Restriction map for
pFN21ASDA0932
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# NO CUT ENZYMES ( 82 enzymes) #
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AccI AclI AcsI AflII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI AspEI AspI AvrII
BamHI BbsI BbvCI BclI BfaI
BfrI BglI BglII BlnI BpuAI
BsaBI BsmI BspDI BspHI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KspI MaeI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 2011 GACGTC GACGT^C
Acc65I 1109 GGTACC G^GTACC
AflIII 783 ACATGT A^C[GA][CT]GT
Asp718I 1109 GGTACC G^GTACC
AviII 2039 TGCGCA TGC^GCA
BbrPI 1501 CACGTG CAC^GTG
BcgI 1542 CGACAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1917 GTATCC GTATCC(6/5)
BlpI 2876 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2876 GCTCAGC GC^T[ACGT]AGC
BsiEI 2892 CGGCCG CG[GA][CT]^CG
BsiWI 1376 CGTACG C^GTACG
Bsp1407I 1034 TGTACA T^GTACA
BspLU11I 783 ACATGT A^CATGT
BsrBI 1958 CCGCTC CCGCTC(-3/-3)
BsrGI 1034 TGTACA T^GTACA
BstEII 1146 GGTCACC G^GT[ACGT]ACC
BstPI 1146 GGTCACC G^GT[ACGT]ACC
CelII 2876 GCTCAGC GC^T[ACGT]AGC
DraI 1195 TTTAAA TTT^AAA
EagI 2892 CGGCCG C^GGCCG
EclXI 2892 CGGCCG C^GGCCG
Eco52I 2892 CGGCCG C^GGCCG
EcoO65I 1146 GGTCACC G^GT[ACGT]ACC
FspI 2039 TGCGCA TGC^GCA
HincII 2242 GTCAAC GT[CT]^[GA]AC
HindII 2242 GTCAAC GT[CT]^[GA]AC
KpnI 1109 GGTACC GGTAC^C
NaeI 1316 GCCGGC GCC^GGC
NdeI 1553 CATATG CA^TATG
NgoMIV 1316 GCCGGC G^CCGGC
PmaCI 1501 CACGTG CAC^GTG
PmlI 1501 CACGTG CAC^GTG
SbfI 1062 CCTGCAGG CCTGCA^GG
SexAI 1092 ACCTGGT A^CC[AT]GGT
SphI 408 GCATGC GCATG^C
Sse8387I 1062 CCTGCAGG CCTGCA^GG
SspBI 1034 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccIII 1440 TCCGGA T^CCGGA
2804 TCCGGA
Alw44I 2327 GTGCAC G^TGCAC
2650 GTGCAC
ApaI 23 GGGCCC GGGCC^C
2177 GGGCCC
ApaLI 2327 GTGCAC G^TGCAC
2650 GTGCAC
BalI 984 TGGCCA TGG^CCA
1744 TGGCCA
BbeI 64 GGCGCC GGCGC^C
656 GGCGCC
BsaAI 1222 TACGTG [CT]AC^GT[GA]
1501 CACGTG
BsaI 776 GAGACC GGTCTC(1/5)
1057 GAGACC
BseAI 1440 TCCGGA T^CCGGA
2804 TCCGGA
BspEI 1440 TCCGGA T^CCGGA
2804 TCCGGA
BsrDI 192 CATTGC GCAATG(2/0)
2006 GCAATG
BsrFI 1316 GCCGGC [GA]^CCGG[CT]
1414 GCCGGT
BssSI 724 CACGAG CACGAG(-5/-1)
2293 CACGAG
BstAPI 2075 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2657 GCACAGAGTGC
Cfr10I 1316 GCCGGC [GA]^CCGG[CT]
1414 GCCGGT
DraIII 2434 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2658 CACAGAGTG
HindIII 606 AAGCTT A^AGCTT
1520 AAGCTT
KasI 64 GGCGCC G^GCGCC
656 GGCGCC
MluNI 984 TGGCCA TGG^CCA
1744 TGGCCA
MroI 1440 TCCGGA T^CCGGA
2804 TCCGGA
MscI 984 TGGCCA TGG^CCA
1744 TGGCCA
NarI 64 GGCGCC GG^CGCC
656 GGCGCC
NcoI 332 CCATGG C^CATGG
1184 CCATGG
PleI 74 GACTC GAGTC(4/5)
485 GAGTC
PpuMI 400 GGGACCT [GA]G^G[AT]CC[CT]
2353 GGGACCC
PvuII 142 CAGCTG CAG^CTG
1545 CAGCTG
SapI 295 GAAGAGC GCTCTTC(1/4)
443 GAAGAGC
SfoI 64 GGCGCC GGC^GCC
656 GGCGCC
SmaI 5 CCCGGG CCC^GGG
434 CCCGGG
Tsp509I 829 AATT ^AATT
1714 AATT
XcmI 251 CCAAGCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2712 CCACGCCCAGTTTGG
XmaCI 5 CCCGGG C^CCGGG
434 CCCGGG
XmaI 5 CCCGGG C^CCGGG
434 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Asp700I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XhoII XmnI