: 3099 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 3653 1132 Size: 2521 3774 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6295 StyI C'CwwG_G Cuts at: 513 1065 2022 3734 513 Size: 552 957 1712 3074 Fragments arranged by size: 3074 1712 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4322 4339 4492 4677 5122 325 Size: 1255 2742 17 153 185 445 1498 Fragments arranged by size: 2742 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2386 2628 3345 Size: 274 80 33 51 1443 409 242 717 Cuts at: 3345 4541 6253 96 Size: 1196 1712 138 Fragments arranged by size: 1712 1443 1196 717 409 274 242 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6255 6287 6255 Size: 32 6263 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2869 3436 2869 Size: 567 5728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 2835 4424 2835 Size: 1589 4706 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI