Restriction map for
pFN21ASDA0932
(Linear) (Six-base) MAPSORT of: KIAA0932.seq Check: 8476 from: 1 to: 3045
KIAA0932.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1935 2258 2926 3045
Size: 1935 323 668 119
Fragments arranged by size:
1935 668 323 119
AatII G_ACGT'C
Cuts at: 0 2015 3045
Size: 2015 1030
Acc65I G'GTAC_C
Cuts at: 0 1109 3045
Size: 1109 1936
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1963 2157 2698 2705 3045
Size: 1963 194 541 7 340
Fragments arranged by size:
1963 541 340 194 7
AflIII A'CryG_T
Cuts at: 0 783 3045
Size: 783 2262
AleI CACnn'nnGTG
Cuts at: 0 572 574 1613 3045
Size: 572 2 1039 1432
Fragments arranged by size:
1432 1039 572 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2143 3045
Size: 2143 902
AlwNI CAG_nnn'CTG
Cuts at: 0 144 245 632 2081 3045
Size: 144 101 387 1449 964
Fragments arranged by size:
1449 964 387 144 101
ApaI G_GGCC'C
Cuts at: 0 27 2181 3045
Size: 27 2154 864
Fragments arranged by size:
2154 864 27
ApaLI G'TGCA_C
Cuts at: 0 2327 2650 3045
Size: 2327 323 395
Fragments arranged by size:
2327 395 323
AvaI C'yCGr_G
Cuts at: 0 5 76 434 2739 3045
Size: 5 71 358 2305 306
Fragments arranged by size:
2305 358 306 71 5
BanI G'GyrC_C
Cuts at: 0 64 343 656 1109 3045
Size: 64 279 313 453 1936
Fragments arranged by size:
1936 453 313 279 64
BanII G_rGCy'C
Cuts at: 0 27 862 1298 1651 2181 2368 2705 3045
Size: 27 835 436 353 530 187 337 340
Fragments arranged by size:
835 530 436 353 340 337 187 27
BbeI G_GCGC'C
Cuts at: 0 68 660 3045
Size: 68 592 2385
Fragments arranged by size:
2385 592 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1531 1565 3045
Size: 1531 34 1480
Fragments arranged by size:
1531 1480 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1928 3045
Size: 1928 1117
BlpI GC'TnA_GC
Cuts at: 0 2877 3045
Size: 2877 168
Bme1580I G_kGCm'C
Cuts at: 0 27 133 1599 2181 2331 2654 3045
Size: 27 106 1466 582 150 323 391
Fragments arranged by size:
1466 582 391 323 150 106 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 527 567 2712 2769 3045
Size: 527 40 2145 57 276
Fragments arranged by size:
2145 527 276 57 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2461 3045
Size: 2461 584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 500 1155 1266 1494 3045
Size: 166 334 655 111 228 1551
Fragments arranged by size:
1551 655 334 228 166 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 903 1004 3045
Size: 441 462 101 2041
Fragments arranged by size:
2041 462 441 101
BsaI GGTCTCn'nnnn_
Cuts at: 0 770 1051 3045
Size: 770 281 1994
Fragments arranged by size:
1994 770 281
BsaAI yAC'GTr
Cuts at: 0 1224 1503 3045
Size: 1224 279 1542
Fragments arranged by size:
1542 1224 279
BsaHI Gr'CG_yC
Cuts at: 0 65 92 657 968 2012 2060 2095 3045
Size: 65 27 565 311 1044 48 35 950
Fragments arranged by size:
1044 950 565 311 65 48 35 27
BsaWI w'CCGG_w
Cuts at: 0 1440 1967 2804 3045
Size: 1440 527 837 241
Fragments arranged by size:
1440 837 527 241
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 414 444 1957 1987 2128 2143 2158 2173
Size: 414 30 1513 30 141 15 15 15
Cuts at: 2173 2388 2418 3045
Size: 215 30 627
Fragments arranged by size:
1513 627 414 215 141 30 30 30
15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 497 677 680 938 2836 2839 3045
Size: 139 358 180 3 258 1898 3 206
Fragments arranged by size:
1898 358 258 206 180 139 3 3
BseYI C'CCAG_C
Cuts at: 0 729 847 1044 1785 2389 3045
Size: 729 118 197 741 604 656
Fragments arranged by size:
741 729 656 604 197 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1073 2053 2286 2292 2647 2710 3016
Size: 373 700 980 233 6 355 63 306
Cuts at: 3016 3045
Size: 29
Fragments arranged by size:
980 700 373 355 306 233 63 29
6
BsiEI CG_ry'CG
Cuts at: 0 2895 3045
Size: 2895 150
BsiHKAI G_wGCw'C
Cuts at: 0 862 1651 2331 2654 2705 3045
Size: 862 789 680 323 51 340
Fragments arranged by size:
862 789 680 340 323 51
BsiWI C'GTAC_G
Cuts at: 0 1376 3045
Size: 1376 1669
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1308 2086 2163 3045
Size: 1308 778 77 882
Fragments arranged by size:
1308 882 778 77
Bsp1286I G_dGCh'C
Cuts at: 0 27 133 862 1298 1599 1651 2181 2331
Size: 27 106 729 436 301 52 530 150
Cuts at: 2331 2368 2654 2705 3045
Size: 37 286 51 340
Fragments arranged by size:
729 530 436 340 301 286 150 106
52 51 37 27
BspEI T'CCGG_A
Cuts at: 0 1440 2804 3045
Size: 1440 1364 241
Fragments arranged by size:
1440 1364 241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1259 1396 1935 2066 2258 2926 3045
Size: 1259 137 539 131 192 668 119
Fragments arranged by size:
1259 668 539 192 137 131 119
BsrBI CCG'CTC
Cuts at: 0 1960 3045
Size: 1960 1085
BsrDI GCAATG_nn'
Cuts at: 0 191 2013 3045
Size: 191 1822 1032
Fragments arranged by size:
1822 1032 191
BsrFI r'CCGG_y
Cuts at: 0 1316 1414 3045
Size: 1316 98 1631
Fragments arranged by size:
1631 1316 98
BsrGI T'GTAC_A
Cuts at: 0 1034 3045
Size: 1034 2011
BssSI C'ACGA_G
Cuts at: 0 724 2293 3045
Size: 724 1569 752
Fragments arranged by size:
1569 752 724
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2081 2663 3045
Size: 2081 582 382
Fragments arranged by size:
2081 582 382
BstEII G'GTnAC_C
Cuts at: 0 1146 3045
Size: 1146 1899
BstXI CCAn_nnnn'nTGG
Cuts at: 0 333 742 1615 2299 3045
Size: 333 409 873 684 746
Fragments arranged by size:
873 746 684 409 333
BstYI r'GATC_y
Cuts at: 0 1139 1286 1970 3045
Size: 1139 147 684 1075
Fragments arranged by size:
1139 1075 684 147
BtgI C'CryG_G
Cuts at: 0 332 1184 1307 1583 2573 3045
Size: 332 852 123 276 990 472
Fragments arranged by size:
990 852 472 332 276 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1753 3045
Size: 1753 1292
BtsI GCAGTG_nn'
Cuts at: 0 34 1125 2349 2654 3045
Size: 34 1091 1224 305 391
Fragments arranged by size:
1224 1091 391 305 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 145 180 3045
Size: 145 35 2865
Fragments arranged by size:
2865 145 35
DraI TTT'AAA
Cuts at: 0 1197 3045
Size: 1197 1848
DraIII CAC_nnn'GTG
Cuts at: 0 2439 2663 3045
Size: 2439 224 382
Fragments arranged by size:
2439 382 224
DrdI GACnn_nn'nnGTC
Cuts at: 0 784 967 2155 3045
Size: 784 183 1188 890
Fragments arranged by size:
1188 890 784 183
EaeI y'GGCC_r
Cuts at: 0 366 766 984 1605 1744 1826 1906 2892
Size: 366 400 218 621 139 82 80 986
Cuts at: 2892 3045
Size: 153
Fragments arranged by size:
986 621 400 366 218 153 139 82
80
EagI C'GGCC_G
Cuts at: 0 2892 3045
Size: 2892 153
EarI CTCTTCn'nnn_
Cuts at: 0 290 438 1583 2424 3045
Size: 290 148 1145 841 621
Fragments arranged by size:
1145 841 621 290 148
EcoICRI GAG'CTC
Cuts at: 0 860 1649 2703 3045
Size: 860 789 1054 342
Fragments arranged by size:
1054 860 789 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 500 1155 1266 1494 1963 2157 2698
Size: 166 334 655 111 228 469 194 541
Cuts at: 2698 2705 3045
Size: 7 340
Fragments arranged by size:
655 541 469 340 334 228 194 166
111 7
EcoO109I rG'GnC_Cy
Cuts at: 0 23 24 401 2177 2354 3045
Size: 23 1 377 1776 177 691
Fragments arranged by size:
1776 691 377 177 23 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1276 1383 2043 2187 3045
Size: 1276 107 660 144 858
Fragments arranged by size:
1276 858 660 144 107
FspI TGC'GCA
Cuts at: 0 2041 3045
Size: 2041 1004
HaeII r_GCGC'y
Cuts at: 0 68 87 660 2884 3045
Size: 68 19 573 2224 161
Fragments arranged by size:
2224 573 161 68 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 414 446 473 505 1413 1445
Size: 289 32 93 32 27 32 908 32
Cuts at: 1445 1627 1659 2128 2160 3045
Size: 182 32 469 32 885
Fragments arranged by size:
908 885 469 289 182 93 32 32
32 32 32 32 27
HincII GTy'rAC
Cuts at: 0 2244 3045
Size: 2244 801
HindIII A'AGCT_T
Cuts at: 0 606 1520 3045
Size: 606 914 1525
Fragments arranged by size:
1525 914 606
KasI G'GCGC_C
Cuts at: 0 64 656 3045
Size: 64 592 2389
Fragments arranged by size:
2389 592 64
KpnI G_GTAC'C
Cuts at: 0 1113 3045
Size: 1113 1932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1676 2171 3045
Size: 1676 495 874
Fragments arranged by size:
1676 874 495
MscI TGG'CCA
Cuts at: 0 986 1746 3045
Size: 986 760 1299
Fragments arranged by size:
1299 986 760
MslI CAynn'nnrTG
Cuts at: 0 331 572 574 1183 1613 1772 2131 2297
Size: 331 241 2 609 430 159 359 166
Cuts at: 2297 3045
Size: 748
Fragments arranged by size:
748 609 430 359 331 241 166 159
2
MspA1I CmG'CkG
Cuts at: 0 54 144 175 1547 2045 2753 3045
Size: 54 90 31 1372 498 708 292
Fragments arranged by size:
1372 708 498 292 90 54 31
NaeI GCC'GGC
Cuts at: 0 1318 3045
Size: 1318 1727
NarI GG'CG_CC
Cuts at: 0 65 657 3045
Size: 65 592 2388
Fragments arranged by size:
2388 592 65
NcoI C'CATG_G
Cuts at: 0 332 1184 3045
Size: 332 852 1861
Fragments arranged by size:
1861 852 332
NdeI CA'TA_TG
Cuts at: 0 1554 3045
Size: 1554 1491
NgoMIV G'CCGG_C
Cuts at: 0 1316 3045
Size: 1316 1729
NspI r_CATG'y
Cuts at: 0 412 787 2130 3045
Size: 412 375 1343 915
Fragments arranged by size:
1343 915 412 375
PasI CC'CwG_GG
Cuts at: 0 397 1153 1900 3045
Size: 397 756 747 1145
Fragments arranged by size:
1145 756 747 397
PciI A'CATG_T
Cuts at: 0 783 3045
Size: 783 2262
PflMI CCAn_nnn'nTGG
Cuts at: 0 245 982 1051 3045
Size: 245 737 69 1994
Fragments arranged by size:
1994 737 245 69
PfoI T'CCnGG_A
Cuts at: 0 1232 1289 1571 3020 3045
Size: 1232 57 282 1449 25
Fragments arranged by size:
1449 1232 282 57 25
PmlI CAC'GTG
Cuts at: 0 1503 3045
Size: 1503 1542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 901 933 3045
Size: 901 32 2112
Fragments arranged by size:
2112 901 32
PpuMI rG'GwC_Cy
Cuts at: 0 401 2354 3045
Size: 401 1953 691
Fragments arranged by size:
1953 691 401
PspOMI G'GGCC_C
Cuts at: 0 23 2177 3045
Size: 23 2154 868
Fragments arranged by size:
2154 868 23
PstI C_TGCA'G
Cuts at: 0 1067 2430 2937 3045
Size: 1067 1363 507 108
Fragments arranged by size:
1363 1067 507 108
PvuII CAG'CTG
Cuts at: 0 144 1547 3045
Size: 144 1403 1498
Fragments arranged by size:
1498 1403 144
SacI G_AGCT'C
Cuts at: 0 862 1651 2705 3045
Size: 862 789 1054 340
Fragments arranged by size:
1054 862 789 340
SanDI GG'GwC_CC
Cuts at: 0 2354 3045
Size: 2354 691
SapI GCTCTTCn'nnn_
Cuts at: 0 290 438 3045
Size: 290 148 2607
Fragments arranged by size:
2607 290 148
SbfI CC_TGCA'GG
Cuts at: 0 1067 3045
Size: 1067 1978
SexAI A'CCwGG_T
Cuts at: 0 1092 3045
Size: 1092 1953
SfcI C'TryA_G
Cuts at: 0 1063 1449 2426 2933 3045
Size: 1063 386 977 507 112
Fragments arranged by size:
1063 977 507 386 112
SfoI GGC'GCC
Cuts at: 0 66 658 3045
Size: 66 592 2387
Fragments arranged by size:
2387 592 66
SmaI CCC'GGG
Cuts at: 0 7 436 3045
Size: 7 429 2609
Fragments arranged by size:
2609 429 7
SmlI C'TyrA_G
Cuts at: 0 456 918 1019 3045
Size: 456 462 101 2026
Fragments arranged by size:
2026 462 456 101
SphI G_CATG'C
Cuts at: 0 412 3045
Size: 412 2633
StyI C'CwwG_G
Cuts at: 0 332 347 1184 1460 2210 3045
Size: 332 15 837 276 750 835
Fragments arranged by size:
837 835 750 332 276 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1134 1314 1892 2751 3045
Size: 1134 180 578 859 294
Fragments arranged by size:
1134 859 578 294 180
TatI w'GTAC_w
Cuts at: 0 1034 3045
Size: 1034 2011
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 684 716 930 962 1082 1114 3045
Size: 684 32 214 32 120 32 1931
Fragments arranged by size:
1931 684 214 120 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 2719 3045
Size: 258 2461 326
Fragments arranged by size:
2461 326 258
XmaI C'CCGG_G
Cuts at: 0 5 434 3045
Size: 5 429 2611
Fragments arranged by size:
2611 429 5
XmnI GAAnn'nnTTC
Cuts at: 0 913 1083 2638 3045
Size: 913 170 1555 407
Fragments arranged by size:
1555 913 407 170
ZraI GAC'GTC
Cuts at: 0 2013 3045
Size: 2013 1032
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BcgI BciVI
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI
SphI StyI TaqII TatI TstI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BclI BfrBI BglI BglII BmgBI BmtI Bpu10I BsaBI
BsmI BspHI BssHII BstBI BstZ17I Bsu36I ClaI EciI
EcoNI EcoRI EcoRV FseI FspAI HpaI MfeI MluI
NheI NotI NruI NsiI PacI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XhoI