(Circular) (Six-base) MAPSORT of: pFN21ASDA0941.seq Check: 3418 from: 1 to: 6265 pFN21ASDA0941 6265 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2311 2311 Size: 6265 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 2117 3586 2117 Size: 1469 4796 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2117 2765 4328 5712 5980 1632 Size: 485 648 1563 1384 268 1917 Fragments arranged by size: 1917 1563 1384 648 485 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 3400 4008 5580 3400 Size: 608 1572 4085 Fragments arranged by size: 4085 1572 608 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2309 2618 2963 3031 3254 3545 3574 3800 Size: 816 309 345 68 223 291 29 226 Cuts at: 3800 3952 6061 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 816 345 309 291 226 223 152 68 29 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3452 3590 1870 Size: 73 1509 138 4545 Fragments arranged by size: 4545 1509 138 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 2753 3586 5041 618 Size: 325 217 627 330 636 833 1455 1842 Fragments arranged by size: 1842 1455 833 636 627 330 325 217 BanII G_rGCy'C Cuts at: 1391 3584 1391 Size: 2193 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2565 2724 2923 3321 928 Size: 971 666 159 199 398 3872 Fragments arranged by size: 3872 971 666 398 199 159 BbvCI CC'TCA_GC Cuts at: 2679 2679 Size: 6265 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BfrBI ATG'CAT Cuts at: 3194 3194 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4876 136 Size: 107 122 71 631 605 3204 1525 Fragments arranged by size: 3204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2092 2504 3134 6260 2092 Size: 412 630 3126 2097 Fragments arranged by size: 3126 2097 630 412 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 4326 5482 1790 Size: 2536 1156 2573 Fragments arranged by size: 2573 2536 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 3996 4954 Size: 416 53 83 399 24 2078 501 958 Cuts at: 4954 442 Size: 1753 Fragments arranged by size: 2078 1753 958 501 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4925 1239 Size: 3686 2579 Bpu10I CC'TnA_GC Cuts at: 2679 2679 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 4390 5255 5553 2210 Size: 2180 865 298 2922 Fragments arranged by size: 2922 2180 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2065 3562 4056 493 Size: 711 861 1497 494 2702 Fragments arranged by size: 2702 1497 861 711 494 BsaBI GATnn'nnATC Cuts at: 2389 6259 2389 Size: 3870 2395 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2491 2521 1178 Size: 30 165 30 1088 30 4922 Fragments arranged by size: 4922 1088 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 1186 Size: 1036 5229 BseYI C'CCAG_C Cuts at: 2146 5468 5964 2146 Size: 3322 496 2447 Fragments arranged by size: 3322 2447 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 1163 Size: 573 351 5341 Fragments arranged by size: 5341 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 4326 4411 5482 2082 Size: 2244 85 1071 2865 Fragments arranged by size: 2865 2244 1071 85 BsmI GAATG_Cn' Cuts at: 2698 3776 3869 2698 Size: 1078 93 5094 Fragments arranged by size: 5094 1078 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 3584 4326 4411 5482 1391 Size: 399 292 1502 742 85 1071 2174 Fragments arranged by size: 2174 1502 1071 742 399 292 85 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 2311 3620 844 Size: 1215 252 1309 3489 Fragments arranged by size: 3489 1309 1215 252 BsrBI CCG'CTC Cuts at: 1394 1823 4152 1394 Size: 429 2329 3507 Fragments arranged by size: 3507 2329 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3316 4760 4934 65 Size: 1183 2068 1444 174 1396 Fragments arranged by size: 2068 1444 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4909 5096 1700 Size: 110 3099 187 2869 Fragments arranged by size: 3099 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 2007 5069 2007 Size: 3062 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2092 2504 3134 3158 3595 3962 4347 4364 Size: 120 412 630 24 437 367 385 17 Cuts at: 4364 5816 5902 5914 6260 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 630 437 412 385 367 346 120 86 24 17 12 Bsu36I CC'TnA_GG Cuts at: 3320 3422 3320 Size: 102 6163 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5713 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 2972 3354 3781 4575 4595 2972 Size: 382 427 794 20 4642 Fragments arranged by size: 4642 794 427 382 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3712 4601 6230 Size: 54 1425 50 275 18 1882 889 1629 Cuts at: 6230 8 Size: 43 Fragments arranged by size: 1882 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3378 4195 1353 Size: 2025 817 3423 Fragments arranged by size: 3423 2025 817 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2765 4328 4925 5712 5980 1239 Size: 393 485 648 1563 597 787 268 1524 Fragments arranged by size: 1563 1524 787 648 597 485 393 268 EcoO109I rG'GnC_Cy Cuts at: 2289 2675 2721 2289 Size: 386 46 5833 Fragments arranged by size: 5833 386 46 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3437 3437 Size: 6265 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 5412 1678 Size: 3734 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3167 3199 3215 3247 4906 Size: 32 135 32 1595 32 16 32 1659 Cuts at: 4906 4938 4980 5012 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1659 1595 135 42 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3609 3855 669 Size: 988 281 1296 375 246 3079 Fragments arranged by size: 3079 1296 988 375 281 246 HindIII A'AGCT_T Cuts at: 748 2851 748 Size: 2103 4162 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 2121 3590 2121 Size: 1469 4796 MfeI C'AATT_G Cuts at: 3048 3864 3048 Size: 816 5449 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2081 3326 5379 5563 1305 Size: 776 1245 2053 184 2007 Fragments arranged by size: 2053 2007 1245 776 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4443 Fragments arranged by size: 4443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4223 4582 4741 518 Size: 648 3057 359 159 2042 Fragments arranged by size: 3057 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 3681 4358 5506 5751 1606 Size: 540 1535 677 1148 245 2120 Fragments arranged by size: 2120 1535 1148 677 540 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5713 NdeI CA'TA_TG Cuts at: 387 2727 3294 3520 387 Size: 2340 567 226 3132 Fragments arranged by size: 3132 2340 567 226 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NsiI A_TGCA'T Cuts at: 3196 3196 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2602 3623 5168 1132 Size: 97 1373 1021 1545 2229 Fragments arranged by size: 2229 1545 1373 1021 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 2816 1108 Size: 1708 4557 PfoI T'CCnGG_A Cuts at: 3330 3444 3530 3330 Size: 114 86 6065 Fragments arranged by size: 6065 114 86 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PmlI CAC'GTG Cuts at: 2065 2065 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2289 2675 2721 2289 Size: 386 46 5833 Fragments arranged by size: 5833 386 46 PshAI GACnn'nnGTC Cuts at: 1613 2689 1613 Size: 1076 5189 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspXI vC'TCGA_Gb Cuts at: 3452 3452 Size: 6265 PstI C_TGCA'G Cuts at: 830 3361 3617 830 Size: 2531 256 3478 Fragments arranged by size: 3478 2531 256 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2146 2146 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SanDI GG'GwC_CC Cuts at: 2721 2721 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 2162 2593 4513 6225 2162 Size: 431 1920 1712 2202 Fragments arranged by size: 2202 1920 1712 431 SfcI C'TryA_G Cuts at: 826 1045 2172 3357 3392 3613 4748 5429 5620 Size: 219 1127 1185 35 221 1135 681 191 Cuts at: 5620 826 Size: 1471 Fragments arranged by size: 1471 1185 1135 1127 681 221 219 191 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 3452 4369 5270 5532 820 Size: 197 926 246 1263 917 901 262 1553 Fragments arranged by size: 1553 1263 926 917 901 262 246 197 SnaBI TAC'GTA Cuts at: 493 3562 4056 493 Size: 3069 494 2702 Fragments arranged by size: 3069 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 2440 4189 5 Size: 47 2388 1749 2081 Fragments arranged by size: 2388 2081 1749 47 StuI AGG'CCT Cuts at: 1517 2213 1517 Size: 696 5569 StyI C'CwwG_G Cuts at: 513 1065 2978 3704 513 Size: 552 1913 726 3074 Fragments arranged by size: 3074 1913 726 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3452 4292 4309 4462 4647 5092 325 Size: 1255 1872 840 17 153 185 445 1498 Fragments arranged by size: 1872 1498 1255 840 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2160 2591 4511 Size: 274 80 33 51 1443 183 431 1920 Cuts at: 4511 6223 96 Size: 1712 138 Fragments arranged by size: 1920 1712 1443 431 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 3452 1943 Size: 1509 4756 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 2560 3387 4394 2560 Size: 827 1007 4431 Fragments arranged by size: 4431 1007 827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI