Restriction map for
pFN21ASDA0952
(Linear) (Six-base) MAPSORT of: KIAA0952.seq Check: 2052 from: 1 to: 1566
KIAA0952.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 495 1054 1566
Size: 495 559 512
Fragments arranged by size:
559 512 495
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1063 1566
Size: 899 164 503
Fragments arranged by size:
899 503 164
AfeI AGC'GCT
Cuts at: 0 1120 1566
Size: 1120 446
AflII C'TTAA_G
Cuts at: 0 1282 1566
Size: 1282 284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 1566
Size: 148 1418
AlwNI CAG_nnn'CTG
Cuts at: 0 632 1566
Size: 632 934
BanII G_rGCy'C
Cuts at: 0 439 1566
Size: 439 1127
BbsI GAAGACnn'nnnn_
Cuts at: 0 172 1566
Size: 172 1394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 496 530 970 1004 1566
Size: 496 34 440 34 562
Fragments arranged by size:
562 496 440 34 34
BglII A'GATC_T
Cuts at: 0 900 1566
Size: 900 666
BmrI ACTGGGnnnn_n'
Cuts at: 0 882 1366 1532 1566
Size: 882 484 166 34
Fragments arranged by size:
882 484 166 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 667 1227 1566
Size: 207 460 560 339
Fragments arranged by size:
560 460 339 207
Bpu10I CC'TnA_GC
Cuts at: 0 1073 1566
Size: 1073 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1335 1566
Size: 751 584 231
Fragments arranged by size:
751 584 231
BsaI GGTCTCn'nnnn_
Cuts at: 0 643 1024 1566
Size: 643 381 542
Fragments arranged by size:
643 542 381
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 721 1491 1566
Size: 673 48 770 75
Fragments arranged by size:
770 673 75 48
BseYI C'CCAG_C
Cuts at: 0 722 731 1115 1566
Size: 722 9 384 451
Fragments arranged by size:
722 451 384 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1396 1566
Size: 986 410 170
Fragments arranged by size:
986 410 170
BsiHKAI G_wGCw'C
Cuts at: 0 439 683 1501 1566
Size: 439 244 818 65
Fragments arranged by size:
818 439 244 65
BsmI GAATG_Cn'
Cuts at: 0 675 840 892 1566
Size: 675 165 52 674
Fragments arranged by size:
675 674 165 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 52 1566
Size: 52 1514
Bsp1286I G_dGCh'C
Cuts at: 0 439 683 1501 1566
Size: 439 244 818 65
Fragments arranged by size:
818 439 244 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 519 1566
Size: 519 1047
BsrDI GCAATG_nn'
Cuts at: 0 1217 1431 1566
Size: 1217 214 135
Fragments arranged by size:
1217 214 135
BsrGI T'GTAC_A
Cuts at: 0 363 1566
Size: 363 1203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 997 1566
Size: 997 569
BstYI r'GATC_y
Cuts at: 0 900 1541 1566
Size: 900 641 25
Fragments arranged by size:
900 641 25
BstZ17I GTA'TAC
Cuts at: 0 496 1055 1566
Size: 496 559 511
Fragments arranged by size:
559 511 496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 346 466 1566
Size: 346 120 1100
Fragments arranged by size:
1100 346 120
DraIII CAC_nnn'GTG
Cuts at: 0 388 1566
Size: 388 1178
EaeI y'GGCC_r
Cuts at: 0 581 1566
Size: 581 985
EcoICRI GAG'CTC
Cuts at: 0 437 1566
Size: 437 1129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 667 899 1063 1227 1566
Size: 207 460 232 164 164 339
Fragments arranged by size:
460 339 232 207 164 164
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 1566
Size: 703 863
EcoO109I rG'GnC_Cy
Cuts at: 0 1104 1566
Size: 1104 462
HaeII r_GCGC'y
Cuts at: 0 1122 1167 1566
Size: 1122 45 399
Fragments arranged by size:
1122 399 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1526 1558 1566
Size: 1526 32 8
Fragments arranged by size:
1526 32 8
MscI TGG'CCA
Cuts at: 0 583 1566
Size: 583 983
MslI CAynn'nnrTG
Cuts at: 0 416 1566
Size: 416 1150
MspA1I CmG'CkG
Cuts at: 0 1247 1566
Size: 1247 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 388 1566
Size: 388 1178
PfoI T'CCnGG_A
Cuts at: 0 644 1566
Size: 644 922
PstI C_TGCA'G
Cuts at: 0 1062 1260 1566
Size: 1062 198 306
Fragments arranged by size:
1062 306 198
PvuII CAG'CTG
Cuts at: 0 1247 1566
Size: 1247 319
SacI G_AGCT'C
Cuts at: 0 439 1566
Size: 439 1127
ScaI AGT'ACT
Cuts at: 0 1324 1566
Size: 1324 242
SexAI A'CCwGG_T
Cuts at: 0 384 1566
Size: 384 1182
SfcI C'TryA_G
Cuts at: 0 1058 1256 1566
Size: 1058 198 310
Fragments arranged by size:
1058 310 198
SmlI C'TyrA_G
Cuts at: 0 766 1282 1314 1566
Size: 766 516 32 252
Fragments arranged by size:
766 516 252 32
TatI w'GTAC_w
Cuts at: 0 363 605 1322 1566
Size: 363 242 717 244
Fragments arranged by size:
717 363 244 242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 1413 1566
Size: 846 567 153
Fragments arranged by size:
846 567 153
XmnI GAAnn'nnTTC
Cuts at: 0 812 1566
Size: 812 754
Enzymes that do cut:
AccI AcuI AfeI AflII AlfI AlwNI BanII BbsI
BcgI BglII BmrI BpmI Bpu10I BpuEI BsaI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrGI BstAPI BstYI BstZ17I BtgZI DraIII EaeI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I
PflMI PfoI PstI PvuII SacI ScaI SexAI SfcI
SmlI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI Bsu36I
BtgI BtsI ClaI CspCI DraI DrdI EagI EarI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI