(Circular) (Six-base) MAPSORT of: pFN21ASDA0924.seq Check: 5788 from: 1 to: 6547 pFN21ASDA0924 6547 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3535 3678 3535 Size: 143 6404 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3890 1937 Size: 1953 4594 Acc65I G'GTAC_C Cuts at: 2796 3868 2796 Size: 1072 5475 AclI AA'CG_TT Cuts at: 1563 2817 4674 5047 1563 Size: 1254 1857 373 3063 Fragments arranged by size: 3063 1857 1254 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4610 5994 6262 1632 Size: 2978 1384 268 1917 Fragments arranged by size: 2978 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2462 3473 820 Size: 197 1445 1011 3894 Fragments arranged by size: 3894 1445 1011 197 AflIII A'CryG_T Cuts at: 1203 5446 6423 1203 Size: 4243 977 1327 Fragments arranged by size: 4243 1327 977 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 5276 5276 Size: 6547 AleI CACnn'nnGTG Cuts at: 1166 2168 1166 Size: 1002 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6547 AlwNI CAG_nnn'CTG Cuts at: 2053 2285 4290 5862 2053 Size: 232 2005 1572 2738 Fragments arranged by size: 2738 2005 1572 232 ApaLI G'TGCA_C Cuts at: 3143 4604 5760 3143 Size: 1461 1156 3930 Fragments arranged by size: 3930 1461 1156 ApoI r'AATT_y Cuts at: 1493 2317 3005 3018 3032 3856 4082 4234 6343 Size: 824 688 13 14 824 226 152 2109 Cuts at: 6343 1493 Size: 1697 Fragments arranged by size: 2109 1697 824 824 688 226 152 14 13 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 3872 1870 Size: 73 1929 4545 Fragments arranged by size: 4545 1929 73 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2796 3804 3868 5323 Size: 325 217 627 351 658 1008 64 1455 Cuts at: 5323 618 Size: 1842 Fragments arranged by size: 1842 1455 1008 658 627 351 325 217 64 BanII G_rGCy'C Cuts at: 1391 2712 3866 1391 Size: 1321 1154 4072 Fragments arranged by size: 4072 1321 1154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2313 928 Size: 971 414 5162 Fragments arranged by size: 5162 971 414 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4738 4772 1903 Size: 34 2801 34 3678 Fragments arranged by size: 3678 2801 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4439 5649 4439 Size: 1210 5337 BclI T'GATC_A Cuts at: 1548 1548 Size: 6547 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5158 136 Size: 107 122 71 631 605 3486 1525 Fragments arranged by size: 3486 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6542 6542 Size: 6547 BlpI GC'TnA_GC Cuts at: 3964 3964 Size: 6547 Bme1580I G_kGCm'C Cuts at: 1790 2774 3147 3252 4608 5764 1790 Size: 984 373 105 1356 1156 2573 Fragments arranged by size: 2573 1356 1156 984 373 105 BmgBI CAC'GTC Cuts at: 1318 2290 1318 Size: 972 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3560 4278 5236 Size: 416 53 83 399 24 2143 718 958 Cuts at: 5236 442 Size: 1753 Fragments arranged by size: 2143 1753 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2822 3183 3351 3702 3726 5207 1239 Size: 1583 361 168 351 24 1481 2579 Fragments arranged by size: 2579 1583 1481 361 351 168 24 Bpu10I CC'TnA_GC Cuts at: 2682 2682 Size: 6547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3122 4672 5537 5835 3122 Size: 1550 865 298 3834 Fragments arranged by size: 3834 1550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5210 882 Size: 617 3711 2219 Fragments arranged by size: 3711 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3844 4338 493 Size: 711 2640 494 2702 Fragments arranged by size: 2702 2640 711 494 BsaBI GATnn'nnATC Cuts at: 6541 6541 Size: 6547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4354 4736 5371 275 Size: 53 83 186 936 2821 382 635 1451 Fragments arranged by size: 2821 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4980 5378 5652 5799 6210 4980 Size: 398 274 147 411 5317 Fragments arranged by size: 5317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2220 2250 1178 Size: 30 165 30 817 30 5475 Fragments arranged by size: 5475 817 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2492 1186 Size: 1162 144 5241 Fragments arranged by size: 5241 1162 144 BseYI C'CCAG_C Cuts at: 3556 5750 6246 3556 Size: 2194 496 3857 Fragments arranged by size: 3857 2194 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3656 1163 Size: 573 1920 4054 Fragments arranged by size: 4054 1920 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3997 4758 5786 1540 Size: 275 186 1996 761 1028 2301 Fragments arranged by size: 2301 1996 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3147 4608 4693 5764 3147 Size: 1461 85 1071 3930 Fragments arranged by size: 3930 1461 1071 85 BsmI GAATG_Cn' Cuts at: 3157 3237 4058 4151 3157 Size: 80 821 93 5553 Fragments arranged by size: 5553 821 93 80 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2712 2774 3147 3252 3866 4608 4693 Size: 399 922 62 373 105 614 742 85 Cuts at: 4693 5764 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 922 742 614 399 373 105 85 62 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 2965 4436 6166 2965 Size: 1471 1730 3346 Fragments arranged by size: 3346 1730 1471 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3535 3678 3902 844 Size: 2691 143 224 3489 Fragments arranged by size: 3489 2691 224 143 BsrBI CCG'CTC Cuts at: 1394 1823 4434 1394 Size: 429 2611 3507 Fragments arranged by size: 3507 2611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2527 5042 5216 65 Size: 1183 1279 2515 174 1396 Fragments arranged by size: 2515 1396 1279 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5191 5378 1700 Size: 110 3381 187 2869 Fragments arranged by size: 3381 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2172 96 Size: 2076 4471 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 3151 794 Size: 2357 4190 BstBI TT'CG_AA Cuts at: 2007 5351 2007 Size: 3344 3203 BstEII G'GTnAC_C Cuts at: 1184 3502 1184 Size: 2318 4229 BstXI CCAn_nnnn'nTGG Cuts at: 3275 3275 Size: 6547 BstYI r'GATC_y Cuts at: 1972 3537 3877 4244 4629 4646 6098 6184 6196 Size: 1565 340 367 385 17 1452 86 12 Cuts at: 6196 6542 1972 Size: 346 1977 Fragments arranged by size: 1977 1565 1452 385 367 346 340 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2986 3042 513 Size: 552 1921 56 4018 Fragments arranged by size: 4018 1921 552 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5808 Fragments arranged by size: 5808 683 56 BtsI GCAGTG_nn' Cuts at: 2286 2479 4063 4857 4877 2286 Size: 193 1584 794 20 3956 Fragments arranged by size: 3956 1584 794 193 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6512 DraI TTT'AAA Cuts at: 3768 3851 3768 Size: 83 6464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3445 3994 4883 Size: 54 1425 50 275 18 1615 549 889 Cuts at: 4883 6512 8 Size: 1629 43 Fragments arranged by size: 1629 1615 1425 889 549 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3994 1537 Size: 275 2182 4090 Fragments arranged by size: 4090 2182 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 2440 2478 2484 2496 2660 2736 3386 Size: 981 106 38 6 12 164 76 650 Cuts at: 3386 3639 4477 1353 Size: 253 838 3423 Fragments arranged by size: 3423 981 838 650 253 164 106 76 38 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5134 5508 5654 387 Size: 1064 138 3545 374 146 1280 Fragments arranged by size: 3545 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2822 3183 3351 3702 3726 4610 5207 Size: 393 1190 361 168 351 24 884 597 Cuts at: 5207 5994 6262 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1190 884 787 597 393 361 351 268 168 24 EcoRI G'AATT_C Cuts at: 2317 3856 4234 2317 Size: 1539 378 4630 Fragments arranged by size: 4630 1539 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3319 3319 Size: 6547 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 5694 1678 Size: 4016 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5188 5220 5262 5294 1373 Size: 32 135 32 3616 32 42 32 2626 Fragments arranged by size: 3616 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3891 4137 669 Size: 988 281 1953 246 3079 Fragments arranged by size: 3079 1953 988 281 246 HindIII A'AGCT_T Cuts at: 748 3367 748 Size: 2619 3928 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 2800 3872 2800 Size: 1072 5475 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5661 5845 1305 Size: 4356 184 2007 Fragments arranged by size: 4356 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3447 10 Size: 54 1425 343 1615 3110 Fragments arranged by size: 3110 1615 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2219 2238 2991 3094 4505 4864 Size: 648 1002 51 19 753 103 1411 359 Cuts at: 4864 5023 518 Size: 159 2042 Fragments arranged by size: 2042 1411 1002 753 648 359 159 103 51 19 MspA1I CmG'CkG Cuts at: 1606 3451 3963 4640 5788 6033 1606 Size: 1845 512 677 1148 245 2120 Fragments arranged by size: 2120 1845 1148 677 512 245 NcoI C'CATG_G Cuts at: 513 1065 2986 3042 513 Size: 552 1921 56 4018 Fragments arranged by size: 4018 1921 552 56 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 2078 2297 2836 3088 3099 3472 3905 Size: 97 849 219 539 252 11 373 433 Cuts at: 3905 5450 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 849 539 433 373 252 219 97 11 PasI CC'CwG_GG Cuts at: 1348 2775 1348 Size: 1427 5120 PciI A'CATG_T Cuts at: 5446 5446 Size: 6547 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6547 PfoI T'CCnGG_A Cuts at: 2868 2868 Size: 6547 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PstI C_TGCA'G Cuts at: 830 3899 830 Size: 3069 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SbfI CC_TGCA'GG Cuts at: 3899 3899 Size: 6547 ScaI AGT'ACT Cuts at: 3302 4795 6507 3302 Size: 1493 1712 3342 Fragments arranged by size: 3342 1712 1493 SfcI C'TryA_G Cuts at: 826 1045 3895 5030 5711 5902 826 Size: 219 2850 1135 681 191 1471 Fragments arranged by size: 2850 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 3874 1872 Size: 2002 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2462 3137 3473 4651 5552 5814 Size: 197 926 519 675 336 1178 901 262 Cuts at: 5814 820 Size: 1553 Fragments arranged by size: 1553 1178 926 901 675 519 336 262 197 SnaBI TAC'GTA Cuts at: 493 3844 4338 493 Size: 3351 494 2702 Fragments arranged by size: 3351 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 2297 2836 3905 1132 Size: 1165 539 1069 3774 Fragments arranged by size: 3774 1165 1069 539 SspI AAT'ATT Cuts at: 5 52 4471 5 Size: 47 4419 2081 Fragments arranged by size: 4419 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6547 StyI C'CwwG_G Cuts at: 513 1065 2986 3042 3986 513 Size: 552 1921 56 944 3074 Fragments arranged by size: 3074 1921 944 552 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3696 3711 4574 4591 4744 4929 5374 Size: 1255 2116 15 863 17 153 185 445 Cuts at: 5374 325 Size: 1498 Fragments arranged by size: 2116 1498 1255 863 445 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 3207 3300 Size: 274 80 33 51 1443 195 1035 93 Cuts at: 3300 4793 6505 96 Size: 1493 1712 138 Fragments arranged by size: 1712 1493 1443 1035 274 195 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3516 3548 3553 3585 6507 6539 3516 Size: 32 5 32 2922 32 3524 Fragments arranged by size: 3524 2922 32 32 32 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6547 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6547 XmaI C'CCGG_G Cuts at: 1870 3872 1870 Size: 2002 4545 XmnI GAAnn'nnTTC Cuts at: 3032 3201 4676 3032 Size: 169 1475 4903 Fragments arranged by size: 4903 1475 169 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI